Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5808 and Pf1N1B4_5809 are separated by 169 nucleotides Pf1N1B4_5809 and Pf1N1B4_5810 are separated by 247 nucleotides Pf1N1B4_5810 and Pf1N1B4_5811 are separated by 67 nucleotides Pf1N1B4_5811 and Pf1N1B4_5812 overlap by 4 nucleotides
Pf1N1B4_5808 - Uricase (urate oxidase) (EC 1.7.3.3), at 241,988 to 242,887
_5808
Pf1N1B4_5809 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 243,057 to 244,004
_5809
Pf1N1B4_5810 - hypothetical protein, at 244,252 to 244,410
_5810
Pf1N1B4_5811 - FIG00958502: hypothetical protein, at 244,478 to 244,792
_5811
Pf1N1B4_5812 - FIG00955248: hypothetical protein, at 244,789 to 245,487
_5812
Position (kb) 243
244
245 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 242.103 kb on + strand, within Pf1N1B4_5808 at 242.104 kb on - strand, within Pf1N1B4_5808 at 242.166 kb on + strand, within Pf1N1B4_5808 at 242.167 kb on - strand, within Pf1N1B4_5808 at 242.216 kb on + strand, within Pf1N1B4_5808 at 242.296 kb on + strand, within Pf1N1B4_5808 at 242.369 kb on + strand at 242.369 kb on + strand, within Pf1N1B4_5808 at 242.370 kb on - strand, within Pf1N1B4_5808 at 242.377 kb on + strand, within Pf1N1B4_5808 at 242.378 kb on - strand, within Pf1N1B4_5808 at 242.378 kb on - strand, within Pf1N1B4_5808 at 242.405 kb on - strand, within Pf1N1B4_5808 at 242.415 kb on + strand, within Pf1N1B4_5808 at 243.411 kb on - strand at 243.454 kb on + strand, within Pf1N1B4_5809 at 243.541 kb on + strand, within Pf1N1B4_5809 at 243.610 kb on + strand, within Pf1N1B4_5809 at 243.610 kb on + strand, within Pf1N1B4_5809 at 243.630 kb on + strand, within Pf1N1B4_5809 at 243.630 kb on + strand, within Pf1N1B4_5809 at 243.630 kb on + strand, within Pf1N1B4_5809 at 243.844 kb on + strand, within Pf1N1B4_5809 at 243.895 kb on + strand, within Pf1N1B4_5809 at 243.898 kb on + strand, within Pf1N1B4_5809 at 243.940 kb on + strand at 243.988 kb on + strand at 244.144 kb on + strand at 244.176 kb on + strand at 244.176 kb on + strand at 244.177 kb on - strand at 244.222 kb on - strand at 244.454 kb on + strand at 244.454 kb on + strand at 244.454 kb on + strand at 244.455 kb on - strand at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.548 kb on + strand, within Pf1N1B4_5811 at 244.549 kb on - strand at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.549 kb on - strand, within Pf1N1B4_5811 at 244.733 kb on + strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.734 kb on - strand, within Pf1N1B4_5811 at 244.805 kb on + strand at 244.805 kb on + strand at 244.806 kb on - strand at 244.806 kb on - strand at 244.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 242,103 + Pf1N1B4_5808 0.13 -1.8 242,104 - Pf1N1B4_5808 0.13 -0.8 242,166 + Pf1N1B4_5808 0.20 -0.4 242,167 - Pf1N1B4_5808 0.20 -1.6 242,216 + Pf1N1B4_5808 0.25 -0.7 242,296 + Pf1N1B4_5808 0.34 -0.8 242,369 + -0.4 242,369 + Pf1N1B4_5808 0.42 -1.4 242,370 - Pf1N1B4_5808 0.42 -2.0 242,377 + Pf1N1B4_5808 0.43 +1.8 242,378 - Pf1N1B4_5808 0.43 +0.6 242,378 - Pf1N1B4_5808 0.43 -1.3 242,405 - Pf1N1B4_5808 0.46 +0.3 242,415 + Pf1N1B4_5808 0.47 -1.6 243,411 - -1.4 243,454 + Pf1N1B4_5809 0.42 -1.1 243,541 + Pf1N1B4_5809 0.51 -0.4 243,610 + Pf1N1B4_5809 0.58 -1.1 243,610 + Pf1N1B4_5809 0.58 -0.8 243,630 + Pf1N1B4_5809 0.60 -1.3 243,630 + Pf1N1B4_5809 0.60 -0.7 243,630 + Pf1N1B4_5809 0.60 -0.8 243,844 + Pf1N1B4_5809 0.83 -0.5 243,895 + Pf1N1B4_5809 0.88 -0.8 243,898 + Pf1N1B4_5809 0.89 +0.1 243,940 + -0.9 243,988 + +1.2 244,144 + -0.6 244,176 + -0.4 244,176 + -0.6 244,177 - -1.4 244,222 - -0.8 244,454 + -0.3 244,454 + +1.2 244,454 + +0.0 244,455 - +0.2 244,548 + Pf1N1B4_5811 0.22 -1.8 244,548 + Pf1N1B4_5811 0.22 +1.2 244,548 + Pf1N1B4_5811 0.22 -0.0 244,548 + Pf1N1B4_5811 0.22 -1.4 244,548 + Pf1N1B4_5811 0.22 -0.3 244,549 - -0.8 244,549 - Pf1N1B4_5811 0.23 -0.5 244,549 - Pf1N1B4_5811 0.23 -1.3 244,549 - Pf1N1B4_5811 0.23 +0.2 244,549 - Pf1N1B4_5811 0.23 +1.6 244,733 + Pf1N1B4_5811 0.81 +0.9 244,734 - Pf1N1B4_5811 0.81 +0.2 244,734 - Pf1N1B4_5811 0.81 -1.3 244,734 - Pf1N1B4_5811 0.81 -0.7 244,734 - Pf1N1B4_5811 0.81 +0.8 244,734 - Pf1N1B4_5811 0.81 -0.4 244,805 + +1.6 244,805 + +0.2 244,806 - -3.1 244,806 - -1.1 244,822 - -0.8
Or see this region's nucleotide sequence