Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02200 and MMJJ_RS02205 are separated by 33 nucleotides MMJJ_RS02205 and MMJJ_RS02210 are separated by 125 nucleotides MMJJ_RS02210 and MMJJ_RS02215 are separated by 36 nucleotides MMJJ_RS02215 and MMJJ_RS02220 are separated by 98 nucleotides
MMJJ_RS02200: MMJJ_RS02200 - chorismate pyruvate-lyase family protein, at 418,331 to 418,846
_RS02200
MMJJ_RS02205: MMJJ_RS02205 - hypothetical protein, at 418,880 to 419,482
_RS02205
MMJJ_RS02210: MMJJ_RS02210 - phosphoserine phosphatase SerB, at 419,608 to 420,249
_RS02210
MMJJ_RS02215: MMJJ_RS02215 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 420,286 to 421,026
_RS02215
MMJJ_RS02220: MMJJ_RS02220 - 3-isopropylmalate dehydrogenase, at 421,125 to 422,117
_RS02220
Position (kb) 419
420
421 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 418.620 kb on - strand, within MMJJ_RS02200 at 418.621 kb on + strand, within MMJJ_RS02200 at 418.621 kb on + strand, within MMJJ_RS02200 at 418.621 kb on + strand, within MMJJ_RS02200 at 418.622 kb on - strand, within MMJJ_RS02200 at 418.622 kb on - strand, within MMJJ_RS02200 at 418.622 kb on - strand, within MMJJ_RS02200 at 418.623 kb on + strand, within MMJJ_RS02200 at 418.641 kb on + strand, within MMJJ_RS02200 at 418.641 kb on + strand, within MMJJ_RS02200 at 418.653 kb on + strand, within MMJJ_RS02200 at 418.668 kb on + strand, within MMJJ_RS02200 at 418.679 kb on - strand, within MMJJ_RS02200 at 418.689 kb on - strand, within MMJJ_RS02200 at 418.793 kb on - strand, within MMJJ_RS02200 at 418.811 kb on - strand at 418.834 kb on + strand at 418.857 kb on + strand at 418.923 kb on + strand at 418.923 kb on + strand at 418.932 kb on - strand at 418.934 kb on - strand at 418.955 kb on - strand, within MMJJ_RS02205 at 418.956 kb on + strand, within MMJJ_RS02205 at 418.974 kb on - strand, within MMJJ_RS02205 at 419.009 kb on - strand, within MMJJ_RS02205 at 419.010 kb on + strand, within MMJJ_RS02205 at 419.117 kb on - strand, within MMJJ_RS02205 at 419.159 kb on + strand, within MMJJ_RS02205 at 419.182 kb on - strand, within MMJJ_RS02205 at 419.183 kb on + strand, within MMJJ_RS02205 at 419.183 kb on + strand, within MMJJ_RS02205 at 419.183 kb on + strand, within MMJJ_RS02205 at 419.194 kb on - strand, within MMJJ_RS02205 at 419.194 kb on - strand, within MMJJ_RS02205 at 419.206 kb on - strand, within MMJJ_RS02205 at 419.207 kb on + strand, within MMJJ_RS02205 at 419.213 kb on - strand, within MMJJ_RS02205 at 419.238 kb on - strand, within MMJJ_RS02205 at 419.245 kb on - strand, within MMJJ_RS02205 at 419.245 kb on - strand, within MMJJ_RS02205 at 419.245 kb on - strand, within MMJJ_RS02205 at 419.246 kb on + strand, within MMJJ_RS02205 at 419.246 kb on + strand, within MMJJ_RS02205 at 419.250 kb on + strand, within MMJJ_RS02205 at 419.298 kb on + strand, within MMJJ_RS02205 at 419.309 kb on + strand, within MMJJ_RS02205 at 419.333 kb on - strand, within MMJJ_RS02205 at 419.335 kb on - strand, within MMJJ_RS02205 at 419.336 kb on + strand, within MMJJ_RS02205 at 419.339 kb on - strand, within MMJJ_RS02205 at 419.339 kb on - strand, within MMJJ_RS02205 at 419.340 kb on + strand, within MMJJ_RS02205 at 419.340 kb on + strand, within MMJJ_RS02205 at 419.340 kb on + strand, within MMJJ_RS02205 at 419.341 kb on - strand, within MMJJ_RS02205 at 419.342 kb on + strand, within MMJJ_RS02205 at 419.352 kb on + strand, within MMJJ_RS02205 at 419.441 kb on + strand at 419.441 kb on + strand at 419.441 kb on + strand at 419.444 kb on - strand at 419.504 kb on - strand at 419.504 kb on - strand at 419.505 kb on + strand at 419.510 kb on + strand at 419.510 kb on + strand at 419.510 kb on + strand at 419.513 kb on + strand at 419.513 kb on + strand at 419.527 kb on - strand at 419.528 kb on + strand at 419.528 kb on + strand at 419.532 kb on - strand at 419.533 kb on + strand at 419.533 kb on + strand at 419.533 kb on + strand at 419.534 kb on - strand at 419.534 kb on - strand at 419.537 kb on + strand at 419.537 kb on + strand at 419.550 kb on - strand at 419.585 kb on - strand at 420.641 kb on - strand, within MMJJ_RS02215 at 421.053 kb on - strand at 421.065 kb on - strand at 421.073 kb on - strand at 421.074 kb on + strand at 421.082 kb on - strand at 421.086 kb on + strand at 421.115 kb on - strand at 421.117 kb on - strand at 421.118 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 418,620 - MMJJ_RS02200 0.56 -2.2 418,621 + MMJJ_RS02200 0.56 +0.8 418,621 + MMJJ_RS02200 0.56 -1.4 418,621 + MMJJ_RS02200 0.56 -2.7 418,622 - MMJJ_RS02200 0.56 -3.5 418,622 - MMJJ_RS02200 0.56 -2.7 418,622 - MMJJ_RS02200 0.56 -3.3 418,623 + MMJJ_RS02200 0.57 -3.7 418,641 + MMJJ_RS02200 0.60 -2.7 418,641 + MMJJ_RS02200 0.60 -1.8 418,653 + MMJJ_RS02200 0.62 -2.4 418,668 + MMJJ_RS02200 0.65 -3.2 418,679 - MMJJ_RS02200 0.67 -1.7 418,689 - MMJJ_RS02200 0.69 -2.7 418,793 - MMJJ_RS02200 0.90 -1.8 418,811 - -2.2 418,834 + -4.0 418,857 + -3.7 418,923 + -1.8 418,923 + -2.9 418,932 - -0.8 418,934 - -1.2 418,955 - MMJJ_RS02205 0.12 -0.6 418,956 + MMJJ_RS02205 0.13 -2.1 418,974 - MMJJ_RS02205 0.16 -0.6 419,009 - MMJJ_RS02205 0.21 -1.8 419,010 + MMJJ_RS02205 0.22 -3.3 419,117 - MMJJ_RS02205 0.39 -1.0 419,159 + MMJJ_RS02205 0.46 -2.4 419,182 - MMJJ_RS02205 0.50 -1.4 419,183 + MMJJ_RS02205 0.50 -1.8 419,183 + MMJJ_RS02205 0.50 -3.1 419,183 + MMJJ_RS02205 0.50 -2.0 419,194 - MMJJ_RS02205 0.52 -0.8 419,194 - MMJJ_RS02205 0.52 -1.4 419,206 - MMJJ_RS02205 0.54 -0.8 419,207 + MMJJ_RS02205 0.54 -3.5 419,213 - MMJJ_RS02205 0.55 -1.4 419,238 - MMJJ_RS02205 0.59 -1.7 419,245 - MMJJ_RS02205 0.61 -1.4 419,245 - MMJJ_RS02205 0.61 +0.2 419,245 - MMJJ_RS02205 0.61 -1.4 419,246 + MMJJ_RS02205 0.61 -2.3 419,246 + MMJJ_RS02205 0.61 -2.9 419,250 + MMJJ_RS02205 0.61 -2.5 419,298 + MMJJ_RS02205 0.69 +0.2 419,309 + MMJJ_RS02205 0.71 -3.1 419,333 - MMJJ_RS02205 0.75 -0.2 419,335 - MMJJ_RS02205 0.75 +0.4 419,336 + MMJJ_RS02205 0.76 -1.2 419,339 - MMJJ_RS02205 0.76 -0.9 419,339 - MMJJ_RS02205 0.76 -1.2 419,340 + MMJJ_RS02205 0.76 -3.6 419,340 + MMJJ_RS02205 0.76 -2.5 419,340 + MMJJ_RS02205 0.76 -4.5 419,341 - MMJJ_RS02205 0.76 -2.0 419,342 + MMJJ_RS02205 0.77 -2.4 419,352 + MMJJ_RS02205 0.78 -0.6 419,441 + +0.1 419,441 + -2.8 419,441 + -0.7 419,444 - +0.5 419,504 - -1.4 419,504 - -0.8 419,505 + +0.2 419,510 + -1.0 419,510 + -1.3 419,510 + -1.7 419,513 + -1.1 419,513 + -0.3 419,527 - -1.0 419,528 + -0.8 419,528 + -0.0 419,532 - -0.6 419,533 + -1.1 419,533 + -0.2 419,533 + -4.2 419,534 - +2.8 419,534 - +0.2 419,537 + +0.7 419,537 + +0.7 419,550 - +1.4 419,585 - +0.6 420,641 - MMJJ_RS02215 0.48 -2.2 421,053 - +0.2 421,065 - -0.8 421,073 - -3.0 421,074 + -1.4 421,082 - -2.7 421,086 + -3.2 421,115 - -3.0 421,117 - -1.4 421,118 + -2.7
Or see this region's nucleotide sequence