Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_457 and Pf1N1B4_458 overlap by 1 nucleotides Pf1N1B4_458 and Pf1N1B4_459 overlap by 14 nucleotides
Pf1N1B4_457 - Periplasmic serine proteases (ClpP class), at 547,128 to 548,144
_457
Pf1N1B4_458 - Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C, at 548,144 to 548,806
_458
Pf1N1B4_459 - Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70), at 548,793 to 549,755
_459
Position (kb) 548
549 Strain fitness (log2 ratio) -2
-1
0
1 at 547.404 kb on - strand, within Pf1N1B4_457 at 547.513 kb on + strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.514 kb on - strand, within Pf1N1B4_457 at 547.518 kb on - strand, within Pf1N1B4_457 at 547.612 kb on + strand, within Pf1N1B4_457 at 547.613 kb on - strand, within Pf1N1B4_457 at 547.639 kb on + strand at 547.639 kb on + strand, within Pf1N1B4_457 at 547.639 kb on + strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.640 kb on - strand, within Pf1N1B4_457 at 547.642 kb on + strand, within Pf1N1B4_457 at 547.642 kb on + strand at 547.643 kb on - strand, within Pf1N1B4_457 at 547.688 kb on + strand at 547.689 kb on - strand, within Pf1N1B4_457 at 547.733 kb on - strand, within Pf1N1B4_457 at 547.873 kb on - strand, within Pf1N1B4_457 at 547.873 kb on - strand, within Pf1N1B4_457 at 547.873 kb on - strand, within Pf1N1B4_457 at 547.874 kb on + strand, within Pf1N1B4_457 at 547.875 kb on - strand, within Pf1N1B4_457 at 547.927 kb on - strand, within Pf1N1B4_457 at 547.927 kb on - strand, within Pf1N1B4_457 at 547.964 kb on - strand, within Pf1N1B4_457 at 548.050 kb on - strand at 548.122 kb on + strand at 548.122 kb on + strand at 548.123 kb on - strand at 548.123 kb on - strand at 548.123 kb on - strand at 548.125 kb on - strand at 548.125 kb on - strand at 548.125 kb on - strand at 548.125 kb on - strand at 548.146 kb on - strand at 548.241 kb on + strand at 548.241 kb on + strand, within Pf1N1B4_458 at 548.416 kb on - strand, within Pf1N1B4_458 at 548.416 kb on - strand, within Pf1N1B4_458 at 548.416 kb on - strand, within Pf1N1B4_458 at 548.416 kb on - strand, within Pf1N1B4_458 at 548.568 kb on + strand, within Pf1N1B4_458 at 548.568 kb on + strand, within Pf1N1B4_458 at 548.569 kb on - strand, within Pf1N1B4_458 at 548.569 kb on - strand, within Pf1N1B4_458 at 548.569 kb on - strand, within Pf1N1B4_458 at 548.598 kb on - strand, within Pf1N1B4_458 at 548.791 kb on - strand at 548.791 kb on - strand at 548.791 kb on - strand at 548.791 kb on - strand at 548.791 kb on - strand at 548.897 kb on - strand, within Pf1N1B4_459 at 549.002 kb on + strand, within Pf1N1B4_459 at 549.223 kb on + strand, within Pf1N1B4_459 at 549.460 kb on + strand, within Pf1N1B4_459 at 549.683 kb on + strand at 549.697 kb on + strand at 549.795 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 547,404 - Pf1N1B4_457 0.27 +0.5 547,513 + Pf1N1B4_457 0.38 +0.1 547,514 - Pf1N1B4_457 0.38 +1.5 547,514 - Pf1N1B4_457 0.38 -0.5 547,514 - Pf1N1B4_457 0.38 -0.8 547,514 - Pf1N1B4_457 0.38 -0.1 547,514 - Pf1N1B4_457 0.38 -1.8 547,514 - Pf1N1B4_457 0.38 -0.1 547,518 - Pf1N1B4_457 0.38 -1.1 547,612 + Pf1N1B4_457 0.48 +0.2 547,613 - Pf1N1B4_457 0.48 +1.5 547,639 + -0.1 547,639 + Pf1N1B4_457 0.50 +0.5 547,639 + Pf1N1B4_457 0.50 -0.5 547,640 - Pf1N1B4_457 0.50 -0.1 547,640 - Pf1N1B4_457 0.50 -0.3 547,640 - Pf1N1B4_457 0.50 -0.8 547,640 - Pf1N1B4_457 0.50 -0.5 547,640 - Pf1N1B4_457 0.50 -1.0 547,640 - Pf1N1B4_457 0.50 +0.5 547,640 - Pf1N1B4_457 0.50 -1.5 547,640 - Pf1N1B4_457 0.50 +1.1 547,640 - Pf1N1B4_457 0.50 -0.5 547,640 - Pf1N1B4_457 0.50 -1.8 547,642 + Pf1N1B4_457 0.51 -0.1 547,642 + -0.8 547,643 - Pf1N1B4_457 0.51 +0.8 547,688 + -0.1 547,689 - Pf1N1B4_457 0.55 -2.4 547,733 - Pf1N1B4_457 0.59 +0.7 547,873 - Pf1N1B4_457 0.73 -0.8 547,873 - Pf1N1B4_457 0.73 -1.1 547,873 - Pf1N1B4_457 0.73 -0.8 547,874 + Pf1N1B4_457 0.73 -0.1 547,875 - Pf1N1B4_457 0.73 -1.7 547,927 - Pf1N1B4_457 0.79 -0.1 547,927 - Pf1N1B4_457 0.79 -0.4 547,964 - Pf1N1B4_457 0.82 +0.1 548,050 - -0.5 548,122 + -0.3 548,122 + -1.1 548,123 - +0.6 548,123 - -1.1 548,123 - +0.8 548,125 - -0.5 548,125 - -1.5 548,125 - +0.6 548,125 - +0.0 548,146 - -0.5 548,241 + +0.5 548,241 + Pf1N1B4_458 0.15 -0.7 548,416 - Pf1N1B4_458 0.41 -0.3 548,416 - Pf1N1B4_458 0.41 -1.8 548,416 - Pf1N1B4_458 0.41 +0.0 548,416 - Pf1N1B4_458 0.41 -1.3 548,568 + Pf1N1B4_458 0.64 -0.5 548,568 + Pf1N1B4_458 0.64 -1.5 548,569 - Pf1N1B4_458 0.64 -1.1 548,569 - Pf1N1B4_458 0.64 -0.8 548,569 - Pf1N1B4_458 0.64 +1.1 548,598 - Pf1N1B4_458 0.68 -2.3 548,791 - +0.5 548,791 - +0.4 548,791 - -0.3 548,791 - +0.2 548,791 - -0.4 548,897 - Pf1N1B4_459 0.11 +0.2 549,002 + Pf1N1B4_459 0.22 -0.1 549,223 + Pf1N1B4_459 0.45 -0.1 549,460 + Pf1N1B4_459 0.69 -1.3 549,683 + -2.0 549,697 + -2.1 549,795 + -2.0
Or see this region's nucleotide sequence