Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5786 and Pf1N1B4_5787 are separated by 219 nucleotides Pf1N1B4_5787 and Pf1N1B4_5788 are separated by 413 nucleotides Pf1N1B4_5788 and Pf1N1B4_5789 are separated by 188 nucleotides
Pf1N1B4_5786 - putative membrane protein, at 225,003 to 225,446
_5786
Pf1N1B4_5787 - Gifsy-2 prophage protein, at 225,666 to 226,358
_5787
Pf1N1B4_5788 - CBS domain protein, at 226,772 to 226,915
_5788
Pf1N1B4_5789 - hypothetical protein, at 227,104 to 227,238
_5789
Position (kb) 225
226
227 Strain fitness (log2 ratio) -2
-1
0
1
2 at 224.781 kb on + strand at 224.781 kb on + strand at 224.953 kb on + strand at 224.953 kb on + strand at 224.953 kb on + strand at 224.954 kb on - strand at 225.016 kb on + strand at 225.016 kb on + strand at 225.017 kb on - strand at 225.226 kb on + strand at 225.330 kb on + strand, within Pf1N1B4_5786 at 225.331 kb on - strand, within Pf1N1B4_5786 at 225.331 kb on - strand at 225.331 kb on - strand, within Pf1N1B4_5786 at 225.331 kb on - strand, within Pf1N1B4_5786 at 225.628 kb on + strand at 225.636 kb on - strand at 225.636 kb on - strand at 225.641 kb on + strand at 225.641 kb on + strand at 225.743 kb on + strand, within Pf1N1B4_5787 at 225.772 kb on - strand, within Pf1N1B4_5787 at 225.772 kb on - strand, within Pf1N1B4_5787 at 225.937 kb on + strand, within Pf1N1B4_5787 at 225.938 kb on - strand, within Pf1N1B4_5787 at 225.956 kb on + strand, within Pf1N1B4_5787 at 225.957 kb on - strand at 225.957 kb on - strand, within Pf1N1B4_5787 at 225.957 kb on - strand, within Pf1N1B4_5787 at 225.957 kb on - strand, within Pf1N1B4_5787 at 225.988 kb on - strand, within Pf1N1B4_5787 at 226.015 kb on - strand at 226.078 kb on - strand, within Pf1N1B4_5787 at 226.237 kb on + strand, within Pf1N1B4_5787 at 226.237 kb on + strand, within Pf1N1B4_5787 at 226.238 kb on - strand, within Pf1N1B4_5787 at 226.487 kb on + strand at 226.607 kb on - strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.656 kb on + strand at 226.657 kb on - strand at 226.657 kb on - strand at 226.657 kb on - strand at 226.657 kb on - strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.660 kb on + strand at 226.710 kb on + strand at 226.710 kb on + strand at 226.710 kb on + strand at 226.711 kb on - strand at 226.752 kb on + strand at 226.752 kb on + strand at 226.752 kb on + strand at 226.752 kb on + strand at 226.753 kb on - strand at 226.753 kb on - strand at 226.937 kb on + strand at 226.954 kb on + strand at 227.219 kb on + strand at 227.220 kb on - strand, within Pf1N1B4_5789 at 227.268 kb on - strand at 227.331 kb on - strand at 227.331 kb on - strand at 227.331 kb on - strand at 227.335 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 224,781 + -0.7 224,781 + -1.3 224,953 + +1.9 224,953 + -2.7 224,953 + -0.1 224,954 - +0.2 225,016 + +0.0 225,016 + +0.0 225,017 - -0.1 225,226 + +2.5 225,330 + Pf1N1B4_5786 0.74 -0.3 225,331 - Pf1N1B4_5786 0.74 -0.6 225,331 - -1.4 225,331 - Pf1N1B4_5786 0.74 +1.7 225,331 - Pf1N1B4_5786 0.74 -0.4 225,628 + +0.6 225,636 - +0.9 225,636 - -0.4 225,641 + +0.4 225,641 + -1.1 225,743 + Pf1N1B4_5787 0.11 -0.3 225,772 - Pf1N1B4_5787 0.15 -0.1 225,772 - Pf1N1B4_5787 0.15 -1.1 225,937 + Pf1N1B4_5787 0.39 +0.1 225,938 - Pf1N1B4_5787 0.39 -0.1 225,956 + Pf1N1B4_5787 0.42 -1.1 225,957 - -1.4 225,957 - Pf1N1B4_5787 0.42 -1.7 225,957 - Pf1N1B4_5787 0.42 +0.1 225,957 - Pf1N1B4_5787 0.42 -2.1 225,988 - Pf1N1B4_5787 0.46 -0.1 226,015 - -1.1 226,078 - Pf1N1B4_5787 0.59 -0.2 226,237 + Pf1N1B4_5787 0.82 -0.7 226,237 + Pf1N1B4_5787 0.82 -2.3 226,238 - Pf1N1B4_5787 0.83 -1.1 226,487 + -0.5 226,607 - -2.1 226,656 + -0.7 226,656 + +1.5 226,656 + +0.6 226,656 + -2.0 226,656 + +1.5 226,656 + -1.1 226,656 + -1.4 226,656 + +0.5 226,656 + -0.9 226,656 + -0.1 226,656 + +1.3 226,657 - -0.0 226,657 - +0.9 226,657 - -0.1 226,657 - +0.9 226,660 + -1.2 226,660 + +0.9 226,660 + +0.3 226,660 + +1.1 226,660 + +2.2 226,660 + -0.5 226,660 + +0.2 226,660 + +0.4 226,660 + -0.0 226,660 + -0.7 226,660 + -1.4 226,710 + +0.9 226,710 + +0.9 226,710 + -0.5 226,711 - +1.1 226,752 + +1.1 226,752 + +1.3 226,752 + +0.3 226,752 + +0.1 226,753 - -2.3 226,753 - +0.3 226,937 + +0.9 226,954 + +0.9 227,219 + -0.4 227,220 - Pf1N1B4_5789 0.86 +1.9 227,268 - -0.1 227,331 - +0.9 227,331 - +1.2 227,331 - -0.7 227,335 - -0.3
Or see this region's nucleotide sequence