Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4476 and Pf1N1B4_4477 are separated by 234 nucleotides Pf1N1B4_4477 and Pf1N1B4_4478 are separated by 18 nucleotides
Pf1N1B4_4476 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 4,936,524 to 4,937,543
_4476
Pf1N1B4_4477 - Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4), at 4,937,778 to 4,939,160
_4477
Pf1N1B4_4478 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 4,939,179 to 4,940,450
_4478
Position (kb)
4937
4938
4939
4940 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4936.918 kb on + strand, within Pf1N1B4_4476 at 4936.919 kb on - strand, within Pf1N1B4_4476 at 4936.978 kb on + strand, within Pf1N1B4_4476 at 4936.978 kb on + strand, within Pf1N1B4_4476 at 4936.979 kb on - strand, within Pf1N1B4_4476 at 4936.979 kb on - strand, within Pf1N1B4_4476 at 4936.979 kb on - strand, within Pf1N1B4_4476 at 4937.387 kb on - strand, within Pf1N1B4_4476 at 4937.500 kb on + strand at 4937.501 kb on - strand at 4937.554 kb on - strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.616 kb on + strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.617 kb on - strand at 4937.780 kb on - strand at 4938.254 kb on - strand, within Pf1N1B4_4477 at 4938.431 kb on + strand, within Pf1N1B4_4477 at 4938.431 kb on + strand, within Pf1N1B4_4477 at 4938.432 kb on - strand, within Pf1N1B4_4477 at 4938.533 kb on - strand, within Pf1N1B4_4477 at 4938.533 kb on - strand, within Pf1N1B4_4477 at 4938.619 kb on + strand, within Pf1N1B4_4477 at 4939.108 kb on + strand at 4939.181 kb on - strand at 4939.787 kb on - strand at 4939.933 kb on + strand, within Pf1N1B4_4478 at 4939.934 kb on - strand, within Pf1N1B4_4478 at 4939.934 kb on - strand, within Pf1N1B4_4478
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 4,936,918 + Pf1N1B4_4476 0.39 +0.2 4,936,919 - Pf1N1B4_4476 0.39 -1.6 4,936,978 + Pf1N1B4_4476 0.45 +0.2 4,936,978 + Pf1N1B4_4476 0.45 -0.4 4,936,979 - Pf1N1B4_4476 0.45 -1.1 4,936,979 - Pf1N1B4_4476 0.45 -0.8 4,936,979 - Pf1N1B4_4476 0.45 -0.4 4,937,387 - Pf1N1B4_4476 0.85 -1.4 4,937,500 + +1.8 4,937,501 - +0.6 4,937,554 - +0.4 4,937,616 + +0.4 4,937,616 + -0.7 4,937,616 + -0.1 4,937,616 + -0.1 4,937,616 + +0.6 4,937,616 + -0.4 4,937,617 - +2.2 4,937,617 - +1.9 4,937,617 - -2.0 4,937,617 - +0.6 4,937,617 - -1.1 4,937,617 - -0.1 4,937,617 - -0.3 4,937,780 - -1.3 4,938,254 - Pf1N1B4_4477 0.34 -2.0 4,938,431 + Pf1N1B4_4477 0.47 -1.8 4,938,431 + Pf1N1B4_4477 0.47 -2.6 4,938,432 - Pf1N1B4_4477 0.47 -0.1 4,938,533 - Pf1N1B4_4477 0.55 +2.2 4,938,533 - Pf1N1B4_4477 0.55 -1.4 4,938,619 + Pf1N1B4_4477 0.61 -0.8 4,939,108 + +0.8 4,939,181 - -0.6 4,939,787 - -0.4 4,939,933 + Pf1N1B4_4478 0.59 +0.3 4,939,934 - Pf1N1B4_4478 0.59 +0.6 4,939,934 - Pf1N1B4_4478 0.59 -0.6
Or see this region's nucleotide sequence