Experiment: Paraquat dichloride 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00495 and SMc00494 are separated by 18 nucleotides SMc00494 and SMc00493 are separated by 81 nucleotides SMc00493 and SMc00492 are separated by 105 nucleotides SMc00492 and SMc00491 are separated by 105 nucleotides SMc00491 and SMc00490 are separated by 172 nucleotides
SMc00495: SMc00495 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 1,915,805 to 1,916,569
SMc00495
SMc00494: SMc00494 - phosphoribosylformylglycinamidine synthase subunit PurS, at 1,916,588 to 1,916,830
SMc00494
SMc00493: SMc00493 - phosphoribosylformylglycinamidine synthase I, at 1,916,912 to 1,917,583
SMc00493
SMc00492: SMc00492 - hypothetical protein, at 1,917,689 to 1,918,096
SMc00492
SMc00491: SMc00491 - hypothetical protein, at 1,918,202 to 1,918,354
SMc00491
SMc00490: SMc00490 - transcriptional regulator, at 1,918,527 to 1,919,945
SMc00490
Position (kb)
1916
1917
1918 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1916.165 kb on + strand, within SMc00495 at 1916.168 kb on + strand, within SMc00495 at 1916.354 kb on + strand, within SMc00495 at 1916.355 kb on - strand, within SMc00495 at 1916.567 kb on + strand at 1916.568 kb on - strand at 1916.605 kb on - strand at 1916.794 kb on + strand, within SMc00494 at 1916.810 kb on + strand at 1916.810 kb on + strand at 1916.811 kb on - strand at 1916.811 kb on - strand at 1916.878 kb on + strand at 1916.879 kb on - strand at 1916.879 kb on - strand at 1916.879 kb on - strand at 1916.880 kb on + strand at 1916.880 kb on + strand at 1916.881 kb on - strand at 1916.881 kb on - strand at 1916.881 kb on - strand at 1916.902 kb on + strand at 1916.902 kb on + strand at 1916.903 kb on - strand at 1916.903 kb on - strand at 1916.903 kb on - strand at 1916.903 kb on - strand at 1916.903 kb on - strand at 1916.903 kb on - strand at 1916.903 kb on - strand at 1916.906 kb on + strand at 1916.907 kb on - strand at 1916.907 kb on - strand at 1916.907 kb on - strand at 1917.069 kb on - strand, within SMc00493 at 1917.078 kb on - strand, within SMc00493 at 1917.078 kb on - strand, within SMc00493 at 1917.299 kb on + strand, within SMc00493 at 1917.350 kb on + strand, within SMc00493 at 1917.350 kb on + strand, within SMc00493 at 1917.350 kb on + strand, within SMc00493 at 1917.350 kb on + strand, within SMc00493 at 1917.350 kb on + strand, within SMc00493 at 1917.411 kb on - strand, within SMc00493 at 1917.570 kb on + strand at 1917.571 kb on - strand at 1917.571 kb on - strand at 1917.571 kb on - strand at 1917.571 kb on - strand at 1917.595 kb on + strand at 1917.595 kb on + strand at 1917.688 kb on + strand at 1917.688 kb on + strand at 1917.688 kb on + strand at 1917.688 kb on + strand at 1917.689 kb on - strand at 1917.689 kb on - strand at 1917.689 kb on - strand at 1917.881 kb on + strand, within SMc00492 at 1918.256 kb on + strand, within SMc00491 at 1918.257 kb on - strand, within SMc00491 at 1918.257 kb on - strand, within SMc00491 at 1918.257 kb on - strand, within SMc00491 at 1918.257 kb on - strand, within SMc00491 at 1918.258 kb on + strand, within SMc00491 at 1918.259 kb on - strand, within SMc00491 at 1918.259 kb on - strand, within SMc00491 at 1918.329 kb on + strand, within SMc00491 at 1918.329 kb on + strand, within SMc00491 at 1918.330 kb on - strand, within SMc00491 at 1918.330 kb on - strand, within SMc00491 at 1918.486 kb on + strand at 1918.487 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.05 mg/ml remove 1,916,165 + SMc00495 0.47 +3.0 1,916,168 + SMc00495 0.47 +3.0 1,916,354 + SMc00495 0.72 +3.2 1,916,355 - SMc00495 0.72 +1.2 1,916,567 + -0.2 1,916,568 - +0.3 1,916,605 - +0.2 1,916,794 + SMc00494 0.85 -0.1 1,916,810 + +0.2 1,916,810 + +0.1 1,916,811 - -0.4 1,916,811 - -1.4 1,916,878 + -0.5 1,916,879 - +0.2 1,916,879 - +0.4 1,916,879 - -0.1 1,916,880 + +0.9 1,916,880 + -0.7 1,916,881 - -0.1 1,916,881 - +0.1 1,916,881 - -0.1 1,916,902 + +0.4 1,916,902 + -2.7 1,916,903 - -0.5 1,916,903 - +1.0 1,916,903 - +0.4 1,916,903 - +0.4 1,916,903 - -0.1 1,916,903 - +0.7 1,916,903 - +0.9 1,916,906 + -1.1 1,916,907 - +0.8 1,916,907 - -0.6 1,916,907 - +0.7 1,917,069 - SMc00493 0.23 -1.0 1,917,078 - SMc00493 0.25 +0.3 1,917,078 - SMc00493 0.25 +0.7 1,917,299 + SMc00493 0.58 +0.6 1,917,350 + SMc00493 0.65 +0.1 1,917,350 + SMc00493 0.65 -0.1 1,917,350 + SMc00493 0.65 -1.1 1,917,350 + SMc00493 0.65 -0.2 1,917,350 + SMc00493 0.65 +0.6 1,917,411 - SMc00493 0.74 -0.8 1,917,570 + +0.8 1,917,571 - +0.2 1,917,571 - -0.2 1,917,571 - -0.6 1,917,571 - -0.0 1,917,595 + +0.5 1,917,595 + +0.4 1,917,688 + -1.2 1,917,688 + -1.1 1,917,688 + -0.5 1,917,688 + -0.3 1,917,689 - -1.4 1,917,689 - -1.4 1,917,689 - -1.0 1,917,881 + SMc00492 0.47 -2.7 1,918,256 + SMc00491 0.35 +0.4 1,918,257 - SMc00491 0.36 -0.3 1,918,257 - SMc00491 0.36 +0.7 1,918,257 - SMc00491 0.36 -1.1 1,918,257 - SMc00491 0.36 -0.2 1,918,258 + SMc00491 0.37 -0.7 1,918,259 - SMc00491 0.37 +0.8 1,918,259 - SMc00491 0.37 +0.2 1,918,329 + SMc00491 0.83 -0.3 1,918,329 + SMc00491 0.83 -0.5 1,918,330 - SMc00491 0.84 -0.6 1,918,330 - SMc00491 0.84 +0.5 1,918,486 + +1.0 1,918,487 - -0.3
Or see this region's nucleotide sequence