Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2418 and Pf1N1B4_2419 are separated by 322 nucleotides Pf1N1B4_2419 and Pf1N1B4_2420 are separated by 198 nucleotides
Pf1N1B4_2418 - HTH-type transcriptional regulator BetI, at 2,742,001 to 2,742,594
_2418
Pf1N1B4_2419 - High-affinity choline uptake protein BetT, at 2,742,917 to 2,744,923
_2419
Pf1N1B4_2420 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 2,745,122 to 2,746,300
_2420
Position (kb)
2742
2743
2744
2745 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 2741.918 kb on - strand at 2741.918 kb on - strand at 2741.918 kb on - strand at 2741.918 kb on - strand at 2741.947 kb on + strand at 2741.947 kb on + strand at 2741.947 kb on + strand at 2741.948 kb on - strand at 2741.948 kb on - strand at 2741.948 kb on - strand at 2742.002 kb on + strand at 2742.002 kb on + strand at 2742.003 kb on - strand at 2742.035 kb on + strand at 2742.035 kb on + strand at 2742.035 kb on + strand at 2742.035 kb on + strand at 2742.036 kb on - strand at 2742.036 kb on - strand at 2742.036 kb on - strand at 2742.036 kb on - strand at 2742.036 kb on - strand at 2742.163 kb on + strand, within Pf1N1B4_2418 at 2742.163 kb on + strand, within Pf1N1B4_2418 at 2742.164 kb on - strand, within Pf1N1B4_2418 at 2742.164 kb on - strand, within Pf1N1B4_2418 at 2742.194 kb on + strand, within Pf1N1B4_2418 at 2742.195 kb on - strand at 2742.322 kb on - strand, within Pf1N1B4_2418 at 2742.407 kb on + strand, within Pf1N1B4_2418 at 2742.408 kb on - strand, within Pf1N1B4_2418 at 2742.408 kb on - strand, within Pf1N1B4_2418 at 2742.408 kb on - strand, within Pf1N1B4_2418 at 2742.450 kb on - strand at 2742.450 kb on - strand, within Pf1N1B4_2418 at 2742.538 kb on + strand at 2742.538 kb on + strand at 2742.627 kb on + strand at 2742.627 kb on + strand at 2742.627 kb on + strand at 2742.627 kb on + strand at 2742.628 kb on - strand at 2742.628 kb on - strand at 2742.657 kb on - strand at 2742.657 kb on - strand at 2742.657 kb on - strand at 2742.732 kb on + strand at 2742.738 kb on + strand at 2742.738 kb on + strand at 2742.738 kb on + strand at 2742.738 kb on + strand at 2742.738 kb on + strand at 2742.738 kb on + strand at 2742.738 kb on + strand at 2742.738 kb on + strand at 2742.738 kb on + strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.739 kb on - strand at 2742.824 kb on + strand at 2742.824 kb on + strand at 2742.824 kb on + strand at 2742.824 kb on + strand at 2742.824 kb on + strand at 2742.824 kb on + strand at 2742.825 kb on - strand at 2742.825 kb on - strand at 2742.825 kb on - strand at 2742.825 kb on - strand at 2742.825 kb on - strand at 2742.825 kb on - strand at 2742.825 kb on - strand at 2742.825 kb on - strand at 2742.826 kb on + strand at 2742.827 kb on - strand at 2742.827 kb on - strand at 2742.827 kb on - strand at 2742.827 kb on - strand at 2742.827 kb on - strand at 2742.933 kb on + strand at 2742.934 kb on - strand at 2742.934 kb on - strand at 2742.936 kb on + strand at 2742.937 kb on - strand at 2742.980 kb on + strand at 2742.980 kb on + strand at 2742.980 kb on + strand at 2742.980 kb on + strand at 2742.981 kb on - strand at 2742.981 kb on - strand at 2742.981 kb on - strand at 2742.981 kb on - strand at 2742.981 kb on - strand at 2742.981 kb on - strand at 2742.981 kb on - strand at 2742.981 kb on - strand at 2742.981 kb on - strand at 2742.981 kb on - strand at 2742.981 kb on - strand at 2743.005 kb on + strand at 2743.006 kb on - strand at 2743.097 kb on + strand at 2743.097 kb on + strand at 2743.097 kb on + strand at 2743.097 kb on + strand at 2743.097 kb on + strand at 2743.098 kb on - strand at 2743.098 kb on - strand at 2743.098 kb on - strand at 2743.098 kb on - strand at 2743.098 kb on - strand at 2743.098 kb on - strand at 2743.098 kb on - strand at 2743.098 kb on - strand at 2743.098 kb on - strand at 2743.098 kb on - strand at 2743.098 kb on - strand at 2743.118 kb on + strand, within Pf1N1B4_2419 at 2743.119 kb on - strand, within Pf1N1B4_2419 at 2743.202 kb on + strand, within Pf1N1B4_2419 at 2743.203 kb on - strand, within Pf1N1B4_2419 at 2743.203 kb on - strand, within Pf1N1B4_2419 at 2743.203 kb on - strand, within Pf1N1B4_2419 at 2743.256 kb on + strand, within Pf1N1B4_2419 at 2743.286 kb on + strand, within Pf1N1B4_2419 at 2743.286 kb on + strand, within Pf1N1B4_2419 at 2743.286 kb on + strand, within Pf1N1B4_2419 at 2743.385 kb on + strand, within Pf1N1B4_2419 at 2743.385 kb on + strand, within Pf1N1B4_2419 at 2743.386 kb on - strand, within Pf1N1B4_2419 at 2743.386 kb on - strand, within Pf1N1B4_2419 at 2743.386 kb on - strand, within Pf1N1B4_2419 at 2743.386 kb on - strand, within Pf1N1B4_2419 at 2743.386 kb on - strand, within Pf1N1B4_2419 at 2743.386 kb on - strand, within Pf1N1B4_2419 at 2743.412 kb on + strand, within Pf1N1B4_2419 at 2743.413 kb on - strand, within Pf1N1B4_2419 at 2743.430 kb on - strand, within Pf1N1B4_2419 at 2743.847 kb on + strand, within Pf1N1B4_2419 at 2743.847 kb on + strand, within Pf1N1B4_2419 at 2743.847 kb on + strand, within Pf1N1B4_2419 at 2743.847 kb on + strand, within Pf1N1B4_2419 at 2743.848 kb on - strand, within Pf1N1B4_2419 at 2743.848 kb on - strand, within Pf1N1B4_2419 at 2743.848 kb on - strand, within Pf1N1B4_2419 at 2743.853 kb on + strand, within Pf1N1B4_2419 at 2743.853 kb on + strand, within Pf1N1B4_2419 at 2743.854 kb on - strand at 2743.854 kb on - strand, within Pf1N1B4_2419 at 2743.907 kb on + strand, within Pf1N1B4_2419 at 2743.907 kb on + strand, within Pf1N1B4_2419 at 2743.960 kb on + strand at 2743.961 kb on - strand, within Pf1N1B4_2419 at 2744.132 kb on + strand, within Pf1N1B4_2419 at 2744.132 kb on + strand, within Pf1N1B4_2419 at 2744.133 kb on - strand, within Pf1N1B4_2419 at 2744.150 kb on + strand, within Pf1N1B4_2419 at 2744.150 kb on + strand, within Pf1N1B4_2419 at 2744.706 kb on - strand at 2744.706 kb on - strand, within Pf1N1B4_2419 at 2744.706 kb on - strand, within Pf1N1B4_2419 at 2744.804 kb on + strand at 2744.804 kb on + strand at 2744.804 kb on + strand at 2744.805 kb on - strand at 2744.805 kb on - strand at 2744.828 kb on + strand at 2744.828 kb on + strand at 2744.915 kb on + strand at 2744.916 kb on - strand at 2744.917 kb on + strand at 2744.917 kb on + strand at 2744.917 kb on + strand at 2744.917 kb on + strand at 2744.917 kb on + strand at 2744.918 kb on - strand at 2744.918 kb on - strand at 2745.154 kb on - strand at 2745.154 kb on - strand at 2745.473 kb on + strand, within Pf1N1B4_2420 at 2745.473 kb on + strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.474 kb on - strand, within Pf1N1B4_2420 at 2745.513 kb on + strand, within Pf1N1B4_2420 at 2745.514 kb on - strand, within Pf1N1B4_2420 at 2745.514 kb on - strand, within Pf1N1B4_2420 at 2745.555 kb on + strand, within Pf1N1B4_2420
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 2,741,918 - +1.4 2,741,918 - -0.3 2,741,918 - +0.9 2,741,918 - +1.2 2,741,947 + -1.6 2,741,947 + -1.3 2,741,947 + -0.7 2,741,948 - -0.5 2,741,948 - -0.4 2,741,948 - -1.5 2,742,002 + -2.9 2,742,002 + -0.1 2,742,003 - -0.5 2,742,035 + -2.9 2,742,035 + -1.7 2,742,035 + -4.1 2,742,035 + -2.2 2,742,036 - +0.1 2,742,036 - +0.3 2,742,036 - -1.9 2,742,036 - -2.1 2,742,036 - -0.9 2,742,163 + Pf1N1B4_2418 0.27 -0.9 2,742,163 + Pf1N1B4_2418 0.27 +0.3 2,742,164 - Pf1N1B4_2418 0.27 -1.3 2,742,164 - Pf1N1B4_2418 0.27 -2.8 2,742,194 + Pf1N1B4_2418 0.32 -0.5 2,742,195 - +1.9 2,742,322 - Pf1N1B4_2418 0.54 -1.1 2,742,407 + Pf1N1B4_2418 0.68 -1.9 2,742,408 - Pf1N1B4_2418 0.69 -0.8 2,742,408 - Pf1N1B4_2418 0.69 -0.7 2,742,408 - Pf1N1B4_2418 0.69 -2.0 2,742,450 - +0.9 2,742,450 - Pf1N1B4_2418 0.76 -4.7 2,742,538 + -0.5 2,742,538 + -0.9 2,742,627 + -3.5 2,742,627 + -2.8 2,742,627 + +0.9 2,742,627 + -1.5 2,742,628 - +5.8 2,742,628 - +5.9 2,742,657 - +4.6 2,742,657 - +6.0 2,742,657 - +5.9 2,742,732 + -1.7 2,742,738 + -1.6 2,742,738 + -2.8 2,742,738 + -1.1 2,742,738 + -0.7 2,742,738 + -1.1 2,742,738 + -0.9 2,742,738 + -2.7 2,742,738 + -1.7 2,742,738 + -2.4 2,742,739 - +4.9 2,742,739 - +5.8 2,742,739 - +5.7 2,742,739 - +6.8 2,742,739 - +6.2 2,742,739 - +1.9 2,742,739 - +6.5 2,742,739 - +5.3 2,742,739 - +7.0 2,742,739 - +5.7 2,742,739 - +6.1 2,742,739 - +5.1 2,742,739 - +5.9 2,742,739 - +6.1 2,742,739 - +5.8 2,742,739 - +3.0 2,742,739 - +6.3 2,742,824 + -0.9 2,742,824 + +0.3 2,742,824 + -0.9 2,742,824 + -2.3 2,742,824 + +0.3 2,742,824 + -0.7 2,742,825 - +5.1 2,742,825 - +6.5 2,742,825 - +6.6 2,742,825 - +5.9 2,742,825 - +6.1 2,742,825 - +6.9 2,742,825 - +6.0 2,742,825 - +6.2 2,742,826 + -1.8 2,742,827 - +6.3 2,742,827 - +5.9 2,742,827 - +5.6 2,742,827 - +4.8 2,742,827 - +5.5 2,742,933 + +4.4 2,742,934 - +5.3 2,742,934 - +5.1 2,742,936 + +3.6 2,742,937 - +5.8 2,742,980 + +4.4 2,742,980 + +5.4 2,742,980 + +4.4 2,742,980 + +4.7 2,742,981 - +5.9 2,742,981 - +6.2 2,742,981 - +5.5 2,742,981 - +6.5 2,742,981 - +6.0 2,742,981 - +3.9 2,742,981 - +6.2 2,742,981 - +6.5 2,742,981 - +6.3 2,742,981 - +6.5 2,742,981 - +6.8 2,743,005 + +5.9 2,743,006 - +6.7 2,743,097 + +5.2 2,743,097 + +5.0 2,743,097 + +6.2 2,743,097 + +5.6 2,743,097 + +5.2 2,743,098 - +6.1 2,743,098 - +5.0 2,743,098 - +6.7 2,743,098 - +4.8 2,743,098 - +6.8 2,743,098 - +6.8 2,743,098 - +5.1 2,743,098 - +6.0 2,743,098 - +7.7 2,743,098 - +3.4 2,743,098 - +6.3 2,743,118 + Pf1N1B4_2419 0.10 +4.4 2,743,119 - Pf1N1B4_2419 0.10 +6.6 2,743,202 + Pf1N1B4_2419 0.14 +6.5 2,743,203 - Pf1N1B4_2419 0.14 +5.0 2,743,203 - Pf1N1B4_2419 0.14 +6.1 2,743,203 - Pf1N1B4_2419 0.14 +6.3 2,743,256 + Pf1N1B4_2419 0.17 +5.4 2,743,286 + Pf1N1B4_2419 0.18 +4.9 2,743,286 + Pf1N1B4_2419 0.18 +5.1 2,743,286 + Pf1N1B4_2419 0.18 +3.7 2,743,385 + Pf1N1B4_2419 0.23 +5.2 2,743,385 + Pf1N1B4_2419 0.23 +6.3 2,743,386 - Pf1N1B4_2419 0.23 +5.4 2,743,386 - Pf1N1B4_2419 0.23 +4.8 2,743,386 - Pf1N1B4_2419 0.23 +5.3 2,743,386 - Pf1N1B4_2419 0.23 +7.1 2,743,386 - Pf1N1B4_2419 0.23 +6.0 2,743,386 - Pf1N1B4_2419 0.23 +6.9 2,743,412 + Pf1N1B4_2419 0.25 +5.2 2,743,413 - Pf1N1B4_2419 0.25 +5.9 2,743,430 - Pf1N1B4_2419 0.26 +5.8 2,743,847 + Pf1N1B4_2419 0.46 +5.5 2,743,847 + Pf1N1B4_2419 0.46 +5.5 2,743,847 + Pf1N1B4_2419 0.46 +4.0 2,743,847 + Pf1N1B4_2419 0.46 +5.6 2,743,848 - Pf1N1B4_2419 0.46 +5.8 2,743,848 - Pf1N1B4_2419 0.46 +5.0 2,743,848 - Pf1N1B4_2419 0.46 +4.3 2,743,853 + Pf1N1B4_2419 0.47 +5.0 2,743,853 + Pf1N1B4_2419 0.47 +3.0 2,743,854 - +6.1 2,743,854 - Pf1N1B4_2419 0.47 +6.9 2,743,907 + Pf1N1B4_2419 0.49 +4.5 2,743,907 + Pf1N1B4_2419 0.49 +4.5 2,743,960 + +4.3 2,743,961 - Pf1N1B4_2419 0.52 +5.1 2,744,132 + Pf1N1B4_2419 0.61 +5.0 2,744,132 + Pf1N1B4_2419 0.61 +4.0 2,744,133 - Pf1N1B4_2419 0.61 +5.7 2,744,150 + Pf1N1B4_2419 0.61 +4.0 2,744,150 + Pf1N1B4_2419 0.61 +5.2 2,744,706 - +5.3 2,744,706 - Pf1N1B4_2419 0.89 +4.6 2,744,706 - Pf1N1B4_2419 0.89 +4.3 2,744,804 + +2.7 2,744,804 + +4.3 2,744,804 + +4.3 2,744,805 - -0.6 2,744,805 - +5.4 2,744,828 + +5.0 2,744,828 + +4.9 2,744,915 + +2.4 2,744,916 - +4.3 2,744,917 + +3.5 2,744,917 + +3.0 2,744,917 + +3.9 2,744,917 + +3.3 2,744,917 + +4.5 2,744,918 - +5.7 2,744,918 - +5.8 2,745,154 - +0.9 2,745,154 - +0.4 2,745,473 + Pf1N1B4_2420 0.30 +0.3 2,745,473 + Pf1N1B4_2420 0.30 +1.2 2,745,474 - Pf1N1B4_2420 0.30 -0.8 2,745,474 - Pf1N1B4_2420 0.30 -0.5 2,745,474 - Pf1N1B4_2420 0.30 +0.4 2,745,474 - Pf1N1B4_2420 0.30 +0.5 2,745,474 - Pf1N1B4_2420 0.30 +0.5 2,745,474 - Pf1N1B4_2420 0.30 -0.1 2,745,474 - Pf1N1B4_2420 0.30 -0.1 2,745,474 - Pf1N1B4_2420 0.30 -0.6 2,745,513 + Pf1N1B4_2420 0.33 +0.4 2,745,514 - Pf1N1B4_2420 0.33 +0.3 2,745,514 - Pf1N1B4_2420 0.33 +1.1 2,745,555 + Pf1N1B4_2420 0.37 -0.1
Or see this region's nucleotide sequence