Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5701

Experiment: sodium fluoride 12.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5700 and Pf1N1B4_5701 are separated by 169 nucleotidesPf1N1B4_5701 and Pf1N1B4_5702 overlap by 14 nucleotidesPf1N1B4_5702 and Pf1N1B4_5703 are separated by 42 nucleotidesPf1N1B4_5703 and Pf1N1B4_5704 are separated by 82 nucleotides Pf1N1B4_5700 - Putative tRNA-m1A22 methylase, at 139,167 to 139,841 _5700 Pf1N1B4_5701 - FIG00955602: hypothetical protein, at 140,011 to 140,172 _5701 Pf1N1B4_5702 - hypothetical protein, at 140,159 to 140,320 _5702 Pf1N1B4_5703 - FIG00961921: hypothetical protein, at 140,363 to 141,073 _5703 Pf1N1B4_5704 - Long-chain fatty acid transport protein, at 141,156 to 142,475 _5704 Position (kb) 140 141Strain fitness (log2 ratio) -2 -1 0 1at 139.058 kb on + strandat 139.065 kb on - strandat 139.096 kb on - strandat 139.096 kb on - strandat 139.144 kb on + strandat 139.346 kb on - strand, within Pf1N1B4_5700at 139.363 kb on + strand, within Pf1N1B4_5700at 139.363 kb on + strand, within Pf1N1B4_5700at 139.363 kb on + strand, within Pf1N1B4_5700at 139.364 kb on - strand, within Pf1N1B4_5700at 139.364 kb on - strand, within Pf1N1B4_5700at 139.364 kb on - strand, within Pf1N1B4_5700at 139.403 kb on - strand, within Pf1N1B4_5700at 139.406 kb on - strand, within Pf1N1B4_5700at 139.679 kb on + strand, within Pf1N1B4_5700at 139.679 kb on + strand, within Pf1N1B4_5700at 139.812 kb on + strandat 139.812 kb on + strandat 139.812 kb on + strandat 139.813 kb on - strandat 139.813 kb on - strandat 139.876 kb on + strandat 139.877 kb on - strandat 140.028 kb on + strand, within Pf1N1B4_5701at 140.076 kb on + strand, within Pf1N1B4_5701at 140.077 kb on - strand, within Pf1N1B4_5701at 140.077 kb on - strandat 140.077 kb on - strand, within Pf1N1B4_5701at 140.085 kb on - strandat 140.088 kb on + strand, within Pf1N1B4_5701at 140.089 kb on - strand, within Pf1N1B4_5701at 140.095 kb on - strand, within Pf1N1B4_5701at 140.095 kb on - strand, within Pf1N1B4_5701at 140.141 kb on - strand, within Pf1N1B4_5701at 140.143 kb on + strand, within Pf1N1B4_5701at 140.143 kb on + strand, within Pf1N1B4_5701at 140.172 kb on + strandat 140.172 kb on + strandat 140.328 kb on - strandat 140.339 kb on + strandat 140.654 kb on + strand, within Pf1N1B4_5703at 140.654 kb on + strand, within Pf1N1B4_5703at 140.654 kb on + strand, within Pf1N1B4_5703at 140.654 kb on + strand, within Pf1N1B4_5703at 140.655 kb on - strand, within Pf1N1B4_5703at 140.696 kb on + strand, within Pf1N1B4_5703at 140.835 kb on - strand, within Pf1N1B4_5703at 141.049 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM
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139,058 + +0.9
139,065 - +0.9
139,096 - +0.2
139,096 - -0.1
139,144 + -0.4
139,346 - Pf1N1B4_5700 0.27 -0.1
139,363 + Pf1N1B4_5700 0.29 -0.9
139,363 + Pf1N1B4_5700 0.29 -0.4
139,363 + Pf1N1B4_5700 0.29 +0.0
139,364 - Pf1N1B4_5700 0.29 -0.2
139,364 - Pf1N1B4_5700 0.29 -0.1
139,364 - Pf1N1B4_5700 0.29 -1.1
139,403 - Pf1N1B4_5700 0.35 -0.2
139,406 - Pf1N1B4_5700 0.35 +0.4
139,679 + Pf1N1B4_5700 0.76 +0.9
139,679 + Pf1N1B4_5700 0.76 -1.7
139,812 + -1.3
139,812 + -0.8
139,812 + +0.3
139,813 - -0.1
139,813 - +0.9
139,876 + +0.3
139,877 - +0.3
140,028 + Pf1N1B4_5701 0.10 -0.5
140,076 + Pf1N1B4_5701 0.40 -1.3
140,077 - Pf1N1B4_5701 0.41 -1.7
140,077 - -0.1
140,077 - Pf1N1B4_5701 0.41 -0.2
140,085 - -0.7
140,088 + Pf1N1B4_5701 0.48 -1.7
140,089 - Pf1N1B4_5701 0.48 +0.5
140,095 - Pf1N1B4_5701 0.52 -0.3
140,095 - Pf1N1B4_5701 0.52 -0.1
140,141 - Pf1N1B4_5701 0.80 -1.9
140,143 + Pf1N1B4_5701 0.81 -0.7
140,143 + Pf1N1B4_5701 0.81 +0.9
140,172 + +0.4
140,172 + +0.1
140,328 - +0.6
140,339 + +0.5
140,654 + Pf1N1B4_5703 0.41 +0.2
140,654 + Pf1N1B4_5703 0.41 +0.3
140,654 + Pf1N1B4_5703 0.41 +0.7
140,654 + Pf1N1B4_5703 0.41 +0.1
140,655 - Pf1N1B4_5703 0.41 -0.9
140,696 + Pf1N1B4_5703 0.47 -0.1
140,835 - Pf1N1B4_5703 0.66 -0.3
141,049 - +1.2

Or see this region's nucleotide sequence