Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5498 and Pf1N1B4_5499 are separated by 367 nucleotides
Pf1N1B4_5498 - hypothetical protein, at 247,310 to 247,522
_5498
Pf1N1B4_5499 - hypothetical protein, at 247,890 to 249,695
_5499
Position (kb) 247
248 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 246.375 kb on + strand at 246.375 kb on + strand at 246.376 kb on - strand at 246.537 kb on - strand at 246.810 kb on + strand at 246.884 kb on - strand at 246.884 kb on - strand at 247.038 kb on + strand at 247.038 kb on + strand at 247.041 kb on - strand at 247.041 kb on - strand at 247.109 kb on + strand at 247.150 kb on - strand at 247.191 kb on + strand at 247.192 kb on - strand at 247.219 kb on + strand at 247.252 kb on + strand at 247.252 kb on + strand at 247.253 kb on - strand at 247.253 kb on - strand at 247.255 kb on - strand at 247.361 kb on + strand, within Pf1N1B4_5498 at 247.362 kb on - strand, within Pf1N1B4_5498 at 247.362 kb on - strand, within Pf1N1B4_5498 at 247.462 kb on + strand, within Pf1N1B4_5498 at 247.462 kb on + strand, within Pf1N1B4_5498 at 247.631 kb on + strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.632 kb on - strand at 247.683 kb on + strand at 247.684 kb on - strand at 247.705 kb on + strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.706 kb on - strand at 247.780 kb on + strand at 247.780 kb on + strand at 247.780 kb on + strand at 247.781 kb on - strand at 247.781 kb on - strand at 247.855 kb on + strand at 248.092 kb on + strand, within Pf1N1B4_5499 at 248.092 kb on + strand, within Pf1N1B4_5499 at 248.093 kb on - strand at 248.320 kb on + strand, within Pf1N1B4_5499 at 248.356 kb on + strand at 248.356 kb on + strand, within Pf1N1B4_5499 at 248.356 kb on + strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand, within Pf1N1B4_5499 at 248.357 kb on - strand at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.434 kb on + strand, within Pf1N1B4_5499 at 248.435 kb on - strand, within Pf1N1B4_5499 at 248.435 kb on - strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.515 kb on + strand, within Pf1N1B4_5499 at 248.516 kb on - strand, within Pf1N1B4_5499 at 248.516 kb on - strand, within Pf1N1B4_5499
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 246,375 + -1.0 246,375 + -0.9 246,376 - -1.2 246,537 - +0.7 246,810 + +1.1 246,884 - +1.0 246,884 - -0.4 247,038 + +0.2 247,038 + -1.4 247,041 - +1.4 247,041 - -0.2 247,109 + +1.1 247,150 - -0.2 247,191 + -0.9 247,192 - +0.6 247,219 + -1.4 247,252 + +3.0 247,252 + +0.8 247,253 - -0.9 247,253 - -1.4 247,255 - -0.0 247,361 + Pf1N1B4_5498 0.24 +0.2 247,362 - Pf1N1B4_5498 0.24 +0.2 247,362 - Pf1N1B4_5498 0.24 -1.2 247,462 + Pf1N1B4_5498 0.71 -1.4 247,462 + Pf1N1B4_5498 0.71 -0.6 247,631 + -2.1 247,632 - +0.7 247,632 - -1.6 247,632 - -0.9 247,632 - +2.4 247,683 + -0.2 247,684 - -0.2 247,705 + -0.3 247,706 - -1.1 247,706 - +1.4 247,706 - +0.7 247,706 - -0.5 247,780 + -0.4 247,780 + +0.4 247,780 + +1.4 247,781 - -1.3 247,781 - +0.2 247,855 + +1.0 248,092 + Pf1N1B4_5499 0.11 +0.2 248,092 + Pf1N1B4_5499 0.11 +0.6 248,093 - +2.4 248,320 + Pf1N1B4_5499 0.24 -1.1 248,356 + +2.4 248,356 + Pf1N1B4_5499 0.26 -0.5 248,356 + Pf1N1B4_5499 0.26 +0.4 248,357 - Pf1N1B4_5499 0.26 -2.2 248,357 - Pf1N1B4_5499 0.26 -0.4 248,357 - Pf1N1B4_5499 0.26 -1.0 248,357 - Pf1N1B4_5499 0.26 +0.2 248,357 - -0.6 248,434 + Pf1N1B4_5499 0.30 +2.4 248,434 + Pf1N1B4_5499 0.30 -0.5 248,434 + Pf1N1B4_5499 0.30 +0.1 248,434 + Pf1N1B4_5499 0.30 +0.1 248,434 + Pf1N1B4_5499 0.30 +0.2 248,434 + Pf1N1B4_5499 0.30 -1.2 248,435 - Pf1N1B4_5499 0.30 +0.8 248,435 - Pf1N1B4_5499 0.30 -0.4 248,515 + Pf1N1B4_5499 0.35 -1.2 248,515 + Pf1N1B4_5499 0.35 +0.4 248,515 + Pf1N1B4_5499 0.35 -0.6 248,516 - Pf1N1B4_5499 0.35 +0.4 248,516 - Pf1N1B4_5499 0.35 -0.1
Or see this region's nucleotide sequence