Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02185 and RR42_RS02190 overlap by 4 nucleotides RR42_RS02190 and RR42_RS02195 are separated by 2 nucleotides RR42_RS02195 and RR42_RS02200 are separated by 41 nucleotides
RR42_RS02185: RR42_RS02185 - fumarate reductase, at 485,877 to 487,604
_RS02185
RR42_RS02190: RR42_RS02190 - fumarate hydrolyase, at 487,601 to 488,215
_RS02190
RR42_RS02195: RR42_RS02195 - fumarate hydratase, at 488,218 to 489,093
_RS02195
RR42_RS02200: RR42_RS02200 - LacI family transcriptional regulator, at 489,135 to 490,130
_RS02200
Position (kb) 487
488
489 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 486.673 kb on + strand, within RR42_RS02185 at 486.674 kb on - strand, within RR42_RS02185 at 486.674 kb on - strand, within RR42_RS02185 at 486.718 kb on + strand, within RR42_RS02185 at 486.719 kb on - strand, within RR42_RS02185 at 486.719 kb on - strand, within RR42_RS02185 at 486.770 kb on - strand, within RR42_RS02185 at 486.988 kb on + strand, within RR42_RS02185 at 486.988 kb on + strand, within RR42_RS02185 at 486.989 kb on - strand, within RR42_RS02185 at 486.989 kb on - strand, within RR42_RS02185 at 487.675 kb on - strand, within RR42_RS02190 at 487.675 kb on - strand, within RR42_RS02190 at 487.791 kb on - strand, within RR42_RS02190 at 487.837 kb on - strand, within RR42_RS02190 at 487.907 kb on + strand, within RR42_RS02190 at 487.907 kb on + strand, within RR42_RS02190 at 487.908 kb on - strand, within RR42_RS02190 at 488.095 kb on + strand, within RR42_RS02190 at 488.095 kb on + strand, within RR42_RS02190 at 488.096 kb on - strand, within RR42_RS02190 at 488.096 kb on - strand, within RR42_RS02190 at 488.131 kb on + strand, within RR42_RS02190 at 488.206 kb on - strand at 488.206 kb on - strand at 488.206 kb on - strand at 488.314 kb on - strand, within RR42_RS02195 at 488.332 kb on + strand, within RR42_RS02195 at 488.335 kb on - strand, within RR42_RS02195 at 488.818 kb on + strand, within RR42_RS02195 at 488.819 kb on - strand, within RR42_RS02195 at 488.876 kb on + strand, within RR42_RS02195 at 488.877 kb on - strand, within RR42_RS02195 at 489.047 kb on + strand at 489.048 kb on - strand at 489.207 kb on + strand at 489.208 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 486,673 + RR42_RS02185 0.46 -0.1 486,674 - RR42_RS02185 0.46 -0.1 486,674 - RR42_RS02185 0.46 -0.2 486,718 + RR42_RS02185 0.49 +0.2 486,719 - RR42_RS02185 0.49 -0.7 486,719 - RR42_RS02185 0.49 -2.1 486,770 - RR42_RS02185 0.52 -0.2 486,988 + RR42_RS02185 0.64 +0.0 486,988 + RR42_RS02185 0.64 -1.9 486,989 - RR42_RS02185 0.64 +0.1 486,989 - RR42_RS02185 0.64 -0.1 487,675 - RR42_RS02190 0.12 -0.1 487,675 - RR42_RS02190 0.12 -1.0 487,791 - RR42_RS02190 0.31 +1.6 487,837 - RR42_RS02190 0.38 -1.7 487,907 + RR42_RS02190 0.50 +0.1 487,907 + RR42_RS02190 0.50 -0.1 487,908 - RR42_RS02190 0.50 -0.3 488,095 + RR42_RS02190 0.80 -0.8 488,095 + RR42_RS02190 0.80 +1.4 488,096 - RR42_RS02190 0.80 +0.3 488,096 - RR42_RS02190 0.80 -0.5 488,131 + RR42_RS02190 0.86 +0.6 488,206 - -0.2 488,206 - +0.8 488,206 - +1.1 488,314 - RR42_RS02195 0.11 -0.6 488,332 + RR42_RS02195 0.13 -0.1 488,335 - RR42_RS02195 0.13 -0.5 488,818 + RR42_RS02195 0.68 -1.1 488,819 - RR42_RS02195 0.69 -0.1 488,876 + RR42_RS02195 0.75 -0.8 488,877 - RR42_RS02195 0.75 +0.4 489,047 + -0.7 489,048 - +3.0 489,207 + +0.3 489,208 - -1.2
Or see this region's nucleotide sequence