Experiment: Cisplatin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3210 and Pf6N2E2_3211 overlap by 17 nucleotides Pf6N2E2_3211 and Pf6N2E2_3212 are separated by 1 nucleotides
Pf6N2E2_3210 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 3,707,891 to 3,708,976
_3210
Pf6N2E2_3211 - Outer membrane receptor proteins, mostly Fe transport, at 3,708,960 to 3,711,311
_3211
Pf6N2E2_3212 - Signal transduction histidine kinase, at 3,711,313 to 3,713,712
_3212
Position (kb)
3708
3709
3710
3711
3712 Strain fitness (log2 ratio)
-2
-1
0
1 at 3707.990 kb on + strand at 3707.991 kb on - strand at 3708.255 kb on - strand, within Pf6N2E2_3210 at 3708.255 kb on - strand, within Pf6N2E2_3210 at 3708.380 kb on - strand, within Pf6N2E2_3210 at 3708.392 kb on + strand, within Pf6N2E2_3210 at 3708.392 kb on + strand, within Pf6N2E2_3210 at 3708.392 kb on + strand, within Pf6N2E2_3210 at 3708.393 kb on - strand, within Pf6N2E2_3210 at 3708.393 kb on - strand, within Pf6N2E2_3210 at 3708.393 kb on - strand, within Pf6N2E2_3210 at 3708.393 kb on - strand, within Pf6N2E2_3210 at 3708.442 kb on - strand, within Pf6N2E2_3210 at 3708.442 kb on - strand, within Pf6N2E2_3210 at 3708.602 kb on + strand, within Pf6N2E2_3210 at 3708.602 kb on + strand, within Pf6N2E2_3210 at 3708.603 kb on - strand, within Pf6N2E2_3210 at 3708.899 kb on + strand at 3708.899 kb on + strand at 3708.900 kb on - strand at 3708.900 kb on - strand at 3708.900 kb on - strand at 3708.952 kb on + strand at 3708.952 kb on + strand at 3708.953 kb on - strand at 3709.018 kb on + strand at 3709.019 kb on - strand at 3709.042 kb on + strand at 3709.042 kb on + strand at 3709.042 kb on + strand at 3709.057 kb on + strand at 3709.057 kb on + strand at 3709.058 kb on - strand at 3709.148 kb on - strand at 3709.159 kb on + strand at 3709.159 kb on + strand at 3709.160 kb on - strand at 3709.160 kb on - strand at 3709.210 kb on + strand, within Pf6N2E2_3211 at 3709.211 kb on - strand, within Pf6N2E2_3211 at 3709.297 kb on + strand, within Pf6N2E2_3211 at 3709.297 kb on + strand, within Pf6N2E2_3211 at 3709.297 kb on + strand, within Pf6N2E2_3211 at 3709.297 kb on + strand, within Pf6N2E2_3211 at 3709.297 kb on + strand, within Pf6N2E2_3211 at 3709.525 kb on + strand, within Pf6N2E2_3211 at 3709.525 kb on + strand, within Pf6N2E2_3211 at 3709.525 kb on + strand, within Pf6N2E2_3211 at 3709.525 kb on + strand, within Pf6N2E2_3211 at 3709.529 kb on + strand, within Pf6N2E2_3211 at 3709.529 kb on + strand, within Pf6N2E2_3211 at 3709.529 kb on + strand, within Pf6N2E2_3211 at 3709.530 kb on - strand, within Pf6N2E2_3211 at 3709.540 kb on + strand, within Pf6N2E2_3211 at 3709.540 kb on + strand, within Pf6N2E2_3211 at 3709.541 kb on - strand, within Pf6N2E2_3211 at 3709.561 kb on + strand, within Pf6N2E2_3211 at 3709.700 kb on - strand, within Pf6N2E2_3211 at 3709.720 kb on + strand, within Pf6N2E2_3211 at 3709.720 kb on + strand, within Pf6N2E2_3211 at 3709.721 kb on - strand, within Pf6N2E2_3211 at 3709.747 kb on + strand, within Pf6N2E2_3211 at 3710.023 kb on + strand, within Pf6N2E2_3211 at 3710.089 kb on + strand, within Pf6N2E2_3211 at 3710.189 kb on - strand, within Pf6N2E2_3211 at 3710.191 kb on + strand, within Pf6N2E2_3211 at 3710.192 kb on - strand, within Pf6N2E2_3211 at 3710.192 kb on - strand, within Pf6N2E2_3211 at 3710.206 kb on + strand, within Pf6N2E2_3211 at 3710.461 kb on + strand, within Pf6N2E2_3211 at 3710.462 kb on - strand, within Pf6N2E2_3211 at 3710.462 kb on - strand, within Pf6N2E2_3211 at 3710.569 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.971 kb on + strand, within Pf6N2E2_3211 at 3710.972 kb on - strand, within Pf6N2E2_3211 at 3710.972 kb on - strand, within Pf6N2E2_3211 at 3711.314 kb on + strand at 3711.314 kb on + strand at 3711.314 kb on + strand at 3711.636 kb on - strand, within Pf6N2E2_3212 at 3711.654 kb on - strand, within Pf6N2E2_3212 at 3711.702 kb on + strand, within Pf6N2E2_3212 at 3711.891 kb on - strand, within Pf6N2E2_3212 at 3711.891 kb on - strand, within Pf6N2E2_3212 at 3712.198 kb on + strand, within Pf6N2E2_3212 at 3712.198 kb on + strand, within Pf6N2E2_3212 at 3712.199 kb on - strand, within Pf6N2E2_3212 at 3712.218 kb on - strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.219 kb on + strand, within Pf6N2E2_3212 at 3712.220 kb on - strand, within Pf6N2E2_3212 at 3712.220 kb on - strand, within Pf6N2E2_3212 at 3712.220 kb on - strand, within Pf6N2E2_3212 at 3712.220 kb on - strand, within Pf6N2E2_3212 at 3712.220 kb on - strand, within Pf6N2E2_3212 at 3712.223 kb on + strand, within Pf6N2E2_3212 at 3712.224 kb on - strand, within Pf6N2E2_3212
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.008 mg/ml remove 3,707,990 + +0.3 3,707,991 - -0.1 3,708,255 - Pf6N2E2_3210 0.34 -0.7 3,708,255 - Pf6N2E2_3210 0.34 -0.5 3,708,380 - Pf6N2E2_3210 0.45 +1.2 3,708,392 + Pf6N2E2_3210 0.46 +0.1 3,708,392 + Pf6N2E2_3210 0.46 -0.6 3,708,392 + Pf6N2E2_3210 0.46 +0.2 3,708,393 - Pf6N2E2_3210 0.46 +0.4 3,708,393 - Pf6N2E2_3210 0.46 +0.5 3,708,393 - Pf6N2E2_3210 0.46 +0.3 3,708,393 - Pf6N2E2_3210 0.46 +0.9 3,708,442 - Pf6N2E2_3210 0.51 +0.0 3,708,442 - Pf6N2E2_3210 0.51 +1.2 3,708,602 + Pf6N2E2_3210 0.65 +0.5 3,708,602 + Pf6N2E2_3210 0.65 -1.6 3,708,603 - Pf6N2E2_3210 0.66 -0.8 3,708,899 + +0.7 3,708,899 + -1.1 3,708,900 - +1.0 3,708,900 - -0.9 3,708,900 - -0.7 3,708,952 + +1.4 3,708,952 + -0.4 3,708,953 - +1.0 3,709,018 + +0.1 3,709,019 - +0.5 3,709,042 + +0.4 3,709,042 + -0.2 3,709,042 + +0.7 3,709,057 + -0.6 3,709,057 + -0.2 3,709,058 - +0.8 3,709,148 - -1.6 3,709,159 + +0.5 3,709,159 + -0.3 3,709,160 - -0.3 3,709,160 - +1.0 3,709,210 + Pf6N2E2_3211 0.11 -1.4 3,709,211 - Pf6N2E2_3211 0.11 +0.2 3,709,297 + Pf6N2E2_3211 0.14 +0.5 3,709,297 + Pf6N2E2_3211 0.14 -0.1 3,709,297 + Pf6N2E2_3211 0.14 +0.1 3,709,297 + Pf6N2E2_3211 0.14 -1.0 3,709,297 + Pf6N2E2_3211 0.14 -0.2 3,709,525 + Pf6N2E2_3211 0.24 -1.3 3,709,525 + Pf6N2E2_3211 0.24 -2.1 3,709,525 + Pf6N2E2_3211 0.24 -0.2 3,709,525 + Pf6N2E2_3211 0.24 +0.0 3,709,529 + Pf6N2E2_3211 0.24 -0.1 3,709,529 + Pf6N2E2_3211 0.24 +0.1 3,709,529 + Pf6N2E2_3211 0.24 -0.2 3,709,530 - Pf6N2E2_3211 0.24 +0.5 3,709,540 + Pf6N2E2_3211 0.25 -0.2 3,709,540 + Pf6N2E2_3211 0.25 -0.7 3,709,541 - Pf6N2E2_3211 0.25 -0.0 3,709,561 + Pf6N2E2_3211 0.26 -1.4 3,709,700 - Pf6N2E2_3211 0.31 +0.2 3,709,720 + Pf6N2E2_3211 0.32 +0.3 3,709,720 + Pf6N2E2_3211 0.32 -2.1 3,709,721 - Pf6N2E2_3211 0.32 +0.4 3,709,747 + Pf6N2E2_3211 0.33 +0.1 3,710,023 + Pf6N2E2_3211 0.45 -1.5 3,710,089 + Pf6N2E2_3211 0.48 -1.5 3,710,189 - Pf6N2E2_3211 0.52 +0.5 3,710,191 + Pf6N2E2_3211 0.52 -0.6 3,710,192 - Pf6N2E2_3211 0.52 -1.1 3,710,192 - Pf6N2E2_3211 0.52 +0.2 3,710,206 + Pf6N2E2_3211 0.53 -0.7 3,710,461 + Pf6N2E2_3211 0.64 +0.0 3,710,462 - Pf6N2E2_3211 0.64 +0.3 3,710,462 - Pf6N2E2_3211 0.64 -0.2 3,710,569 + Pf6N2E2_3211 0.68 +0.2 3,710,971 + Pf6N2E2_3211 0.86 -0.6 3,710,971 + Pf6N2E2_3211 0.86 -0.3 3,710,971 + Pf6N2E2_3211 0.86 +0.7 3,710,971 + Pf6N2E2_3211 0.86 -0.7 3,710,971 + Pf6N2E2_3211 0.86 -0.8 3,710,971 + Pf6N2E2_3211 0.86 +0.8 3,710,971 + Pf6N2E2_3211 0.86 -2.4 3,710,971 + Pf6N2E2_3211 0.86 -1.2 3,710,972 - Pf6N2E2_3211 0.86 -0.1 3,710,972 - Pf6N2E2_3211 0.86 -0.7 3,711,314 + -0.7 3,711,314 + -0.6 3,711,314 + +0.1 3,711,636 - Pf6N2E2_3212 0.13 +0.3 3,711,654 - Pf6N2E2_3212 0.14 -0.1 3,711,702 + Pf6N2E2_3212 0.16 +0.0 3,711,891 - Pf6N2E2_3212 0.24 +0.9 3,711,891 - Pf6N2E2_3212 0.24 +0.6 3,712,198 + Pf6N2E2_3212 0.37 -1.0 3,712,198 + Pf6N2E2_3212 0.37 +1.3 3,712,199 - Pf6N2E2_3212 0.37 +0.0 3,712,218 - Pf6N2E2_3212 0.38 -0.6 3,712,219 + Pf6N2E2_3212 0.38 +0.6 3,712,219 + Pf6N2E2_3212 0.38 -0.2 3,712,219 + Pf6N2E2_3212 0.38 +0.3 3,712,219 + Pf6N2E2_3212 0.38 -0.1 3,712,219 + Pf6N2E2_3212 0.38 +0.1 3,712,219 + Pf6N2E2_3212 0.38 +0.4 3,712,220 - Pf6N2E2_3212 0.38 +0.2 3,712,220 - Pf6N2E2_3212 0.38 +0.6 3,712,220 - Pf6N2E2_3212 0.38 +0.3 3,712,220 - Pf6N2E2_3212 0.38 +0.9 3,712,220 - Pf6N2E2_3212 0.38 -1.2 3,712,223 + Pf6N2E2_3212 0.38 +0.7 3,712,224 - Pf6N2E2_3212 0.38 -0.1
Or see this region's nucleotide sequence