Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4721 and Pf1N1B4_4722 are separated by 116 nucleotides Pf1N1B4_4722 and Pf1N1B4_4723 are separated by 194 nucleotides Pf1N1B4_4723 and Pf1N1B4_4724 are separated by 198 nucleotides Pf1N1B4_4724 and Pf1N1B4_4725 are separated by 272 nucleotides
Pf1N1B4_4721 - Nuclease inhibitor, at 5,217,398 to 5,217,979
_4721
Pf1N1B4_4722 - Histone acetyltransferase HPA2 and related acetyltransferases, at 5,218,096 to 5,218,587
_4722
Pf1N1B4_4723 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 5,218,782 to 5,220,527
_4723
Pf1N1B4_4724 - FIG00961385: hypothetical protein, at 5,220,726 to 5,220,908
_4724
Pf1N1B4_4725 - 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44), at 5,221,181 to 5,222,161
_4725
Position (kb)
5218
5219
5220
5221 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5218.132 kb on + strand at 5218.169 kb on + strand at 5218.224 kb on + strand, within Pf1N1B4_4722 at 5218.489 kb on - strand, within Pf1N1B4_4722 at 5218.698 kb on + strand at 5218.751 kb on + strand at 5218.751 kb on + strand at 5218.783 kb on + strand at 5218.809 kb on - strand at 5218.868 kb on - strand at 5219.164 kb on + strand, within Pf1N1B4_4723 at 5219.165 kb on - strand at 5219.233 kb on + strand, within Pf1N1B4_4723 at 5219.348 kb on - strand at 5219.761 kb on + strand, within Pf1N1B4_4723 at 5220.085 kb on + strand, within Pf1N1B4_4723 at 5220.178 kb on + strand, within Pf1N1B4_4723 at 5220.178 kb on + strand at 5220.405 kb on - strand at 5220.550 kb on - strand at 5220.550 kb on - strand at 5220.550 kb on - strand at 5220.550 kb on - strand at 5220.550 kb on - strand at 5220.550 kb on - strand at 5220.637 kb on - strand at 5220.637 kb on - strand at 5220.637 kb on - strand at 5220.898 kb on - strand at 5220.940 kb on - strand at 5220.970 kb on - strand at 5221.137 kb on + strand at 5221.137 kb on + strand at 5221.137 kb on + strand at 5221.157 kb on - strand at 5221.162 kb on + strand at 5221.166 kb on - strand at 5221.178 kb on + strand at 5221.178 kb on + strand at 5221.178 kb on + strand at 5221.178 kb on + strand at 5221.179 kb on - strand at 5221.179 kb on - strand at 5221.222 kb on + strand at 5221.252 kb on + strand at 5221.253 kb on - strand at 5221.265 kb on + strand at 5221.423 kb on + strand, within Pf1N1B4_4725 at 5221.423 kb on + strand, within Pf1N1B4_4725 at 5221.424 kb on - strand, within Pf1N1B4_4725 at 5221.472 kb on + strand, within Pf1N1B4_4725 at 5221.472 kb on + strand, within Pf1N1B4_4725 at 5221.472 kb on + strand, within Pf1N1B4_4725 at 5221.473 kb on - strand at 5221.473 kb on - strand, within Pf1N1B4_4725 at 5221.475 kb on - strand, within Pf1N1B4_4725
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 5,218,132 + +0.1 5,218,169 + -0.2 5,218,224 + Pf1N1B4_4722 0.26 -0.5 5,218,489 - Pf1N1B4_4722 0.80 -0.5 5,218,698 + -2.0 5,218,751 + -0.5 5,218,751 + -0.8 5,218,783 + -2.1 5,218,809 - +1.1 5,218,868 - -1.6 5,219,164 + Pf1N1B4_4723 0.22 -2.6 5,219,165 - +0.1 5,219,233 + Pf1N1B4_4723 0.26 -0.4 5,219,348 - -0.5 5,219,761 + Pf1N1B4_4723 0.56 +0.9 5,220,085 + Pf1N1B4_4723 0.75 +0.1 5,220,178 + Pf1N1B4_4723 0.80 +0.2 5,220,178 + -1.5 5,220,405 - +1.1 5,220,550 - -0.5 5,220,550 - +0.2 5,220,550 - -0.5 5,220,550 - +0.4 5,220,550 - -0.9 5,220,550 - -0.5 5,220,637 - -1.9 5,220,637 - -0.3 5,220,637 - +0.4 5,220,898 - -0.7 5,220,940 - -1.2 5,220,970 - -0.9 5,221,137 + -0.5 5,221,137 + -2.1 5,221,137 + -0.9 5,221,157 - -1.5 5,221,162 + +0.1 5,221,166 - -1.7 5,221,178 + -1.1 5,221,178 + -2.5 5,221,178 + +2.7 5,221,178 + -0.3 5,221,179 - -1.1 5,221,179 - -1.5 5,221,222 + -0.5 5,221,252 + -0.9 5,221,253 - +0.4 5,221,265 + -1.3 5,221,423 + Pf1N1B4_4725 0.25 -1.7 5,221,423 + Pf1N1B4_4725 0.25 -0.5 5,221,424 - Pf1N1B4_4725 0.25 -1.5 5,221,472 + Pf1N1B4_4725 0.30 +0.2 5,221,472 + Pf1N1B4_4725 0.30 -0.5 5,221,472 + Pf1N1B4_4725 0.30 -0.9 5,221,473 - +2.7 5,221,473 - Pf1N1B4_4725 0.30 -0.2 5,221,475 - Pf1N1B4_4725 0.30 -2.6
Or see this region's nucleotide sequence