Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1375 and Pf6N2E2_1376 are separated by 194 nucleotides Pf6N2E2_1376 and Pf6N2E2_1377 are separated by 116 nucleotides
Pf6N2E2_1375 - Multiple polyol-specific dehydrogenase (EC 1.1.1.-), at 1,612,232 to 1,613,005
_1375
Pf6N2E2_1376 - probable transmembrane protein, at 1,613,200 to 1,614,051
_1376
Pf6N2E2_1377 - Malate:quinone oxidoreductase (EC 1.1.5.4), at 1,614,168 to 1,615,811
_1377
Position (kb)
1613
1614
1615 Strain fitness (log2 ratio)
-1
0
1
2 at 1612.202 kb on + strand at 1612.202 kb on + strand at 1612.205 kb on + strand at 1612.205 kb on + strand at 1612.205 kb on + strand at 1612.205 kb on + strand at 1612.206 kb on - strand at 1612.206 kb on - strand at 1612.206 kb on - strand at 1612.209 kb on + strand at 1612.210 kb on - strand at 1612.307 kb on + strand at 1612.308 kb on - strand at 1612.541 kb on + strand, within Pf6N2E2_1375 at 1612.688 kb on + strand, within Pf6N2E2_1375 at 1612.955 kb on + strand at 1612.956 kb on - strand at 1612.973 kb on + strand at 1612.973 kb on + strand at 1612.973 kb on + strand at 1612.973 kb on + strand at 1612.973 kb on + strand at 1612.973 kb on + strand at 1612.973 kb on + strand at 1612.973 kb on + strand at 1612.974 kb on - strand at 1612.974 kb on - strand at 1613.007 kb on - strand at 1613.019 kb on + strand at 1613.071 kb on + strand at 1613.136 kb on + strand at 1613.197 kb on + strand at 1613.197 kb on + strand at 1613.252 kb on + strand at 1613.252 kb on + strand at 1613.531 kb on + strand, within Pf6N2E2_1376 at 1613.531 kb on + strand, within Pf6N2E2_1376 at 1613.532 kb on - strand, within Pf6N2E2_1376 at 1613.532 kb on - strand, within Pf6N2E2_1376 at 1613.535 kb on - strand, within Pf6N2E2_1376 at 1613.535 kb on - strand, within Pf6N2E2_1376 at 1613.590 kb on - strand, within Pf6N2E2_1376 at 1613.854 kb on + strand, within Pf6N2E2_1376 at 1614.023 kb on - strand at 1614.023 kb on - strand at 1614.145 kb on + strand at 1614.146 kb on - strand at 1614.289 kb on + strand at 1614.611 kb on - strand, within Pf6N2E2_1377 at 1614.611 kb on - strand, within Pf6N2E2_1377 at 1614.611 kb on - strand, within Pf6N2E2_1377 at 1614.668 kb on - strand, within Pf6N2E2_1377 at 1614.752 kb on - strand, within Pf6N2E2_1377
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 1,612,202 + +0.1 1,612,202 + +1.3 1,612,205 + -0.9 1,612,205 + +0.1 1,612,205 + +0.1 1,612,205 + -0.2 1,612,206 - +0.5 1,612,206 - -0.7 1,612,206 - -0.3 1,612,209 + +0.5 1,612,210 - +0.4 1,612,307 + -0.3 1,612,308 - +0.4 1,612,541 + Pf6N2E2_1375 0.40 +0.2 1,612,688 + Pf6N2E2_1375 0.59 +0.6 1,612,955 + +1.4 1,612,956 - -0.2 1,612,973 + +0.4 1,612,973 + +0.5 1,612,973 + +1.0 1,612,973 + -0.8 1,612,973 + +0.4 1,612,973 + +0.1 1,612,973 + -1.4 1,612,973 + -0.4 1,612,974 - +0.3 1,612,974 - +0.9 1,613,007 - +1.6 1,613,019 + -0.1 1,613,071 + +0.4 1,613,136 + -0.3 1,613,197 + -0.3 1,613,197 + +0.2 1,613,252 + -0.3 1,613,252 + +0.2 1,613,531 + Pf6N2E2_1376 0.39 -1.5 1,613,531 + Pf6N2E2_1376 0.39 +0.1 1,613,532 - Pf6N2E2_1376 0.39 -1.2 1,613,532 - Pf6N2E2_1376 0.39 -1.1 1,613,535 - Pf6N2E2_1376 0.39 -0.9 1,613,535 - Pf6N2E2_1376 0.39 -0.3 1,613,590 - Pf6N2E2_1376 0.46 -0.5 1,613,854 + Pf6N2E2_1376 0.77 +0.3 1,614,023 - +0.4 1,614,023 - -0.1 1,614,145 + +2.1 1,614,146 - -0.5 1,614,289 + -0.8 1,614,611 - Pf6N2E2_1377 0.27 -0.6 1,614,611 - Pf6N2E2_1377 0.27 -0.0 1,614,611 - Pf6N2E2_1377 0.27 -0.8 1,614,668 - Pf6N2E2_1377 0.30 +0.0 1,614,752 - Pf6N2E2_1377 0.36 -0.4
Or see this region's nucleotide sequence