Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2505 and Pf1N1B4_2506 are separated by 215 nucleotides Pf1N1B4_2506 and Pf1N1B4_2507 are separated by 117 nucleotides Pf1N1B4_2507 and Pf1N1B4_2508 are separated by 574 nucleotides
Pf1N1B4_2505 - Lysine 2-monooxygenase (EC 1.13.12.2) (from data) , at 2,841,844 to 2,843,526
_2505
Pf1N1B4_2506 - Transcriptional regulator, AsnC family, at 2,843,742 to 2,844,203
_2506
Pf1N1B4_2507 - hypothetical protein, at 2,844,321 to 2,844,482
_2507
Pf1N1B4_2508 - hypothetical protein, at 2,845,057 to 2,845,365
_2508
Position (kb)
2844
2845 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2843.350 kb on + strand, within Pf1N1B4_2505 at 2843.413 kb on - strand at 2843.494 kb on - strand at 2843.570 kb on - strand at 2843.571 kb on + strand at 2843.572 kb on - strand at 2843.572 kb on - strand at 2843.572 kb on - strand at 2843.572 kb on - strand at 2843.594 kb on - strand at 2843.603 kb on - strand at 2843.616 kb on - strand at 2843.616 kb on - strand at 2843.616 kb on - strand at 2843.616 kb on - strand at 2843.672 kb on + strand at 2843.677 kb on - strand at 2843.677 kb on - strand at 2843.729 kb on + strand at 2843.878 kb on + strand, within Pf1N1B4_2506 at 2843.878 kb on + strand, within Pf1N1B4_2506 at 2843.878 kb on + strand, within Pf1N1B4_2506 at 2843.879 kb on - strand, within Pf1N1B4_2506 at 2843.983 kb on + strand, within Pf1N1B4_2506 at 2843.983 kb on + strand, within Pf1N1B4_2506 at 2843.983 kb on + strand, within Pf1N1B4_2506 at 2843.983 kb on + strand, within Pf1N1B4_2506 at 2843.983 kb on + strand, within Pf1N1B4_2506 at 2843.984 kb on - strand at 2843.984 kb on - strand, within Pf1N1B4_2506 at 2843.984 kb on - strand, within Pf1N1B4_2506 at 2843.984 kb on - strand, within Pf1N1B4_2506 at 2843.984 kb on - strand, within Pf1N1B4_2506 at 2844.244 kb on + strand at 2844.244 kb on + strand at 2844.245 kb on - strand at 2844.245 kb on - strand at 2844.299 kb on + strand at 2844.300 kb on - strand at 2844.322 kb on + strand at 2844.322 kb on + strand at 2844.322 kb on + strand at 2844.372 kb on + strand, within Pf1N1B4_2507 at 2844.373 kb on - strand, within Pf1N1B4_2507 at 2844.373 kb on - strand, within Pf1N1B4_2507 at 2844.408 kb on + strand, within Pf1N1B4_2507 at 2844.408 kb on + strand, within Pf1N1B4_2507 at 2844.409 kb on - strand, within Pf1N1B4_2507 at 2844.409 kb on - strand, within Pf1N1B4_2507 at 2844.415 kb on + strand at 2844.497 kb on + strand at 2844.497 kb on + strand at 2844.497 kb on + strand at 2844.556 kb on + strand at 2844.592 kb on + strand at 2844.592 kb on + strand at 2844.627 kb on - strand at 2844.688 kb on + strand at 2844.689 kb on - strand at 2844.689 kb on - strand at 2844.716 kb on + strand at 2844.716 kb on + strand at 2844.716 kb on + strand at 2844.716 kb on + strand at 2844.779 kb on + strand at 2844.780 kb on - strand at 2844.903 kb on - strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.906 kb on + strand at 2844.907 kb on - strand at 2844.907 kb on - strand at 2844.907 kb on - strand at 2844.985 kb on - strand at 2845.148 kb on + strand, within Pf1N1B4_2508 at 2845.148 kb on + strand, within Pf1N1B4_2508 at 2845.149 kb on - strand, within Pf1N1B4_2508 at 2845.149 kb on - strand, within Pf1N1B4_2508 at 2845.149 kb on - strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.301 kb on + strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.302 kb on - strand, within Pf1N1B4_2508 at 2845.303 kb on + strand, within Pf1N1B4_2508 at 2845.304 kb on - strand, within Pf1N1B4_2508 at 2845.304 kb on - strand, within Pf1N1B4_2508 at 2845.304 kb on - strand, within Pf1N1B4_2508 at 2845.304 kb on - strand, within Pf1N1B4_2508 at 2845.371 kb on + strand at 2845.371 kb on + strand at 2845.371 kb on + strand at 2845.372 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 2,843,350 + Pf1N1B4_2505 0.89 +0.8 2,843,413 - +1.4 2,843,494 - -1.5 2,843,570 - -0.9 2,843,571 + -0.9 2,843,572 - -1.6 2,843,572 - -0.6 2,843,572 - -1.0 2,843,572 - +1.2 2,843,594 - +0.4 2,843,603 - -1.5 2,843,616 - -1.2 2,843,616 - -0.0 2,843,616 - +0.5 2,843,616 - +0.2 2,843,672 + +0.2 2,843,677 - -0.8 2,843,677 - -0.3 2,843,729 + -0.1 2,843,878 + Pf1N1B4_2506 0.29 +0.2 2,843,878 + Pf1N1B4_2506 0.29 +0.1 2,843,878 + Pf1N1B4_2506 0.29 -0.8 2,843,879 - Pf1N1B4_2506 0.30 +0.2 2,843,983 + Pf1N1B4_2506 0.52 +0.1 2,843,983 + Pf1N1B4_2506 0.52 +0.6 2,843,983 + Pf1N1B4_2506 0.52 -0.9 2,843,983 + Pf1N1B4_2506 0.52 +0.6 2,843,983 + Pf1N1B4_2506 0.52 -1.5 2,843,984 - +1.8 2,843,984 - Pf1N1B4_2506 0.52 +1.2 2,843,984 - Pf1N1B4_2506 0.52 -1.9 2,843,984 - Pf1N1B4_2506 0.52 +1.1 2,843,984 - Pf1N1B4_2506 0.52 -1.0 2,844,244 + -1.3 2,844,244 + -0.6 2,844,245 - -0.6 2,844,245 - -0.6 2,844,299 + +0.2 2,844,300 - -1.3 2,844,322 + +0.4 2,844,322 + -1.3 2,844,322 + -1.3 2,844,372 + Pf1N1B4_2507 0.31 -2.4 2,844,373 - Pf1N1B4_2507 0.32 +1.1 2,844,373 - Pf1N1B4_2507 0.32 -0.8 2,844,408 + Pf1N1B4_2507 0.54 -0.2 2,844,408 + Pf1N1B4_2507 0.54 +0.2 2,844,409 - Pf1N1B4_2507 0.54 -1.3 2,844,409 - Pf1N1B4_2507 0.54 -1.2 2,844,415 + +1.2 2,844,497 + -1.2 2,844,497 + -1.9 2,844,497 + -0.5 2,844,556 + -0.6 2,844,592 + -0.8 2,844,592 + -0.1 2,844,627 - +0.8 2,844,688 + +0.1 2,844,689 - +0.2 2,844,689 - -1.0 2,844,716 + +0.2 2,844,716 + +0.4 2,844,716 + -1.6 2,844,716 + -1.1 2,844,779 + -0.2 2,844,780 - +0.2 2,844,903 - +0.1 2,844,906 + -1.2 2,844,906 + -1.6 2,844,906 + +1.4 2,844,906 + -0.5 2,844,906 + -1.2 2,844,906 + -1.3 2,844,906 + -0.8 2,844,906 + -0.8 2,844,906 + -0.5 2,844,907 - -0.6 2,844,907 - -0.7 2,844,907 - +2.3 2,844,985 - -0.8 2,845,148 + Pf1N1B4_2508 0.29 -0.2 2,845,148 + Pf1N1B4_2508 0.29 +1.2 2,845,149 - Pf1N1B4_2508 0.30 -1.1 2,845,149 - Pf1N1B4_2508 0.30 -1.6 2,845,149 - Pf1N1B4_2508 0.30 -0.2 2,845,301 + Pf1N1B4_2508 0.79 +1.4 2,845,301 + -0.5 2,845,301 + Pf1N1B4_2508 0.79 -2.4 2,845,301 + Pf1N1B4_2508 0.79 -0.7 2,845,301 + Pf1N1B4_2508 0.79 -1.1 2,845,301 + Pf1N1B4_2508 0.79 +0.0 2,845,301 + Pf1N1B4_2508 0.79 -0.3 2,845,301 + Pf1N1B4_2508 0.79 -0.2 2,845,301 + Pf1N1B4_2508 0.79 -0.1 2,845,301 + Pf1N1B4_2508 0.79 +0.0 2,845,301 + Pf1N1B4_2508 0.79 -0.4 2,845,301 + Pf1N1B4_2508 0.79 +0.6 2,845,301 + Pf1N1B4_2508 0.79 +0.0 2,845,301 + Pf1N1B4_2508 0.79 -0.0 2,845,302 - Pf1N1B4_2508 0.79 -0.8 2,845,302 - Pf1N1B4_2508 0.79 -1.8 2,845,302 - Pf1N1B4_2508 0.79 +1.1 2,845,302 - Pf1N1B4_2508 0.79 +1.8 2,845,302 - Pf1N1B4_2508 0.79 -1.5 2,845,302 - Pf1N1B4_2508 0.79 +0.2 2,845,302 - Pf1N1B4_2508 0.79 -1.7 2,845,302 - Pf1N1B4_2508 0.79 +0.1 2,845,302 - Pf1N1B4_2508 0.79 -0.2 2,845,302 - Pf1N1B4_2508 0.79 -1.1 2,845,302 - Pf1N1B4_2508 0.79 -1.0 2,845,302 - Pf1N1B4_2508 0.79 -0.5 2,845,302 - Pf1N1B4_2508 0.79 +1.2 2,845,302 - Pf1N1B4_2508 0.79 +1.8 2,845,302 - Pf1N1B4_2508 0.79 -2.1 2,845,302 - Pf1N1B4_2508 0.79 -0.8 2,845,303 + Pf1N1B4_2508 0.80 +1.2 2,845,304 - Pf1N1B4_2508 0.80 -0.7 2,845,304 - Pf1N1B4_2508 0.80 -1.2 2,845,304 - Pf1N1B4_2508 0.80 +0.8 2,845,304 - Pf1N1B4_2508 0.80 -1.0 2,845,371 + -0.5 2,845,371 + -0.9 2,845,371 + +0.6 2,845,372 - -1.0
Or see this region's nucleotide sequence