Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5813 and Pf1N1B4_5814 are separated by 60 nucleotides Pf1N1B4_5814 and Pf1N1B4_5815 are separated by 15 nucleotides
Pf1N1B4_5813 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 245,881 to 246,756
_5813
Pf1N1B4_5814 - putative membrane protein, at 246,817 to 247,203
_5814
Pf1N1B4_5815 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 247,219 to 248,796
_5815
Position (kb) 246
247
248 Strain fitness (log2 ratio) -2
-1
0
1
2 at 245.834 kb on + strand at 245.834 kb on + strand at 245.834 kb on + strand at 245.834 kb on + strand at 245.834 kb on + strand at 245.835 kb on - strand at 245.898 kb on + strand at 245.899 kb on - strand at 245.899 kb on - strand at 245.899 kb on - strand at 246.197 kb on - strand, within Pf1N1B4_5813 at 246.199 kb on + strand, within Pf1N1B4_5813 at 246.199 kb on + strand, within Pf1N1B4_5813 at 246.458 kb on + strand, within Pf1N1B4_5813 at 246.582 kb on - strand, within Pf1N1B4_5813 at 246.755 kb on - strand at 246.797 kb on + strand at 247.057 kb on + strand, within Pf1N1B4_5814 at 247.057 kb on + strand, within Pf1N1B4_5814 at 247.058 kb on - strand, within Pf1N1B4_5814 at 247.058 kb on - strand, within Pf1N1B4_5814 at 247.147 kb on + strand, within Pf1N1B4_5814 at 247.148 kb on - strand, within Pf1N1B4_5814 at 247.148 kb on - strand, within Pf1N1B4_5814 at 247.265 kb on + strand at 247.265 kb on + strand at 247.266 kb on - strand at 247.734 kb on + strand, within Pf1N1B4_5815 at 247.785 kb on + strand, within Pf1N1B4_5815 at 247.788 kb on + strand, within Pf1N1B4_5815 at 247.789 kb on - strand, within Pf1N1B4_5815 at 248.186 kb on + strand, within Pf1N1B4_5815 at 248.186 kb on + strand at 248.187 kb on - strand, within Pf1N1B4_5815
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 245,834 + -1.6 245,834 + -0.4 245,834 + +0.9 245,834 + -0.8 245,834 + +0.0 245,835 - +1.5 245,898 + -2.3 245,899 - -0.5 245,899 - -0.4 245,899 - +0.2 246,197 - Pf1N1B4_5813 0.36 -0.6 246,199 + Pf1N1B4_5813 0.36 -0.8 246,199 + Pf1N1B4_5813 0.36 +0.8 246,458 + Pf1N1B4_5813 0.66 -0.1 246,582 - Pf1N1B4_5813 0.80 -0.1 246,755 - -1.0 246,797 + -1.1 247,057 + Pf1N1B4_5814 0.62 -0.4 247,057 + Pf1N1B4_5814 0.62 +0.8 247,058 - Pf1N1B4_5814 0.62 -1.8 247,058 - Pf1N1B4_5814 0.62 -2.0 247,147 + Pf1N1B4_5814 0.85 -1.8 247,148 - Pf1N1B4_5814 0.86 -1.8 247,148 - Pf1N1B4_5814 0.86 -0.0 247,265 + +0.3 247,265 + -1.8 247,266 - +0.2 247,734 + Pf1N1B4_5815 0.33 -0.8 247,785 + Pf1N1B4_5815 0.36 +1.0 247,788 + Pf1N1B4_5815 0.36 -0.9 247,789 - Pf1N1B4_5815 0.36 +1.2 248,186 + Pf1N1B4_5815 0.61 +0.2 248,186 + +1.8 248,187 - Pf1N1B4_5815 0.61 -1.0
Or see this region's nucleotide sequence