Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5865 and Pf1N1B4_5866 are separated by 159 nucleotides Pf1N1B4_5866 and Pf1N1B4_5867 are separated by 102 nucleotides Pf1N1B4_5867 and Pf1N1B4_5868 are separated by 105 nucleotides Pf1N1B4_5868 and Pf1N1B4_5869 overlap by 4 nucleotides
Pf1N1B4_5865 - FIG00960493: hypothetical protein, at 300,238 to 301,629
_5865
Pf1N1B4_5866 - ThiJ/PfpI family protein, at 301,789 to 302,466
_5866
Pf1N1B4_5867 - hypothetical protein, at 302,569 to 302,763
_5867
Pf1N1B4_5868 - Secreted protein Hcp, at 302,869 to 303,384
_5868
Pf1N1B4_5869 - hypothetical protein, at 303,381 to 303,497
_5869
Position (kb) 301
302
303 Strain fitness (log2 ratio) -2
-1
0
1
2 at 300.909 kb on + strand, within Pf1N1B4_5865 at 301.234 kb on + strand, within Pf1N1B4_5865 at 301.234 kb on + strand, within Pf1N1B4_5865 at 301.393 kb on + strand, within Pf1N1B4_5865 at 301.394 kb on - strand, within Pf1N1B4_5865 at 301.394 kb on - strand, within Pf1N1B4_5865 at 301.417 kb on + strand, within Pf1N1B4_5865 at 301.418 kb on - strand, within Pf1N1B4_5865 at 301.489 kb on + strand, within Pf1N1B4_5865 at 301.562 kb on - strand at 301.641 kb on + strand at 301.878 kb on - strand, within Pf1N1B4_5866 at 302.186 kb on + strand, within Pf1N1B4_5866 at 302.227 kb on + strand, within Pf1N1B4_5866 at 302.228 kb on - strand, within Pf1N1B4_5866 at 302.372 kb on + strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.376 kb on - strand, within Pf1N1B4_5866 at 302.376 kb on - strand, within Pf1N1B4_5866 at 302.642 kb on - strand, within Pf1N1B4_5867 at 302.776 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.960 kb on - strand, within Pf1N1B4_5868 at 303.059 kb on + strand, within Pf1N1B4_5868 at 303.089 kb on - strand at 303.196 kb on + strand at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.200 kb on - strand, within Pf1N1B4_5868 at 303.338 kb on + strand at 303.339 kb on - strand at 303.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 300,909 + Pf1N1B4_5865 0.48 -0.6 301,234 + Pf1N1B4_5865 0.72 -0.5 301,234 + Pf1N1B4_5865 0.72 -1.6 301,393 + Pf1N1B4_5865 0.83 -0.1 301,394 - Pf1N1B4_5865 0.83 -2.0 301,394 - Pf1N1B4_5865 0.83 +1.8 301,417 + Pf1N1B4_5865 0.85 -2.4 301,418 - Pf1N1B4_5865 0.85 +0.5 301,489 + Pf1N1B4_5865 0.90 -0.6 301,562 - -0.4 301,641 + -0.0 301,878 - Pf1N1B4_5866 0.13 -0.0 302,186 + Pf1N1B4_5866 0.59 -0.4 302,227 + Pf1N1B4_5866 0.65 +1.0 302,228 - Pf1N1B4_5866 0.65 -1.0 302,372 + Pf1N1B4_5866 0.86 -1.6 302,373 - Pf1N1B4_5866 0.86 -0.6 302,373 - Pf1N1B4_5866 0.86 -0.4 302,373 - Pf1N1B4_5866 0.86 -2.4 302,373 - Pf1N1B4_5866 0.86 -0.1 302,376 - Pf1N1B4_5866 0.87 -0.9 302,376 - Pf1N1B4_5866 0.87 -0.8 302,642 - Pf1N1B4_5867 0.37 +0.8 302,776 + +0.2 302,884 + -0.4 302,884 + -1.4 302,884 + -1.0 302,884 + -2.4 302,884 + +1.0 302,884 + -1.4 302,884 + +1.0 302,884 + +1.0 302,885 - -1.4 302,885 - -1.4 302,885 - -0.8 302,885 - -0.6 302,885 - -0.9 302,885 - -0.4 302,885 - +0.6 302,885 - +0.3 302,885 - +0.2 302,960 - Pf1N1B4_5868 0.18 -0.3 303,059 + Pf1N1B4_5868 0.37 +0.5 303,089 - +1.0 303,196 + -0.6 303,197 - Pf1N1B4_5868 0.64 -0.9 303,197 - Pf1N1B4_5868 0.64 -0.0 303,197 - Pf1N1B4_5868 0.64 -0.1 303,199 + Pf1N1B4_5868 0.64 +0.1 303,199 + Pf1N1B4_5868 0.64 -0.5 303,199 + +0.2 303,199 + Pf1N1B4_5868 0.64 +0.8 303,199 + Pf1N1B4_5868 0.64 -0.5 303,200 - Pf1N1B4_5868 0.64 -0.0 303,338 + -1.4 303,339 - +0.3 303,383 - -0.3
Or see this region's nucleotide sequence