Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06695 and MMJJ_RS06700 are separated by 2 nucleotides MMJJ_RS06700 and MMJJ_RS06705 are separated by 91 nucleotides MMJJ_RS06705 and MMJJ_RS09325 are separated by 31 nucleotides MMJJ_RS09325 and MMJJ_RS06710 are separated by 12 nucleotides
MMJJ_RS06695: MMJJ_RS06695 - amidohydrolase, at 1,234,603 to 1,235,871
_RS06695
MMJJ_RS06700: MMJJ_RS06700 - L-seryl-tRNA(Sec) kinase, at 1,235,874 to 1,236,641
_RS06700
MMJJ_RS06705: MMJJ_RS06705 - bifunctional NADP phosphatase/NAD kinase, at 1,236,733 to 1,238,433
_RS06705
MMJJ_RS09325: MMJJ_RS09325 - hypothetical protein, at 1,238,465 to 1,238,623
_RS09325
MMJJ_RS06710: MMJJ_RS06710 - TldD/PmbA family protein, at 1,238,636 to 1,240,012
_RS06710
Position (kb)
1236
1237
1238
1239 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1235.746 kb on + strand at 1235.769 kb on + strand at 1235.851 kb on - strand at 1235.852 kb on + strand at 1235.852 kb on + strand at 1235.896 kb on - strand at 1235.901 kb on - strand at 1236.086 kb on - strand, within MMJJ_RS06700 at 1236.104 kb on - strand, within MMJJ_RS06700 at 1236.107 kb on - strand, within MMJJ_RS06700 at 1236.107 kb on - strand, within MMJJ_RS06700 at 1236.108 kb on + strand, within MMJJ_RS06700 at 1236.204 kb on + strand, within MMJJ_RS06700 at 1236.211 kb on + strand, within MMJJ_RS06700 at 1236.223 kb on - strand, within MMJJ_RS06700 at 1236.253 kb on - strand, within MMJJ_RS06700 at 1236.254 kb on + strand, within MMJJ_RS06700 at 1236.339 kb on - strand at 1236.340 kb on + strand, within MMJJ_RS06700 at 1236.340 kb on + strand, within MMJJ_RS06700 at 1236.528 kb on - strand, within MMJJ_RS06700 at 1236.528 kb on - strand, within MMJJ_RS06700 at 1236.636 kb on - strand at 1236.636 kb on - strand at 1236.660 kb on + strand at 1236.671 kb on - strand at 1236.672 kb on + strand at 1236.693 kb on - strand at 1236.694 kb on + strand at 1236.694 kb on + strand at 1236.697 kb on - strand at 1236.697 kb on - strand at 1236.699 kb on - strand at 1236.702 kb on - strand at 1236.706 kb on - strand at 1236.709 kb on - strand at 1236.709 kb on - strand at 1236.709 kb on - strand at 1236.709 kb on - strand at 1236.710 kb on + strand at 1236.710 kb on + strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.713 kb on - strand at 1236.714 kb on + strand at 1236.714 kb on + strand at 1236.714 kb on + strand at 1236.717 kb on - strand at 1236.717 kb on - strand at 1236.720 kb on - strand at 1236.720 kb on - strand at 1236.756 kb on + strand at 1236.888 kb on + strand at 1237.003 kb on - strand at 1237.290 kb on - strand, within MMJJ_RS06705 at 1237.299 kb on + strand, within MMJJ_RS06705 at 1237.493 kb on - strand, within MMJJ_RS06705 at 1237.524 kb on - strand, within MMJJ_RS06705 at 1237.524 kb on - strand, within MMJJ_RS06705 at 1237.524 kb on - strand, within MMJJ_RS06705 at 1237.526 kb on - strand, within MMJJ_RS06705 at 1237.638 kb on + strand, within MMJJ_RS06705 at 1237.658 kb on - strand, within MMJJ_RS06705 at 1237.664 kb on + strand, within MMJJ_RS06705 at 1237.841 kb on - strand, within MMJJ_RS06705 at 1237.842 kb on + strand, within MMJJ_RS06705 at 1238.035 kb on + strand, within MMJJ_RS06705 at 1238.076 kb on - strand at 1238.094 kb on + strand, within MMJJ_RS06705 at 1238.149 kb on - strand, within MMJJ_RS06705 at 1238.296 kb on - strand at 1238.296 kb on - strand at 1238.377 kb on - strand at 1238.420 kb on - strand at 1238.421 kb on + strand at 1238.426 kb on - strand at 1238.426 kb on - strand at 1238.426 kb on - strand at 1238.431 kb on - strand at 1238.431 kb on - strand at 1238.492 kb on - strand, within MMJJ_RS09325 at 1238.499 kb on - strand, within MMJJ_RS09325 at 1238.500 kb on + strand, within MMJJ_RS09325 at 1238.510 kb on + strand, within MMJJ_RS09325 at 1238.517 kb on + strand, within MMJJ_RS09325 at 1238.522 kb on - strand, within MMJJ_RS09325 at 1238.531 kb on - strand, within MMJJ_RS09325 at 1238.531 kb on - strand, within MMJJ_RS09325 at 1238.535 kb on + strand, within MMJJ_RS09325 at 1238.550 kb on - strand, within MMJJ_RS09325 at 1238.550 kb on - strand, within MMJJ_RS09325 at 1238.551 kb on + strand, within MMJJ_RS09325 at 1238.561 kb on - strand, within MMJJ_RS09325 at 1238.561 kb on - strand, within MMJJ_RS09325 at 1238.562 kb on + strand, within MMJJ_RS09325 at 1238.562 kb on + strand, within MMJJ_RS09325 at 1238.566 kb on - strand, within MMJJ_RS09325 at 1238.567 kb on + strand, within MMJJ_RS09325 at 1238.598 kb on + strand, within MMJJ_RS09325 at 1238.638 kb on + strand at 1238.660 kb on + strand at 1238.742 kb on + strand at 1238.848 kb on - strand, within MMJJ_RS06710 at 1238.906 kb on - strand, within MMJJ_RS06710 at 1238.907 kb on + strand, within MMJJ_RS06710 at 1238.908 kb on - strand, within MMJJ_RS06710 at 1238.921 kb on + strand, within MMJJ_RS06710 at 1238.927 kb on - strand, within MMJJ_RS06710 at 1238.959 kb on - strand, within MMJJ_RS06710 at 1238.959 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.964 kb on - strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.965 kb on + strand, within MMJJ_RS06710 at 1238.968 kb on - strand, within MMJJ_RS06710 at 1238.968 kb on - strand, within MMJJ_RS06710 at 1238.968 kb on - strand, within MMJJ_RS06710 at 1238.968 kb on - strand, within MMJJ_RS06710 at 1238.969 kb on + strand, within MMJJ_RS06710 at 1238.969 kb on + strand, within MMJJ_RS06710 at 1238.969 kb on + strand, within MMJJ_RS06710 at 1238.969 kb on + strand, within MMJJ_RS06710 at 1238.971 kb on - strand, within MMJJ_RS06710 at 1238.972 kb on + strand, within MMJJ_RS06710 at 1238.992 kb on - strand, within MMJJ_RS06710 at 1238.992 kb on - strand, within MMJJ_RS06710 at 1238.997 kb on + strand, within MMJJ_RS06710 at 1239.067 kb on + strand, within MMJJ_RS06710 at 1239.067 kb on + strand, within MMJJ_RS06710 at 1239.074 kb on - strand, within MMJJ_RS06710 at 1239.112 kb on - strand, within MMJJ_RS06710 at 1239.136 kb on + strand, within MMJJ_RS06710 at 1239.173 kb on - strand, within MMJJ_RS06710 at 1239.180 kb on + strand, within MMJJ_RS06710 at 1239.285 kb on + strand, within MMJJ_RS06710 at 1239.406 kb on - strand, within MMJJ_RS06710
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,235,746 + +0.2 1,235,769 + -0.8 1,235,851 - -0.2 1,235,852 + +0.3 1,235,852 + -0.4 1,235,896 - -1.3 1,235,901 - -1.3 1,236,086 - MMJJ_RS06700 0.28 -3.2 1,236,104 - MMJJ_RS06700 0.30 -2.6 1,236,107 - MMJJ_RS06700 0.30 -2.6 1,236,107 - MMJJ_RS06700 0.30 -1.0 1,236,108 + MMJJ_RS06700 0.30 -1.1 1,236,204 + MMJJ_RS06700 0.43 -2.6 1,236,211 + MMJJ_RS06700 0.44 -2.6 1,236,223 - MMJJ_RS06700 0.45 -1.8 1,236,253 - MMJJ_RS06700 0.49 -0.8 1,236,254 + MMJJ_RS06700 0.49 -2.8 1,236,339 - +0.2 1,236,340 + MMJJ_RS06700 0.61 -1.8 1,236,340 + MMJJ_RS06700 0.61 -2.1 1,236,528 - MMJJ_RS06700 0.85 -1.3 1,236,528 - MMJJ_RS06700 0.85 -2.9 1,236,636 - -0.8 1,236,636 - -2.8 1,236,660 + -2.0 1,236,671 - -0.8 1,236,672 + -1.8 1,236,693 - -0.9 1,236,694 + -0.9 1,236,694 + +2.5 1,236,697 - -0.6 1,236,697 - +1.0 1,236,699 - +0.1 1,236,702 - -1.6 1,236,706 - -0.6 1,236,709 - -0.1 1,236,709 - -0.8 1,236,709 - -0.2 1,236,709 - +2.6 1,236,710 + -1.0 1,236,710 + -2.3 1,236,713 - -0.6 1,236,713 - -1.1 1,236,713 - -0.8 1,236,713 - +1.5 1,236,713 - -1.1 1,236,713 - -0.8 1,236,713 - -0.9 1,236,714 + +0.1 1,236,714 + -3.7 1,236,714 + +0.1 1,236,717 - -0.8 1,236,717 - -0.3 1,236,720 - +0.2 1,236,720 - -2.0 1,236,756 + -0.8 1,236,888 + -2.3 1,237,003 - -1.3 1,237,290 - MMJJ_RS06705 0.33 -2.6 1,237,299 + MMJJ_RS06705 0.33 -1.8 1,237,493 - MMJJ_RS06705 0.45 -1.3 1,237,524 - MMJJ_RS06705 0.47 +0.2 1,237,524 - MMJJ_RS06705 0.47 -1.6 1,237,524 - MMJJ_RS06705 0.47 -1.3 1,237,526 - MMJJ_RS06705 0.47 +0.2 1,237,638 + MMJJ_RS06705 0.53 -0.8 1,237,658 - MMJJ_RS06705 0.54 -0.8 1,237,664 + MMJJ_RS06705 0.55 +0.2 1,237,841 - MMJJ_RS06705 0.65 -2.6 1,237,842 + MMJJ_RS06705 0.65 +0.2 1,238,035 + MMJJ_RS06705 0.77 -1.8 1,238,076 - -1.7 1,238,094 + MMJJ_RS06705 0.80 -2.1 1,238,149 - MMJJ_RS06705 0.83 +0.3 1,238,296 - -2.5 1,238,296 - -1.3 1,238,377 - -1.7 1,238,420 - -0.4 1,238,421 + +2.9 1,238,426 - -2.4 1,238,426 - -0.3 1,238,426 - -1.6 1,238,431 - +2.5 1,238,431 - -0.4 1,238,492 - MMJJ_RS09325 0.17 +2.1 1,238,499 - MMJJ_RS09325 0.21 -1.3 1,238,500 + MMJJ_RS09325 0.22 +0.1 1,238,510 + MMJJ_RS09325 0.28 -0.0 1,238,517 + MMJJ_RS09325 0.33 +0.3 1,238,522 - MMJJ_RS09325 0.36 -0.2 1,238,531 - MMJJ_RS09325 0.42 +2.3 1,238,531 - MMJJ_RS09325 0.42 +1.3 1,238,535 + MMJJ_RS09325 0.44 +0.6 1,238,550 - MMJJ_RS09325 0.53 -0.0 1,238,550 - MMJJ_RS09325 0.53 +1.2 1,238,551 + MMJJ_RS09325 0.54 -0.0 1,238,561 - MMJJ_RS09325 0.60 +1.0 1,238,561 - MMJJ_RS09325 0.60 +0.3 1,238,562 + MMJJ_RS09325 0.61 -0.7 1,238,562 + MMJJ_RS09325 0.61 +0.3 1,238,566 - MMJJ_RS09325 0.64 -0.8 1,238,567 + MMJJ_RS09325 0.64 +2.1 1,238,598 + MMJJ_RS09325 0.84 -0.4 1,238,638 + +0.7 1,238,660 + +0.9 1,238,742 + +0.4 1,238,848 - MMJJ_RS06710 0.15 +0.3 1,238,906 - MMJJ_RS06710 0.20 +0.3 1,238,907 + MMJJ_RS06710 0.20 -1.1 1,238,908 - MMJJ_RS06710 0.20 +0.4 1,238,921 + MMJJ_RS06710 0.21 +0.1 1,238,927 - MMJJ_RS06710 0.21 +2.2 1,238,959 - MMJJ_RS06710 0.23 +0.5 1,238,959 - MMJJ_RS06710 0.23 +0.8 1,238,964 - MMJJ_RS06710 0.24 +1.1 1,238,964 - MMJJ_RS06710 0.24 +1.0 1,238,964 - MMJJ_RS06710 0.24 -0.0 1,238,964 - MMJJ_RS06710 0.24 +0.1 1,238,964 - MMJJ_RS06710 0.24 -0.5 1,238,964 - MMJJ_RS06710 0.24 +0.2 1,238,964 - MMJJ_RS06710 0.24 +2.9 1,238,964 - MMJJ_RS06710 0.24 -0.6 1,238,965 + MMJJ_RS06710 0.24 -0.3 1,238,965 + MMJJ_RS06710 0.24 +0.4 1,238,965 + MMJJ_RS06710 0.24 -0.2 1,238,965 + MMJJ_RS06710 0.24 -0.2 1,238,965 + MMJJ_RS06710 0.24 +0.7 1,238,965 + MMJJ_RS06710 0.24 +0.3 1,238,965 + MMJJ_RS06710 0.24 +0.4 1,238,968 - MMJJ_RS06710 0.24 +2.1 1,238,968 - MMJJ_RS06710 0.24 +1.5 1,238,968 - MMJJ_RS06710 0.24 +0.6 1,238,968 - MMJJ_RS06710 0.24 +0.0 1,238,969 + MMJJ_RS06710 0.24 -1.6 1,238,969 + MMJJ_RS06710 0.24 +1.1 1,238,969 + MMJJ_RS06710 0.24 -0.7 1,238,969 + MMJJ_RS06710 0.24 -0.2 1,238,971 - MMJJ_RS06710 0.24 +0.5 1,238,972 + MMJJ_RS06710 0.24 +0.7 1,238,992 - MMJJ_RS06710 0.26 +0.2 1,238,992 - MMJJ_RS06710 0.26 +1.1 1,238,997 + MMJJ_RS06710 0.26 -2.3 1,239,067 + MMJJ_RS06710 0.31 +0.4 1,239,067 + MMJJ_RS06710 0.31 +1.6 1,239,074 - MMJJ_RS06710 0.32 +2.6 1,239,112 - MMJJ_RS06710 0.35 -0.1 1,239,136 + MMJJ_RS06710 0.36 +0.4 1,239,173 - MMJJ_RS06710 0.39 +0.5 1,239,180 + MMJJ_RS06710 0.40 +0.2 1,239,285 + MMJJ_RS06710 0.47 +0.3 1,239,406 - MMJJ_RS06710 0.56 -0.9
Or see this region's nucleotide sequence