Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08145 and MMJJ_RS08150 are separated by 62 nucleotides MMJJ_RS08150 and MMJJ_RS08155 are separated by 39 nucleotides
MMJJ_RS08145: MMJJ_RS08145 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 1,495,446 to 1,496,735
_RS08145
MMJJ_RS08150: MMJJ_RS08150 - M24 family metallopeptidase, at 1,496,798 to 1,497,817
_RS08150
MMJJ_RS08155: MMJJ_RS08155 - cobalt-precorrin-5B (C(1))-methyltransferase CbiD, at 1,497,857 to 1,498,954
_RS08155
Position (kb)
1496
1497
1498 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1496.449 kb on - strand at 1496.743 kb on + strand at 1496.755 kb on - strand at 1496.758 kb on - strand at 1496.759 kb on + strand at 1496.761 kb on + strand at 1496.763 kb on - strand at 1496.763 kb on - strand at 1496.771 kb on - strand at 1496.781 kb on + strand at 1496.798 kb on + strand at 1496.830 kb on + strand at 1496.861 kb on + strand at 1496.876 kb on + strand at 1496.877 kb on - strand at 1496.877 kb on - strand at 1496.878 kb on + strand at 1496.878 kb on + strand at 1496.898 kb on + strand at 1496.932 kb on - strand, within MMJJ_RS08150 at 1496.932 kb on - strand at 1496.933 kb on + strand, within MMJJ_RS08150 at 1496.941 kb on - strand, within MMJJ_RS08150 at 1496.942 kb on + strand, within MMJJ_RS08150 at 1496.952 kb on - strand, within MMJJ_RS08150 at 1496.952 kb on - strand, within MMJJ_RS08150 at 1496.953 kb on + strand, within MMJJ_RS08150 at 1496.958 kb on - strand, within MMJJ_RS08150 at 1496.991 kb on + strand, within MMJJ_RS08150 at 1497.037 kb on - strand, within MMJJ_RS08150 at 1497.038 kb on + strand, within MMJJ_RS08150 at 1497.038 kb on + strand, within MMJJ_RS08150 at 1497.111 kb on - strand, within MMJJ_RS08150 at 1497.169 kb on - strand, within MMJJ_RS08150 at 1497.217 kb on - strand, within MMJJ_RS08150 at 1497.218 kb on + strand, within MMJJ_RS08150 at 1497.218 kb on + strand, within MMJJ_RS08150 at 1497.218 kb on + strand, within MMJJ_RS08150 at 1497.281 kb on + strand, within MMJJ_RS08150 at 1497.298 kb on - strand, within MMJJ_RS08150 at 1497.298 kb on - strand, within MMJJ_RS08150 at 1497.299 kb on + strand, within MMJJ_RS08150 at 1497.301 kb on - strand, within MMJJ_RS08150 at 1497.301 kb on - strand, within MMJJ_RS08150 at 1497.375 kb on - strand, within MMJJ_RS08150 at 1497.378 kb on - strand, within MMJJ_RS08150 at 1497.413 kb on - strand, within MMJJ_RS08150 at 1497.414 kb on + strand, within MMJJ_RS08150 at 1497.414 kb on + strand, within MMJJ_RS08150 at 1497.433 kb on - strand, within MMJJ_RS08150 at 1497.434 kb on + strand, within MMJJ_RS08150 at 1497.436 kb on - strand, within MMJJ_RS08150 at 1497.447 kb on - strand, within MMJJ_RS08150 at 1497.577 kb on - strand, within MMJJ_RS08150 at 1497.577 kb on - strand, within MMJJ_RS08150 at 1497.578 kb on + strand, within MMJJ_RS08150 at 1497.578 kb on + strand, within MMJJ_RS08150 at 1497.604 kb on + strand, within MMJJ_RS08150 at 1497.604 kb on + strand, within MMJJ_RS08150 at 1497.680 kb on + strand, within MMJJ_RS08150 at 1497.681 kb on - strand, within MMJJ_RS08150 at 1497.685 kb on + strand, within MMJJ_RS08150 at 1497.722 kb on - strand at 1497.722 kb on - strand at 1497.724 kb on - strand at 1497.724 kb on - strand at 1497.759 kb on + strand at 1497.813 kb on + strand at 1497.816 kb on + strand at 1497.820 kb on - strand at 1497.821 kb on + strand at 1497.829 kb on + strand at 1497.833 kb on - strand at 1497.834 kb on + strand at 1497.839 kb on - strand at 1497.856 kb on - strand at 1497.874 kb on - strand at 1497.896 kb on - strand at 1497.902 kb on + strand at 1498.091 kb on + strand, within MMJJ_RS08155 at 1498.120 kb on - strand at 1498.276 kb on + strand, within MMJJ_RS08155 at 1498.276 kb on + strand, within MMJJ_RS08155 at 1498.436 kb on - strand at 1498.497 kb on - strand at 1498.513 kb on - strand, within MMJJ_RS08155 at 1498.589 kb on - strand, within MMJJ_RS08155 at 1498.719 kb on - strand, within MMJJ_RS08155 at 1498.731 kb on + strand, within MMJJ_RS08155 at 1498.740 kb on - strand, within MMJJ_RS08155 at 1498.756 kb on + strand at 1498.787 kb on + strand, within MMJJ_RS08155 at 1498.799 kb on + strand, within MMJJ_RS08155
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,496,449 - -0.0 1,496,743 + -3.0 1,496,755 - -2.4 1,496,758 - -3.3 1,496,759 + -2.2 1,496,761 + -1.5 1,496,763 - -3.5 1,496,763 - -2.6 1,496,771 - -4.1 1,496,781 + -1.6 1,496,798 + -1.8 1,496,830 + -1.9 1,496,861 + -2.0 1,496,876 + -2.7 1,496,877 - -2.7 1,496,877 - -2.8 1,496,878 + -1.8 1,496,878 + -2.4 1,496,898 + -3.6 1,496,932 - MMJJ_RS08150 0.13 -2.1 1,496,932 - +1.0 1,496,933 + MMJJ_RS08150 0.13 -4.2 1,496,941 - MMJJ_RS08150 0.14 +0.4 1,496,942 + MMJJ_RS08150 0.14 -2.6 1,496,952 - MMJJ_RS08150 0.15 -2.3 1,496,952 - MMJJ_RS08150 0.15 -3.2 1,496,953 + MMJJ_RS08150 0.15 -1.6 1,496,958 - MMJJ_RS08150 0.16 -2.5 1,496,991 + MMJJ_RS08150 0.19 -3.3 1,497,037 - MMJJ_RS08150 0.23 -1.3 1,497,038 + MMJJ_RS08150 0.24 -2.3 1,497,038 + MMJJ_RS08150 0.24 -1.5 1,497,111 - MMJJ_RS08150 0.31 -3.0 1,497,169 - MMJJ_RS08150 0.36 -3.2 1,497,217 - MMJJ_RS08150 0.41 -4.3 1,497,218 + MMJJ_RS08150 0.41 -3.8 1,497,218 + MMJJ_RS08150 0.41 -2.0 1,497,218 + MMJJ_RS08150 0.41 -2.0 1,497,281 + MMJJ_RS08150 0.47 -3.4 1,497,298 - MMJJ_RS08150 0.49 -3.6 1,497,298 - MMJJ_RS08150 0.49 -2.6 1,497,299 + MMJJ_RS08150 0.49 -1.0 1,497,301 - MMJJ_RS08150 0.49 -2.4 1,497,301 - MMJJ_RS08150 0.49 -3.3 1,497,375 - MMJJ_RS08150 0.57 -1.8 1,497,378 - MMJJ_RS08150 0.57 -1.0 1,497,413 - MMJJ_RS08150 0.60 -3.2 1,497,414 + MMJJ_RS08150 0.60 -3.7 1,497,414 + MMJJ_RS08150 0.60 -1.6 1,497,433 - MMJJ_RS08150 0.62 -2.6 1,497,434 + MMJJ_RS08150 0.62 -1.5 1,497,436 - MMJJ_RS08150 0.63 -3.1 1,497,447 - MMJJ_RS08150 0.64 -1.4 1,497,577 - MMJJ_RS08150 0.76 -2.8 1,497,577 - MMJJ_RS08150 0.76 -2.9 1,497,578 + MMJJ_RS08150 0.76 -2.4 1,497,578 + MMJJ_RS08150 0.76 -0.7 1,497,604 + MMJJ_RS08150 0.79 -2.9 1,497,604 + MMJJ_RS08150 0.79 -2.6 1,497,680 + MMJJ_RS08150 0.86 -2.6 1,497,681 - MMJJ_RS08150 0.87 -3.2 1,497,685 + MMJJ_RS08150 0.87 -3.4 1,497,722 - -2.4 1,497,722 - -1.3 1,497,724 - -2.5 1,497,724 - -1.8 1,497,759 + -3.2 1,497,813 + +0.2 1,497,816 + +2.2 1,497,820 - -0.6 1,497,821 + +1.1 1,497,829 + -0.8 1,497,833 - -0.7 1,497,834 + -1.4 1,497,839 - -1.3 1,497,856 - -1.6 1,497,874 - -2.6 1,497,896 - -1.0 1,497,902 + -1.0 1,498,091 + MMJJ_RS08155 0.21 -0.0 1,498,120 - -1.0 1,498,276 + MMJJ_RS08155 0.38 -1.6 1,498,276 + MMJJ_RS08155 0.38 -3.6 1,498,436 - -0.0 1,498,497 - -0.0 1,498,513 - MMJJ_RS08155 0.60 -0.0 1,498,589 - MMJJ_RS08155 0.67 -3.2 1,498,719 - MMJJ_RS08155 0.79 -1.6 1,498,731 + MMJJ_RS08155 0.80 -2.0 1,498,740 - MMJJ_RS08155 0.80 -3.6 1,498,756 + -2.0 1,498,787 + MMJJ_RS08155 0.85 -3.8 1,498,799 + MMJJ_RS08155 0.86 -2.6
Or see this region's nucleotide sequence