Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02190 and RR42_RS02195 are separated by 2 nucleotides RR42_RS02195 and RR42_RS02200 are separated by 41 nucleotides RR42_RS02200 and RR42_RS02205 are separated by 65 nucleotides RR42_RS02205 and RR42_RS02210 overlap by 4 nucleotides RR42_RS02210 and RR42_RS02215 overlap by 4 nucleotides
RR42_RS02190: RR42_RS02190 - fumarate hydrolyase, at 487,601 to 488,215
_RS02190
RR42_RS02195: RR42_RS02195 - fumarate hydratase, at 488,218 to 489,093
_RS02195
RR42_RS02200: RR42_RS02200 - LacI family transcriptional regulator, at 489,135 to 490,130
_RS02200
RR42_RS02205: RR42_RS02205 - succinate dehydrogenase, at 490,196 to 490,549
_RS02205
RR42_RS02210: RR42_RS02210 - succinate dehydrogenase, at 490,546 to 490,899
_RS02210
RR42_RS02215: RR42_RS02215 - fumarate reductase, at 490,896 to 491,609
_RS02215
Position (kb) 489
490
491 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 488.206 kb on - strand at 488.206 kb on - strand at 488.206 kb on - strand at 488.314 kb on - strand, within RR42_RS02195 at 488.332 kb on + strand, within RR42_RS02195 at 488.335 kb on - strand, within RR42_RS02195 at 488.818 kb on + strand, within RR42_RS02195 at 488.819 kb on - strand, within RR42_RS02195 at 488.876 kb on + strand, within RR42_RS02195 at 488.877 kb on - strand, within RR42_RS02195 at 489.047 kb on + strand at 489.048 kb on - strand at 489.207 kb on + strand at 489.208 kb on - strand at 489.560 kb on - strand, within RR42_RS02200 at 489.560 kb on - strand, within RR42_RS02200 at 489.759 kb on - strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.845 kb on + strand, within RR42_RS02200 at 489.846 kb on - strand, within RR42_RS02200 at 489.846 kb on - strand, within RR42_RS02200 at 489.848 kb on - strand, within RR42_RS02200 at 489.848 kb on - strand, within RR42_RS02200 at 490.197 kb on + strand at 490.197 kb on + strand at 490.510 kb on - strand, within RR42_RS02205 at 490.566 kb on - strand at 490.856 kb on + strand, within RR42_RS02210 at 490.857 kb on - strand, within RR42_RS02210 at 490.900 kb on - strand at 490.900 kb on - strand at 490.900 kb on - strand at 491.003 kb on + strand, within RR42_RS02215 at 491.004 kb on - strand, within RR42_RS02215
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 488,206 - -0.2 488,206 - +0.8 488,206 - +1.1 488,314 - RR42_RS02195 0.11 -0.6 488,332 + RR42_RS02195 0.13 -0.1 488,335 - RR42_RS02195 0.13 -0.5 488,818 + RR42_RS02195 0.68 -1.1 488,819 - RR42_RS02195 0.69 -0.1 488,876 + RR42_RS02195 0.75 -0.8 488,877 - RR42_RS02195 0.75 +0.4 489,047 + -0.7 489,048 - +3.0 489,207 + +0.3 489,208 - -1.2 489,560 - RR42_RS02200 0.43 -0.8 489,560 - RR42_RS02200 0.43 -0.5 489,759 - RR42_RS02200 0.63 -1.8 489,845 + RR42_RS02200 0.71 +1.5 489,845 + RR42_RS02200 0.71 -0.3 489,845 + RR42_RS02200 0.71 -1.7 489,846 - RR42_RS02200 0.71 +1.1 489,846 - RR42_RS02200 0.71 +1.6 489,848 - RR42_RS02200 0.72 -1.2 489,848 - RR42_RS02200 0.72 -0.5 490,197 + -0.6 490,197 + -0.8 490,510 - RR42_RS02205 0.89 -0.4 490,566 - -0.3 490,856 + RR42_RS02210 0.88 +1.4 490,857 - RR42_RS02210 0.88 +1.1 490,900 - +0.7 490,900 - +0.1 490,900 - +0.4 491,003 + RR42_RS02215 0.15 +0.0 491,004 - RR42_RS02215 0.15 +0.3
Or see this region's nucleotide sequence