Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS05215

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS05210 and MMJJ_RS05215 are separated by 13 nucleotidesMMJJ_RS05215 and MMJJ_RS05220 are separated by 72 nucleotides MMJJ_RS05210: MMJJ_RS05210 - polyprenyl synthetase family protein, at 961,776 to 962,729 _RS05210 MMJJ_RS05215: MMJJ_RS05215 - RNase J family beta-CASP ribonuclease, at 962,743 to 964,089 _RS05215 MMJJ_RS05220: MMJJ_RS05220 - type 2 isopentenyl-diphosphate Delta-isomerase, at 964,162 to 965,229 _RS05220 Position (kb) 962 963 964 965Strain fitness (log2 ratio) -2 -1 0 1 2 3at 961.763 kb on - strandat 961.764 kb on + strandat 961.767 kb on - strandat 961.768 kb on + strandat 962.146 kb on - strand, within MMJJ_RS05210at 962.745 kb on + strandat 963.772 kb on + strand, within MMJJ_RS05215at 963.898 kb on + strand, within MMJJ_RS05215at 964.671 kb on - strand, within MMJJ_RS05220at 964.958 kb on + strand, within MMJJ_RS05220at 964.980 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
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961,763 - +2.9
961,764 + +0.1
961,767 - -0.1
961,768 + +0.1
962,146 - MMJJ_RS05210 0.39 -1.0
962,745 + -1.7
963,772 + MMJJ_RS05215 0.76 -1.3
963,898 + MMJJ_RS05215 0.86 -0.7
964,671 - MMJJ_RS05220 0.48 -0.7
964,958 + MMJJ_RS05220 0.75 -2.1
964,980 - -1.3

Or see this region's nucleotide sequence