Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00030 and MMJJ_RS00035 are separated by 159 nucleotides MMJJ_RS00035 and MMJJ_RS00040 are separated by 9 nucleotides MMJJ_RS00040 and MMJJ_RS00045 are separated by 11 nucleotides
MMJJ_RS00030: MMJJ_RS00030 - thymidylate synthase, at 5,374 to 6,249
_RS00030
MMJJ_RS00035: MMJJ_RS00035 - CO dehydrogenase/acetyl-CoA synthase complex subunit alpha, at 6,409 to 8,751
_RS00035
MMJJ_RS00040: MMJJ_RS00040 - CO dehydrogenase/acetyl-CoA synthase complex subunit epsilon, at 8,761 to 9,279
_RS00040
MMJJ_RS00045: MMJJ_RS00045 - CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta, at 9,291 to 10,664
_RS00045
Position (kb) 6
7
8
9 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 5.418 kb on + strand at 5.433 kb on + strand at 5.491 kb on - strand, within MMJJ_RS00030 at 5.513 kb on + strand, within MMJJ_RS00030 at 5.572 kb on + strand, within MMJJ_RS00030 at 5.574 kb on - strand, within MMJJ_RS00030 at 5.582 kb on - strand, within MMJJ_RS00030 at 5.582 kb on - strand, within MMJJ_RS00030 at 5.583 kb on + strand, within MMJJ_RS00030 at 5.583 kb on + strand, within MMJJ_RS00030 at 5.585 kb on - strand, within MMJJ_RS00030 at 5.585 kb on - strand, within MMJJ_RS00030 at 5.585 kb on - strand, within MMJJ_RS00030 at 5.586 kb on + strand, within MMJJ_RS00030 at 5.586 kb on + strand, within MMJJ_RS00030 at 5.593 kb on + strand, within MMJJ_RS00030 at 5.625 kb on - strand, within MMJJ_RS00030 at 5.652 kb on - strand, within MMJJ_RS00030 at 5.697 kb on + strand, within MMJJ_RS00030 at 5.745 kb on - strand, within MMJJ_RS00030 at 5.745 kb on - strand, within MMJJ_RS00030 at 5.749 kb on - strand, within MMJJ_RS00030 at 5.749 kb on - strand, within MMJJ_RS00030 at 5.749 kb on - strand, within MMJJ_RS00030 at 5.750 kb on + strand, within MMJJ_RS00030 at 5.750 kb on + strand, within MMJJ_RS00030 at 5.796 kb on - strand, within MMJJ_RS00030 at 5.808 kb on - strand, within MMJJ_RS00030 at 5.809 kb on + strand, within MMJJ_RS00030 at 5.809 kb on + strand, within MMJJ_RS00030 at 5.814 kb on - strand, within MMJJ_RS00030 at 5.828 kb on - strand, within MMJJ_RS00030 at 5.828 kb on - strand, within MMJJ_RS00030 at 5.829 kb on + strand, within MMJJ_RS00030 at 5.860 kb on - strand, within MMJJ_RS00030 at 5.860 kb on - strand, within MMJJ_RS00030 at 5.860 kb on - strand, within MMJJ_RS00030 at 6.005 kb on - strand, within MMJJ_RS00030 at 6.057 kb on + strand, within MMJJ_RS00030 at 6.061 kb on + strand, within MMJJ_RS00030 at 6.105 kb on + strand, within MMJJ_RS00030 at 6.117 kb on - strand, within MMJJ_RS00030 at 6.158 kb on - strand, within MMJJ_RS00030 at 6.158 kb on - strand, within MMJJ_RS00030 at 6.159 kb on + strand, within MMJJ_RS00030 at 6.165 kb on + strand at 6.205 kb on + strand at 6.205 kb on + strand at 6.205 kb on + strand at 6.206 kb on - strand at 6.206 kb on - strand at 6.206 kb on - strand at 6.206 kb on - strand at 6.207 kb on + strand at 6.207 kb on + strand at 6.246 kb on + strand at 6.259 kb on + strand at 6.265 kb on + strand at 6.268 kb on - strand at 6.325 kb on + strand at 6.335 kb on + strand at 6.365 kb on - strand at 6.365 kb on - strand at 6.365 kb on - strand at 6.365 kb on - strand at 6.366 kb on + strand at 6.366 kb on + strand at 6.366 kb on + strand at 6.366 kb on + strand at 6.366 kb on + strand at 6.367 kb on - strand at 6.368 kb on + strand at 6.369 kb on - strand at 6.370 kb on + strand at 6.371 kb on - strand at 6.371 kb on - strand at 6.372 kb on + strand at 6.372 kb on + strand at 6.372 kb on + strand at 6.372 kb on + strand at 6.423 kb on - strand at 6.466 kb on + strand at 6.591 kb on + strand at 6.627 kb on - strand at 6.627 kb on - strand at 6.639 kb on + strand at 6.642 kb on - strand at 6.642 kb on - strand at 6.643 kb on + strand at 6.643 kb on + strand at 6.643 kb on + strand at 6.662 kb on - strand, within MMJJ_RS00035 at 6.785 kb on - strand, within MMJJ_RS00035 at 6.786 kb on + strand, within MMJJ_RS00035 at 6.815 kb on - strand, within MMJJ_RS00035 at 6.815 kb on - strand, within MMJJ_RS00035 at 6.815 kb on - strand, within MMJJ_RS00035 at 6.815 kb on - strand, within MMJJ_RS00035 at 6.816 kb on + strand, within MMJJ_RS00035 at 6.819 kb on - strand at 6.819 kb on - strand, within MMJJ_RS00035 at 6.839 kb on - strand, within MMJJ_RS00035 at 6.839 kb on - strand, within MMJJ_RS00035 at 6.839 kb on - strand, within MMJJ_RS00035 at 6.882 kb on + strand, within MMJJ_RS00035 at 6.991 kb on + strand, within MMJJ_RS00035 at 7.034 kb on - strand, within MMJJ_RS00035 at 7.035 kb on + strand, within MMJJ_RS00035 at 7.044 kb on - strand, within MMJJ_RS00035 at 7.060 kb on - strand, within MMJJ_RS00035 at 7.097 kb on - strand, within MMJJ_RS00035 at 7.118 kb on - strand, within MMJJ_RS00035 at 7.118 kb on - strand, within MMJJ_RS00035 at 7.122 kb on - strand, within MMJJ_RS00035 at 7.146 kb on - strand, within MMJJ_RS00035 at 7.160 kb on - strand, within MMJJ_RS00035 at 7.240 kb on + strand at 7.248 kb on + strand, within MMJJ_RS00035 at 7.251 kb on - strand, within MMJJ_RS00035 at 7.251 kb on - strand, within MMJJ_RS00035 at 7.251 kb on - strand, within MMJJ_RS00035 at 7.252 kb on + strand, within MMJJ_RS00035 at 7.252 kb on + strand, within MMJJ_RS00035 at 7.252 kb on + strand, within MMJJ_RS00035 at 7.252 kb on + strand, within MMJJ_RS00035 at 7.253 kb on - strand, within MMJJ_RS00035 at 7.253 kb on - strand, within MMJJ_RS00035 at 7.254 kb on + strand, within MMJJ_RS00035 at 7.360 kb on + strand, within MMJJ_RS00035 at 7.384 kb on + strand, within MMJJ_RS00035 at 7.509 kb on - strand, within MMJJ_RS00035 at 7.599 kb on - strand, within MMJJ_RS00035 at 7.600 kb on + strand, within MMJJ_RS00035 at 7.632 kb on - strand, within MMJJ_RS00035 at 7.677 kb on - strand, within MMJJ_RS00035 at 7.677 kb on - strand, within MMJJ_RS00035 at 7.677 kb on - strand, within MMJJ_RS00035 at 7.678 kb on + strand, within MMJJ_RS00035 at 7.735 kb on - strand, within MMJJ_RS00035 at 7.746 kb on - strand, within MMJJ_RS00035 at 7.747 kb on + strand, within MMJJ_RS00035 at 7.753 kb on - strand, within MMJJ_RS00035 at 7.800 kb on + strand, within MMJJ_RS00035 at 7.937 kb on - strand, within MMJJ_RS00035 at 8.066 kb on + strand, within MMJJ_RS00035 at 8.066 kb on + strand, within MMJJ_RS00035 at 8.066 kb on + strand, within MMJJ_RS00035 at 8.066 kb on + strand, within MMJJ_RS00035 at 8.093 kb on + strand, within MMJJ_RS00035 at 8.101 kb on - strand, within MMJJ_RS00035 at 8.136 kb on - strand, within MMJJ_RS00035 at 8.137 kb on + strand, within MMJJ_RS00035 at 8.164 kb on + strand, within MMJJ_RS00035 at 8.164 kb on + strand, within MMJJ_RS00035 at 8.164 kb on + strand, within MMJJ_RS00035 at 8.179 kb on + strand, within MMJJ_RS00035 at 8.242 kb on - strand, within MMJJ_RS00035 at 8.242 kb on - strand, within MMJJ_RS00035 at 8.242 kb on - strand, within MMJJ_RS00035 at 8.243 kb on + strand, within MMJJ_RS00035 at 8.243 kb on + strand, within MMJJ_RS00035 at 8.244 kb on - strand, within MMJJ_RS00035 at 8.245 kb on + strand, within MMJJ_RS00035 at 8.245 kb on + strand, within MMJJ_RS00035 at 8.249 kb on - strand, within MMJJ_RS00035 at 8.250 kb on + strand, within MMJJ_RS00035 at 8.335 kb on + strand, within MMJJ_RS00035 at 8.380 kb on - strand, within MMJJ_RS00035 at 8.380 kb on - strand, within MMJJ_RS00035 at 8.426 kb on - strand, within MMJJ_RS00035 at 8.507 kb on - strand, within MMJJ_RS00035 at 8.507 kb on - strand, within MMJJ_RS00035 at 8.508 kb on + strand, within MMJJ_RS00035 at 8.528 kb on - strand at 8.547 kb on - strand at 8.562 kb on + strand at 8.579 kb on - strand at 8.579 kb on - strand at 8.579 kb on - strand at 8.580 kb on + strand at 8.585 kb on - strand at 8.596 kb on - strand at 8.622 kb on + strand at 8.627 kb on - strand at 8.628 kb on + strand at 8.749 kb on - strand at 8.772 kb on - strand at 8.773 kb on + strand at 8.799 kb on + strand at 8.799 kb on + strand at 8.829 kb on + strand, within MMJJ_RS00040 at 8.897 kb on - strand, within MMJJ_RS00040 at 8.954 kb on - strand, within MMJJ_RS00040 at 8.955 kb on + strand, within MMJJ_RS00040 at 9.038 kb on - strand, within MMJJ_RS00040 at 9.039 kb on + strand, within MMJJ_RS00040 at 9.118 kb on - strand, within MMJJ_RS00040 at 9.118 kb on - strand, within MMJJ_RS00040 at 9.118 kb on - strand, within MMJJ_RS00040 at 9.119 kb on + strand, within MMJJ_RS00040 at 9.119 kb on + strand, within MMJJ_RS00040 at 9.121 kb on - strand, within MMJJ_RS00040 at 9.122 kb on + strand, within MMJJ_RS00040 at 9.122 kb on + strand, within MMJJ_RS00040 at 9.122 kb on + strand, within MMJJ_RS00040 at 9.194 kb on + strand, within MMJJ_RS00040 at 9.194 kb on + strand, within MMJJ_RS00040 at 9.228 kb on - strand at 9.228 kb on - strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.229 kb on + strand at 9.253 kb on - strand at 9.304 kb on - strand at 9.305 kb on + strand at 9.316 kb on - strand at 9.317 kb on + strand at 9.327 kb on - strand at 9.327 kb on - strand at 9.328 kb on + strand at 9.407 kb on + strand at 9.422 kb on + strand at 9.545 kb on + strand, within MMJJ_RS00045 at 9.576 kb on - strand, within MMJJ_RS00045 at 9.576 kb on - strand, within MMJJ_RS00045 at 9.576 kb on - strand, within MMJJ_RS00045 at 9.591 kb on + strand, within MMJJ_RS00045 at 9.598 kb on - strand, within MMJJ_RS00045 at 9.599 kb on + strand, within MMJJ_RS00045 at 9.599 kb on + strand, within MMJJ_RS00045 at 9.661 kb on - strand, within MMJJ_RS00045 at 9.736 kb on - strand, within MMJJ_RS00045 at 9.736 kb on - strand, within MMJJ_RS00045
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 5,418 + +1.5 5,433 + -0.8 5,491 - MMJJ_RS00030 0.13 +0.5 5,513 + MMJJ_RS00030 0.16 -1.7 5,572 + MMJJ_RS00030 0.23 +0.1 5,574 - MMJJ_RS00030 0.23 +0.2 5,582 - MMJJ_RS00030 0.24 +2.0 5,582 - MMJJ_RS00030 0.24 +2.3 5,583 + MMJJ_RS00030 0.24 -1.6 5,583 + MMJJ_RS00030 0.24 -0.8 5,585 - MMJJ_RS00030 0.24 -0.4 5,585 - MMJJ_RS00030 0.24 +0.0 5,585 - MMJJ_RS00030 0.24 +1.2 5,586 + MMJJ_RS00030 0.24 +0.2 5,586 + MMJJ_RS00030 0.24 -0.3 5,593 + MMJJ_RS00030 0.25 +0.3 5,625 - MMJJ_RS00030 0.29 -1.5 5,652 - MMJJ_RS00030 0.32 -1.0 5,697 + MMJJ_RS00030 0.37 +0.1 5,745 - MMJJ_RS00030 0.42 -0.7 5,745 - MMJJ_RS00030 0.42 +1.2 5,749 - MMJJ_RS00030 0.43 +0.7 5,749 - MMJJ_RS00030 0.43 +0.3 5,749 - MMJJ_RS00030 0.43 +0.0 5,750 + MMJJ_RS00030 0.43 +0.9 5,750 + MMJJ_RS00030 0.43 +0.5 5,796 - MMJJ_RS00030 0.48 +0.1 5,808 - MMJJ_RS00030 0.50 -0.5 5,809 + MMJJ_RS00030 0.50 -0.1 5,809 + MMJJ_RS00030 0.50 -0.4 5,814 - MMJJ_RS00030 0.50 -0.4 5,828 - MMJJ_RS00030 0.52 +2.4 5,828 - MMJJ_RS00030 0.52 +0.6 5,829 + MMJJ_RS00030 0.52 -0.1 5,860 - MMJJ_RS00030 0.55 -0.3 5,860 - MMJJ_RS00030 0.55 +0.4 5,860 - MMJJ_RS00030 0.55 +1.1 6,005 - MMJJ_RS00030 0.72 -0.0 6,057 + MMJJ_RS00030 0.78 +0.5 6,061 + MMJJ_RS00030 0.78 +0.6 6,105 + MMJJ_RS00030 0.83 +1.2 6,117 - MMJJ_RS00030 0.85 +2.1 6,158 - MMJJ_RS00030 0.89 +0.1 6,158 - MMJJ_RS00030 0.89 -0.4 6,159 + MMJJ_RS00030 0.90 +0.3 6,165 + -0.7 6,205 + -0.7 6,205 + +1.0 6,205 + +0.8 6,206 - -0.2 6,206 - +0.0 6,206 - -0.5 6,206 - -1.1 6,207 + -1.0 6,207 + -0.5 6,246 + +0.8 6,259 + +1.0 6,265 + +0.9 6,268 - -2.4 6,325 + +2.0 6,335 + -0.5 6,365 - -2.3 6,365 - -3.3 6,365 - -4.6 6,365 - -3.9 6,366 + -3.4 6,366 + -5.0 6,366 + -3.4 6,366 + -2.6 6,366 + -4.1 6,367 - -5.2 6,368 + +0.7 6,369 - -4.0 6,370 + -4.2 6,371 - -4.2 6,371 - -4.1 6,372 + -4.4 6,372 + -3.3 6,372 + -2.7 6,372 + -4.2 6,423 - -5.3 6,466 + -4.4 6,591 + -3.9 6,627 - -4.0 6,627 - -4.8 6,639 + -4.7 6,642 - -3.3 6,642 - -5.6 6,643 + -4.2 6,643 + -1.1 6,643 + -4.9 6,662 - MMJJ_RS00035 0.11 -3.1 6,785 - MMJJ_RS00035 0.16 -3.3 6,786 + MMJJ_RS00035 0.16 -3.3 6,815 - MMJJ_RS00035 0.17 -4.0 6,815 - MMJJ_RS00035 0.17 -4.9 6,815 - MMJJ_RS00035 0.17 -4.0 6,815 - MMJJ_RS00035 0.17 -4.6 6,816 + MMJJ_RS00035 0.17 -3.8 6,819 - -0.1 6,819 - MMJJ_RS00035 0.17 -4.0 6,839 - MMJJ_RS00035 0.18 -4.9 6,839 - MMJJ_RS00035 0.18 -5.0 6,839 - MMJJ_RS00035 0.18 -4.3 6,882 + MMJJ_RS00035 0.20 -3.8 6,991 + MMJJ_RS00035 0.25 -4.2 7,034 - MMJJ_RS00035 0.27 -3.6 7,035 + MMJJ_RS00035 0.27 -3.1 7,044 - MMJJ_RS00035 0.27 -5.0 7,060 - MMJJ_RS00035 0.28 -5.1 7,097 - MMJJ_RS00035 0.29 -3.1 7,118 - MMJJ_RS00035 0.30 -3.7 7,118 - MMJJ_RS00035 0.30 -5.2 7,122 - MMJJ_RS00035 0.30 -2.9 7,146 - MMJJ_RS00035 0.31 -5.3 7,160 - MMJJ_RS00035 0.32 -2.8 7,240 + -1.7 7,248 + MMJJ_RS00035 0.36 -5.0 7,251 - MMJJ_RS00035 0.36 -3.9 7,251 - MMJJ_RS00035 0.36 -4.2 7,251 - MMJJ_RS00035 0.36 -4.8 7,252 + MMJJ_RS00035 0.36 -4.4 7,252 + MMJJ_RS00035 0.36 -0.0 7,252 + MMJJ_RS00035 0.36 -3.9 7,252 + MMJJ_RS00035 0.36 -3.3 7,253 - MMJJ_RS00035 0.36 -4.6 7,253 - MMJJ_RS00035 0.36 -4.5 7,254 + MMJJ_RS00035 0.36 -1.1 7,360 + MMJJ_RS00035 0.41 -3.8 7,384 + MMJJ_RS00035 0.42 -4.1 7,509 - MMJJ_RS00035 0.47 -6.1 7,599 - MMJJ_RS00035 0.51 -0.1 7,600 + MMJJ_RS00035 0.51 -3.1 7,632 - MMJJ_RS00035 0.52 -5.2 7,677 - MMJJ_RS00035 0.54 -4.2 7,677 - MMJJ_RS00035 0.54 -0.7 7,677 - MMJJ_RS00035 0.54 -2.7 7,678 + MMJJ_RS00035 0.54 -5.1 7,735 - MMJJ_RS00035 0.57 -3.3 7,746 - MMJJ_RS00035 0.57 -5.8 7,747 + MMJJ_RS00035 0.57 -4.4 7,753 - MMJJ_RS00035 0.57 -4.1 7,800 + MMJJ_RS00035 0.59 -4.3 7,937 - MMJJ_RS00035 0.65 -0.1 8,066 + MMJJ_RS00035 0.71 -3.3 8,066 + MMJJ_RS00035 0.71 -4.4 8,066 + MMJJ_RS00035 0.71 -4.9 8,066 + MMJJ_RS00035 0.71 -4.8 8,093 + MMJJ_RS00035 0.72 -3.9 8,101 - MMJJ_RS00035 0.72 -4.3 8,136 - MMJJ_RS00035 0.74 -3.6 8,137 + MMJJ_RS00035 0.74 -3.6 8,164 + MMJJ_RS00035 0.75 -4.4 8,164 + MMJJ_RS00035 0.75 -4.8 8,164 + MMJJ_RS00035 0.75 -3.3 8,179 + MMJJ_RS00035 0.76 -5.0 8,242 - MMJJ_RS00035 0.78 -5.6 8,242 - MMJJ_RS00035 0.78 -4.1 8,242 - MMJJ_RS00035 0.78 -6.3 8,243 + MMJJ_RS00035 0.78 -4.7 8,243 + MMJJ_RS00035 0.78 -5.0 8,244 - MMJJ_RS00035 0.78 -4.6 8,245 + MMJJ_RS00035 0.78 -3.9 8,245 + MMJJ_RS00035 0.78 -2.7 8,249 - MMJJ_RS00035 0.79 -3.6 8,250 + MMJJ_RS00035 0.79 -3.1 8,335 + MMJJ_RS00035 0.82 -3.9 8,380 - MMJJ_RS00035 0.84 -4.6 8,380 - MMJJ_RS00035 0.84 -2.9 8,426 - MMJJ_RS00035 0.86 -4.9 8,507 - MMJJ_RS00035 0.90 -6.3 8,507 - MMJJ_RS00035 0.90 -5.2 8,508 + MMJJ_RS00035 0.90 -4.4 8,528 - -4.1 8,547 - -2.1 8,562 + -4.0 8,579 - -4.5 8,579 - -5.6 8,579 - -3.0 8,580 + -4.0 8,585 - -3.6 8,596 - -2.7 8,622 + -5.2 8,627 - -5.0 8,628 + -5.1 8,749 - -3.3 8,772 - -3.3 8,773 + -2.4 8,799 + -4.7 8,799 + -1.1 8,829 + MMJJ_RS00040 0.13 -5.6 8,897 - MMJJ_RS00040 0.26 -5.9 8,954 - MMJJ_RS00040 0.37 -3.3 8,955 + MMJJ_RS00040 0.37 -4.5 9,038 - MMJJ_RS00040 0.53 -5.2 9,039 + MMJJ_RS00040 0.54 -5.2 9,118 - MMJJ_RS00040 0.69 -4.6 9,118 - MMJJ_RS00040 0.69 -4.4 9,118 - MMJJ_RS00040 0.69 -4.0 9,119 + MMJJ_RS00040 0.69 -5.2 9,119 + MMJJ_RS00040 0.69 -2.7 9,121 - MMJJ_RS00040 0.69 -2.9 9,122 + MMJJ_RS00040 0.70 -3.1 9,122 + MMJJ_RS00040 0.70 -4.2 9,122 + MMJJ_RS00040 0.70 -4.6 9,194 + MMJJ_RS00040 0.83 -4.8 9,194 + MMJJ_RS00040 0.83 -2.1 9,228 - -5.6 9,228 - -4.0 9,229 + -2.4 9,229 + -4.5 9,229 + -4.8 9,229 + -3.9 9,229 + -3.4 9,253 - -5.8 9,304 - -2.9 9,305 + -2.4 9,316 - -4.4 9,317 + -4.9 9,327 - -5.1 9,327 - -1.8 9,328 + -5.4 9,407 + -5.0 9,422 + -2.9 9,545 + MMJJ_RS00045 0.18 -5.5 9,576 - MMJJ_RS00045 0.21 -2.1 9,576 - MMJJ_RS00045 0.21 -5.1 9,576 - MMJJ_RS00045 0.21 -3.4 9,591 + MMJJ_RS00045 0.22 -2.9 9,598 - MMJJ_RS00045 0.22 -1.1 9,599 + MMJJ_RS00045 0.22 -4.2 9,599 + MMJJ_RS00045 0.22 -3.1 9,661 - MMJJ_RS00045 0.27 -4.3 9,736 - MMJJ_RS00045 0.32 -2.1 9,736 - MMJJ_RS00045 0.32 -5.2
Or see this region's nucleotide sequence