Experiment: nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1039 and Pf6N2E2_1040 are separated by 328 nucleotides Pf6N2E2_1040 and Pf6N2E2_1041 overlap by 4 nucleotides
Pf6N2E2_1039 - Gluconolactonase, at 1,212,812 to 1,213,738
_1039
Pf6N2E2_1040 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 1,214,067 to 1,215,008
_1040
Pf6N2E2_1041 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 1,215,005 to 1,216,204
_1041
Position (kb)
1214
1215
1216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1213.261 kb on - strand, within Pf6N2E2_1039 at 1213.266 kb on + strand, within Pf6N2E2_1039 at 1213.317 kb on + strand, within Pf6N2E2_1039 at 1213.318 kb on - strand, within Pf6N2E2_1039 at 1213.318 kb on - strand, within Pf6N2E2_1039 at 1213.500 kb on + strand, within Pf6N2E2_1039 at 1213.500 kb on + strand, within Pf6N2E2_1039 at 1213.572 kb on + strand, within Pf6N2E2_1039 at 1213.572 kb on + strand, within Pf6N2E2_1039 at 1213.729 kb on - strand at 1213.769 kb on + strand at 1213.770 kb on - strand at 1213.789 kb on + strand at 1213.789 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.951 kb on + strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1213.952 kb on - strand at 1214.075 kb on + strand at 1214.076 kb on - strand at 1214.076 kb on - strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.087 kb on - strand at 1214.087 kb on - strand at 1214.087 kb on - strand at 1214.090 kb on + strand at 1214.090 kb on + strand at 1214.091 kb on - strand at 1214.091 kb on - strand at 1214.091 kb on - strand at 1214.366 kb on + strand, within Pf6N2E2_1040 at 1214.513 kb on - strand, within Pf6N2E2_1040 at 1214.787 kb on - strand, within Pf6N2E2_1040 at 1215.069 kb on + strand at 1215.160 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.410 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.728 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 200 mM remove 1,213,261 - Pf6N2E2_1039 0.48 +0.6 1,213,266 + Pf6N2E2_1039 0.49 +0.1 1,213,317 + Pf6N2E2_1039 0.54 -0.2 1,213,318 - Pf6N2E2_1039 0.55 +0.6 1,213,318 - Pf6N2E2_1039 0.55 -0.6 1,213,500 + Pf6N2E2_1039 0.74 -0.3 1,213,500 + Pf6N2E2_1039 0.74 -0.2 1,213,572 + Pf6N2E2_1039 0.82 +0.7 1,213,572 + Pf6N2E2_1039 0.82 -0.7 1,213,729 - -0.5 1,213,769 + -0.2 1,213,770 - -0.0 1,213,789 + +0.5 1,213,789 + -1.5 1,213,951 + +0.1 1,213,951 + -0.0 1,213,951 + -0.1 1,213,951 + +0.6 1,213,951 + +0.0 1,213,951 + +0.7 1,213,951 + -1.0 1,213,951 + -0.4 1,213,951 + +0.3 1,213,951 + +0.7 1,213,951 + +0.5 1,213,951 + +0.0 1,213,951 + +0.6 1,213,951 + -1.3 1,213,951 + -0.2 1,213,951 + +0.1 1,213,951 + +0.5 1,213,951 + +0.6 1,213,951 + +1.2 1,213,951 + +0.3 1,213,951 + +0.3 1,213,951 + +0.3 1,213,951 + -0.3 1,213,951 + -0.2 1,213,951 + +0.2 1,213,951 + +0.3 1,213,951 + +0.2 1,213,951 + +0.7 1,213,952 - +0.6 1,213,952 - +0.2 1,213,952 - +0.7 1,213,952 - +1.0 1,213,952 - +0.1 1,213,952 - +0.0 1,213,952 - +0.5 1,213,952 - +0.2 1,213,952 - +0.2 1,213,952 - -0.2 1,213,952 - +0.2 1,213,952 - -0.5 1,213,952 - -0.4 1,213,952 - -0.6 1,213,952 - -0.9 1,213,952 - -3.0 1,213,952 - +1.0 1,213,952 - +0.6 1,213,952 - -0.1 1,213,952 - +0.1 1,213,952 - -0.4 1,213,952 - -1.3 1,213,952 - -0.7 1,213,952 - -1.1 1,213,952 - +0.9 1,214,075 + -0.3 1,214,076 - +0.3 1,214,076 - +0.7 1,214,086 + -1.4 1,214,086 + +0.2 1,214,086 + +0.3 1,214,086 + -0.3 1,214,086 + -0.5 1,214,086 + -0.2 1,214,086 + +1.1 1,214,087 - +0.7 1,214,087 - +1.1 1,214,087 - -0.3 1,214,090 + -0.2 1,214,090 + -0.7 1,214,091 - +0.1 1,214,091 - +0.5 1,214,091 - +0.4 1,214,366 + Pf6N2E2_1040 0.32 -1.0 1,214,513 - Pf6N2E2_1040 0.47 -0.0 1,214,787 - Pf6N2E2_1040 0.76 -0.9 1,215,069 + -1.1 1,215,160 + Pf6N2E2_1041 0.13 -0.4 1,215,203 + Pf6N2E2_1041 0.17 +0.9 1,215,203 + Pf6N2E2_1041 0.17 -0.1 1,215,203 + Pf6N2E2_1041 0.17 +0.4 1,215,203 + Pf6N2E2_1041 0.17 +0.0 1,215,203 + Pf6N2E2_1041 0.17 -0.9 1,215,203 + Pf6N2E2_1041 0.17 +1.3 1,215,377 + Pf6N2E2_1041 0.31 -0.7 1,215,377 + Pf6N2E2_1041 0.31 -0.6 1,215,377 + Pf6N2E2_1041 0.31 +0.1 1,215,377 + Pf6N2E2_1041 0.31 +2.7 1,215,377 + Pf6N2E2_1041 0.31 +0.2 1,215,377 + Pf6N2E2_1041 0.31 +0.4 1,215,378 - Pf6N2E2_1041 0.31 +0.4 1,215,378 - Pf6N2E2_1041 0.31 -0.0 1,215,378 - Pf6N2E2_1041 0.31 +0.0 1,215,378 - Pf6N2E2_1041 0.31 +0.3 1,215,378 - Pf6N2E2_1041 0.31 -0.5 1,215,410 + Pf6N2E2_1041 0.34 +0.5 1,215,464 + Pf6N2E2_1041 0.38 +0.3 1,215,464 + Pf6N2E2_1041 0.38 -0.9 1,215,464 + Pf6N2E2_1041 0.38 +0.8 1,215,465 - Pf6N2E2_1041 0.38 -1.5 1,215,465 - Pf6N2E2_1041 0.38 +0.6 1,215,465 - Pf6N2E2_1041 0.38 -0.0 1,215,465 - Pf6N2E2_1041 0.38 -0.2 1,215,713 + Pf6N2E2_1041 0.59 -0.6 1,215,713 + Pf6N2E2_1041 0.59 +0.6 1,215,713 + Pf6N2E2_1041 0.59 +0.9 1,215,713 + Pf6N2E2_1041 0.59 -0.9 1,215,728 - -0.6
Or see this region's nucleotide sequence