Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1001 and Pf6N2E2_1002 are separated by 92 nucleotides Pf6N2E2_1002 and Pf6N2E2_1003 are separated by 68 nucleotides
Pf6N2E2_1001 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 1,170,296 to 1,171,150
_1001
Pf6N2E2_1002 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 1,171,243 to 1,172,244
_1002
Pf6N2E2_1003 - Fucose permease, at 1,172,313 to 1,173,695
_1003
Position (kb)
1171
1172
1173 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1170.245 kb on + strand at 1170.245 kb on + strand at 1170.246 kb on - strand at 1170.291 kb on + strand at 1170.292 kb on - strand at 1170.330 kb on - strand at 1170.330 kb on - strand at 1170.353 kb on + strand at 1170.402 kb on + strand, within Pf6N2E2_1001 at 1170.403 kb on - strand, within Pf6N2E2_1001 at 1170.425 kb on + strand, within Pf6N2E2_1001 at 1170.587 kb on - strand, within Pf6N2E2_1001 at 1170.587 kb on - strand, within Pf6N2E2_1001 at 1170.657 kb on + strand, within Pf6N2E2_1001 at 1170.658 kb on - strand, within Pf6N2E2_1001 at 1170.676 kb on + strand, within Pf6N2E2_1001 at 1170.728 kb on + strand, within Pf6N2E2_1001 at 1170.728 kb on + strand, within Pf6N2E2_1001 at 1170.729 kb on - strand, within Pf6N2E2_1001 at 1170.763 kb on + strand, within Pf6N2E2_1001 at 1170.776 kb on - strand, within Pf6N2E2_1001 at 1170.776 kb on - strand, within Pf6N2E2_1001 at 1170.934 kb on + strand, within Pf6N2E2_1001 at 1170.935 kb on - strand, within Pf6N2E2_1001 at 1170.935 kb on - strand, within Pf6N2E2_1001 at 1170.938 kb on + strand at 1171.018 kb on + strand, within Pf6N2E2_1001 at 1171.018 kb on + strand, within Pf6N2E2_1001 at 1171.021 kb on + strand, within Pf6N2E2_1001 at 1171.021 kb on + strand, within Pf6N2E2_1001 at 1171.021 kb on + strand, within Pf6N2E2_1001 at 1171.028 kb on - strand, within Pf6N2E2_1001 at 1171.148 kb on + strand at 1171.148 kb on + strand at 1171.148 kb on + strand at 1171.154 kb on + strand at 1171.154 kb on + strand at 1171.154 kb on + strand at 1171.219 kb on + strand at 1171.239 kb on + strand at 1171.239 kb on + strand at 1171.239 kb on + strand at 1171.239 kb on + strand at 1171.239 kb on + strand at 1171.240 kb on - strand at 1171.240 kb on - strand at 1171.334 kb on + strand at 1171.334 kb on + strand at 1171.335 kb on - strand at 1171.396 kb on + strand, within Pf6N2E2_1002 at 1171.396 kb on + strand, within Pf6N2E2_1002 at 1171.402 kb on + strand, within Pf6N2E2_1002 at 1171.402 kb on + strand, within Pf6N2E2_1002 at 1171.403 kb on - strand, within Pf6N2E2_1002 at 1171.422 kb on + strand, within Pf6N2E2_1002 at 1171.481 kb on + strand, within Pf6N2E2_1002 at 1171.509 kb on + strand, within Pf6N2E2_1002 at 1171.537 kb on + strand, within Pf6N2E2_1002 at 1171.537 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.758 kb on + strand, within Pf6N2E2_1002 at 1171.890 kb on + strand, within Pf6N2E2_1002 at 1171.890 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.937 kb on + strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.938 kb on - strand, within Pf6N2E2_1002 at 1171.939 kb on + strand, within Pf6N2E2_1002 at 1171.939 kb on + strand, within Pf6N2E2_1002 at 1171.940 kb on - strand, within Pf6N2E2_1002 at 1172.004 kb on + strand, within Pf6N2E2_1002 at 1172.005 kb on - strand, within Pf6N2E2_1002 at 1172.005 kb on - strand, within Pf6N2E2_1002 at 1172.092 kb on + strand, within Pf6N2E2_1002 at 1172.092 kb on + strand, within Pf6N2E2_1002 at 1172.093 kb on - strand, within Pf6N2E2_1002 at 1172.093 kb on - strand, within Pf6N2E2_1002 at 1172.133 kb on + strand, within Pf6N2E2_1002 at 1172.134 kb on - strand, within Pf6N2E2_1002 at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.225 kb on + strand at 1172.226 kb on - strand at 1172.243 kb on - strand at 1172.247 kb on + strand at 1172.248 kb on - strand at 1172.276 kb on - strand at 1172.276 kb on - strand at 1172.330 kb on - strand at 1172.339 kb on + strand at 1172.340 kb on - strand at 1172.444 kb on + strand at 1172.444 kb on + strand at 1172.508 kb on - strand, within Pf6N2E2_1003 at 1172.594 kb on + strand, within Pf6N2E2_1003 at 1172.594 kb on + strand, within Pf6N2E2_1003 at 1172.595 kb on - strand, within Pf6N2E2_1003 at 1172.722 kb on + strand, within Pf6N2E2_1003 at 1172.723 kb on - strand, within Pf6N2E2_1003 at 1172.775 kb on - strand, within Pf6N2E2_1003 at 1172.927 kb on - strand, within Pf6N2E2_1003 at 1173.009 kb on + strand, within Pf6N2E2_1003 at 1173.009 kb on + strand, within Pf6N2E2_1003 at 1173.010 kb on - strand, within Pf6N2E2_1003 at 1173.010 kb on - strand, within Pf6N2E2_1003 at 1173.010 kb on - strand, within Pf6N2E2_1003 at 1173.125 kb on - strand, within Pf6N2E2_1003 at 1173.125 kb on - strand, within Pf6N2E2_1003 at 1173.125 kb on - strand, within Pf6N2E2_1003 at 1173.189 kb on + strand, within Pf6N2E2_1003 at 1173.189 kb on + strand, within Pf6N2E2_1003 at 1173.190 kb on - strand, within Pf6N2E2_1003 at 1173.219 kb on + strand, within Pf6N2E2_1003
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 1,170,245 + -0.2 1,170,245 + -0.3 1,170,246 - +1.1 1,170,291 + +0.4 1,170,292 - -1.6 1,170,330 - +0.3 1,170,330 - +0.7 1,170,353 + +0.4 1,170,402 + Pf6N2E2_1001 0.12 +2.1 1,170,403 - Pf6N2E2_1001 0.13 +0.4 1,170,425 + Pf6N2E2_1001 0.15 +0.1 1,170,587 - Pf6N2E2_1001 0.34 +0.3 1,170,587 - Pf6N2E2_1001 0.34 +1.0 1,170,657 + Pf6N2E2_1001 0.42 +0.1 1,170,658 - Pf6N2E2_1001 0.42 +0.5 1,170,676 + Pf6N2E2_1001 0.44 -0.0 1,170,728 + Pf6N2E2_1001 0.51 -0.8 1,170,728 + Pf6N2E2_1001 0.51 +0.5 1,170,729 - Pf6N2E2_1001 0.51 +1.4 1,170,763 + Pf6N2E2_1001 0.55 +0.5 1,170,776 - Pf6N2E2_1001 0.56 +0.0 1,170,776 - Pf6N2E2_1001 0.56 -0.0 1,170,934 + Pf6N2E2_1001 0.75 -0.4 1,170,935 - Pf6N2E2_1001 0.75 +0.3 1,170,935 - Pf6N2E2_1001 0.75 -0.7 1,170,938 + +0.4 1,171,018 + Pf6N2E2_1001 0.84 -0.8 1,171,018 + Pf6N2E2_1001 0.84 -0.3 1,171,021 + Pf6N2E2_1001 0.85 +0.1 1,171,021 + Pf6N2E2_1001 0.85 +0.6 1,171,021 + Pf6N2E2_1001 0.85 -0.5 1,171,028 - Pf6N2E2_1001 0.86 -0.5 1,171,148 + -0.1 1,171,148 + +1.0 1,171,148 + +0.3 1,171,154 + +0.7 1,171,154 + -0.9 1,171,154 + -1.0 1,171,219 + -0.9 1,171,239 + +0.4 1,171,239 + -0.5 1,171,239 + +1.4 1,171,239 + +0.8 1,171,239 + +0.2 1,171,240 - +0.0 1,171,240 - +1.1 1,171,334 + +0.6 1,171,334 + -1.2 1,171,335 - -0.3 1,171,396 + Pf6N2E2_1002 0.15 -0.9 1,171,396 + Pf6N2E2_1002 0.15 +0.1 1,171,402 + Pf6N2E2_1002 0.16 +1.0 1,171,402 + Pf6N2E2_1002 0.16 +1.1 1,171,403 - Pf6N2E2_1002 0.16 +0.8 1,171,422 + Pf6N2E2_1002 0.18 -0.2 1,171,481 + Pf6N2E2_1002 0.24 -0.5 1,171,509 + Pf6N2E2_1002 0.27 +0.9 1,171,537 + Pf6N2E2_1002 0.29 -1.8 1,171,537 + Pf6N2E2_1002 0.29 -0.1 1,171,758 + Pf6N2E2_1002 0.51 -1.0 1,171,758 + Pf6N2E2_1002 0.51 +0.2 1,171,758 + Pf6N2E2_1002 0.51 -0.6 1,171,758 + Pf6N2E2_1002 0.51 -0.7 1,171,890 + Pf6N2E2_1002 0.65 -0.6 1,171,890 + Pf6N2E2_1002 0.65 -0.8 1,171,937 + Pf6N2E2_1002 0.69 -0.2 1,171,937 + Pf6N2E2_1002 0.69 +0.9 1,171,937 + Pf6N2E2_1002 0.69 -0.5 1,171,937 + Pf6N2E2_1002 0.69 +0.2 1,171,937 + Pf6N2E2_1002 0.69 -0.4 1,171,938 - Pf6N2E2_1002 0.69 -0.7 1,171,938 - Pf6N2E2_1002 0.69 +0.2 1,171,938 - +2.1 1,171,938 - Pf6N2E2_1002 0.69 +0.4 1,171,938 - Pf6N2E2_1002 0.69 -0.5 1,171,938 - Pf6N2E2_1002 0.69 +0.0 1,171,938 - Pf6N2E2_1002 0.69 +0.6 1,171,938 - Pf6N2E2_1002 0.69 -0.8 1,171,939 + Pf6N2E2_1002 0.69 -0.6 1,171,939 + Pf6N2E2_1002 0.69 +0.5 1,171,940 - Pf6N2E2_1002 0.70 -0.7 1,172,004 + Pf6N2E2_1002 0.76 -0.5 1,172,005 - Pf6N2E2_1002 0.76 -0.3 1,172,005 - Pf6N2E2_1002 0.76 +0.1 1,172,092 + Pf6N2E2_1002 0.85 -0.2 1,172,092 + Pf6N2E2_1002 0.85 +0.9 1,172,093 - Pf6N2E2_1002 0.85 +0.0 1,172,093 - Pf6N2E2_1002 0.85 -0.5 1,172,133 + Pf6N2E2_1002 0.89 -1.0 1,172,134 - Pf6N2E2_1002 0.89 -0.3 1,172,225 + -0.8 1,172,225 + -1.0 1,172,225 + -0.9 1,172,225 + +0.8 1,172,225 + +2.1 1,172,226 - -0.3 1,172,243 - +0.4 1,172,247 + -0.1 1,172,248 - +1.6 1,172,276 - -1.0 1,172,276 - -0.8 1,172,330 - -1.2 1,172,339 + -0.0 1,172,340 - +0.9 1,172,444 + +0.2 1,172,444 + +0.4 1,172,508 - Pf6N2E2_1003 0.14 -1.3 1,172,594 + Pf6N2E2_1003 0.20 -0.3 1,172,594 + Pf6N2E2_1003 0.20 +0.8 1,172,595 - Pf6N2E2_1003 0.20 +1.7 1,172,722 + Pf6N2E2_1003 0.30 +0.1 1,172,723 - Pf6N2E2_1003 0.30 -0.1 1,172,775 - Pf6N2E2_1003 0.33 -0.8 1,172,927 - Pf6N2E2_1003 0.44 +1.5 1,173,009 + Pf6N2E2_1003 0.50 +1.3 1,173,009 + Pf6N2E2_1003 0.50 -1.9 1,173,010 - Pf6N2E2_1003 0.50 -0.4 1,173,010 - Pf6N2E2_1003 0.50 -0.2 1,173,010 - Pf6N2E2_1003 0.50 -1.1 1,173,125 - Pf6N2E2_1003 0.59 -0.4 1,173,125 - Pf6N2E2_1003 0.59 +0.6 1,173,125 - Pf6N2E2_1003 0.59 +0.7 1,173,189 + Pf6N2E2_1003 0.63 -0.3 1,173,189 + Pf6N2E2_1003 0.63 -0.7 1,173,190 - Pf6N2E2_1003 0.63 +0.5 1,173,219 + Pf6N2E2_1003 0.66 -0.2
Or see this region's nucleotide sequence