Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_749 and Pf1N1B4_750 overlap by 4 nucleotides Pf1N1B4_750 and Pf1N1B4_751 are separated by 178 nucleotides
Pf1N1B4_749 - hypothetical protein, at 852,642 to 852,824
_749
Pf1N1B4_750 - hypothetical protein, at 852,821 to 853,477
_750
Pf1N1B4_751 - hypothetical protein, at 853,656 to 854,096
_751
Position (kb) 853
854
855 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 852.794 kb on + strand, within Pf1N1B4_749 at 852.795 kb on - strand, within Pf1N1B4_749 at 852.795 kb on - strand, within Pf1N1B4_749 at 852.850 kb on + strand at 852.850 kb on + strand at 852.850 kb on + strand at 852.850 kb on + strand at 852.851 kb on - strand at 852.936 kb on + strand, within Pf1N1B4_750 at 852.936 kb on + strand, within Pf1N1B4_750 at 852.937 kb on - strand, within Pf1N1B4_750 at 853.188 kb on + strand, within Pf1N1B4_750 at 853.248 kb on + strand at 853.248 kb on + strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.249 kb on - strand, within Pf1N1B4_750 at 853.294 kb on + strand, within Pf1N1B4_750 at 853.294 kb on + strand, within Pf1N1B4_750 at 853.311 kb on - strand, within Pf1N1B4_750 at 853.494 kb on + strand at 853.494 kb on + strand at 853.495 kb on - strand at 853.495 kb on - strand at 853.529 kb on + strand at 853.530 kb on - strand at 853.530 kb on - strand at 853.598 kb on - strand at 853.818 kb on + strand, within Pf1N1B4_751 at 853.818 kb on + strand, within Pf1N1B4_751 at 853.819 kb on - strand, within Pf1N1B4_751 at 853.819 kb on - strand, within Pf1N1B4_751 at 854.083 kb on - strand at 854.213 kb on + strand at 854.300 kb on + strand at 854.300 kb on + strand at 854.361 kb on - strand at 854.421 kb on + strand at 854.422 kb on - strand at 854.422 kb on - strand at 854.434 kb on + strand at 854.435 kb on - strand at 854.435 kb on - strand at 854.438 kb on + strand at 854.547 kb on + strand at 854.547 kb on + strand at 854.598 kb on - strand at 854.656 kb on + strand at 854.657 kb on - strand at 854.801 kb on - strand at 854.864 kb on + strand at 854.865 kb on - strand at 854.915 kb on + strand at 854.958 kb on + strand at 854.959 kb on - strand at 854.959 kb on - strand at 854.959 kb on - strand at 854.998 kb on + strand at 854.999 kb on - strand at 854.999 kb on - strand at 855.002 kb on - strand at 855.002 kb on - strand at 855.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 852,794 + Pf1N1B4_749 0.83 +0.3 852,795 - Pf1N1B4_749 0.84 -0.0 852,795 - Pf1N1B4_749 0.84 +0.3 852,850 + -0.9 852,850 + -0.2 852,850 + +0.1 852,850 + +0.8 852,851 - +0.4 852,936 + Pf1N1B4_750 0.18 -0.2 852,936 + Pf1N1B4_750 0.18 -0.8 852,937 - Pf1N1B4_750 0.18 -0.8 853,188 + Pf1N1B4_750 0.56 +1.8 853,248 + +2.6 853,248 + Pf1N1B4_750 0.65 +2.0 853,249 - Pf1N1B4_750 0.65 +0.9 853,249 - Pf1N1B4_750 0.65 +0.9 853,249 - Pf1N1B4_750 0.65 +1.1 853,249 - Pf1N1B4_750 0.65 -0.2 853,249 - Pf1N1B4_750 0.65 -0.3 853,249 - Pf1N1B4_750 0.65 -0.2 853,249 - Pf1N1B4_750 0.65 -0.3 853,249 - Pf1N1B4_750 0.65 -0.4 853,294 + Pf1N1B4_750 0.72 -0.2 853,294 + Pf1N1B4_750 0.72 -0.0 853,311 - Pf1N1B4_750 0.75 -0.3 853,494 + +1.0 853,494 + +1.1 853,495 - -0.2 853,495 - +0.1 853,529 + -0.2 853,530 - -0.4 853,530 - -0.9 853,598 - -0.4 853,818 + Pf1N1B4_751 0.37 +5.4 853,818 + Pf1N1B4_751 0.37 +1.8 853,819 - Pf1N1B4_751 0.37 -0.5 853,819 - Pf1N1B4_751 0.37 -0.4 854,083 - -0.4 854,213 + -0.4 854,300 + -0.8 854,300 + -0.8 854,361 - -1.8 854,421 + +0.2 854,422 - -0.0 854,422 - -0.9 854,434 + +1.6 854,435 - +0.8 854,435 - -1.8 854,438 + -1.6 854,547 + -0.4 854,547 + -0.8 854,598 - +1.0 854,656 + -0.6 854,657 - +0.1 854,801 - +0.3 854,864 + +1.6 854,865 - +0.2 854,915 + +0.2 854,958 + -0.4 854,959 - -1.8 854,959 - +1.2 854,959 - -0.5 854,998 + -0.1 854,999 - +1.1 854,999 - +0.1 855,002 - +1.6 855,002 - +0.6 855,002 - -0.8
Or see this region's nucleotide sequence