Experiment: Paraquat dichloride 0.016 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1090 and Pf1N1B4_1091 are separated by 109 nucleotides Pf1N1B4_1091 and Pf1N1B4_1092 are separated by 48 nucleotides Pf1N1B4_1092 and Pf1N1B4_1093 are separated by 12 nucleotides
Pf1N1B4_1090 - TldE protein, part of TldE/TldD proteolytic complex, at 1,219,777 to 1,221,123
_1090
Pf1N1B4_1091 - FIG138315: Putative alpha helix protein, at 1,221,233 to 1,221,757
_1091
Pf1N1B4_1092 - TldD protein, part of TldE/TldD proteolytic complex, at 1,221,806 to 1,223,248
_1092
Pf1N1B4_1093 - FIG003879: Predicted amidohydrolase / Omega amidase (Nit2 homolog), at 1,223,261 to 1,224,115
_1093
Position (kb)
1221
1222
1223
1224 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1220.914 kb on + strand, within Pf1N1B4_1090 at 1220.915 kb on - strand, within Pf1N1B4_1090 at 1220.918 kb on + strand, within Pf1N1B4_1090 at 1220.918 kb on + strand, within Pf1N1B4_1090 at 1220.918 kb on + strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1220.919 kb on - strand, within Pf1N1B4_1090 at 1221.331 kb on + strand, within Pf1N1B4_1091 at 1221.331 kb on + strand at 1221.331 kb on + strand at 1221.331 kb on + strand, within Pf1N1B4_1091 at 1221.331 kb on + strand, within Pf1N1B4_1091 at 1221.380 kb on - strand, within Pf1N1B4_1091 at 1221.380 kb on - strand, within Pf1N1B4_1091 at 1221.421 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.442 kb on + strand, within Pf1N1B4_1091 at 1221.443 kb on - strand, within Pf1N1B4_1091 at 1221.443 kb on - strand, within Pf1N1B4_1091 at 1221.443 kb on - strand, within Pf1N1B4_1091 at 1221.443 kb on - strand, within Pf1N1B4_1091 at 1221.527 kb on + strand, within Pf1N1B4_1091 at 1221.527 kb on + strand, within Pf1N1B4_1091 at 1221.528 kb on - strand, within Pf1N1B4_1091 at 1221.716 kb on + strand at 1221.716 kb on + strand at 1221.717 kb on - strand at 1221.717 kb on - strand at 1221.717 kb on - strand at 1221.717 kb on - strand at 1221.717 kb on - strand at 1221.717 kb on - strand at 1221.808 kb on - strand at 1221.829 kb on + strand at 1221.830 kb on - strand at 1222.023 kb on + strand, within Pf1N1B4_1092 at 1222.095 kb on + strand, within Pf1N1B4_1092 at 1222.095 kb on + strand, within Pf1N1B4_1092 at 1222.095 kb on + strand, within Pf1N1B4_1092 at 1222.095 kb on + strand, within Pf1N1B4_1092 at 1222.095 kb on + strand, within Pf1N1B4_1092 at 1222.096 kb on - strand, within Pf1N1B4_1092 at 1222.096 kb on - strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.155 kb on + strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.156 kb on - strand, within Pf1N1B4_1092 at 1222.192 kb on - strand, within Pf1N1B4_1092 at 1222.258 kb on + strand, within Pf1N1B4_1092 at 1222.258 kb on + strand, within Pf1N1B4_1092 at 1222.258 kb on + strand, within Pf1N1B4_1092 at 1222.258 kb on + strand, within Pf1N1B4_1092 at 1222.258 kb on + strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand, within Pf1N1B4_1092 at 1222.259 kb on - strand at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.260 kb on + strand, within Pf1N1B4_1092 at 1222.261 kb on - strand, within Pf1N1B4_1092 at 1222.578 kb on + strand, within Pf1N1B4_1092 at 1222.609 kb on - strand, within Pf1N1B4_1092 at 1223.127 kb on + strand at 1223.248 kb on + strand at 1223.249 kb on - strand at 1223.249 kb on - strand at 1223.249 kb on - strand at 1223.255 kb on + strand at 1223.256 kb on - strand at 1223.262 kb on + strand at 1223.499 kb on + strand, within Pf1N1B4_1093 at 1223.499 kb on + strand, within Pf1N1B4_1093 at 1223.499 kb on + strand, within Pf1N1B4_1093 at 1223.499 kb on + strand, within Pf1N1B4_1093 at 1223.499 kb on + strand, within Pf1N1B4_1093 at 1223.499 kb on + strand, within Pf1N1B4_1093 at 1223.500 kb on - strand, within Pf1N1B4_1093 at 1223.703 kb on + strand, within Pf1N1B4_1093 at 1223.704 kb on - strand, within Pf1N1B4_1093 at 1223.709 kb on + strand, within Pf1N1B4_1093 at 1223.710 kb on - strand, within Pf1N1B4_1093 at 1223.753 kb on + strand, within Pf1N1B4_1093 at 1223.753 kb on + strand, within Pf1N1B4_1093 at 1223.879 kb on + strand, within Pf1N1B4_1093 at 1223.885 kb on + strand at 1224.151 kb on - strand at 1224.241 kb on + strand at 1224.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.016 mg/mL remove 1,220,914 + Pf1N1B4_1090 0.84 -0.1 1,220,915 - Pf1N1B4_1090 0.84 -1.7 1,220,918 + Pf1N1B4_1090 0.85 -1.7 1,220,918 + Pf1N1B4_1090 0.85 -1.6 1,220,918 + Pf1N1B4_1090 0.85 -1.3 1,220,919 - Pf1N1B4_1090 0.85 -1.1 1,220,919 - Pf1N1B4_1090 0.85 -0.3 1,220,919 - -0.6 1,220,919 - Pf1N1B4_1090 0.85 -0.6 1,220,919 - Pf1N1B4_1090 0.85 -1.1 1,220,919 - Pf1N1B4_1090 0.85 -0.6 1,220,919 - Pf1N1B4_1090 0.85 -0.3 1,220,919 - Pf1N1B4_1090 0.85 -0.3 1,220,919 - Pf1N1B4_1090 0.85 +0.2 1,220,919 - Pf1N1B4_1090 0.85 -0.6 1,221,331 + Pf1N1B4_1091 0.19 -0.8 1,221,331 + +0.2 1,221,331 + +0.8 1,221,331 + Pf1N1B4_1091 0.19 -1.2 1,221,331 + Pf1N1B4_1091 0.19 -0.6 1,221,380 - Pf1N1B4_1091 0.28 -0.9 1,221,380 - Pf1N1B4_1091 0.28 +0.4 1,221,421 + Pf1N1B4_1091 0.36 -0.1 1,221,442 + Pf1N1B4_1091 0.40 -1.2 1,221,442 + Pf1N1B4_1091 0.40 -0.2 1,221,442 + Pf1N1B4_1091 0.40 +0.8 1,221,442 + Pf1N1B4_1091 0.40 -0.4 1,221,442 + Pf1N1B4_1091 0.40 -1.1 1,221,442 + +1.3 1,221,442 + Pf1N1B4_1091 0.40 -0.1 1,221,442 + Pf1N1B4_1091 0.40 -1.4 1,221,442 + Pf1N1B4_1091 0.40 +1.2 1,221,442 + Pf1N1B4_1091 0.40 +0.0 1,221,442 + Pf1N1B4_1091 0.40 -0.8 1,221,442 + Pf1N1B4_1091 0.40 +1.3 1,221,442 + Pf1N1B4_1091 0.40 +1.8 1,221,443 - Pf1N1B4_1091 0.40 +0.9 1,221,443 - Pf1N1B4_1091 0.40 +0.8 1,221,443 - Pf1N1B4_1091 0.40 +1.4 1,221,443 - Pf1N1B4_1091 0.40 -1.2 1,221,527 + Pf1N1B4_1091 0.56 -0.6 1,221,527 + Pf1N1B4_1091 0.56 -1.1 1,221,528 - Pf1N1B4_1091 0.56 -0.0 1,221,716 + -0.2 1,221,716 + -0.2 1,221,717 - -0.2 1,221,717 - -0.1 1,221,717 - -1.9 1,221,717 - +0.9 1,221,717 - +0.6 1,221,717 - -0.6 1,221,808 - -0.6 1,221,829 + -0.2 1,221,830 - +1.2 1,222,023 + Pf1N1B4_1092 0.15 -1.1 1,222,095 + Pf1N1B4_1092 0.20 -0.2 1,222,095 + Pf1N1B4_1092 0.20 -1.1 1,222,095 + Pf1N1B4_1092 0.20 -0.8 1,222,095 + Pf1N1B4_1092 0.20 -0.1 1,222,095 + Pf1N1B4_1092 0.20 -1.0 1,222,096 - Pf1N1B4_1092 0.20 -2.0 1,222,096 - Pf1N1B4_1092 0.20 +1.8 1,222,155 + Pf1N1B4_1092 0.24 -1.6 1,222,155 + Pf1N1B4_1092 0.24 -0.7 1,222,155 + Pf1N1B4_1092 0.24 -0.4 1,222,155 + Pf1N1B4_1092 0.24 -1.2 1,222,155 + Pf1N1B4_1092 0.24 -0.4 1,222,155 + Pf1N1B4_1092 0.24 -0.8 1,222,155 + Pf1N1B4_1092 0.24 -2.0 1,222,155 + Pf1N1B4_1092 0.24 +0.2 1,222,156 - Pf1N1B4_1092 0.24 -0.6 1,222,156 - Pf1N1B4_1092 0.24 -0.2 1,222,156 - Pf1N1B4_1092 0.24 -0.6 1,222,156 - Pf1N1B4_1092 0.24 -0.1 1,222,156 - Pf1N1B4_1092 0.24 -1.2 1,222,156 - Pf1N1B4_1092 0.24 +0.8 1,222,156 - Pf1N1B4_1092 0.24 -1.2 1,222,156 - Pf1N1B4_1092 0.24 -1.2 1,222,156 - Pf1N1B4_1092 0.24 -0.1 1,222,156 - +0.2 1,222,156 - Pf1N1B4_1092 0.24 +0.2 1,222,156 - Pf1N1B4_1092 0.24 -0.8 1,222,192 - Pf1N1B4_1092 0.27 -1.1 1,222,258 + Pf1N1B4_1092 0.31 -0.1 1,222,258 + Pf1N1B4_1092 0.31 -1.2 1,222,258 + Pf1N1B4_1092 0.31 -1.2 1,222,258 + Pf1N1B4_1092 0.31 -1.2 1,222,258 + Pf1N1B4_1092 0.31 -1.4 1,222,259 - Pf1N1B4_1092 0.31 +3.1 1,222,259 - Pf1N1B4_1092 0.31 +0.3 1,222,259 - Pf1N1B4_1092 0.31 +0.8 1,222,259 - Pf1N1B4_1092 0.31 +1.2 1,222,259 - Pf1N1B4_1092 0.31 -2.1 1,222,259 - Pf1N1B4_1092 0.31 -1.1 1,222,259 - Pf1N1B4_1092 0.31 -0.8 1,222,259 - +0.8 1,222,260 + Pf1N1B4_1092 0.31 +1.0 1,222,260 + Pf1N1B4_1092 0.31 -1.2 1,222,260 + Pf1N1B4_1092 0.31 +0.2 1,222,260 + Pf1N1B4_1092 0.31 +0.8 1,222,260 + Pf1N1B4_1092 0.31 -0.7 1,222,260 + Pf1N1B4_1092 0.31 +0.2 1,222,261 - Pf1N1B4_1092 0.32 -0.8 1,222,578 + Pf1N1B4_1092 0.53 +1.8 1,222,609 - Pf1N1B4_1092 0.56 -0.2 1,223,127 + -0.2 1,223,248 + -0.8 1,223,249 - -2.1 1,223,249 - +0.4 1,223,249 - +1.8 1,223,255 + +0.4 1,223,256 - +0.8 1,223,262 + -1.1 1,223,499 + Pf1N1B4_1093 0.28 -0.2 1,223,499 + Pf1N1B4_1093 0.28 -1.4 1,223,499 + Pf1N1B4_1093 0.28 +1.2 1,223,499 + Pf1N1B4_1093 0.28 -0.1 1,223,499 + Pf1N1B4_1093 0.28 +0.4 1,223,499 + Pf1N1B4_1093 0.28 -0.8 1,223,500 - Pf1N1B4_1093 0.28 +0.4 1,223,703 + Pf1N1B4_1093 0.52 -0.2 1,223,704 - Pf1N1B4_1093 0.52 -0.8 1,223,709 + Pf1N1B4_1093 0.52 +0.5 1,223,710 - Pf1N1B4_1093 0.53 +1.2 1,223,753 + Pf1N1B4_1093 0.58 +0.8 1,223,753 + Pf1N1B4_1093 0.58 +1.8 1,223,879 + Pf1N1B4_1093 0.72 +1.8 1,223,885 + -0.6 1,224,151 - +0.4 1,224,241 + +1.8 1,224,242 - -1.1
Or see this region's nucleotide sequence