Experiment: R2A with sodium fluoride 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22640 and CA265_RS22645 are separated by 146 nucleotides CA265_RS22645 and CA265_RS22650 are separated by 146 nucleotides
CA265_RS22640: CA265_RS22640 - chloride channel protein, at 5,543,772 to 5,545,061
_RS22640
CA265_RS22645: CA265_RS22645 - tryptophan 2,3-dioxygenase, at 5,545,208 to 5,546,173
_RS22645
CA265_RS22650: CA265_RS22650 - branched chain amino acid aminotransferase, at 5,546,320 to 5,547,384
_RS22650
Position (kb) 5545
5546
5547 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 5544.232 kb on - strand, within CA265_RS22640 at 5544.334 kb on - strand, within CA265_RS22640 at 5544.337 kb on - strand, within CA265_RS22640 at 5544.358 kb on - strand, within CA265_RS22640 at 5544.360 kb on + strand, within CA265_RS22640 at 5544.435 kb on - strand, within CA265_RS22640 at 5544.437 kb on - strand at 5544.624 kb on - strand, within CA265_RS22640 at 5544.690 kb on + strand, within CA265_RS22640 at 5544.702 kb on - strand, within CA265_RS22640 at 5544.910 kb on - strand, within CA265_RS22640 at 5544.973 kb on - strand at 5545.010 kb on + strand at 5545.158 kb on - strand at 5545.161 kb on + strand at 5545.162 kb on - strand at 5545.197 kb on - strand at 5545.228 kb on - strand at 5545.228 kb on - strand at 5545.239 kb on + strand at 5545.248 kb on + strand at 5545.248 kb on + strand at 5545.260 kb on - strand at 5545.306 kb on - strand, within CA265_RS22645 at 5545.308 kb on - strand, within CA265_RS22645 at 5545.326 kb on - strand, within CA265_RS22645 at 5545.334 kb on + strand, within CA265_RS22645 at 5545.335 kb on - strand, within CA265_RS22645 at 5545.409 kb on + strand, within CA265_RS22645 at 5545.410 kb on - strand, within CA265_RS22645 at 5545.436 kb on - strand, within CA265_RS22645 at 5545.539 kb on + strand, within CA265_RS22645 at 5545.609 kb on - strand, within CA265_RS22645 at 5545.672 kb on + strand, within CA265_RS22645 at 5545.707 kb on + strand, within CA265_RS22645 at 5545.707 kb on + strand, within CA265_RS22645 at 5545.722 kb on + strand, within CA265_RS22645 at 5545.722 kb on + strand, within CA265_RS22645 at 5545.723 kb on - strand, within CA265_RS22645 at 5545.723 kb on - strand, within CA265_RS22645 at 5545.742 kb on - strand, within CA265_RS22645 at 5545.877 kb on + strand, within CA265_RS22645 at 5545.877 kb on + strand, within CA265_RS22645 at 5545.878 kb on - strand, within CA265_RS22645 at 5545.946 kb on + strand, within CA265_RS22645 at 5545.951 kb on - strand, within CA265_RS22645 at 5545.990 kb on - strand, within CA265_RS22645 at 5546.017 kb on + strand, within CA265_RS22645 at 5546.017 kb on + strand, within CA265_RS22645 at 5546.146 kb on - strand at 5546.150 kb on - strand at 5546.298 kb on - strand at 5546.314 kb on - strand at 5546.339 kb on + strand at 5546.544 kb on - strand, within CA265_RS22650 at 5546.552 kb on - strand, within CA265_RS22650 at 5546.575 kb on - strand, within CA265_RS22650 at 5546.662 kb on - strand, within CA265_RS22650 at 5546.723 kb on - strand, within CA265_RS22650 at 5546.729 kb on + strand, within CA265_RS22650 at 5546.731 kb on + strand, within CA265_RS22650 at 5546.732 kb on - strand, within CA265_RS22650 at 5546.732 kb on - strand, within CA265_RS22650 at 5546.805 kb on - strand, within CA265_RS22650 at 5546.831 kb on - strand, within CA265_RS22650 at 5546.831 kb on - strand, within CA265_RS22650 at 5546.914 kb on - strand, within CA265_RS22650 at 5546.930 kb on + strand, within CA265_RS22650 at 5546.931 kb on - strand, within CA265_RS22650 at 5547.004 kb on - strand, within CA265_RS22650 at 5547.103 kb on - strand, within CA265_RS22650 at 5547.135 kb on - strand, within CA265_RS22650 at 5547.151 kb on - strand, within CA265_RS22650
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 25 mM remove 5,544,232 - CA265_RS22640 0.36 -2.4 5,544,334 - CA265_RS22640 0.44 -2.9 5,544,337 - CA265_RS22640 0.44 -2.7 5,544,358 - CA265_RS22640 0.45 -3.0 5,544,360 + CA265_RS22640 0.46 -3.4 5,544,435 - CA265_RS22640 0.51 -2.0 5,544,437 - -0.1 5,544,624 - CA265_RS22640 0.66 -2.9 5,544,690 + CA265_RS22640 0.71 -1.9 5,544,702 - CA265_RS22640 0.72 -2.8 5,544,910 - CA265_RS22640 0.88 -0.9 5,544,973 - -2.6 5,545,010 + -1.4 5,545,158 - +5.8 5,545,161 + -1.9 5,545,162 - +5.0 5,545,197 - +4.8 5,545,228 - +5.1 5,545,228 - +4.3 5,545,239 + +0.1 5,545,248 + -0.5 5,545,248 + -0.2 5,545,260 - +5.3 5,545,306 - CA265_RS22645 0.10 +4.6 5,545,308 - CA265_RS22645 0.10 +4.8 5,545,326 - CA265_RS22645 0.12 +4.2 5,545,334 + CA265_RS22645 0.13 -2.0 5,545,335 - CA265_RS22645 0.13 +4.4 5,545,409 + CA265_RS22645 0.21 +0.3 5,545,410 - CA265_RS22645 0.21 +5.0 5,545,436 - CA265_RS22645 0.24 +5.0 5,545,539 + CA265_RS22645 0.34 -0.2 5,545,609 - CA265_RS22645 0.42 +4.9 5,545,672 + CA265_RS22645 0.48 -1.5 5,545,707 + CA265_RS22645 0.52 -1.2 5,545,707 + CA265_RS22645 0.52 +0.7 5,545,722 + CA265_RS22645 0.53 -0.5 5,545,722 + CA265_RS22645 0.53 -1.0 5,545,723 - CA265_RS22645 0.53 +3.8 5,545,723 - CA265_RS22645 0.53 +3.9 5,545,742 - CA265_RS22645 0.55 +3.9 5,545,877 + CA265_RS22645 0.69 +2.2 5,545,877 + CA265_RS22645 0.69 -0.4 5,545,878 - CA265_RS22645 0.69 +3.3 5,545,946 + CA265_RS22645 0.76 -0.9 5,545,951 - CA265_RS22645 0.77 +2.5 5,545,990 - CA265_RS22645 0.81 +3.0 5,546,017 + CA265_RS22645 0.84 +0.5 5,546,017 + CA265_RS22645 0.84 +0.1 5,546,146 - +0.7 5,546,150 - +1.5 5,546,298 - -0.3 5,546,314 - -2.4 5,546,339 + -1.4 5,546,544 - CA265_RS22650 0.21 +0.6 5,546,552 - CA265_RS22650 0.22 +0.3 5,546,575 - CA265_RS22650 0.24 -0.8 5,546,662 - CA265_RS22650 0.32 +1.0 5,546,723 - CA265_RS22650 0.38 +0.6 5,546,729 + CA265_RS22650 0.38 -0.7 5,546,731 + CA265_RS22650 0.39 -1.1 5,546,732 - CA265_RS22650 0.39 -1.3 5,546,732 - CA265_RS22650 0.39 -0.4 5,546,805 - CA265_RS22650 0.46 -0.1 5,546,831 - CA265_RS22650 0.48 -0.7 5,546,831 - CA265_RS22650 0.48 -0.7 5,546,914 - CA265_RS22650 0.56 -2.1 5,546,930 + CA265_RS22650 0.57 -1.4 5,546,931 - CA265_RS22650 0.57 -0.9 5,547,004 - CA265_RS22650 0.64 -0.3 5,547,103 - CA265_RS22650 0.74 -1.4 5,547,135 - CA265_RS22650 0.77 +1.3 5,547,151 - CA265_RS22650 0.78 -1.3
Or see this region's nucleotide sequence