Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1810 and Pf6N2E2_1811 are separated by 9 nucleotides Pf6N2E2_1811 and Pf6N2E2_1812 are separated by 31 nucleotides Pf6N2E2_1812 and Pf6N2E2_1813 overlap by 4 nucleotides Pf6N2E2_1813 and Pf6N2E2_1814 overlap by 4 nucleotides
Pf6N2E2_1810 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,142,347 to 2,143,264
_1810
Pf6N2E2_1811 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 2,143,274 to 2,144,176
_1811
Pf6N2E2_1812 - peptide ABC transporter, ATP-binding protein, at 2,144,208 to 2,145,224
_1812
Pf6N2E2_1813 - ABC-type oligopeptide transport system, ATPase component, at 2,145,221 to 2,146,210
_1813
Pf6N2E2_1814 - Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases, at 2,146,207 to 2,147,631
_1814
Position (kb)
2144
2145
2146 Strain fitness (log2 ratio)
-1
0
1 at 2143.271 kb on + strand at 2143.272 kb on - strand at 2143.272 kb on - strand at 2143.307 kb on + strand at 2143.436 kb on + strand, within Pf6N2E2_1811 at 2143.437 kb on - strand, within Pf6N2E2_1811 at 2143.508 kb on + strand, within Pf6N2E2_1811 at 2143.508 kb on + strand, within Pf6N2E2_1811 at 2143.508 kb on + strand, within Pf6N2E2_1811 at 2143.672 kb on + strand, within Pf6N2E2_1811 at 2143.680 kb on - strand, within Pf6N2E2_1811 at 2143.848 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.055 kb on - strand, within Pf6N2E2_1811 at 2144.190 kb on + strand at 2144.190 kb on + strand at 2144.190 kb on + strand at 2144.191 kb on - strand at 2144.191 kb on - strand at 2144.191 kb on - strand at 2144.194 kb on - strand at 2144.194 kb on - strand at 2144.194 kb on - strand at 2144.215 kb on + strand at 2144.216 kb on - strand at 2144.539 kb on - strand, within Pf6N2E2_1812 at 2144.539 kb on - strand, within Pf6N2E2_1812 at 2144.904 kb on + strand, within Pf6N2E2_1812 at 2144.905 kb on - strand, within Pf6N2E2_1812 at 2144.905 kb on - strand, within Pf6N2E2_1812 at 2144.967 kb on + strand, within Pf6N2E2_1812 at 2144.990 kb on + strand, within Pf6N2E2_1812 at 2145.403 kb on - strand, within Pf6N2E2_1813 at 2145.518 kb on + strand, within Pf6N2E2_1813 at 2145.518 kb on + strand, within Pf6N2E2_1813 at 2145.836 kb on + strand, within Pf6N2E2_1813 at 2145.837 kb on - strand, within Pf6N2E2_1813 at 2145.837 kb on - strand, within Pf6N2E2_1813 at 2145.837 kb on - strand, within Pf6N2E2_1813 at 2145.837 kb on - strand, within Pf6N2E2_1813 at 2145.841 kb on - strand, within Pf6N2E2_1813 at 2145.900 kb on - strand, within Pf6N2E2_1813 at 2145.940 kb on - strand, within Pf6N2E2_1813 at 2145.962 kb on + strand, within Pf6N2E2_1813 at 2145.963 kb on - strand, within Pf6N2E2_1813
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 2,143,271 + +0.6 2,143,272 - -0.8 2,143,272 - +0.9 2,143,307 + +0.3 2,143,436 + Pf6N2E2_1811 0.18 -0.7 2,143,437 - Pf6N2E2_1811 0.18 +0.7 2,143,508 + Pf6N2E2_1811 0.26 +0.0 2,143,508 + Pf6N2E2_1811 0.26 +0.8 2,143,508 + Pf6N2E2_1811 0.26 +0.6 2,143,672 + Pf6N2E2_1811 0.44 +0.8 2,143,680 - Pf6N2E2_1811 0.45 -0.5 2,143,848 - Pf6N2E2_1811 0.64 +0.5 2,144,055 - Pf6N2E2_1811 0.86 +1.7 2,144,055 - Pf6N2E2_1811 0.86 -0.2 2,144,055 - Pf6N2E2_1811 0.86 +0.7 2,144,055 - Pf6N2E2_1811 0.86 -0.1 2,144,055 - Pf6N2E2_1811 0.86 -0.3 2,144,055 - Pf6N2E2_1811 0.86 -0.2 2,144,190 + +0.6 2,144,190 + +0.3 2,144,190 + +0.5 2,144,191 - -0.0 2,144,191 - +1.0 2,144,191 - -0.4 2,144,194 - +0.7 2,144,194 - +0.2 2,144,194 - +0.4 2,144,215 + +0.0 2,144,216 - -0.3 2,144,539 - Pf6N2E2_1812 0.33 -0.3 2,144,539 - Pf6N2E2_1812 0.33 +0.1 2,144,904 + Pf6N2E2_1812 0.68 -1.3 2,144,905 - Pf6N2E2_1812 0.69 -1.1 2,144,905 - Pf6N2E2_1812 0.69 -0.3 2,144,967 + Pf6N2E2_1812 0.75 -0.2 2,144,990 + Pf6N2E2_1812 0.77 -0.2 2,145,403 - Pf6N2E2_1813 0.18 +0.5 2,145,518 + Pf6N2E2_1813 0.30 +0.5 2,145,518 + Pf6N2E2_1813 0.30 +0.2 2,145,836 + Pf6N2E2_1813 0.62 +1.5 2,145,837 - Pf6N2E2_1813 0.62 +0.3 2,145,837 - Pf6N2E2_1813 0.62 -0.3 2,145,837 - Pf6N2E2_1813 0.62 +0.8 2,145,837 - Pf6N2E2_1813 0.62 -0.1 2,145,841 - Pf6N2E2_1813 0.63 +0.6 2,145,900 - Pf6N2E2_1813 0.69 +1.3 2,145,940 - Pf6N2E2_1813 0.73 -0.2 2,145,962 + Pf6N2E2_1813 0.75 +0.1 2,145,963 - Pf6N2E2_1813 0.75 -0.2
Or see this region's nucleotide sequence