Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5260 and Pf1N1B4_5261 are separated by 280 nucleotides Pf1N1B4_5261 and Pf1N1B4_5262 are separated by 372 nucleotides Pf1N1B4_5262 and Pf1N1B4_5263 are separated by 491 nucleotides Pf1N1B4_5263 and Pf1N1B4_5264 are separated by 10 nucleotides
Pf1N1B4_5260 - hypothetical protein, at 19,541 to 20,695
_5260
Pf1N1B4_5261 - hypothetical protein, at 20,976 to 21,173
_5261
Pf1N1B4_5262 - hypothetical protein, at 21,546 to 22,034
_5262
Pf1N1B4_5263 - hypothetical protein, at 22,526 to 22,723
_5263
Pf1N1B4_5264 - hypothetical protein, at 22,734 to 22,868
_5264
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 20.627 kb on + strand at 20.627 kb on + strand at 20.630 kb on + strand at 20.657 kb on + strand at 20.758 kb on + strand at 20.977 kb on + strand at 20.977 kb on + strand at 21.099 kb on + strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.100 kb on - strand, within Pf1N1B4_5261 at 21.200 kb on + strand at 21.220 kb on + strand at 21.359 kb on + strand at 21.360 kb on - strand at 21.589 kb on + strand at 21.724 kb on + strand, within Pf1N1B4_5262 at 21.725 kb on - strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.904 kb on + strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.905 kb on - strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.941 kb on + strand, within Pf1N1B4_5262 at 21.943 kb on + strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.944 kb on - strand, within Pf1N1B4_5262 at 21.961 kb on + strand, within Pf1N1B4_5262 at 22.078 kb on - strand at 22.104 kb on + strand at 22.105 kb on - strand at 22.105 kb on - strand at 22.159 kb on + strand at 22.159 kb on + strand at 22.160 kb on - strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.162 kb on + strand at 22.527 kb on + strand at 22.528 kb on - strand at 22.541 kb on + strand at 22.541 kb on + strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.542 kb on - strand at 22.604 kb on + strand, within Pf1N1B4_5263 at 22.604 kb on + strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.605 kb on - strand, within Pf1N1B4_5263 at 22.670 kb on + strand, within Pf1N1B4_5263 at 22.671 kb on - strand, within Pf1N1B4_5263 at 22.671 kb on - strand at 22.671 kb on - strand at 22.671 kb on - strand, within Pf1N1B4_5263 at 22.718 kb on - strand at 22.718 kb on - strand at 22.797 kb on + strand, within Pf1N1B4_5264 at 22.798 kb on - strand at 22.878 kb on + strand at 22.879 kb on - strand at 22.879 kb on - strand at 22.905 kb on - strand at 22.906 kb on + strand at 22.907 kb on - strand at 22.907 kb on - strand at 22.938 kb on - strand at 22.958 kb on - strand at 22.974 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 20,627 + -0.2 20,627 + -0.4 20,630 + +0.0 20,657 + +0.2 20,758 + -0.8 20,977 + +0.8 20,977 + -1.6 21,099 + Pf1N1B4_5261 0.62 -0.1 21,100 - Pf1N1B4_5261 0.63 -0.0 21,100 - Pf1N1B4_5261 0.63 -0.1 21,100 - Pf1N1B4_5261 0.63 +0.4 21,200 + -1.6 21,220 + -0.6 21,359 + +0.8 21,360 - -1.6 21,589 + +0.2 21,724 + Pf1N1B4_5262 0.36 -0.8 21,725 - Pf1N1B4_5262 0.37 +0.3 21,904 + Pf1N1B4_5262 0.73 +0.1 21,904 + Pf1N1B4_5262 0.73 -1.6 21,904 + Pf1N1B4_5262 0.73 -0.1 21,905 - Pf1N1B4_5262 0.73 -0.8 21,905 - Pf1N1B4_5262 0.73 -0.1 21,905 - Pf1N1B4_5262 0.73 +0.5 21,905 - Pf1N1B4_5262 0.73 +1.8 21,941 + Pf1N1B4_5262 0.81 +0.8 21,941 + Pf1N1B4_5262 0.81 +1.2 21,943 + Pf1N1B4_5262 0.81 -0.8 21,944 - Pf1N1B4_5262 0.81 -0.1 21,944 - Pf1N1B4_5262 0.81 +0.2 21,961 + Pf1N1B4_5262 0.85 +2.5 22,078 - +0.6 22,104 + -0.6 22,105 - +1.9 22,105 - +0.3 22,159 + -0.2 22,159 + +0.2 22,160 - -0.5 22,162 + -0.7 22,162 + -0.1 22,162 + -0.8 22,162 + -0.6 22,162 + +1.0 22,527 + +1.5 22,528 - +0.2 22,541 + -1.1 22,541 + -0.6 22,542 - +0.8 22,542 - +0.2 22,542 - -1.2 22,542 - -0.6 22,604 + Pf1N1B4_5263 0.39 -2.8 22,604 + Pf1N1B4_5263 0.39 -0.0 22,605 - Pf1N1B4_5263 0.40 +0.0 22,605 - Pf1N1B4_5263 0.40 -1.4 22,605 - Pf1N1B4_5263 0.40 +0.8 22,605 - Pf1N1B4_5263 0.40 +0.2 22,605 - Pf1N1B4_5263 0.40 +1.0 22,605 - Pf1N1B4_5263 0.40 -0.5 22,605 - Pf1N1B4_5263 0.40 +0.8 22,605 - Pf1N1B4_5263 0.40 -0.7 22,605 - Pf1N1B4_5263 0.40 +0.8 22,605 - Pf1N1B4_5263 0.40 -1.4 22,670 + Pf1N1B4_5263 0.73 +0.9 22,671 - Pf1N1B4_5263 0.73 +1.3 22,671 - +2.3 22,671 - +1.2 22,671 - Pf1N1B4_5263 0.73 -0.5 22,718 - +0.2 22,718 - +0.2 22,797 + Pf1N1B4_5264 0.47 -0.8 22,798 - +1.2 22,878 + +0.4 22,879 - -1.6 22,879 - +2.3 22,905 - +0.3 22,906 + +0.4 22,907 - -1.6 22,907 - -1.1 22,938 - -0.2 22,958 - +0.5 22,974 - +0.8
Or see this region's nucleotide sequence