Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5705 and Pf1N1B4_5706 overlap by 1 nucleotides Pf1N1B4_5706 and Pf1N1B4_5707 are separated by 10 nucleotides
Pf1N1B4_5705 - Acetyl-coenzyme A synthetase (EC 6.2.1.1), at 142,638 to 144,539
_5705
Pf1N1B4_5706 - Fumarylacetoacetate hydrolase family protein, at 144,539 to 145,660
_5706
Pf1N1B4_5707 - NADH dehydrogenase (EC 1.6.99.3), at 145,671 to 146,762
_5707
Position (kb) 144
145
146 Strain fitness (log2 ratio) -2
-1
0
1
2 at 143.875 kb on + strand, within Pf1N1B4_5705 at 143.876 kb on - strand, within Pf1N1B4_5705 at 143.979 kb on + strand, within Pf1N1B4_5705 at 144.037 kb on + strand at 144.038 kb on - strand, within Pf1N1B4_5705 at 144.038 kb on - strand, within Pf1N1B4_5705 at 144.180 kb on + strand at 144.181 kb on - strand, within Pf1N1B4_5705 at 144.278 kb on + strand, within Pf1N1B4_5705 at 144.279 kb on - strand, within Pf1N1B4_5705 at 144.279 kb on - strand, within Pf1N1B4_5705 at 144.303 kb on + strand, within Pf1N1B4_5705 at 144.304 kb on - strand, within Pf1N1B4_5705 at 144.304 kb on - strand, within Pf1N1B4_5705 at 144.362 kb on - strand at 144.362 kb on - strand at 144.373 kb on + strand at 144.373 kb on + strand at 144.373 kb on + strand at 144.460 kb on + strand at 144.461 kb on - strand at 144.465 kb on - strand at 144.637 kb on - strand at 144.884 kb on + strand, within Pf1N1B4_5706 at 144.885 kb on - strand, within Pf1N1B4_5706 at 144.910 kb on + strand, within Pf1N1B4_5706 at 144.978 kb on + strand, within Pf1N1B4_5706 at 145.436 kb on + strand, within Pf1N1B4_5706 at 145.479 kb on - strand, within Pf1N1B4_5706 at 145.479 kb on - strand, within Pf1N1B4_5706 at 145.484 kb on + strand, within Pf1N1B4_5706 at 145.502 kb on + strand, within Pf1N1B4_5706 at 145.639 kb on - strand at 145.847 kb on + strand, within Pf1N1B4_5707 at 146.047 kb on + strand, within Pf1N1B4_5707 at 146.048 kb on - strand, within Pf1N1B4_5707 at 146.048 kb on - strand, within Pf1N1B4_5707 at 146.048 kb on - strand at 146.082 kb on - strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.132 kb on + strand, within Pf1N1B4_5707 at 146.133 kb on - strand, within Pf1N1B4_5707 at 146.133 kb on - strand, within Pf1N1B4_5707 at 146.151 kb on + strand, within Pf1N1B4_5707 at 146.152 kb on - strand, within Pf1N1B4_5707 at 146.190 kb on - strand, within Pf1N1B4_5707 at 146.348 kb on + strand, within Pf1N1B4_5707 at 146.348 kb on + strand, within Pf1N1B4_5707 at 146.349 kb on - strand at 146.549 kb on - strand at 146.555 kb on - strand, within Pf1N1B4_5707 at 146.555 kb on - strand, within Pf1N1B4_5707 at 146.582 kb on - strand, within Pf1N1B4_5707
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 143,875 + Pf1N1B4_5705 0.65 -0.7 143,876 - Pf1N1B4_5705 0.65 +0.7 143,979 + Pf1N1B4_5705 0.71 +0.1 144,037 + -0.1 144,038 - Pf1N1B4_5705 0.74 +0.6 144,038 - Pf1N1B4_5705 0.74 -0.7 144,180 + -0.7 144,181 - Pf1N1B4_5705 0.81 +0.2 144,278 + Pf1N1B4_5705 0.86 -1.4 144,279 - Pf1N1B4_5705 0.86 +0.1 144,279 - Pf1N1B4_5705 0.86 +0.6 144,303 + Pf1N1B4_5705 0.88 -0.1 144,304 - Pf1N1B4_5705 0.88 +0.1 144,304 - Pf1N1B4_5705 0.88 -0.1 144,362 - +0.9 144,362 - +0.7 144,373 + +1.9 144,373 + -0.1 144,373 + -1.2 144,460 + -1.4 144,461 - -0.7 144,465 - +0.4 144,637 - -0.7 144,884 + Pf1N1B4_5706 0.31 -2.6 144,885 - Pf1N1B4_5706 0.31 -0.1 144,910 + Pf1N1B4_5706 0.33 -0.1 144,978 + Pf1N1B4_5706 0.39 -1.5 145,436 + Pf1N1B4_5706 0.80 -0.5 145,479 - Pf1N1B4_5706 0.84 -1.1 145,479 - Pf1N1B4_5706 0.84 -0.8 145,484 + Pf1N1B4_5706 0.84 -0.4 145,502 + Pf1N1B4_5706 0.86 -0.8 145,639 - -0.1 145,847 + Pf1N1B4_5707 0.16 -0.9 146,047 + Pf1N1B4_5707 0.34 -0.8 146,048 - Pf1N1B4_5707 0.35 -0.1 146,048 - Pf1N1B4_5707 0.35 -1.0 146,048 - -1.4 146,082 - Pf1N1B4_5707 0.38 -0.4 146,132 + Pf1N1B4_5707 0.42 +1.9 146,132 + Pf1N1B4_5707 0.42 -1.1 146,132 + Pf1N1B4_5707 0.42 -1.4 146,133 - Pf1N1B4_5707 0.42 +0.1 146,133 - Pf1N1B4_5707 0.42 -0.7 146,151 + Pf1N1B4_5707 0.44 +0.5 146,152 - Pf1N1B4_5707 0.44 +0.9 146,190 - Pf1N1B4_5707 0.48 +0.9 146,348 + Pf1N1B4_5707 0.62 -0.1 146,348 + Pf1N1B4_5707 0.62 -0.7 146,349 - +0.9 146,549 - -1.4 146,555 - Pf1N1B4_5707 0.81 +0.3 146,555 - Pf1N1B4_5707 0.81 +0.9 146,582 - Pf1N1B4_5707 0.83 +0.9
Or see this region's nucleotide sequence