Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_6013

Experiment: sodium fluoride 12.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_6012 and Pf1N1B4_6013 overlap by 4 nucleotidesPf1N1B4_6013 and Pf1N1B4_6014 overlap by 4 nucleotides Pf1N1B4_6012 - COG0419: ATPase involved in DNA repair, at 472,823 to 475,912 _6012 Pf1N1B4_6013 - hypothetical protein, at 475,909 to 476,808 _6013 Pf1N1B4_6014 - ATP-dependent DNA helicase RecQ, at 476,805 to 479,408 _6014 Position (kb) 475 476 477Strain fitness (log2 ratio) -2 -1 0 1 2at 474.960 kb on - strand, within Pf1N1B4_6012at 474.996 kb on - strand, within Pf1N1B4_6012at 475.149 kb on + strand, within Pf1N1B4_6012at 475.150 kb on - strand, within Pf1N1B4_6012at 475.150 kb on - strandat 475.162 kb on + strand, within Pf1N1B4_6012at 475.277 kb on + strand, within Pf1N1B4_6012at 475.309 kb on - strand, within Pf1N1B4_6012at 475.372 kb on + strandat 475.372 kb on + strand, within Pf1N1B4_6012at 475.433 kb on + strand, within Pf1N1B4_6012at 475.462 kb on + strandat 475.503 kb on + strand, within Pf1N1B4_6012at 475.608 kb on + strandat 475.700 kb on + strandat 475.701 kb on - strandat 475.726 kb on + strandat 475.737 kb on + strandat 475.817 kb on + strandat 475.817 kb on + strandat 476.016 kb on + strand, within Pf1N1B4_6013at 476.017 kb on - strand, within Pf1N1B4_6013at 476.017 kb on - strand, within Pf1N1B4_6013at 476.120 kb on - strand, within Pf1N1B4_6013at 476.274 kb on - strand, within Pf1N1B4_6013at 476.334 kb on + strand, within Pf1N1B4_6013at 476.550 kb on + strand, within Pf1N1B4_6013at 476.588 kb on - strand, within Pf1N1B4_6013at 476.682 kb on + strand, within Pf1N1B4_6013at 476.708 kb on - strand, within Pf1N1B4_6013at 476.708 kb on - strand, within Pf1N1B4_6013at 476.709 kb on + strand, within Pf1N1B4_6013at 476.710 kb on - strand, within Pf1N1B4_6013at 476.710 kb on - strand, within Pf1N1B4_6013at 476.710 kb on - strand, within Pf1N1B4_6013at 476.797 kb on + strandat 476.797 kb on + strandat 476.802 kb on + strandat 476.814 kb on + strandat 476.839 kb on + strandat 476.839 kb on + strandat 476.914 kb on - strandat 476.917 kb on - strandat 476.917 kb on - strandat 476.917 kb on - strandat 476.917 kb on - strandat 476.949 kb on + strandat 476.949 kb on + strandat 476.950 kb on - strandat 476.950 kb on - strandat 476.950 kb on - strandat 476.970 kb on - strandat 477.028 kb on - strandat 477.042 kb on + strandat 477.043 kb on - strandat 477.071 kb on - strand, within Pf1N1B4_6014at 477.211 kb on + strand, within Pf1N1B4_6014at 477.220 kb on + strand, within Pf1N1B4_6014at 477.220 kb on + strandat 477.220 kb on + strand, within Pf1N1B4_6014at 477.221 kb on - strand, within Pf1N1B4_6014at 477.414 kb on - strand, within Pf1N1B4_6014at 477.504 kb on + strand, within Pf1N1B4_6014at 477.504 kb on + strand, within Pf1N1B4_6014at 477.504 kb on + strand, within Pf1N1B4_6014at 477.578 kb on + strand, within Pf1N1B4_6014at 477.578 kb on + strandat 477.579 kb on - strand, within Pf1N1B4_6014at 477.579 kb on - strand, within Pf1N1B4_6014at 477.579 kb on - strand, within Pf1N1B4_6014at 477.579 kb on - strand, within Pf1N1B4_6014at 477.579 kb on - strandat 477.579 kb on - strand, within Pf1N1B4_6014at 477.725 kb on + strand, within Pf1N1B4_6014at 477.725 kb on + strand, within Pf1N1B4_6014at 477.726 kb on - strand, within Pf1N1B4_6014at 477.726 kb on - strand, within Pf1N1B4_6014

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Per-strain Table

Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM
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474,960 - Pf1N1B4_6012 0.69 -0.4
474,996 - Pf1N1B4_6012 0.70 +0.5
475,149 + Pf1N1B4_6012 0.75 -0.5
475,150 - Pf1N1B4_6012 0.75 +0.2
475,150 - +1.0
475,162 + Pf1N1B4_6012 0.76 +0.4
475,277 + Pf1N1B4_6012 0.79 -0.6
475,309 - Pf1N1B4_6012 0.80 -1.0
475,372 + -0.0
475,372 + Pf1N1B4_6012 0.82 -0.7
475,433 + Pf1N1B4_6012 0.84 +1.5
475,462 + +0.4
475,503 + Pf1N1B4_6012 0.87 -1.6
475,608 + +1.0
475,700 + -1.0
475,701 - +1.0
475,726 + +0.4
475,737 + +0.1
475,817 + -1.9
475,817 + +0.8
476,016 + Pf1N1B4_6013 0.12 +0.4
476,017 - Pf1N1B4_6013 0.12 -1.5
476,017 - Pf1N1B4_6013 0.12 -0.0
476,120 - Pf1N1B4_6013 0.23 -0.9
476,274 - Pf1N1B4_6013 0.41 -2.2
476,334 + Pf1N1B4_6013 0.47 -2.7
476,550 + Pf1N1B4_6013 0.71 -0.0
476,588 - Pf1N1B4_6013 0.75 +1.0
476,682 + Pf1N1B4_6013 0.86 -1.4
476,708 - Pf1N1B4_6013 0.89 -1.0
476,708 - Pf1N1B4_6013 0.89 -0.3
476,709 + Pf1N1B4_6013 0.89 +0.0
476,710 - Pf1N1B4_6013 0.89 -0.8
476,710 - Pf1N1B4_6013 0.89 +0.6
476,710 - Pf1N1B4_6013 0.89 -1.6
476,797 + -2.0
476,797 + -0.7
476,802 + -0.2
476,814 + -0.6
476,839 + -1.3
476,839 + -1.6
476,914 - -0.4
476,917 - -1.0
476,917 - +1.5
476,917 - -1.0
476,917 - -0.6
476,949 + +2.0
476,949 + -1.6
476,950 - -0.6
476,950 - -0.0
476,950 - +0.7
476,970 - +0.5
477,028 - -1.6
477,042 + +1.0
477,043 - +0.5
477,071 - Pf1N1B4_6014 0.10 -1.4
477,211 + Pf1N1B4_6014 0.16 -1.6
477,220 + Pf1N1B4_6014 0.16 -0.4
477,220 + -0.0
477,220 + Pf1N1B4_6014 0.16 -0.0
477,221 - Pf1N1B4_6014 0.16 +0.3
477,414 - Pf1N1B4_6014 0.23 -0.9
477,504 + Pf1N1B4_6014 0.27 -0.2
477,504 + Pf1N1B4_6014 0.27 +0.6
477,504 + Pf1N1B4_6014 0.27 +1.0
477,578 + Pf1N1B4_6014 0.30 -0.2
477,578 + +0.2
477,579 - Pf1N1B4_6014 0.30 -2.5
477,579 - Pf1N1B4_6014 0.30 -0.6
477,579 - Pf1N1B4_6014 0.30 -0.5
477,579 - Pf1N1B4_6014 0.30 -1.0
477,579 - +0.2
477,579 - Pf1N1B4_6014 0.30 +0.5
477,725 + Pf1N1B4_6014 0.35 +0.3
477,725 + Pf1N1B4_6014 0.35 +0.2
477,726 - Pf1N1B4_6014 0.35 +0.2
477,726 - Pf1N1B4_6014 0.35 -0.2

Or see this region's nucleotide sequence