Experiment: R2A with Selective_ion_mix_1 1 X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS10355 and LRK54_RS10360 are separated by 120 nucleotides LRK54_RS10360 and LRK54_RS10365 overlap by 4 nucleotides
LRK54_RS10355: LRK54_RS10355 - threonine synthase, at 2,281,642 to 2,282,952
_RS10355
LRK54_RS10360: LRK54_RS10360 - homoserine kinase, at 2,283,073 to 2,284,068
_RS10360
LRK54_RS10365: LRK54_RS10365 - homoserine dehydrogenase, at 2,284,065 to 2,285,210
_RS10365
Position (kb)
2283
2284
2285 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2282.258 kb on + strand, within LRK54_RS10355 at 2282.259 kb on - strand, within LRK54_RS10355 at 2282.259 kb on - strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.261 kb on + strand, within LRK54_RS10355 at 2282.262 kb on - strand, within LRK54_RS10355 at 2282.262 kb on - strand, within LRK54_RS10355 at 2282.262 kb on - strand, within LRK54_RS10355 at 2282.262 kb on - strand, within LRK54_RS10355 at 2282.852 kb on + strand at 2282.852 kb on + strand at 2282.852 kb on + strand at 2282.852 kb on + strand at 2282.852 kb on + strand at 2282.852 kb on + strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.853 kb on - strand at 2282.933 kb on + strand at 2283.218 kb on + strand, within LRK54_RS10360 at 2283.218 kb on + strand, within LRK54_RS10360 at 2283.218 kb on + strand, within LRK54_RS10360 at 2283.218 kb on + strand, within LRK54_RS10360 at 2283.219 kb on - strand, within LRK54_RS10360 at 2283.219 kb on - strand, within LRK54_RS10360 at 2283.219 kb on - strand, within LRK54_RS10360 at 2283.219 kb on - strand, within LRK54_RS10360 at 2283.444 kb on - strand, within LRK54_RS10360 at 2283.444 kb on - strand, within LRK54_RS10360 at 2283.647 kb on + strand, within LRK54_RS10360 at 2283.647 kb on + strand, within LRK54_RS10360 at 2283.648 kb on - strand, within LRK54_RS10360 at 2283.648 kb on - strand, within LRK54_RS10360 at 2284.177 kb on + strand at 2284.177 kb on + strand at 2284.178 kb on - strand at 2284.260 kb on + strand, within LRK54_RS10365 at 2284.261 kb on - strand, within LRK54_RS10365 at 2284.261 kb on - strand, within LRK54_RS10365 at 2284.261 kb on - strand, within LRK54_RS10365 at 2284.261 kb on - strand, within LRK54_RS10365 at 2284.261 kb on - strand, within LRK54_RS10365 at 2284.532 kb on + strand, within LRK54_RS10365 at 2284.532 kb on + strand, within LRK54_RS10365 at 2284.532 kb on + strand, within LRK54_RS10365 at 2284.533 kb on - strand, within LRK54_RS10365 at 2284.533 kb on - strand, within LRK54_RS10365 at 2284.534 kb on + strand, within LRK54_RS10365 at 2284.534 kb on + strand, within LRK54_RS10365 at 2284.534 kb on + strand, within LRK54_RS10365 at 2284.534 kb on + strand, within LRK54_RS10365 at 2284.534 kb on + strand, within LRK54_RS10365 at 2284.534 kb on + strand, within LRK54_RS10365 at 2284.535 kb on - strand, within LRK54_RS10365 at 2284.535 kb on - strand, within LRK54_RS10365 at 2284.715 kb on - strand, within LRK54_RS10365
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Selective_ion_mix_1 1 X remove 2,282,258 + LRK54_RS10355 0.47 +0.3 2,282,259 - LRK54_RS10355 0.47 +0.9 2,282,259 - LRK54_RS10355 0.47 +1.2 2,282,261 + LRK54_RS10355 0.47 +1.3 2,282,261 + LRK54_RS10355 0.47 +0.3 2,282,261 + LRK54_RS10355 0.47 +0.9 2,282,261 + LRK54_RS10355 0.47 +1.2 2,282,261 + LRK54_RS10355 0.47 +1.4 2,282,261 + LRK54_RS10355 0.47 +0.8 2,282,262 - LRK54_RS10355 0.47 +1.0 2,282,262 - LRK54_RS10355 0.47 +0.2 2,282,262 - LRK54_RS10355 0.47 +1.3 2,282,262 - LRK54_RS10355 0.47 +2.0 2,282,852 + -1.7 2,282,852 + +0.2 2,282,852 + +2.0 2,282,852 + -1.5 2,282,852 + +1.1 2,282,852 + -0.4 2,282,853 - +1.6 2,282,853 - -0.3 2,282,853 - +0.4 2,282,853 - +1.6 2,282,853 - +1.4 2,282,853 - -0.2 2,282,853 - +0.8 2,282,853 - +0.5 2,282,933 + +1.5 2,283,218 + LRK54_RS10360 0.15 -1.1 2,283,218 + LRK54_RS10360 0.15 -0.5 2,283,218 + LRK54_RS10360 0.15 +0.4 2,283,218 + LRK54_RS10360 0.15 -0.1 2,283,219 - LRK54_RS10360 0.15 -0.0 2,283,219 - LRK54_RS10360 0.15 +0.1 2,283,219 - LRK54_RS10360 0.15 -0.0 2,283,219 - LRK54_RS10360 0.15 +0.3 2,283,444 - LRK54_RS10360 0.37 -0.6 2,283,444 - LRK54_RS10360 0.37 -0.6 2,283,647 + LRK54_RS10360 0.58 -1.4 2,283,647 + LRK54_RS10360 0.58 +0.9 2,283,648 - LRK54_RS10360 0.58 -0.7 2,283,648 - LRK54_RS10360 0.58 +2.2 2,284,177 + -1.4 2,284,177 + +0.8 2,284,178 - -1.1 2,284,260 + LRK54_RS10365 0.17 -2.7 2,284,261 - LRK54_RS10365 0.17 +0.3 2,284,261 - LRK54_RS10365 0.17 -1.0 2,284,261 - LRK54_RS10365 0.17 -1.4 2,284,261 - LRK54_RS10365 0.17 +0.3 2,284,261 - LRK54_RS10365 0.17 -0.7 2,284,532 + LRK54_RS10365 0.41 -0.9 2,284,532 + LRK54_RS10365 0.41 -3.1 2,284,532 + LRK54_RS10365 0.41 +0.4 2,284,533 - LRK54_RS10365 0.41 -1.7 2,284,533 - LRK54_RS10365 0.41 +0.7 2,284,534 + LRK54_RS10365 0.41 -0.2 2,284,534 + LRK54_RS10365 0.41 -0.7 2,284,534 + LRK54_RS10365 0.41 -2.2 2,284,534 + LRK54_RS10365 0.41 -0.0 2,284,534 + LRK54_RS10365 0.41 +0.6 2,284,534 + LRK54_RS10365 0.41 +0.8 2,284,535 - LRK54_RS10365 0.41 -0.3 2,284,535 - LRK54_RS10365 0.41 +1.3 2,284,715 - LRK54_RS10365 0.57 -1.0
Or see this region's nucleotide sequence