Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1748 and Pf1N1B4_1749 are separated by 232 nucleotides Pf1N1B4_1749 and Pf1N1B4_1750 overlap by 4 nucleotides Pf1N1B4_1750 and Pf1N1B4_1751 are separated by 3 nucleotides Pf1N1B4_1751 and Pf1N1B4_1752 are separated by 134 nucleotides
Pf1N1B4_1748 - NADH:ubiquinone oxidoreductase subunit 2 (chain N), at 2,000,122 to 2,000,757
_1748
Pf1N1B4_1749 - Prevent host death protein, Phd antitoxin, at 2,000,990 to 2,001,268
_1749
Pf1N1B4_1750 - Death on curing protein, Doc toxin, at 2,001,265 to 2,001,546
_1750
Pf1N1B4_1751 - FIG00960943: hypothetical protein, at 2,001,550 to 2,001,795
_1751
Pf1N1B4_1752 - FIG00965470: hypothetical protein, at 2,001,930 to 2,002,391
_1752
Position (kb)
2001
2002 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2000.457 kb on - strand, within Pf1N1B4_1748 at 2000.472 kb on - strand, within Pf1N1B4_1748 at 2000.578 kb on + strand, within Pf1N1B4_1748 at 2000.579 kb on - strand, within Pf1N1B4_1748 at 2000.645 kb on - strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.758 kb on + strand at 2000.758 kb on + strand at 2000.759 kb on - strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.828 kb on + strand at 2000.828 kb on + strand at 2000.828 kb on + strand at 2000.828 kb on + strand at 2000.829 kb on - strand at 2000.829 kb on - strand at 2000.920 kb on + strand at 2000.920 kb on + strand at 2000.920 kb on + strand at 2000.921 kb on - strand at 2000.921 kb on - strand at 2000.921 kb on - strand at 2000.921 kb on - strand at 2000.942 kb on + strand at 2000.942 kb on + strand at 2000.942 kb on + strand at 2000.943 kb on - strand at 2000.943 kb on - strand at 2000.943 kb on - strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.953 kb on + strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.954 kb on - strand at 2000.955 kb on + strand at 2000.955 kb on + strand at 2000.955 kb on + strand at 2000.956 kb on - strand at 2000.956 kb on - strand at 2000.956 kb on - strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.957 kb on + strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.958 kb on - strand at 2000.968 kb on + strand at 2000.969 kb on - strand at 2000.969 kb on - strand at 2000.972 kb on + strand at 2001.161 kb on - strand, within Pf1N1B4_1749 at 2001.161 kb on - strand, within Pf1N1B4_1749 at 2001.161 kb on - strand, within Pf1N1B4_1749 at 2001.161 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.257 kb on - strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.258 kb on + strand at 2001.259 kb on - strand at 2001.259 kb on - strand at 2001.259 kb on - strand at 2001.272 kb on + strand at 2001.272 kb on + strand at 2001.273 kb on - strand at 2001.273 kb on - strand at 2001.273 kb on - strand at 2001.444 kb on - strand, within Pf1N1B4_1750 at 2001.516 kb on + strand, within Pf1N1B4_1750 at 2001.517 kb on - strand at 2001.517 kb on - strand, within Pf1N1B4_1750 at 2001.552 kb on - strand at 2001.720 kb on + strand, within Pf1N1B4_1751 at 2001.721 kb on - strand, within Pf1N1B4_1751 at 2001.817 kb on + strand at 2001.887 kb on + strand at 2001.888 kb on - strand at 2001.931 kb on + strand at 2001.931 kb on + strand at 2002.002 kb on + strand, within Pf1N1B4_1752 at 2002.003 kb on - strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.033 kb on + strand, within Pf1N1B4_1752 at 2002.034 kb on - strand, within Pf1N1B4_1752 at 2002.034 kb on - strand, within Pf1N1B4_1752 at 2002.037 kb on + strand, within Pf1N1B4_1752 at 2002.037 kb on + strand, within Pf1N1B4_1752 at 2002.037 kb on + strand, within Pf1N1B4_1752 at 2002.038 kb on - strand, within Pf1N1B4_1752 at 2002.038 kb on - strand, within Pf1N1B4_1752 at 2002.040 kb on - strand, within Pf1N1B4_1752 at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.231 kb on + strand at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.231 kb on + strand, within Pf1N1B4_1752 at 2002.232 kb on - strand, within Pf1N1B4_1752 at 2002.232 kb on - strand, within Pf1N1B4_1752 at 2002.232 kb on - strand, within Pf1N1B4_1752 at 2002.232 kb on - strand, within Pf1N1B4_1752 at 2002.345 kb on + strand at 2002.392 kb on - strand at 2002.456 kb on + strand at 2002.456 kb on + strand at 2002.457 kb on - strand at 2002.457 kb on - strand at 2002.464 kb on + strand at 2002.464 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 2,000,457 - Pf1N1B4_1748 0.53 +0.3 2,000,472 - Pf1N1B4_1748 0.55 -0.9 2,000,578 + Pf1N1B4_1748 0.72 +0.7 2,000,579 - Pf1N1B4_1748 0.72 -0.5 2,000,645 - Pf1N1B4_1748 0.82 +1.0 2,000,690 + Pf1N1B4_1748 0.89 +0.0 2,000,690 + Pf1N1B4_1748 0.89 -0.5 2,000,690 + Pf1N1B4_1748 0.89 -0.5 2,000,691 - Pf1N1B4_1748 0.89 +0.8 2,000,691 - Pf1N1B4_1748 0.89 -0.5 2,000,691 - Pf1N1B4_1748 0.89 +0.0 2,000,691 - Pf1N1B4_1748 0.89 +1.0 2,000,691 - Pf1N1B4_1748 0.89 -1.4 2,000,691 - Pf1N1B4_1748 0.89 -1.9 2,000,758 + -0.9 2,000,758 + +0.7 2,000,759 - +0.5 2,000,761 + -0.1 2,000,761 + -1.7 2,000,761 + -0.5 2,000,761 + +1.0 2,000,762 - +1.0 2,000,762 - +0.0 2,000,762 - +0.0 2,000,762 - -0.8 2,000,762 - -0.7 2,000,762 - -0.1 2,000,762 - -1.4 2,000,762 - -1.5 2,000,762 - +1.5 2,000,762 - -0.5 2,000,762 - -0.1 2,000,762 - +0.5 2,000,762 - -1.0 2,000,762 - +0.5 2,000,828 + +0.6 2,000,828 + +0.7 2,000,828 + -0.1 2,000,828 + -0.2 2,000,829 - -1.1 2,000,829 - +1.0 2,000,920 + -0.1 2,000,920 + -1.4 2,000,920 + +0.2 2,000,921 - +1.0 2,000,921 - +0.9 2,000,921 - -0.3 2,000,921 - +0.5 2,000,942 + -0.5 2,000,942 + -0.3 2,000,942 + +2.0 2,000,943 - -2.0 2,000,943 - +0.8 2,000,943 - +2.0 2,000,953 + -1.0 2,000,953 + -0.0 2,000,953 + -0.2 2,000,953 + +0.6 2,000,953 + -1.5 2,000,953 + -1.0 2,000,953 + +0.2 2,000,953 + +1.6 2,000,953 + +0.5 2,000,953 + -1.7 2,000,953 + -0.6 2,000,953 + +0.4 2,000,953 + -1.5 2,000,953 + -0.3 2,000,953 + -0.3 2,000,953 + -0.3 2,000,953 + +0.0 2,000,953 + -1.0 2,000,954 - +0.8 2,000,954 - +0.7 2,000,954 - -0.1 2,000,954 - +0.2 2,000,954 - +1.0 2,000,954 - +0.3 2,000,954 - +0.6 2,000,954 - +0.7 2,000,954 - +1.3 2,000,954 - +0.6 2,000,954 - +0.2 2,000,954 - -1.0 2,000,954 - -1.7 2,000,954 - +0.0 2,000,954 - +0.5 2,000,954 - +1.4 2,000,954 - -1.8 2,000,954 - +1.9 2,000,954 - -0.3 2,000,955 + -0.5 2,000,955 + +0.5 2,000,955 + +0.2 2,000,956 - +2.6 2,000,956 - +0.2 2,000,956 - +1.0 2,000,957 + -1.3 2,000,957 + +1.5 2,000,957 + +0.2 2,000,957 + +0.0 2,000,957 + +0.4 2,000,957 + -0.7 2,000,957 + -1.2 2,000,958 - -0.1 2,000,958 - +0.1 2,000,958 - -1.4 2,000,958 - -0.0 2,000,958 - -0.5 2,000,958 - -0.1 2,000,958 - +0.0 2,000,958 - -0.7 2,000,958 - +0.7 2,000,958 - +1.0 2,000,958 - +0.5 2,000,958 - -1.3 2,000,958 - +0.0 2,000,968 + -0.7 2,000,969 - -0.3 2,000,969 - -2.0 2,000,972 + -0.5 2,001,161 - Pf1N1B4_1749 0.61 -1.3 2,001,161 - Pf1N1B4_1749 0.61 +0.7 2,001,161 - Pf1N1B4_1749 0.61 -0.5 2,001,161 - +2.0 2,001,257 - +1.0 2,001,257 - -0.1 2,001,257 - +0.2 2,001,257 - -0.5 2,001,257 - +1.2 2,001,257 - -0.4 2,001,258 + -0.1 2,001,258 + +1.0 2,001,258 + +0.0 2,001,258 + -0.3 2,001,258 + +1.0 2,001,259 - -0.4 2,001,259 - -0.7 2,001,259 - -0.7 2,001,272 + -0.8 2,001,272 + +1.0 2,001,273 - -1.3 2,001,273 - -0.5 2,001,273 - +0.5 2,001,444 - Pf1N1B4_1750 0.63 +2.0 2,001,516 + Pf1N1B4_1750 0.89 -0.0 2,001,517 - -1.4 2,001,517 - Pf1N1B4_1750 0.89 +0.5 2,001,552 - +0.7 2,001,720 + Pf1N1B4_1751 0.69 -0.7 2,001,721 - Pf1N1B4_1751 0.70 +2.0 2,001,817 + +0.2 2,001,887 + +0.2 2,001,888 - -0.5 2,001,931 + -0.1 2,001,931 + +0.7 2,002,002 + Pf1N1B4_1752 0.16 +0.5 2,002,003 - Pf1N1B4_1752 0.16 -0.0 2,002,033 + Pf1N1B4_1752 0.22 +0.0 2,002,033 + Pf1N1B4_1752 0.22 -1.5 2,002,033 + Pf1N1B4_1752 0.22 -1.9 2,002,033 + Pf1N1B4_1752 0.22 -1.1 2,002,033 + Pf1N1B4_1752 0.22 -1.5 2,002,033 + Pf1N1B4_1752 0.22 -0.8 2,002,034 - Pf1N1B4_1752 0.23 +1.4 2,002,034 - Pf1N1B4_1752 0.23 +0.5 2,002,037 + Pf1N1B4_1752 0.23 +1.0 2,002,037 + Pf1N1B4_1752 0.23 -0.7 2,002,037 + Pf1N1B4_1752 0.23 -0.6 2,002,038 - Pf1N1B4_1752 0.23 -0.2 2,002,038 - Pf1N1B4_1752 0.23 +2.0 2,002,040 - Pf1N1B4_1752 0.24 +0.8 2,002,231 + Pf1N1B4_1752 0.65 -1.5 2,002,231 + Pf1N1B4_1752 0.65 -1.9 2,002,231 + Pf1N1B4_1752 0.65 +0.5 2,002,231 + Pf1N1B4_1752 0.65 -0.5 2,002,231 + +1.5 2,002,231 + Pf1N1B4_1752 0.65 -0.0 2,002,231 + Pf1N1B4_1752 0.65 -1.1 2,002,232 - Pf1N1B4_1752 0.65 -0.3 2,002,232 - Pf1N1B4_1752 0.65 +0.0 2,002,232 - Pf1N1B4_1752 0.65 +0.0 2,002,232 - Pf1N1B4_1752 0.65 +3.0 2,002,345 + -0.1 2,002,392 - -0.3 2,002,456 + -0.8 2,002,456 + +0.5 2,002,457 - +1.3 2,002,457 - +0.9 2,002,464 + -0.5 2,002,464 + -1.6
Or see this region's nucleotide sequence