Experiment: R2A with sodium fluoride 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14990 and CA265_RS14995 are separated by 272 nucleotides CA265_RS14995 and CA265_RS15000 are separated by 115 nucleotides
CA265_RS14990: CA265_RS14990 - amino acid transporter, at 3,776,931 to 3,778,373
_RS14990
CA265_RS14995: CA265_RS14995 - sodium-translocating pyrophosphatase, at 3,778,646 to 3,780,892
_RS14995
CA265_RS15000: CA265_RS15000 - amino acid permease, at 3,781,008 to 3,782,483
_RS15000
Position (kb)
3778
3779
3780
3781 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 3777.678 kb on + strand, within CA265_RS14990 at 3777.740 kb on + strand, within CA265_RS14990 at 3777.749 kb on + strand, within CA265_RS14990 at 3777.832 kb on - strand, within CA265_RS14990 at 3777.903 kb on - strand, within CA265_RS14990 at 3777.904 kb on + strand, within CA265_RS14990 at 3778.006 kb on - strand, within CA265_RS14990 at 3778.032 kb on + strand, within CA265_RS14990 at 3778.039 kb on - strand, within CA265_RS14990 at 3778.049 kb on + strand, within CA265_RS14990 at 3778.126 kb on + strand, within CA265_RS14990 at 3778.131 kb on - strand, within CA265_RS14990 at 3778.133 kb on - strand, within CA265_RS14990 at 3778.133 kb on - strand, within CA265_RS14990 at 3778.228 kb on + strand, within CA265_RS14990 at 3778.269 kb on - strand at 3778.517 kb on + strand at 3778.574 kb on + strand at 3778.583 kb on - strand at 3778.615 kb on + strand at 3778.616 kb on - strand at 3778.619 kb on - strand at 3778.645 kb on + strand at 3778.711 kb on + strand at 3778.781 kb on + strand at 3778.784 kb on - strand at 3778.784 kb on - strand at 3778.809 kb on + strand at 3778.827 kb on + strand at 3778.827 kb on + strand at 3778.827 kb on + strand at 3778.827 kb on + strand at 3778.827 kb on + strand at 3778.922 kb on - strand, within CA265_RS14995 at 3778.928 kb on - strand, within CA265_RS14995 at 3778.936 kb on + strand, within CA265_RS14995 at 3778.941 kb on + strand, within CA265_RS14995 at 3778.942 kb on - strand, within CA265_RS14995 at 3778.950 kb on + strand, within CA265_RS14995 at 3778.950 kb on + strand, within CA265_RS14995 at 3779.006 kb on - strand, within CA265_RS14995 at 3779.006 kb on - strand, within CA265_RS14995 at 3779.083 kb on - strand, within CA265_RS14995 at 3779.083 kb on - strand, within CA265_RS14995 at 3779.088 kb on + strand, within CA265_RS14995 at 3779.125 kb on + strand, within CA265_RS14995 at 3779.126 kb on - strand, within CA265_RS14995 at 3779.146 kb on + strand, within CA265_RS14995 at 3779.155 kb on - strand, within CA265_RS14995 at 3779.258 kb on + strand, within CA265_RS14995 at 3779.259 kb on - strand, within CA265_RS14995 at 3779.296 kb on + strand, within CA265_RS14995 at 3779.328 kb on + strand, within CA265_RS14995 at 3779.328 kb on + strand, within CA265_RS14995 at 3779.329 kb on - strand, within CA265_RS14995 at 3779.345 kb on - strand, within CA265_RS14995 at 3779.366 kb on - strand, within CA265_RS14995 at 3779.411 kb on + strand, within CA265_RS14995 at 3779.412 kb on - strand, within CA265_RS14995 at 3779.461 kb on - strand, within CA265_RS14995 at 3779.521 kb on + strand, within CA265_RS14995 at 3779.526 kb on + strand at 3779.526 kb on + strand, within CA265_RS14995 at 3779.526 kb on + strand, within CA265_RS14995 at 3779.527 kb on - strand, within CA265_RS14995 at 3779.527 kb on - strand, within CA265_RS14995 at 3779.581 kb on - strand, within CA265_RS14995 at 3779.680 kb on - strand, within CA265_RS14995 at 3779.703 kb on + strand, within CA265_RS14995 at 3779.704 kb on - strand, within CA265_RS14995 at 3779.714 kb on + strand, within CA265_RS14995 at 3779.714 kb on + strand, within CA265_RS14995 at 3779.714 kb on + strand, within CA265_RS14995 at 3779.715 kb on - strand, within CA265_RS14995 at 3779.750 kb on - strand, within CA265_RS14995 at 3779.843 kb on - strand, within CA265_RS14995 at 3779.908 kb on + strand, within CA265_RS14995 at 3779.913 kb on + strand, within CA265_RS14995 at 3779.914 kb on - strand, within CA265_RS14995 at 3779.934 kb on + strand, within CA265_RS14995 at 3779.936 kb on + strand, within CA265_RS14995 at 3780.064 kb on - strand, within CA265_RS14995 at 3780.148 kb on - strand, within CA265_RS14995 at 3780.162 kb on + strand, within CA265_RS14995 at 3780.162 kb on + strand, within CA265_RS14995 at 3780.241 kb on - strand, within CA265_RS14995 at 3780.247 kb on + strand, within CA265_RS14995 at 3780.248 kb on - strand, within CA265_RS14995 at 3780.352 kb on + strand, within CA265_RS14995 at 3780.360 kb on - strand, within CA265_RS14995 at 3780.361 kb on + strand, within CA265_RS14995 at 3780.361 kb on + strand, within CA265_RS14995 at 3780.362 kb on - strand, within CA265_RS14995 at 3780.378 kb on - strand, within CA265_RS14995 at 3780.451 kb on + strand, within CA265_RS14995 at 3780.501 kb on + strand, within CA265_RS14995 at 3780.502 kb on - strand, within CA265_RS14995 at 3780.502 kb on - strand, within CA265_RS14995 at 3780.528 kb on + strand, within CA265_RS14995 at 3780.529 kb on - strand, within CA265_RS14995 at 3780.724 kb on - strand at 3780.745 kb on + strand at 3780.755 kb on + strand at 3780.756 kb on - strand at 3780.756 kb on - strand at 3780.838 kb on - strand at 3780.844 kb on + strand at 3780.909 kb on - strand at 3780.909 kb on - strand at 3780.909 kb on - strand at 3780.987 kb on - strand at 3781.004 kb on + strand at 3781.007 kb on + strand at 3781.040 kb on - strand at 3781.142 kb on - strand at 3781.216 kb on + strand, within CA265_RS15000 at 3781.217 kb on - strand, within CA265_RS15000 at 3781.237 kb on + strand, within CA265_RS15000 at 3781.238 kb on - strand, within CA265_RS15000 at 3781.285 kb on - strand, within CA265_RS15000 at 3781.287 kb on - strand, within CA265_RS15000 at 3781.287 kb on - strand, within CA265_RS15000 at 3781.287 kb on - strand, within CA265_RS15000 at 3781.290 kb on + strand, within CA265_RS15000 at 3781.292 kb on + strand, within CA265_RS15000 at 3781.296 kb on + strand, within CA265_RS15000 at 3781.316 kb on - strand, within CA265_RS15000 at 3781.354 kb on - strand, within CA265_RS15000 at 3781.370 kb on + strand, within CA265_RS15000 at 3781.375 kb on + strand, within CA265_RS15000 at 3781.376 kb on - strand, within CA265_RS15000 at 3781.376 kb on - strand, within CA265_RS15000 at 3781.408 kb on + strand, within CA265_RS15000 at 3781.409 kb on - strand, within CA265_RS15000 at 3781.423 kb on + strand, within CA265_RS15000 at 3781.424 kb on - strand, within CA265_RS15000 at 3781.427 kb on + strand, within CA265_RS15000 at 3781.427 kb on + strand, within CA265_RS15000 at 3781.428 kb on - strand, within CA265_RS15000 at 3781.490 kb on - strand, within CA265_RS15000 at 3781.618 kb on + strand, within CA265_RS15000 at 3781.689 kb on + strand, within CA265_RS15000 at 3781.689 kb on + strand, within CA265_RS15000 at 3781.812 kb on - strand, within CA265_RS15000 at 3781.866 kb on - strand, within CA265_RS15000 at 3781.873 kb on + strand, within CA265_RS15000 at 3781.873 kb on + strand, within CA265_RS15000 at 3781.874 kb on - strand, within CA265_RS15000 at 3781.875 kb on + strand, within CA265_RS15000 at 3781.876 kb on - strand, within CA265_RS15000 at 3781.877 kb on + strand, within CA265_RS15000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 25 mM remove 3,777,678 + CA265_RS14990 0.52 +2.0 3,777,740 + CA265_RS14990 0.56 +2.9 3,777,749 + CA265_RS14990 0.57 +3.7 3,777,832 - CA265_RS14990 0.62 +0.5 3,777,903 - CA265_RS14990 0.67 -0.5 3,777,904 + CA265_RS14990 0.67 +6.3 3,778,006 - CA265_RS14990 0.74 +0.2 3,778,032 + CA265_RS14990 0.76 +7.9 3,778,039 - CA265_RS14990 0.77 -0.1 3,778,049 + CA265_RS14990 0.77 +7.1 3,778,126 + CA265_RS14990 0.83 +9.4 3,778,131 - CA265_RS14990 0.83 +0.2 3,778,133 - CA265_RS14990 0.83 +0.3 3,778,133 - CA265_RS14990 0.83 +0.0 3,778,228 + CA265_RS14990 0.90 +9.5 3,778,269 - +1.0 3,778,517 + +8.9 3,778,574 + +8.9 3,778,583 - -2.9 3,778,615 + +8.8 3,778,616 - -2.3 3,778,619 - -3.5 3,778,645 + -0.5 3,778,711 + -2.6 3,778,781 + -3.5 3,778,784 - -2.9 3,778,784 - -2.1 3,778,809 + -2.8 3,778,827 + -2.4 3,778,827 + -3.1 3,778,827 + -0.7 3,778,827 + -1.8 3,778,827 + -2.7 3,778,922 - CA265_RS14995 0.12 -5.2 3,778,928 - CA265_RS14995 0.13 -4.4 3,778,936 + CA265_RS14995 0.13 -2.9 3,778,941 + CA265_RS14995 0.13 -2.3 3,778,942 - CA265_RS14995 0.13 -1.9 3,778,950 + CA265_RS14995 0.14 -0.4 3,778,950 + CA265_RS14995 0.14 -1.7 3,779,006 - CA265_RS14995 0.16 -2.4 3,779,006 - CA265_RS14995 0.16 -2.9 3,779,083 - CA265_RS14995 0.19 -1.9 3,779,083 - CA265_RS14995 0.19 -3.1 3,779,088 + CA265_RS14995 0.20 -3.7 3,779,125 + CA265_RS14995 0.21 -2.1 3,779,126 - CA265_RS14995 0.21 -3.8 3,779,146 + CA265_RS14995 0.22 -0.8 3,779,155 - CA265_RS14995 0.23 -3.5 3,779,258 + CA265_RS14995 0.27 -2.0 3,779,259 - CA265_RS14995 0.27 -3.7 3,779,296 + CA265_RS14995 0.29 -1.7 3,779,328 + CA265_RS14995 0.30 -2.5 3,779,328 + CA265_RS14995 0.30 -3.5 3,779,329 - CA265_RS14995 0.30 -4.8 3,779,345 - CA265_RS14995 0.31 -4.4 3,779,366 - CA265_RS14995 0.32 -2.9 3,779,411 + CA265_RS14995 0.34 -2.4 3,779,412 - CA265_RS14995 0.34 -2.1 3,779,461 - CA265_RS14995 0.36 -4.6 3,779,521 + CA265_RS14995 0.39 -4.0 3,779,526 + +0.6 3,779,526 + CA265_RS14995 0.39 -1.8 3,779,526 + CA265_RS14995 0.39 -4.5 3,779,527 - CA265_RS14995 0.39 -3.0 3,779,527 - CA265_RS14995 0.39 -3.1 3,779,581 - CA265_RS14995 0.42 -3.9 3,779,680 - CA265_RS14995 0.46 -3.3 3,779,703 + CA265_RS14995 0.47 -3.2 3,779,704 - CA265_RS14995 0.47 -5.2 3,779,714 + CA265_RS14995 0.48 -3.1 3,779,714 + CA265_RS14995 0.48 -2.6 3,779,714 + CA265_RS14995 0.48 -4.0 3,779,715 - CA265_RS14995 0.48 -2.0 3,779,750 - CA265_RS14995 0.49 -3.7 3,779,843 - CA265_RS14995 0.53 -3.0 3,779,908 + CA265_RS14995 0.56 -2.5 3,779,913 + CA265_RS14995 0.56 -2.0 3,779,914 - CA265_RS14995 0.56 -4.6 3,779,934 + CA265_RS14995 0.57 -2.5 3,779,936 + CA265_RS14995 0.57 -2.8 3,780,064 - CA265_RS14995 0.63 -2.4 3,780,148 - CA265_RS14995 0.67 -1.9 3,780,162 + CA265_RS14995 0.67 -1.2 3,780,162 + CA265_RS14995 0.67 -1.2 3,780,241 - CA265_RS14995 0.71 -1.9 3,780,247 + CA265_RS14995 0.71 -2.8 3,780,248 - CA265_RS14995 0.71 -3.5 3,780,352 + CA265_RS14995 0.76 -4.7 3,780,360 - CA265_RS14995 0.76 -3.9 3,780,361 + CA265_RS14995 0.76 -3.8 3,780,361 + CA265_RS14995 0.76 -3.8 3,780,362 - CA265_RS14995 0.76 -4.7 3,780,378 - CA265_RS14995 0.77 -1.7 3,780,451 + CA265_RS14995 0.80 -3.5 3,780,501 + CA265_RS14995 0.83 -2.6 3,780,502 - CA265_RS14995 0.83 -4.6 3,780,502 - CA265_RS14995 0.83 -2.1 3,780,528 + CA265_RS14995 0.84 -0.5 3,780,529 - CA265_RS14995 0.84 -1.5 3,780,724 - -3.4 3,780,745 + -2.6 3,780,755 + -2.2 3,780,756 - -1.4 3,780,756 - -3.1 3,780,838 - -2.7 3,780,844 + -0.9 3,780,909 - -2.0 3,780,909 - -2.1 3,780,909 - -2.2 3,780,987 - -0.8 3,781,004 + +1.0 3,781,007 + +0.4 3,781,040 - +0.4 3,781,142 - -0.6 3,781,216 + CA265_RS15000 0.14 +2.1 3,781,217 - CA265_RS15000 0.14 +2.6 3,781,237 + CA265_RS15000 0.16 +0.9 3,781,238 - CA265_RS15000 0.16 +0.9 3,781,285 - CA265_RS15000 0.19 -0.1 3,781,287 - CA265_RS15000 0.19 -0.1 3,781,287 - CA265_RS15000 0.19 +1.1 3,781,287 - CA265_RS15000 0.19 +0.6 3,781,290 + CA265_RS15000 0.19 +0.7 3,781,292 + CA265_RS15000 0.19 +1.9 3,781,296 + CA265_RS15000 0.20 +1.6 3,781,316 - CA265_RS15000 0.21 +0.4 3,781,354 - CA265_RS15000 0.23 +1.2 3,781,370 + CA265_RS15000 0.25 +1.5 3,781,375 + CA265_RS15000 0.25 +1.2 3,781,376 - CA265_RS15000 0.25 +0.6 3,781,376 - CA265_RS15000 0.25 +1.1 3,781,408 + CA265_RS15000 0.27 +2.0 3,781,409 - CA265_RS15000 0.27 +0.5 3,781,423 + CA265_RS15000 0.28 +1.0 3,781,424 - CA265_RS15000 0.28 +0.5 3,781,427 + CA265_RS15000 0.28 +0.6 3,781,427 + CA265_RS15000 0.28 +1.2 3,781,428 - CA265_RS15000 0.28 +0.9 3,781,490 - CA265_RS15000 0.33 +0.9 3,781,618 + CA265_RS15000 0.41 +0.6 3,781,689 + CA265_RS15000 0.46 +1.5 3,781,689 + CA265_RS15000 0.46 +0.3 3,781,812 - CA265_RS15000 0.54 +1.9 3,781,866 - CA265_RS15000 0.58 +1.0 3,781,873 + CA265_RS15000 0.59 +1.0 3,781,873 + CA265_RS15000 0.59 -2.0 3,781,874 - CA265_RS15000 0.59 +1.6 3,781,875 + CA265_RS15000 0.59 +0.4 3,781,876 - CA265_RS15000 0.59 +1.6 3,781,877 + CA265_RS15000 0.59 +1.2
Or see this region's nucleotide sequence