Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06705 and MMJJ_RS09325 are separated by 31 nucleotides MMJJ_RS09325 and MMJJ_RS06710 are separated by 12 nucleotides MMJJ_RS06710 and MMJJ_RS06715 are separated by 47 nucleotides
MMJJ_RS06705: MMJJ_RS06705 - bifunctional NADP phosphatase/NAD kinase, at 1,236,733 to 1,238,433
_RS06705
MMJJ_RS09325: MMJJ_RS09325 - hypothetical protein, at 1,238,465 to 1,238,623
_RS09325
MMJJ_RS06710: MMJJ_RS06710 - TldD/PmbA family protein, at 1,238,636 to 1,240,012
_RS06710
MMJJ_RS06715: MMJJ_RS06715 - lactaldehyde dehydrogenase, at 1,240,060 to 1,241,457
_RS06715
Position (kb)
1238
1239
1240
1241 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1237.638 kb on + strand, within MMJJ_RS06705 at 1237.658 kb on - strand, within MMJJ_RS06705 at 1237.664 kb on + strand, within MMJJ_RS06705 at 1237.841 kb on - strand, within MMJJ_RS06705 at 1237.842 kb on + strand, within MMJJ_RS06705 at 1238.035 kb on + strand, within MMJJ_RS06705 at 1238.076 kb on - strand at 1238.094 kb on + strand, within MMJJ_RS06705 at 1238.149 kb on - strand, within MMJJ_RS06705 at 1238.296 kb on - strand at 1238.296 kb on - strand at 1238.377 kb on - strand at 1238.420 kb on - strand at 1238.421 kb on + strand at 1238.426 kb on - strand at 1238.426 kb on - strand at 1238.426 kb on - strand at 1238.431 kb on - strand at 1238.431 kb on - strand at 1238.492 kb on - strand, within MMJJ_RS09325 at 1238.499 kb on - strand, within MMJJ_RS09325 at 1238.500 kb on + strand, within MMJJ_RS09325 at 1238.510 kb on + strand, within MMJJ_RS09325 at 1238.517 kb on + strand, within MMJJ_RS09325 at 1238.522 kb on - strand, within MMJJ_RS09325 at 1238.531 kb on - strand, within MMJJ_RS09325 at 1238.531 kb on - strand, within MMJJ_RS09325 at 1238.535 kb on + strand, within MMJJ_RS09325 at 1238.550 kb on - strand, within MMJJ_RS09325 at 1238.550 kb on - strand, within MMJJ_RS09325 at 1238.551 kb on + strand, within MMJJ_RS09325 at 1238.561 kb on - strand, within MMJJ_RS09325 at 1238.561 kb on - strand, within MMJJ_RS09325 at 1238.562 kb on + strand, within MMJJ_RS09325 at 1238.562 kb on + strand, within MMJJ_RS09325 at 1238.566 kb on - strand, within MMJJ_RS09325 at 1238.567 kb on + strand, within MMJJ_RS09325 at 1238.598 kb on + strand, within MMJJ_RS09325 at 1238.638 kb on + strand at 1238.660 kb on + strand at 1238.742 kb on + strand at 1238.848 kb on - strand, within MMJJ_RS06710 at 1238.906 kb on - strand, within MMJJ_RS06710 at 1238.907 kb on + strand, within MMJJ_RS06710 at 1238.908 kb on - strand, within MMJJ_RS06710 at 1238.921 kb on + strand, within MMJJ_RS06710 at 1238.927 kb on - strand, within MMJJ_RS06710 at 1238.959 kb on - strand, within MMJJ_RS06710 at 1238.959 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.968 kb on - strand, within MMJJ_RS06710 at 1238.968 kb on - strand, within MMJJ_RS06710 at 1238.968 kb on - strand, within MMJJ_RS06710 at 1238.968 kb on - strand, within MMJJ_RS06710 at 1238.969 kb on + strand, within MMJJ_RS06710 at 1238.969 kb on + strand, within MMJJ_RS06710 at 1238.969 kb on + strand, within MMJJ_RS06710 at 1238.969 kb on + strand, within MMJJ_RS06710 at 1238.971 kb on - strand, within MMJJ_RS06710 at 1238.972 kb on + strand, within MMJJ_RS06710 at 1238.992 kb on - strand, within MMJJ_RS06710 at 1238.992 kb on - strand, within MMJJ_RS06710 at 1238.997 kb on + strand, within MMJJ_RS06710 at 1239.067 kb on + strand, within MMJJ_RS06710 at 1239.067 kb on + strand, within MMJJ_RS06710 at 1239.074 kb on - strand, within MMJJ_RS06710 at 1239.112 kb on - strand, within MMJJ_RS06710 at 1239.136 kb on + strand, within MMJJ_RS06710 at 1239.173 kb on - strand, within MMJJ_RS06710 at 1239.180 kb on + strand, within MMJJ_RS06710 at 1239.285 kb on + strand, within MMJJ_RS06710 at 1239.406 kb on - strand, within MMJJ_RS06710 at 1239.437 kb on + strand, within MMJJ_RS06710 at 1239.564 kb on - strand, within MMJJ_RS06710 at 1239.682 kb on - strand, within MMJJ_RS06710 at 1239.683 kb on + strand, within MMJJ_RS06710 at 1239.683 kb on + strand, within MMJJ_RS06710 at 1239.683 kb on + strand, within MMJJ_RS06710 at 1239.730 kb on - strand, within MMJJ_RS06710 at 1239.734 kb on - strand, within MMJJ_RS06710 at 1239.734 kb on - strand, within MMJJ_RS06710 at 1239.734 kb on - strand, within MMJJ_RS06710 at 1239.734 kb on - strand, within MMJJ_RS06710 at 1239.734 kb on - strand, within MMJJ_RS06710 at 1239.734 kb on - strand, within MMJJ_RS06710 at 1239.734 kb on - strand, within MMJJ_RS06710 at 1239.734 kb on - strand, within MMJJ_RS06710 at 1239.734 kb on - strand, within MMJJ_RS06710 at 1239.735 kb on + strand, within MMJJ_RS06710 at 1239.735 kb on + strand, within MMJJ_RS06710 at 1239.754 kb on - strand, within MMJJ_RS06710 at 1239.755 kb on + strand, within MMJJ_RS06710 at 1239.773 kb on - strand, within MMJJ_RS06710 at 1239.812 kb on + strand, within MMJJ_RS06710 at 1239.812 kb on + strand, within MMJJ_RS06710 at 1239.839 kb on - strand, within MMJJ_RS06710 at 1239.839 kb on - strand, within MMJJ_RS06710 at 1239.840 kb on + strand, within MMJJ_RS06710 at 1239.845 kb on + strand, within MMJJ_RS06710 at 1239.851 kb on - strand, within MMJJ_RS06710 at 1239.884 kb on - strand at 1239.907 kb on - strand at 1239.946 kb on + strand at 1239.986 kb on - strand at 1240.030 kb on + strand at 1240.034 kb on + strand at 1240.037 kb on + strand at 1240.041 kb on - strand at 1240.042 kb on + strand at 1240.042 kb on + strand at 1240.042 kb on + strand at 1240.061 kb on - strand at 1240.076 kb on + strand at 1240.087 kb on - strand at 1240.111 kb on + strand at 1240.112 kb on - strand at 1240.113 kb on + strand at 1240.194 kb on - strand at 1240.211 kb on - strand, within MMJJ_RS06715 at 1240.242 kb on - strand, within MMJJ_RS06715 at 1240.247 kb on + strand, within MMJJ_RS06715 at 1240.251 kb on - strand, within MMJJ_RS06715 at 1240.252 kb on + strand, within MMJJ_RS06715 at 1240.390 kb on + strand, within MMJJ_RS06715 at 1240.392 kb on - strand, within MMJJ_RS06715 at 1240.438 kb on - strand, within MMJJ_RS06715 at 1240.438 kb on - strand, within MMJJ_RS06715 at 1240.489 kb on - strand, within MMJJ_RS06715 at 1240.509 kb on - strand, within MMJJ_RS06715 at 1240.509 kb on - strand, within MMJJ_RS06715 at 1240.509 kb on - strand, within MMJJ_RS06715 at 1240.510 kb on + strand, within MMJJ_RS06715 at 1240.510 kb on + strand, within MMJJ_RS06715 at 1240.575 kb on - strand, within MMJJ_RS06715 at 1240.580 kb on - strand, within MMJJ_RS06715 at 1240.580 kb on - strand, within MMJJ_RS06715 at 1240.580 kb on - strand, within MMJJ_RS06715 at 1240.580 kb on - strand, within MMJJ_RS06715 at 1240.618 kb on - strand, within MMJJ_RS06715 at 1240.618 kb on - strand, within MMJJ_RS06715 at 1240.635 kb on - strand, within MMJJ_RS06715 at 1240.693 kb on + strand, within MMJJ_RS06715 at 1240.725 kb on - strand, within MMJJ_RS06715 at 1240.774 kb on - strand, within MMJJ_RS06715 at 1240.820 kb on + strand, within MMJJ_RS06715 at 1240.937 kb on - strand, within MMJJ_RS06715 at 1240.937 kb on - strand, within MMJJ_RS06715 at 1240.937 kb on - strand, within MMJJ_RS06715 at 1240.937 kb on - strand, within MMJJ_RS06715 at 1240.937 kb on - strand, within MMJJ_RS06715 at 1240.938 kb on + strand, within MMJJ_RS06715 at 1240.938 kb on + strand, within MMJJ_RS06715 at 1240.939 kb on - strand, within MMJJ_RS06715 at 1240.940 kb on + strand, within MMJJ_RS06715 at 1240.975 kb on - strand, within MMJJ_RS06715 at 1240.976 kb on + strand, within MMJJ_RS06715 at 1240.977 kb on - strand, within MMJJ_RS06715
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,237,638 + MMJJ_RS06705 0.53 -0.8 1,237,658 - MMJJ_RS06705 0.54 -0.8 1,237,664 + MMJJ_RS06705 0.55 +0.2 1,237,841 - MMJJ_RS06705 0.65 -2.6 1,237,842 + MMJJ_RS06705 0.65 +0.2 1,238,035 + MMJJ_RS06705 0.77 -1.8 1,238,076 - -1.7 1,238,094 + MMJJ_RS06705 0.80 -2.1 1,238,149 - MMJJ_RS06705 0.83 +0.3 1,238,296 - -2.5 1,238,296 - -1.3 1,238,377 - -1.7 1,238,420 - -0.4 1,238,421 + +2.9 1,238,426 - -2.4 1,238,426 - -0.3 1,238,426 - -1.6 1,238,431 - +2.5 1,238,431 - -0.4 1,238,492 - MMJJ_RS09325 0.17 +2.1 1,238,499 - MMJJ_RS09325 0.21 -1.3 1,238,500 + MMJJ_RS09325 0.22 +0.1 1,238,510 + MMJJ_RS09325 0.28 -0.0 1,238,517 + MMJJ_RS09325 0.33 +0.3 1,238,522 - MMJJ_RS09325 0.36 -0.2 1,238,531 - MMJJ_RS09325 0.42 +2.3 1,238,531 - MMJJ_RS09325 0.42 +1.3 1,238,535 + MMJJ_RS09325 0.44 +0.6 1,238,550 - MMJJ_RS09325 0.53 -0.0 1,238,550 - MMJJ_RS09325 0.53 +1.2 1,238,551 + MMJJ_RS09325 0.54 -0.0 1,238,561 - MMJJ_RS09325 0.60 +1.0 1,238,561 - MMJJ_RS09325 0.60 +0.3 1,238,562 + MMJJ_RS09325 0.61 -0.7 1,238,562 + MMJJ_RS09325 0.61 +0.3 1,238,566 - MMJJ_RS09325 0.64 -0.8 1,238,567 + MMJJ_RS09325 0.64 +2.1 1,238,598 + MMJJ_RS09325 0.84 -0.4 1,238,638 + +0.7 1,238,660 + +0.9 1,238,742 + +0.4 1,238,848 - MMJJ_RS06710 0.15 +0.3 1,238,906 - MMJJ_RS06710 0.20 +0.3 1,238,907 + MMJJ_RS06710 0.20 -1.1 1,238,908 - MMJJ_RS06710 0.20 +0.4 1,238,921 + MMJJ_RS06710 0.21 +0.1 1,238,927 - MMJJ_RS06710 0.21 +2.2 1,238,959 - MMJJ_RS06710 0.23 +0.5 1,238,959 - MMJJ_RS06710 0.23 +0.8 1,238,964 - MMJJ_RS06710 0.24 +1.1 1,238,964 - MMJJ_RS06710 0.24 +1.0 1,238,964 - MMJJ_RS06710 0.24 -0.0 1,238,964 - MMJJ_RS06710 0.24 +0.1 1,238,964 - MMJJ_RS06710 0.24 -0.5 1,238,964 - MMJJ_RS06710 0.24 +0.2 1,238,964 - MMJJ_RS06710 0.24 +2.9 1,238,964 - MMJJ_RS06710 0.24 -0.6 1,238,965 + MMJJ_RS06710 0.24 -0.3 1,238,965 + MMJJ_RS06710 0.24 +0.4 1,238,965 + MMJJ_RS06710 0.24 -0.2 1,238,965 + MMJJ_RS06710 0.24 -0.2 1,238,965 + MMJJ_RS06710 0.24 +0.7 1,238,965 + MMJJ_RS06710 0.24 +0.3 1,238,965 + MMJJ_RS06710 0.24 +0.4 1,238,968 - MMJJ_RS06710 0.24 +2.1 1,238,968 - MMJJ_RS06710 0.24 +1.5 1,238,968 - MMJJ_RS06710 0.24 +0.6 1,238,968 - MMJJ_RS06710 0.24 +0.0 1,238,969 + MMJJ_RS06710 0.24 -1.6 1,238,969 + MMJJ_RS06710 0.24 +1.1 1,238,969 + MMJJ_RS06710 0.24 -0.7 1,238,969 + MMJJ_RS06710 0.24 -0.2 1,238,971 - MMJJ_RS06710 0.24 +0.5 1,238,972 + MMJJ_RS06710 0.24 +0.7 1,238,992 - MMJJ_RS06710 0.26 +0.2 1,238,992 - MMJJ_RS06710 0.26 +1.1 1,238,997 + MMJJ_RS06710 0.26 -2.3 1,239,067 + MMJJ_RS06710 0.31 +0.4 1,239,067 + MMJJ_RS06710 0.31 +1.6 1,239,074 - MMJJ_RS06710 0.32 +2.6 1,239,112 - MMJJ_RS06710 0.35 -0.1 1,239,136 + MMJJ_RS06710 0.36 +0.4 1,239,173 - MMJJ_RS06710 0.39 +0.5 1,239,180 + MMJJ_RS06710 0.40 +0.2 1,239,285 + MMJJ_RS06710 0.47 +0.3 1,239,406 - MMJJ_RS06710 0.56 -0.9 1,239,437 + MMJJ_RS06710 0.58 +1.6 1,239,564 - MMJJ_RS06710 0.67 +0.0 1,239,682 - MMJJ_RS06710 0.76 +0.7 1,239,683 + MMJJ_RS06710 0.76 -0.0 1,239,683 + MMJJ_RS06710 0.76 +2.0 1,239,683 + MMJJ_RS06710 0.76 -1.2 1,239,730 - MMJJ_RS06710 0.79 +0.6 1,239,734 - MMJJ_RS06710 0.80 +1.4 1,239,734 - MMJJ_RS06710 0.80 +0.3 1,239,734 - MMJJ_RS06710 0.80 +0.0 1,239,734 - MMJJ_RS06710 0.80 +0.5 1,239,734 - MMJJ_RS06710 0.80 +2.3 1,239,734 - MMJJ_RS06710 0.80 -0.7 1,239,734 - MMJJ_RS06710 0.80 -0.7 1,239,734 - MMJJ_RS06710 0.80 +0.2 1,239,734 - MMJJ_RS06710 0.80 +1.2 1,239,735 + MMJJ_RS06710 0.80 -0.4 1,239,735 + MMJJ_RS06710 0.80 +0.3 1,239,754 - MMJJ_RS06710 0.81 +0.8 1,239,755 + MMJJ_RS06710 0.81 -0.0 1,239,773 - MMJJ_RS06710 0.83 +0.1 1,239,812 + MMJJ_RS06710 0.85 -0.8 1,239,812 + MMJJ_RS06710 0.85 +0.7 1,239,839 - MMJJ_RS06710 0.87 +2.2 1,239,839 - MMJJ_RS06710 0.87 -0.2 1,239,840 + MMJJ_RS06710 0.87 +1.1 1,239,845 + MMJJ_RS06710 0.88 +0.6 1,239,851 - MMJJ_RS06710 0.88 -0.4 1,239,884 - -0.4 1,239,907 - +0.0 1,239,946 + +0.1 1,239,986 - +0.9 1,240,030 + -1.2 1,240,034 + -0.4 1,240,037 + +0.6 1,240,041 - +0.3 1,240,042 + +0.3 1,240,042 + +1.5 1,240,042 + +0.3 1,240,061 - -1.4 1,240,076 + -0.0 1,240,087 - -4.2 1,240,111 + -0.7 1,240,112 - -1.8 1,240,113 + -0.9 1,240,194 - -2.3 1,240,211 - MMJJ_RS06715 0.11 -2.6 1,240,242 - MMJJ_RS06715 0.13 -1.7 1,240,247 + MMJJ_RS06715 0.13 -3.1 1,240,251 - MMJJ_RS06715 0.14 -1.7 1,240,252 + MMJJ_RS06715 0.14 -3.3 1,240,390 + MMJJ_RS06715 0.24 -2.2 1,240,392 - MMJJ_RS06715 0.24 -3.3 1,240,438 - MMJJ_RS06715 0.27 -1.9 1,240,438 - MMJJ_RS06715 0.27 -2.7 1,240,489 - MMJJ_RS06715 0.31 -2.0 1,240,509 - MMJJ_RS06715 0.32 -2.7 1,240,509 - MMJJ_RS06715 0.32 -1.9 1,240,509 - MMJJ_RS06715 0.32 -4.0 1,240,510 + MMJJ_RS06715 0.32 -3.6 1,240,510 + MMJJ_RS06715 0.32 -4.2 1,240,575 - MMJJ_RS06715 0.37 -0.8 1,240,580 - MMJJ_RS06715 0.37 -0.6 1,240,580 - MMJJ_RS06715 0.37 -2.1 1,240,580 - MMJJ_RS06715 0.37 -0.8 1,240,580 - MMJJ_RS06715 0.37 -0.9 1,240,618 - MMJJ_RS06715 0.40 -4.8 1,240,618 - MMJJ_RS06715 0.40 -2.2 1,240,635 - MMJJ_RS06715 0.41 -1.3 1,240,693 + MMJJ_RS06715 0.45 -0.0 1,240,725 - MMJJ_RS06715 0.48 -2.0 1,240,774 - MMJJ_RS06715 0.51 -1.5 1,240,820 + MMJJ_RS06715 0.54 -2.7 1,240,937 - MMJJ_RS06715 0.63 -2.4 1,240,937 - MMJJ_RS06715 0.63 -1.7 1,240,937 - MMJJ_RS06715 0.63 -0.9 1,240,937 - MMJJ_RS06715 0.63 -3.2 1,240,937 - MMJJ_RS06715 0.63 -2.2 1,240,938 + MMJJ_RS06715 0.63 -1.7 1,240,938 + MMJJ_RS06715 0.63 -3.0 1,240,939 - MMJJ_RS06715 0.63 -3.8 1,240,940 + MMJJ_RS06715 0.63 -2.2 1,240,975 - MMJJ_RS06715 0.65 -0.3 1,240,976 + MMJJ_RS06715 0.66 -3.0 1,240,977 - MMJJ_RS06715 0.66 -2.3
Or see this region's nucleotide sequence