Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_432

Experiment: nitrate 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_430 and Pf6N2E2_431 are separated by 3 nucleotidesPf6N2E2_431 and Pf6N2E2_432 are separated by 1 nucleotidesPf6N2E2_432 and Pf6N2E2_433 are separated by 128 nucleotides Pf6N2E2_430 - Molybdenum transport ATP-binding protein ModC (TC 3.A.1.8.1), at 497,691 to 498,770 _430 Pf6N2E2_431 - Molybdenum transport system permease protein ModB (TC 3.A.1.8.1), at 498,774 to 499,454 _431 Pf6N2E2_432 - Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1), at 499,456 to 500,208 _432 Pf6N2E2_433 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 500,337 to 501,410 _433 Position (kb) 499 500 501Strain fitness (log2 ratio) -2 -1 0 1at 498.532 kb on + strand, within Pf6N2E2_430at 498.533 kb on - strand, within Pf6N2E2_430at 498.533 kb on - strand, within Pf6N2E2_430at 498.609 kb on - strand, within Pf6N2E2_430at 498.662 kb on + strand, within Pf6N2E2_430at 498.683 kb on - strandat 498.809 kb on - strandat 498.875 kb on - strand, within Pf6N2E2_431at 498.899 kb on - strand, within Pf6N2E2_431at 498.899 kb on - strand, within Pf6N2E2_431at 498.899 kb on - strand, within Pf6N2E2_431at 499.150 kb on + strand, within Pf6N2E2_431at 499.150 kb on + strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.151 kb on - strand, within Pf6N2E2_431at 499.261 kb on + strand, within Pf6N2E2_431at 499.262 kb on - strand, within Pf6N2E2_431at 499.262 kb on - strand, within Pf6N2E2_431at 499.262 kb on - strand, within Pf6N2E2_431at 499.353 kb on + strand, within Pf6N2E2_431at 499.354 kb on - strand, within Pf6N2E2_431at 499.432 kb on + strandat 499.433 kb on - strandat 499.457 kb on + strandat 499.511 kb on + strandat 499.511 kb on + strandat 499.512 kb on - strandat 499.640 kb on + strand, within Pf6N2E2_432at 499.640 kb on + strand, within Pf6N2E2_432at 499.640 kb on + strand, within Pf6N2E2_432at 499.868 kb on + strand, within Pf6N2E2_432at 499.868 kb on + strand, within Pf6N2E2_432at 499.868 kb on + strand, within Pf6N2E2_432at 499.868 kb on + strand, within Pf6N2E2_432at 499.868 kb on + strand, within Pf6N2E2_432at 499.869 kb on - strand, within Pf6N2E2_432at 499.869 kb on - strand, within Pf6N2E2_432at 499.911 kb on - strand, within Pf6N2E2_432at 499.911 kb on - strand, within Pf6N2E2_432at 500.021 kb on + strand, within Pf6N2E2_432at 500.021 kb on + strand, within Pf6N2E2_432at 500.021 kb on + strand, within Pf6N2E2_432at 500.058 kb on + strand, within Pf6N2E2_432at 500.058 kb on + strand, within Pf6N2E2_432at 500.160 kb on + strandat 500.160 kb on + strandat 500.160 kb on + strandat 500.161 kb on - strandat 500.208 kb on + strandat 500.208 kb on + strandat 500.208 kb on + strandat 500.208 kb on + strandat 500.208 kb on + strandat 500.208 kb on + strandat 500.209 kb on - strandat 500.209 kb on - strandat 500.209 kb on - strandat 500.209 kb on - strandat 500.235 kb on + strandat 500.235 kb on + strandat 500.237 kb on + strandat 500.238 kb on - strandat 500.238 kb on - strandat 500.240 kb on - strandat 500.247 kb on + strandat 500.248 kb on - strandat 500.249 kb on + strandat 500.249 kb on + strandat 500.250 kb on - strandat 500.250 kb on - strandat 500.250 kb on - strandat 500.251 kb on + strandat 500.251 kb on + strandat 500.252 kb on - strandat 500.677 kb on + strand, within Pf6N2E2_433at 500.870 kb on - strand, within Pf6N2E2_433at 501.122 kb on - strand, within Pf6N2E2_433at 501.122 kb on - strand, within Pf6N2E2_433at 501.122 kb on - strand, within Pf6N2E2_433at 501.122 kb on - strand, within Pf6N2E2_433

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrate 200 mM
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498,532 + Pf6N2E2_430 0.78 -0.7
498,533 - Pf6N2E2_430 0.78 -0.6
498,533 - Pf6N2E2_430 0.78 +0.1
498,609 - Pf6N2E2_430 0.85 -0.8
498,662 + Pf6N2E2_430 0.90 -0.6
498,683 - +0.3
498,809 - -1.4
498,875 - Pf6N2E2_431 0.15 -1.1
498,899 - Pf6N2E2_431 0.18 -0.0
498,899 - Pf6N2E2_431 0.18 +1.1
498,899 - Pf6N2E2_431 0.18 +0.3
499,150 + Pf6N2E2_431 0.55 -0.9
499,150 + Pf6N2E2_431 0.55 -0.0
499,151 - Pf6N2E2_431 0.55 -1.0
499,151 - Pf6N2E2_431 0.55 +0.2
499,151 - Pf6N2E2_431 0.55 -0.4
499,151 - Pf6N2E2_431 0.55 +0.7
499,151 - Pf6N2E2_431 0.55 -0.1
499,151 - Pf6N2E2_431 0.55 -1.2
499,261 + Pf6N2E2_431 0.72 -0.2
499,262 - Pf6N2E2_431 0.72 +0.3
499,262 - Pf6N2E2_431 0.72 -0.1
499,262 - Pf6N2E2_431 0.72 -0.6
499,353 + Pf6N2E2_431 0.85 +0.1
499,354 - Pf6N2E2_431 0.85 -0.6
499,432 + -0.7
499,433 - -0.3
499,457 + +0.3
499,511 + -0.4
499,511 + +0.6
499,512 - +0.4
499,640 + Pf6N2E2_432 0.24 -0.6
499,640 + Pf6N2E2_432 0.24 -0.4
499,640 + Pf6N2E2_432 0.24 +0.1
499,868 + Pf6N2E2_432 0.55 -1.0
499,868 + Pf6N2E2_432 0.55 +0.8
499,868 + Pf6N2E2_432 0.55 -1.6
499,868 + Pf6N2E2_432 0.55 -0.2
499,868 + Pf6N2E2_432 0.55 -1.4
499,869 - Pf6N2E2_432 0.55 -0.2
499,869 - Pf6N2E2_432 0.55 -0.5
499,911 - Pf6N2E2_432 0.60 +0.1
499,911 - Pf6N2E2_432 0.60 -0.1
500,021 + Pf6N2E2_432 0.75 +1.1
500,021 + Pf6N2E2_432 0.75 -0.9
500,021 + Pf6N2E2_432 0.75 -1.0
500,058 + Pf6N2E2_432 0.80 +0.0
500,058 + Pf6N2E2_432 0.80 -0.5
500,160 + -0.5
500,160 + -0.8
500,160 + -2.1
500,161 - +0.2
500,208 + -0.1
500,208 + +0.1
500,208 + -0.3
500,208 + +0.8
500,208 + -0.0
500,208 + -0.9
500,209 - -0.8
500,209 - +0.3
500,209 - +0.4
500,209 - +1.3
500,235 + -0.5
500,235 + +0.3
500,237 + +0.6
500,238 - +0.7
500,238 - +0.4
500,240 - +0.3
500,247 + +0.4
500,248 - +1.1
500,249 + -1.0
500,249 + -0.2
500,250 - -0.7
500,250 - +0.3
500,250 - -0.3
500,251 + +0.3
500,251 + +1.4
500,252 - -0.3
500,677 + Pf6N2E2_433 0.32 +0.7
500,870 - Pf6N2E2_433 0.50 +0.2
501,122 - Pf6N2E2_433 0.73 +0.2
501,122 - Pf6N2E2_433 0.73 +0.2
501,122 - Pf6N2E2_433 0.73 +0.1
501,122 - Pf6N2E2_433 0.73 -0.3

Or see this region's nucleotide sequence