Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07170 and MMJJ_RS07175 are separated by 157 nucleotides MMJJ_RS07175 and MMJJ_RS07180 are separated by 9 nucleotides MMJJ_RS07180 and MMJJ_RS07185 are separated by 2 nucleotides
MMJJ_RS07170: MMJJ_RS07170 - pyridoxal phosphate-dependent aminotransferase, at 1,310,715 to 1,312,019
_RS07170
MMJJ_RS07175: MMJJ_RS07175 - DEAD/DEAH box helicase, at 1,312,177 to 1,314,444
_RS07175
MMJJ_RS07180: MMJJ_RS07180 - type I 3-dehydroquinate dehydratase, at 1,314,454 to 1,315,110
_RS07180
MMJJ_RS07185: MMJJ_RS07185 - DUF371 domain-containing protein, at 1,315,113 to 1,315,529
_RS07185
Position (kb)
1312
1313
1314
1315 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1311.241 kb on - strand, within MMJJ_RS07170 at 1311.352 kb on + strand, within MMJJ_RS07170 at 1311.366 kb on - strand, within MMJJ_RS07170 at 1311.367 kb on + strand, within MMJJ_RS07170 at 1311.367 kb on + strand, within MMJJ_RS07170 at 1311.519 kb on - strand, within MMJJ_RS07170 at 1311.595 kb on - strand, within MMJJ_RS07170 at 1311.618 kb on + strand, within MMJJ_RS07170 at 1311.621 kb on - strand, within MMJJ_RS07170 at 1311.622 kb on + strand, within MMJJ_RS07170 at 1311.622 kb on + strand, within MMJJ_RS07170 at 1311.669 kb on - strand, within MMJJ_RS07170 at 1311.669 kb on - strand, within MMJJ_RS07170 at 1311.704 kb on + strand at 1311.804 kb on - strand, within MMJJ_RS07170 at 1311.852 kb on - strand, within MMJJ_RS07170 at 1311.855 kb on - strand, within MMJJ_RS07170 at 1311.859 kb on - strand, within MMJJ_RS07170 at 1312.005 kb on + strand at 1312.017 kb on - strand at 1312.056 kb on - strand at 1312.056 kb on - strand at 1312.056 kb on - strand at 1312.060 kb on - strand at 1312.061 kb on + strand at 1312.061 kb on + strand at 1312.061 kb on + strand at 1312.067 kb on + strand at 1312.077 kb on - strand at 1312.078 kb on + strand at 1312.084 kb on + strand at 1312.089 kb on - strand at 1312.096 kb on - strand at 1312.096 kb on - strand at 1312.097 kb on + strand at 1312.101 kb on + strand at 1312.103 kb on - strand at 1312.103 kb on - strand at 1312.103 kb on - strand at 1312.104 kb on + strand at 1312.104 kb on + strand at 1312.104 kb on + strand at 1312.104 kb on + strand at 1312.107 kb on - strand at 1312.107 kb on - strand at 1312.108 kb on + strand at 1312.108 kb on + strand at 1312.108 kb on + strand at 1312.109 kb on - strand at 1312.109 kb on - strand at 1312.109 kb on - strand at 1312.110 kb on + strand at 1312.110 kb on + strand at 1312.110 kb on + strand at 1312.110 kb on + strand at 1312.110 kb on + strand at 1312.110 kb on + strand at 1312.111 kb on - strand at 1312.111 kb on - strand at 1312.111 kb on - strand at 1312.111 kb on - strand at 1312.111 kb on - strand at 1312.111 kb on - strand at 1312.112 kb on + strand at 1312.112 kb on + strand at 1312.112 kb on + strand at 1312.112 kb on + strand at 1312.116 kb on - strand at 1312.117 kb on + strand at 1312.117 kb on + strand at 1312.117 kb on + strand at 1312.117 kb on + strand at 1312.118 kb on - strand at 1312.118 kb on - strand at 1312.121 kb on - strand at 1312.121 kb on - strand at 1312.122 kb on + strand at 1312.125 kb on + strand at 1312.126 kb on - strand at 1312.126 kb on - strand at 1312.126 kb on - strand at 1312.126 kb on - strand at 1312.127 kb on + strand at 1312.132 kb on - strand at 1312.176 kb on + strand at 1312.184 kb on - strand at 1312.184 kb on - strand at 1312.184 kb on - strand at 1312.184 kb on - strand at 1312.185 kb on + strand at 1312.185 kb on + strand at 1312.245 kb on + strand at 1312.257 kb on + strand at 1312.266 kb on - strand at 1312.266 kb on - strand at 1312.296 kb on - strand at 1312.318 kb on + strand at 1312.507 kb on + strand, within MMJJ_RS07175 at 1312.508 kb on - strand, within MMJJ_RS07175 at 1312.540 kb on + strand, within MMJJ_RS07175 at 1312.545 kb on - strand, within MMJJ_RS07175 at 1312.577 kb on - strand, within MMJJ_RS07175 at 1312.618 kb on - strand, within MMJJ_RS07175 at 1312.695 kb on - strand, within MMJJ_RS07175 at 1312.695 kb on - strand, within MMJJ_RS07175 at 1312.695 kb on - strand, within MMJJ_RS07175 at 1312.696 kb on + strand, within MMJJ_RS07175 at 1312.774 kb on - strand, within MMJJ_RS07175 at 1312.774 kb on - strand, within MMJJ_RS07175 at 1312.775 kb on + strand, within MMJJ_RS07175 at 1312.775 kb on + strand, within MMJJ_RS07175 at 1312.838 kb on - strand, within MMJJ_RS07175 at 1312.905 kb on - strand, within MMJJ_RS07175 at 1312.906 kb on + strand, within MMJJ_RS07175 at 1312.906 kb on + strand, within MMJJ_RS07175 at 1312.906 kb on + strand, within MMJJ_RS07175 at 1312.914 kb on + strand, within MMJJ_RS07175 at 1312.968 kb on - strand, within MMJJ_RS07175 at 1312.969 kb on + strand, within MMJJ_RS07175 at 1312.970 kb on - strand, within MMJJ_RS07175 at 1312.971 kb on + strand, within MMJJ_RS07175 at 1313.012 kb on - strand, within MMJJ_RS07175 at 1313.012 kb on - strand, within MMJJ_RS07175 at 1313.065 kb on - strand, within MMJJ_RS07175 at 1313.066 kb on + strand, within MMJJ_RS07175 at 1313.113 kb on + strand, within MMJJ_RS07175 at 1313.165 kb on - strand, within MMJJ_RS07175 at 1313.165 kb on - strand, within MMJJ_RS07175 at 1313.165 kb on - strand, within MMJJ_RS07175 at 1313.165 kb on - strand, within MMJJ_RS07175 at 1313.165 kb on - strand, within MMJJ_RS07175 at 1313.165 kb on - strand, within MMJJ_RS07175 at 1313.165 kb on - strand, within MMJJ_RS07175 at 1313.166 kb on + strand, within MMJJ_RS07175 at 1313.166 kb on + strand, within MMJJ_RS07175 at 1313.166 kb on + strand, within MMJJ_RS07175 at 1313.166 kb on + strand, within MMJJ_RS07175 at 1313.232 kb on + strand, within MMJJ_RS07175 at 1313.236 kb on + strand, within MMJJ_RS07175 at 1313.249 kb on + strand, within MMJJ_RS07175 at 1313.256 kb on - strand, within MMJJ_RS07175 at 1313.257 kb on + strand, within MMJJ_RS07175 at 1313.306 kb on - strand, within MMJJ_RS07175 at 1313.436 kb on - strand, within MMJJ_RS07175 at 1313.452 kb on - strand, within MMJJ_RS07175 at 1313.452 kb on - strand, within MMJJ_RS07175 at 1313.452 kb on - strand at 1313.459 kb on - strand, within MMJJ_RS07175 at 1313.460 kb on + strand, within MMJJ_RS07175 at 1313.465 kb on + strand, within MMJJ_RS07175 at 1313.538 kb on + strand, within MMJJ_RS07175 at 1313.546 kb on - strand, within MMJJ_RS07175 at 1313.622 kb on + strand, within MMJJ_RS07175 at 1313.637 kb on - strand, within MMJJ_RS07175 at 1313.638 kb on + strand, within MMJJ_RS07175 at 1313.642 kb on - strand, within MMJJ_RS07175 at 1313.642 kb on - strand, within MMJJ_RS07175 at 1313.643 kb on + strand, within MMJJ_RS07175 at 1313.663 kb on - strand, within MMJJ_RS07175 at 1313.709 kb on - strand, within MMJJ_RS07175 at 1313.710 kb on + strand, within MMJJ_RS07175 at 1313.710 kb on + strand, within MMJJ_RS07175 at 1313.713 kb on + strand, within MMJJ_RS07175 at 1313.722 kb on - strand, within MMJJ_RS07175 at 1313.722 kb on - strand, within MMJJ_RS07175 at 1313.723 kb on + strand, within MMJJ_RS07175 at 1313.732 kb on - strand, within MMJJ_RS07175 at 1313.733 kb on + strand, within MMJJ_RS07175 at 1313.744 kb on - strand, within MMJJ_RS07175 at 1313.744 kb on - strand, within MMJJ_RS07175 at 1313.744 kb on - strand, within MMJJ_RS07175 at 1313.745 kb on + strand, within MMJJ_RS07175 at 1313.745 kb on + strand, within MMJJ_RS07175 at 1313.752 kb on + strand, within MMJJ_RS07175 at 1313.763 kb on - strand, within MMJJ_RS07175 at 1313.768 kb on - strand, within MMJJ_RS07175 at 1313.768 kb on - strand, within MMJJ_RS07175 at 1313.769 kb on + strand, within MMJJ_RS07175 at 1313.769 kb on + strand, within MMJJ_RS07175 at 1313.805 kb on - strand, within MMJJ_RS07175 at 1313.806 kb on + strand, within MMJJ_RS07175 at 1313.834 kb on - strand, within MMJJ_RS07175 at 1313.835 kb on + strand, within MMJJ_RS07175 at 1313.835 kb on + strand, within MMJJ_RS07175 at 1313.837 kb on - strand, within MMJJ_RS07175 at 1313.859 kb on - strand, within MMJJ_RS07175 at 1313.869 kb on - strand, within MMJJ_RS07175 at 1313.923 kb on + strand, within MMJJ_RS07175 at 1313.928 kb on + strand, within MMJJ_RS07175 at 1314.000 kb on - strand, within MMJJ_RS07175 at 1314.008 kb on - strand, within MMJJ_RS07175 at 1314.017 kb on - strand, within MMJJ_RS07175 at 1314.066 kb on - strand, within MMJJ_RS07175 at 1314.125 kb on - strand, within MMJJ_RS07175 at 1314.132 kb on + strand, within MMJJ_RS07175 at 1314.156 kb on - strand, within MMJJ_RS07175 at 1314.156 kb on - strand, within MMJJ_RS07175 at 1314.157 kb on + strand, within MMJJ_RS07175 at 1314.216 kb on + strand, within MMJJ_RS07175 at 1314.216 kb on + strand, within MMJJ_RS07175 at 1314.219 kb on - strand at 1314.219 kb on - strand at 1314.220 kb on + strand at 1314.220 kb on + strand at 1314.220 kb on + strand at 1314.221 kb on - strand at 1314.249 kb on + strand at 1314.273 kb on + strand at 1314.328 kb on + strand at 1314.344 kb on - strand at 1314.345 kb on + strand at 1314.345 kb on + strand at 1314.349 kb on + strand at 1314.403 kb on + strand at 1314.452 kb on + strand at 1315.103 kb on - strand at 1315.108 kb on - strand at 1315.109 kb on + strand at 1315.224 kb on + strand, within MMJJ_RS07185 at 1315.391 kb on - strand, within MMJJ_RS07185 at 1315.392 kb on + strand, within MMJJ_RS07185 at 1315.392 kb on + strand, within MMJJ_RS07185
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,311,241 - MMJJ_RS07170 0.40 -0.8 1,311,352 + MMJJ_RS07170 0.49 -1.4 1,311,366 - MMJJ_RS07170 0.50 -2.4 1,311,367 + MMJJ_RS07170 0.50 -1.4 1,311,367 + MMJJ_RS07170 0.50 -2.4 1,311,519 - MMJJ_RS07170 0.62 -0.8 1,311,595 - MMJJ_RS07170 0.67 -0.8 1,311,618 + MMJJ_RS07170 0.69 -2.4 1,311,621 - MMJJ_RS07170 0.69 -3.3 1,311,622 + MMJJ_RS07170 0.70 -2.1 1,311,622 + MMJJ_RS07170 0.70 -1.4 1,311,669 - MMJJ_RS07170 0.73 +0.2 1,311,669 - MMJJ_RS07170 0.73 -1.4 1,311,704 + +0.2 1,311,804 - MMJJ_RS07170 0.83 -0.8 1,311,852 - MMJJ_RS07170 0.87 -0.8 1,311,855 - MMJJ_RS07170 0.87 -1.4 1,311,859 - MMJJ_RS07170 0.88 -2.1 1,312,005 + +0.9 1,312,017 - +0.6 1,312,056 - -0.8 1,312,056 - +1.1 1,312,056 - +0.5 1,312,060 - -1.4 1,312,061 + -0.7 1,312,061 + +1.1 1,312,061 + -1.7 1,312,067 + +0.7 1,312,077 - -0.4 1,312,078 + -0.2 1,312,084 + -0.2 1,312,089 - +0.4 1,312,096 - +0.3 1,312,096 - -0.2 1,312,097 + -0.0 1,312,101 + -0.1 1,312,103 - -1.0 1,312,103 - +1.0 1,312,103 - +1.7 1,312,104 + +0.2 1,312,104 + +0.2 1,312,104 + -0.9 1,312,104 + -0.0 1,312,107 - +0.1 1,312,107 - -1.6 1,312,108 + +0.7 1,312,108 + +1.3 1,312,108 + +0.5 1,312,109 - -0.5 1,312,109 - +1.1 1,312,109 - +2.8 1,312,110 + +0.1 1,312,110 + -0.1 1,312,110 + -1.6 1,312,110 + -0.0 1,312,110 + +2.1 1,312,110 + +1.2 1,312,111 - -1.8 1,312,111 - +1.0 1,312,111 - +0.4 1,312,111 - +0.5 1,312,111 - +2.0 1,312,111 - +2.1 1,312,112 + +1.0 1,312,112 + -0.1 1,312,112 + +0.2 1,312,112 + +1.2 1,312,116 - +1.8 1,312,117 + +2.7 1,312,117 + -0.4 1,312,117 + -0.1 1,312,117 + +0.2 1,312,118 - +0.6 1,312,118 - -0.3 1,312,121 - +0.2 1,312,121 - +1.8 1,312,122 + -0.6 1,312,125 + +2.0 1,312,126 - -0.2 1,312,126 - +1.1 1,312,126 - +0.9 1,312,126 - -0.4 1,312,127 + -0.6 1,312,132 - +2.1 1,312,176 + +0.2 1,312,184 - +0.5 1,312,184 - +0.3 1,312,184 - -0.1 1,312,184 - +0.6 1,312,185 + +0.7 1,312,185 + +1.5 1,312,245 + +1.2 1,312,257 + -0.7 1,312,266 - +1.8 1,312,266 - -0.6 1,312,296 - +0.4 1,312,318 + -0.8 1,312,507 + MMJJ_RS07175 0.15 +0.6 1,312,508 - MMJJ_RS07175 0.15 -0.6 1,312,540 + MMJJ_RS07175 0.16 -1.4 1,312,545 - MMJJ_RS07175 0.16 +1.5 1,312,577 - MMJJ_RS07175 0.18 -0.6 1,312,618 - MMJJ_RS07175 0.19 +0.8 1,312,695 - MMJJ_RS07175 0.23 -1.5 1,312,695 - MMJJ_RS07175 0.23 +1.3 1,312,695 - MMJJ_RS07175 0.23 +0.1 1,312,696 + MMJJ_RS07175 0.23 +0.2 1,312,774 - MMJJ_RS07175 0.26 -0.0 1,312,774 - MMJJ_RS07175 0.26 +0.7 1,312,775 + MMJJ_RS07175 0.26 +0.2 1,312,775 + MMJJ_RS07175 0.26 +0.9 1,312,838 - MMJJ_RS07175 0.29 -0.0 1,312,905 - MMJJ_RS07175 0.32 +0.6 1,312,906 + MMJJ_RS07175 0.32 +0.7 1,312,906 + MMJJ_RS07175 0.32 +1.2 1,312,906 + MMJJ_RS07175 0.32 +0.0 1,312,914 + MMJJ_RS07175 0.32 +2.0 1,312,968 - MMJJ_RS07175 0.35 +1.9 1,312,969 + MMJJ_RS07175 0.35 -0.2 1,312,970 - MMJJ_RS07175 0.35 +1.8 1,312,971 + MMJJ_RS07175 0.35 +2.5 1,313,012 - MMJJ_RS07175 0.37 +0.7 1,313,012 - MMJJ_RS07175 0.37 -1.6 1,313,065 - MMJJ_RS07175 0.39 +0.7 1,313,066 + MMJJ_RS07175 0.39 +3.4 1,313,113 + MMJJ_RS07175 0.41 +1.8 1,313,165 - MMJJ_RS07175 0.44 +0.2 1,313,165 - MMJJ_RS07175 0.44 +0.8 1,313,165 - MMJJ_RS07175 0.44 +1.4 1,313,165 - MMJJ_RS07175 0.44 +0.9 1,313,165 - MMJJ_RS07175 0.44 +0.4 1,313,165 - MMJJ_RS07175 0.44 +2.8 1,313,165 - MMJJ_RS07175 0.44 +0.3 1,313,166 + MMJJ_RS07175 0.44 +0.9 1,313,166 + MMJJ_RS07175 0.44 +1.6 1,313,166 + MMJJ_RS07175 0.44 +0.2 1,313,166 + MMJJ_RS07175 0.44 +0.4 1,313,232 + MMJJ_RS07175 0.47 +1.2 1,313,236 + MMJJ_RS07175 0.47 +1.1 1,313,249 + MMJJ_RS07175 0.47 -0.4 1,313,256 - MMJJ_RS07175 0.48 +0.3 1,313,257 + MMJJ_RS07175 0.48 +0.3 1,313,306 - MMJJ_RS07175 0.50 +0.2 1,313,436 - MMJJ_RS07175 0.56 +1.0 1,313,452 - MMJJ_RS07175 0.56 +1.0 1,313,452 - MMJJ_RS07175 0.56 +0.6 1,313,452 - +1.2 1,313,459 - MMJJ_RS07175 0.57 +0.8 1,313,460 + MMJJ_RS07175 0.57 -0.4 1,313,465 + MMJJ_RS07175 0.57 +0.1 1,313,538 + MMJJ_RS07175 0.60 +0.5 1,313,546 - MMJJ_RS07175 0.60 +0.3 1,313,622 + MMJJ_RS07175 0.64 +2.2 1,313,637 - MMJJ_RS07175 0.64 -0.9 1,313,638 + MMJJ_RS07175 0.64 +0.1 1,313,642 - MMJJ_RS07175 0.65 +1.0 1,313,642 - MMJJ_RS07175 0.65 +0.7 1,313,643 + MMJJ_RS07175 0.65 +0.2 1,313,663 - MMJJ_RS07175 0.66 +0.6 1,313,709 - MMJJ_RS07175 0.68 +0.3 1,313,710 + MMJJ_RS07175 0.68 +1.0 1,313,710 + MMJJ_RS07175 0.68 +1.0 1,313,713 + MMJJ_RS07175 0.68 +0.4 1,313,722 - MMJJ_RS07175 0.68 +1.1 1,313,722 - MMJJ_RS07175 0.68 +0.2 1,313,723 + MMJJ_RS07175 0.68 +2.2 1,313,732 - MMJJ_RS07175 0.69 -0.2 1,313,733 + MMJJ_RS07175 0.69 +0.4 1,313,744 - MMJJ_RS07175 0.69 +2.1 1,313,744 - MMJJ_RS07175 0.69 +2.1 1,313,744 - MMJJ_RS07175 0.69 -0.7 1,313,745 + MMJJ_RS07175 0.69 +2.2 1,313,745 + MMJJ_RS07175 0.69 -0.3 1,313,752 + MMJJ_RS07175 0.69 +0.2 1,313,763 - MMJJ_RS07175 0.70 +0.5 1,313,768 - MMJJ_RS07175 0.70 -0.0 1,313,768 - MMJJ_RS07175 0.70 +0.6 1,313,769 + MMJJ_RS07175 0.70 +0.8 1,313,769 + MMJJ_RS07175 0.70 +0.2 1,313,805 - MMJJ_RS07175 0.72 +2.1 1,313,806 + MMJJ_RS07175 0.72 +1.7 1,313,834 - MMJJ_RS07175 0.73 -0.4 1,313,835 + MMJJ_RS07175 0.73 +0.1 1,313,835 + MMJJ_RS07175 0.73 -0.6 1,313,837 - MMJJ_RS07175 0.73 +0.4 1,313,859 - MMJJ_RS07175 0.74 +0.5 1,313,869 - MMJJ_RS07175 0.75 +0.9 1,313,923 + MMJJ_RS07175 0.77 +1.0 1,313,928 + MMJJ_RS07175 0.77 -0.1 1,314,000 - MMJJ_RS07175 0.80 +1.8 1,314,008 - MMJJ_RS07175 0.81 +0.6 1,314,017 - MMJJ_RS07175 0.81 +0.5 1,314,066 - MMJJ_RS07175 0.83 -0.5 1,314,125 - MMJJ_RS07175 0.86 +0.1 1,314,132 + MMJJ_RS07175 0.86 +0.4 1,314,156 - MMJJ_RS07175 0.87 +1.8 1,314,156 - MMJJ_RS07175 0.87 +2.6 1,314,157 + MMJJ_RS07175 0.87 +1.6 1,314,216 + MMJJ_RS07175 0.90 +0.6 1,314,216 + MMJJ_RS07175 0.90 +1.0 1,314,219 - +0.2 1,314,219 - +0.4 1,314,220 + +0.8 1,314,220 + +3.1 1,314,220 + -0.0 1,314,221 - +0.3 1,314,249 + +2.7 1,314,273 + +0.9 1,314,328 + +0.1 1,314,344 - +0.2 1,314,345 + -0.2 1,314,345 + +2.1 1,314,349 + +0.9 1,314,403 + +1.1 1,314,452 + +0.2 1,315,103 - +0.2 1,315,108 - +0.3 1,315,109 + -0.2 1,315,224 + MMJJ_RS07185 0.27 +0.4 1,315,391 - MMJJ_RS07185 0.67 +0.6 1,315,392 + MMJJ_RS07185 0.67 +0.5 1,315,392 + MMJJ_RS07185 0.67 -1.4
Or see this region's nucleotide sequence