Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4726 and Pf1N1B4_4727 overlap by 4 nucleotides Pf1N1B4_4727 and Pf1N1B4_4728 are separated by 115 nucleotides Pf1N1B4_4728 and Pf1N1B4_4729 are separated by 77 nucleotides
Pf1N1B4_4726 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 5,222,158 to 5,223,681
_4726
Pf1N1B4_4727 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 5,223,678 to 5,224,499
_4727
Pf1N1B4_4728 - Na+/H+ antiporter NhaB, at 5,224,615 to 5,226,117
_4728
Pf1N1B4_4729 - Hemolysin-type calcium-binding region, at 5,226,195 to 5,227,484
_4729
Position (kb) 5224
5225
5226
5227 Strain fitness (log2 ratio) -1
0
1
2
3 at 5223.910 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.942 kb on + strand, within Pf1N1B4_4727 at 5223.943 kb on - strand, within Pf1N1B4_4727 at 5224.119 kb on + strand at 5224.242 kb on + strand, within Pf1N1B4_4727 at 5224.242 kb on + strand, within Pf1N1B4_4727 at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.482 kb on + strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.483 kb on - strand at 5224.617 kb on - strand at 5224.617 kb on - strand at 5224.619 kb on - strand at 5224.626 kb on - strand at 5224.638 kb on - strand at 5224.665 kb on + strand at 5224.666 kb on - strand at 5224.666 kb on - strand at 5224.668 kb on - strand at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.875 kb on - strand, within Pf1N1B4_4728 at 5224.930 kb on - strand, within Pf1N1B4_4728 at 5225.630 kb on + strand, within Pf1N1B4_4728 at 5225.631 kb on - strand, within Pf1N1B4_4728 at 5225.796 kb on - strand, within Pf1N1B4_4728 at 5225.796 kb on - strand, within Pf1N1B4_4728 at 5225.796 kb on - strand, within Pf1N1B4_4728 at 5225.812 kb on - strand, within Pf1N1B4_4728 at 5225.859 kb on - strand, within Pf1N1B4_4728 at 5226.132 kb on - strand at 5226.135 kb on - strand at 5226.135 kb on - strand at 5226.252 kb on + strand at 5226.496 kb on - strand, within Pf1N1B4_4729 at 5226.496 kb on - strand at 5226.496 kb on - strand, within Pf1N1B4_4729 at 5226.496 kb on - strand, within Pf1N1B4_4729 at 5226.539 kb on + strand, within Pf1N1B4_4729 at 5226.852 kb on - strand at 5226.882 kb on + strand, within Pf1N1B4_4729
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 5,223,910 + Pf1N1B4_4727 0.28 +0.1 5,223,942 + Pf1N1B4_4727 0.32 +0.9 5,223,942 + Pf1N1B4_4727 0.32 -1.2 5,223,942 + Pf1N1B4_4727 0.32 +1.1 5,223,942 + Pf1N1B4_4727 0.32 -0.7 5,223,943 - Pf1N1B4_4727 0.32 -0.7 5,224,119 + -0.2 5,224,242 + Pf1N1B4_4727 0.69 -1.5 5,224,242 + Pf1N1B4_4727 0.69 -0.4 5,224,482 + -0.9 5,224,482 + +1.1 5,224,482 + -0.2 5,224,482 + +1.1 5,224,483 - +0.1 5,224,483 - -0.2 5,224,483 - -0.9 5,224,483 - -0.7 5,224,483 - +0.8 5,224,483 - +0.4 5,224,617 - -1.5 5,224,617 - -0.9 5,224,619 - +0.5 5,224,626 - -0.2 5,224,638 - +1.1 5,224,665 + +0.1 5,224,666 - -0.9 5,224,666 - +0.1 5,224,668 - -0.1 5,224,875 - Pf1N1B4_4728 0.17 +1.1 5,224,875 - Pf1N1B4_4728 0.17 +0.8 5,224,875 - Pf1N1B4_4728 0.17 +0.2 5,224,875 - Pf1N1B4_4728 0.17 +0.7 5,224,930 - Pf1N1B4_4728 0.21 -0.5 5,225,630 + Pf1N1B4_4728 0.68 +0.3 5,225,631 - Pf1N1B4_4728 0.68 +1.1 5,225,796 - Pf1N1B4_4728 0.79 +0.5 5,225,796 - Pf1N1B4_4728 0.79 +3.1 5,225,796 - Pf1N1B4_4728 0.79 -1.5 5,225,812 - Pf1N1B4_4728 0.80 +0.6 5,225,859 - Pf1N1B4_4728 0.83 +0.3 5,226,132 - -0.1 5,226,135 - +0.5 5,226,135 - -0.7 5,226,252 + -1.2 5,226,496 - Pf1N1B4_4729 0.23 -1.5 5,226,496 - +0.1 5,226,496 - Pf1N1B4_4729 0.23 -1.1 5,226,496 - Pf1N1B4_4729 0.23 +0.9 5,226,539 + Pf1N1B4_4729 0.27 -0.5 5,226,852 - -0.2 5,226,882 + Pf1N1B4_4729 0.53 -0.1
Or see this region's nucleotide sequence