Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5060 and Pf1N1B4_5061 overlap by 4 nucleotides Pf1N1B4_5061 and Pf1N1B4_5062 are separated by 259 nucleotides Pf1N1B4_5062 and Pf1N1B4_5063 are separated by 36 nucleotides Pf1N1B4_5063 and Pf1N1B4_5064 are separated by 96 nucleotides
Pf1N1B4_5060 - Ferrichrome-iron receptor, at 5,604,935 to 5,607,097
_5060
Pf1N1B4_5061 - Valyl-tRNA synthetase, at 5,607,094 to 5,607,402
_5061
Pf1N1B4_5062 - Homospermidine synthase (EC 2.5.1.44), at 5,607,662 to 5,609,086
_5062
Pf1N1B4_5063 - Phosphoribosyl transferase domain protein, at 5,609,123 to 5,609,812
_5063
Pf1N1B4_5064 - Universal stress protein family 5, at 5,609,909 to 5,610,847
_5064
Position (kb) 5607
5608
5609
5610 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5606.753 kb on + strand, within Pf1N1B4_5060 at 5606.753 kb on + strand, within Pf1N1B4_5060 at 5606.754 kb on - strand, within Pf1N1B4_5060 at 5606.909 kb on + strand at 5606.937 kb on - strand at 5607.026 kb on + strand at 5607.026 kb on + strand at 5607.026 kb on + strand at 5607.027 kb on - strand at 5607.027 kb on - strand at 5607.027 kb on - strand at 5607.134 kb on + strand, within Pf1N1B4_5061 at 5607.135 kb on - strand, within Pf1N1B4_5061 at 5607.147 kb on + strand, within Pf1N1B4_5061 at 5607.179 kb on + strand, within Pf1N1B4_5061 at 5607.179 kb on + strand, within Pf1N1B4_5061 at 5607.179 kb on + strand, within Pf1N1B4_5061 at 5607.180 kb on - strand, within Pf1N1B4_5061 at 5607.180 kb on - strand, within Pf1N1B4_5061 at 5607.876 kb on - strand, within Pf1N1B4_5062 at 5608.024 kb on + strand at 5608.025 kb on - strand, within Pf1N1B4_5062 at 5608.062 kb on - strand, within Pf1N1B4_5062 at 5608.062 kb on - strand, within Pf1N1B4_5062 at 5608.062 kb on - strand, within Pf1N1B4_5062 at 5608.431 kb on - strand, within Pf1N1B4_5062 at 5608.434 kb on - strand, within Pf1N1B4_5062 at 5608.463 kb on + strand, within Pf1N1B4_5062 at 5608.463 kb on + strand at 5608.464 kb on - strand at 5608.464 kb on - strand, within Pf1N1B4_5062 at 5608.572 kb on - strand, within Pf1N1B4_5062 at 5608.779 kb on - strand at 5608.785 kb on - strand, within Pf1N1B4_5062 at 5608.978 kb on - strand at 5608.978 kb on - strand at 5608.989 kb on - strand at 5608.989 kb on - strand at 5609.233 kb on - strand, within Pf1N1B4_5063 at 5609.474 kb on + strand, within Pf1N1B4_5063 at 5609.474 kb on + strand, within Pf1N1B4_5063 at 5609.475 kb on - strand, within Pf1N1B4_5063 at 5609.688 kb on + strand, within Pf1N1B4_5063 at 5609.689 kb on - strand, within Pf1N1B4_5063 at 5609.689 kb on - strand at 5609.744 kb on - strand at 5609.821 kb on + strand at 5609.948 kb on - strand at 5609.948 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 5,606,753 + Pf1N1B4_5060 0.84 -0.2 5,606,753 + Pf1N1B4_5060 0.84 +0.4 5,606,754 - Pf1N1B4_5060 0.84 +0.1 5,606,909 + +3.1 5,606,937 - -0.9 5,607,026 + -1.9 5,607,026 + +0.3 5,607,026 + -0.9 5,607,027 - -0.2 5,607,027 - +0.5 5,607,027 - +0.4 5,607,134 + Pf1N1B4_5061 0.13 +0.3 5,607,135 - Pf1N1B4_5061 0.13 -0.4 5,607,147 + Pf1N1B4_5061 0.17 -0.4 5,607,179 + Pf1N1B4_5061 0.28 -2.1 5,607,179 + Pf1N1B4_5061 0.28 +0.1 5,607,179 + Pf1N1B4_5061 0.28 +0.1 5,607,180 - Pf1N1B4_5061 0.28 +0.7 5,607,180 - Pf1N1B4_5061 0.28 +0.3 5,607,876 - Pf1N1B4_5062 0.15 -0.2 5,608,024 + +0.1 5,608,025 - Pf1N1B4_5062 0.25 -1.2 5,608,062 - Pf1N1B4_5062 0.28 -1.4 5,608,062 - Pf1N1B4_5062 0.28 -0.7 5,608,062 - Pf1N1B4_5062 0.28 +1.7 5,608,431 - Pf1N1B4_5062 0.54 -0.8 5,608,434 - Pf1N1B4_5062 0.54 +1.3 5,608,463 + Pf1N1B4_5062 0.56 -0.2 5,608,463 + +0.1 5,608,464 - -1.2 5,608,464 - Pf1N1B4_5062 0.56 +0.1 5,608,572 - Pf1N1B4_5062 0.64 -0.9 5,608,779 - -0.2 5,608,785 - Pf1N1B4_5062 0.79 +0.7 5,608,978 - -0.8 5,608,978 - -0.9 5,608,989 - -0.1 5,608,989 - -0.5 5,609,233 - Pf1N1B4_5063 0.16 +0.3 5,609,474 + Pf1N1B4_5063 0.51 +0.4 5,609,474 + Pf1N1B4_5063 0.51 +1.6 5,609,475 - Pf1N1B4_5063 0.51 -0.7 5,609,688 + Pf1N1B4_5063 0.82 -1.2 5,609,689 - Pf1N1B4_5063 0.82 -1.5 5,609,689 - +0.1 5,609,744 - -0.9 5,609,821 + -0.7 5,609,948 - +1.7 5,609,948 - -0.7
Or see this region's nucleotide sequence