Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1090 and Pf6N2E2_1091 are separated by 193 nucleotides Pf6N2E2_1091 and Pf6N2E2_1092 overlap by 4 nucleotides
Pf6N2E2_1090 - Transcriptional regulator for ferulate or vanillate catabolism, at 1,277,735 to 1,278,448
_1090
Pf6N2E2_1091 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 1,278,642 to 1,279,592
_1091
Pf6N2E2_1092 - Probable vanillate O-demethylase oxygenase subunit oxidoreductase protein (EC 1.14.13.-), at 1,279,589 to 1,280,674
_1092
Position (kb)
1278
1279
1280 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1277.700 kb on + strand at 1277.700 kb on + strand at 1277.701 kb on - strand at 1277.701 kb on - strand at 1277.703 kb on + strand at 1277.703 kb on + strand at 1277.703 kb on + strand at 1277.704 kb on - strand at 1277.740 kb on + strand at 1277.741 kb on - strand at 1277.823 kb on + strand, within Pf6N2E2_1090 at 1278.092 kb on + strand, within Pf6N2E2_1090 at 1278.092 kb on + strand, within Pf6N2E2_1090 at 1278.093 kb on - strand, within Pf6N2E2_1090 at 1278.281 kb on + strand, within Pf6N2E2_1090 at 1278.281 kb on + strand, within Pf6N2E2_1090 at 1278.281 kb on + strand, within Pf6N2E2_1090 at 1278.282 kb on - strand, within Pf6N2E2_1090 at 1278.289 kb on + strand, within Pf6N2E2_1090 at 1278.289 kb on + strand, within Pf6N2E2_1090 at 1278.289 kb on + strand, within Pf6N2E2_1090 at 1278.289 kb on + strand, within Pf6N2E2_1090 at 1278.468 kb on + strand at 1278.468 kb on + strand at 1278.468 kb on + strand at 1278.468 kb on + strand at 1278.469 kb on - strand at 1278.537 kb on + strand at 1278.537 kb on + strand at 1278.538 kb on - strand at 1278.538 kb on - strand at 1278.550 kb on + strand at 1278.751 kb on - strand, within Pf6N2E2_1091 at 1278.751 kb on - strand, within Pf6N2E2_1091 at 1278.944 kb on - strand, within Pf6N2E2_1091 at 1278.944 kb on - strand, within Pf6N2E2_1091 at 1279.031 kb on - strand, within Pf6N2E2_1091 at 1279.183 kb on + strand, within Pf6N2E2_1091 at 1279.725 kb on - strand, within Pf6N2E2_1092 at 1279.872 kb on + strand, within Pf6N2E2_1092 at 1279.872 kb on + strand, within Pf6N2E2_1092 at 1280.199 kb on + strand, within Pf6N2E2_1092 at 1280.199 kb on + strand, within Pf6N2E2_1092 at 1280.200 kb on - strand, within Pf6N2E2_1092
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 1,277,700 + -0.3 1,277,700 + +0.3 1,277,701 - -0.5 1,277,701 - -0.9 1,277,703 + +0.2 1,277,703 + -0.3 1,277,703 + -0.8 1,277,704 - +0.3 1,277,740 + -0.3 1,277,741 - -0.6 1,277,823 + Pf6N2E2_1090 0.12 -0.1 1,278,092 + Pf6N2E2_1090 0.50 -0.2 1,278,092 + Pf6N2E2_1090 0.50 +1.1 1,278,093 - Pf6N2E2_1090 0.50 -0.1 1,278,281 + Pf6N2E2_1090 0.76 +1.0 1,278,281 + Pf6N2E2_1090 0.76 +0.8 1,278,281 + Pf6N2E2_1090 0.76 -0.2 1,278,282 - Pf6N2E2_1090 0.77 +1.0 1,278,289 + Pf6N2E2_1090 0.78 +0.3 1,278,289 + Pf6N2E2_1090 0.78 -0.7 1,278,289 + Pf6N2E2_1090 0.78 -0.4 1,278,289 + Pf6N2E2_1090 0.78 +0.3 1,278,468 + -0.0 1,278,468 + +1.0 1,278,468 + -0.2 1,278,468 + -0.8 1,278,469 - +0.2 1,278,537 + -1.9 1,278,537 + +0.0 1,278,538 - +0.1 1,278,538 - +0.2 1,278,550 + +0.1 1,278,751 - Pf6N2E2_1091 0.11 +1.3 1,278,751 - Pf6N2E2_1091 0.11 -0.1 1,278,944 - Pf6N2E2_1091 0.32 -0.8 1,278,944 - Pf6N2E2_1091 0.32 -0.4 1,279,031 - Pf6N2E2_1091 0.41 -0.5 1,279,183 + Pf6N2E2_1091 0.57 -2.5 1,279,725 - Pf6N2E2_1092 0.13 -0.0 1,279,872 + Pf6N2E2_1092 0.26 -0.4 1,279,872 + Pf6N2E2_1092 0.26 +0.4 1,280,199 + Pf6N2E2_1092 0.56 +2.2 1,280,199 + Pf6N2E2_1092 0.56 -0.9 1,280,200 - Pf6N2E2_1092 0.56 +1.0
Or see this region's nucleotide sequence