Experiment: R2A with sodium fluoride 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08810 and CA265_RS08815 are separated by 51 nucleotides CA265_RS08815 and CA265_RS08820 are separated by 53 nucleotides CA265_RS08820 and CA265_RS08825 overlap by 1 nucleotides
CA265_RS08810: CA265_RS08810 - MBL fold metallo-hydrolase, at 2,205,395 to 2,206,489
_RS08810
CA265_RS08815: CA265_RS08815 - hypothetical protein, at 2,206,541 to 2,206,903
_RS08815
CA265_RS08820: CA265_RS08820 - hypothetical protein, at 2,206,957 to 2,207,739
_RS08820
CA265_RS08825: CA265_RS08825 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 2,207,739 to 2,208,668
_RS08825
Position (kb)
2206
2207
2208 Strain fitness (log2 ratio)
-1
0
1
2 at 2205.980 kb on - strand, within CA265_RS08810 at 2206.045 kb on - strand, within CA265_RS08810 at 2206.117 kb on - strand, within CA265_RS08810 at 2206.136 kb on - strand, within CA265_RS08810 at 2206.212 kb on + strand, within CA265_RS08810 at 2206.235 kb on + strand, within CA265_RS08810 at 2206.262 kb on - strand, within CA265_RS08810 at 2206.493 kb on + strand at 2206.511 kb on + strand at 2206.511 kb on + strand at 2206.519 kb on - strand at 2206.596 kb on - strand, within CA265_RS08815 at 2206.612 kb on + strand, within CA265_RS08815 at 2206.613 kb on - strand, within CA265_RS08815 at 2206.665 kb on + strand, within CA265_RS08815 at 2206.671 kb on + strand, within CA265_RS08815 at 2206.718 kb on - strand, within CA265_RS08815 at 2206.718 kb on - strand, within CA265_RS08815 at 2206.732 kb on - strand, within CA265_RS08815 at 2206.732 kb on - strand, within CA265_RS08815 at 2206.748 kb on - strand, within CA265_RS08815 at 2206.775 kb on - strand, within CA265_RS08815 at 2206.780 kb on - strand, within CA265_RS08815 at 2206.857 kb on + strand, within CA265_RS08815 at 2206.874 kb on - strand at 2206.974 kb on + strand at 2207.001 kb on + strand at 2207.001 kb on + strand at 2207.002 kb on - strand at 2207.063 kb on + strand, within CA265_RS08820 at 2207.064 kb on - strand, within CA265_RS08820 at 2207.081 kb on + strand, within CA265_RS08820 at 2207.081 kb on + strand, within CA265_RS08820 at 2207.097 kb on - strand, within CA265_RS08820 at 2207.167 kb on - strand, within CA265_RS08820 at 2207.173 kb on - strand, within CA265_RS08820 at 2207.279 kb on + strand, within CA265_RS08820 at 2207.498 kb on + strand, within CA265_RS08820 at 2207.532 kb on + strand, within CA265_RS08820 at 2207.554 kb on + strand, within CA265_RS08820 at 2207.587 kb on + strand, within CA265_RS08820 at 2207.588 kb on - strand, within CA265_RS08820 at 2207.588 kb on - strand, within CA265_RS08820 at 2207.604 kb on + strand, within CA265_RS08820 at 2207.834 kb on + strand, within CA265_RS08825 at 2207.947 kb on + strand, within CA265_RS08825 at 2208.011 kb on + strand, within CA265_RS08825 at 2208.012 kb on - strand at 2208.060 kb on + strand, within CA265_RS08825 at 2208.060 kb on + strand, within CA265_RS08825 at 2208.061 kb on - strand, within CA265_RS08825 at 2208.069 kb on - strand at 2208.119 kb on - strand, within CA265_RS08825 at 2208.136 kb on + strand, within CA265_RS08825 at 2208.137 kb on - strand, within CA265_RS08825 at 2208.249 kb on + strand, within CA265_RS08825 at 2208.260 kb on + strand, within CA265_RS08825 at 2208.260 kb on + strand, within CA265_RS08825 at 2208.260 kb on + strand, within CA265_RS08825 at 2208.261 kb on - strand, within CA265_RS08825 at 2208.263 kb on + strand, within CA265_RS08825 at 2208.264 kb on - strand, within CA265_RS08825 at 2208.294 kb on - strand, within CA265_RS08825 at 2208.371 kb on + strand, within CA265_RS08825 at 2208.382 kb on + strand, within CA265_RS08825 at 2208.416 kb on + strand, within CA265_RS08825 at 2208.416 kb on + strand, within CA265_RS08825 at 2208.417 kb on - strand, within CA265_RS08825 at 2208.417 kb on - strand, within CA265_RS08825 at 2208.508 kb on + strand, within CA265_RS08825 at 2208.551 kb on + strand, within CA265_RS08825 at 2208.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with sodium fluoride 25 mM remove 2,205,980 - CA265_RS08810 0.53 -0.6 2,206,045 - CA265_RS08810 0.59 +0.2 2,206,117 - CA265_RS08810 0.66 -0.7 2,206,136 - CA265_RS08810 0.68 +0.0 2,206,212 + CA265_RS08810 0.75 +0.3 2,206,235 + CA265_RS08810 0.77 +0.6 2,206,262 - CA265_RS08810 0.79 +0.3 2,206,493 + -0.8 2,206,511 + -0.4 2,206,511 + +1.1 2,206,519 - +1.0 2,206,596 - CA265_RS08815 0.15 -0.6 2,206,612 + CA265_RS08815 0.20 +1.1 2,206,613 - CA265_RS08815 0.20 -0.3 2,206,665 + CA265_RS08815 0.34 +0.9 2,206,671 + CA265_RS08815 0.36 +0.0 2,206,718 - CA265_RS08815 0.49 +0.5 2,206,718 - CA265_RS08815 0.49 -0.4 2,206,732 - CA265_RS08815 0.53 -0.2 2,206,732 - CA265_RS08815 0.53 +0.2 2,206,748 - CA265_RS08815 0.57 -0.1 2,206,775 - CA265_RS08815 0.64 +0.2 2,206,780 - CA265_RS08815 0.66 -0.1 2,206,857 + CA265_RS08815 0.87 +0.7 2,206,874 - -0.7 2,206,974 + +0.3 2,207,001 + +0.0 2,207,001 + +0.4 2,207,002 - +0.9 2,207,063 + CA265_RS08820 0.14 +0.2 2,207,064 - CA265_RS08820 0.14 -0.3 2,207,081 + CA265_RS08820 0.16 +0.1 2,207,081 + CA265_RS08820 0.16 +1.6 2,207,097 - CA265_RS08820 0.18 +0.3 2,207,167 - CA265_RS08820 0.27 +0.6 2,207,173 - CA265_RS08820 0.28 +0.2 2,207,279 + CA265_RS08820 0.41 +0.5 2,207,498 + CA265_RS08820 0.69 +0.1 2,207,532 + CA265_RS08820 0.73 -0.1 2,207,554 + CA265_RS08820 0.76 +1.8 2,207,587 + CA265_RS08820 0.80 -0.4 2,207,588 - CA265_RS08820 0.81 +0.1 2,207,588 - CA265_RS08820 0.81 +0.8 2,207,604 + CA265_RS08820 0.83 +0.4 2,207,834 + CA265_RS08825 0.10 +1.4 2,207,947 + CA265_RS08825 0.22 +0.4 2,208,011 + CA265_RS08825 0.29 +0.7 2,208,012 - -0.1 2,208,060 + CA265_RS08825 0.35 -0.1 2,208,060 + CA265_RS08825 0.35 +0.4 2,208,061 - CA265_RS08825 0.35 +0.9 2,208,069 - -0.1 2,208,119 - CA265_RS08825 0.41 +0.8 2,208,136 + CA265_RS08825 0.43 +1.4 2,208,137 - CA265_RS08825 0.43 +0.9 2,208,249 + CA265_RS08825 0.55 +1.1 2,208,260 + CA265_RS08825 0.56 +0.3 2,208,260 + CA265_RS08825 0.56 +1.3 2,208,260 + CA265_RS08825 0.56 -0.0 2,208,261 - CA265_RS08825 0.56 +0.4 2,208,263 + CA265_RS08825 0.56 +1.4 2,208,264 - CA265_RS08825 0.56 +1.0 2,208,294 - CA265_RS08825 0.60 +1.0 2,208,371 + CA265_RS08825 0.68 +0.2 2,208,382 + CA265_RS08825 0.69 +0.3 2,208,416 + CA265_RS08825 0.73 +1.2 2,208,416 + CA265_RS08825 0.73 +0.3 2,208,417 - CA265_RS08825 0.73 +1.1 2,208,417 - CA265_RS08825 0.73 +0.5 2,208,508 + CA265_RS08825 0.83 +2.1 2,208,551 + CA265_RS08825 0.87 +0.4 2,208,673 + -0.2
Or see this region's nucleotide sequence