Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4654 and Pf1N1B4_4655 are separated by 15 nucleotides Pf1N1B4_4655 and Pf1N1B4_4656 are separated by 213 nucleotides Pf1N1B4_4656 and Pf1N1B4_4657 overlap by 4 nucleotides Pf1N1B4_4657 and Pf1N1B4_4658 are separated by 227 nucleotides
Pf1N1B4_4654 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 5,132,041 to 5,133,507
_4654
Pf1N1B4_4655 - hypothetical protein, at 5,133,523 to 5,133,636
_4655
Pf1N1B4_4656 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 5,133,850 to 5,135,523
_4656
Pf1N1B4_4657 - Oxidoreductase probably involved in sulfite reduction, at 5,135,520 to 5,136,014
_4657
Pf1N1B4_4658 - hypothetical protein, at 5,136,242 to 5,137,210
_4658
Position (kb) 5133
5134
5135
5136 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5132.853 kb on - strand, within Pf1N1B4_4654 at 5132.888 kb on + strand, within Pf1N1B4_4654 at 5132.993 kb on + strand, within Pf1N1B4_4654 at 5132.993 kb on + strand, within Pf1N1B4_4654 at 5133.230 kb on + strand, within Pf1N1B4_4654 at 5133.233 kb on + strand, within Pf1N1B4_4654 at 5133.233 kb on + strand, within Pf1N1B4_4654 at 5133.233 kb on + strand, within Pf1N1B4_4654 at 5133.402 kb on + strand at 5133.403 kb on - strand at 5133.403 kb on - strand at 5133.434 kb on + strand at 5133.434 kb on + strand at 5133.434 kb on + strand at 5133.435 kb on - strand at 5133.435 kb on - strand at 5133.438 kb on + strand at 5133.440 kb on + strand at 5133.440 kb on + strand at 5133.441 kb on - strand at 5133.441 kb on - strand at 5133.441 kb on - strand at 5133.532 kb on - strand at 5133.676 kb on + strand at 5133.676 kb on + strand at 5133.676 kb on + strand at 5133.676 kb on + strand at 5133.676 kb on + strand at 5133.853 kb on + strand at 5133.853 kb on + strand at 5133.853 kb on + strand at 5133.853 kb on + strand at 5133.853 kb on + strand at 5133.854 kb on - strand at 5133.854 kb on - strand at 5133.854 kb on - strand at 5133.854 kb on - strand at 5133.859 kb on + strand at 5133.869 kb on - strand at 5133.985 kb on + strand at 5133.985 kb on + strand at 5133.986 kb on - strand at 5133.986 kb on - strand at 5134.003 kb on + strand at 5134.004 kb on - strand at 5134.081 kb on + strand, within Pf1N1B4_4656 at 5134.082 kb on - strand, within Pf1N1B4_4656 at 5134.411 kb on + strand, within Pf1N1B4_4656 at 5134.418 kb on - strand, within Pf1N1B4_4656 at 5134.558 kb on + strand, within Pf1N1B4_4656 at 5134.559 kb on - strand, within Pf1N1B4_4656 at 5134.588 kb on + strand, within Pf1N1B4_4656 at 5135.183 kb on - strand, within Pf1N1B4_4656 at 5135.215 kb on - strand, within Pf1N1B4_4656 at 5135.451 kb on + strand at 5135.452 kb on - strand at 5135.787 kb on + strand, within Pf1N1B4_4657 at 5135.799 kb on + strand, within Pf1N1B4_4657 at 5135.854 kb on + strand, within Pf1N1B4_4657 at 5135.854 kb on + strand, within Pf1N1B4_4657 at 5135.854 kb on + strand, within Pf1N1B4_4657 at 5135.854 kb on + strand, within Pf1N1B4_4657 at 5135.855 kb on - strand, within Pf1N1B4_4657 at 5135.919 kb on - strand, within Pf1N1B4_4657 at 5135.919 kb on - strand, within Pf1N1B4_4657 at 5136.213 kb on - strand at 5136.315 kb on - strand at 5136.366 kb on - strand, within Pf1N1B4_4658
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 5,132,853 - Pf1N1B4_4654 0.55 -1.0 5,132,888 + Pf1N1B4_4654 0.58 -0.4 5,132,993 + Pf1N1B4_4654 0.65 -0.4 5,132,993 + Pf1N1B4_4654 0.65 -0.5 5,133,230 + Pf1N1B4_4654 0.81 -1.1 5,133,233 + Pf1N1B4_4654 0.81 -0.4 5,133,233 + Pf1N1B4_4654 0.81 +1.5 5,133,233 + Pf1N1B4_4654 0.81 +0.3 5,133,402 + -1.1 5,133,403 - -0.1 5,133,403 - +0.1 5,133,434 + -1.7 5,133,434 + +0.2 5,133,434 + -0.5 5,133,435 - +1.5 5,133,435 - +0.9 5,133,438 + -0.5 5,133,440 + -0.1 5,133,440 + +0.5 5,133,441 - +0.7 5,133,441 - +0.0 5,133,441 - -1.1 5,133,532 - +0.1 5,133,676 + +0.4 5,133,676 + +0.5 5,133,676 + +1.6 5,133,676 + -1.8 5,133,676 + -0.4 5,133,853 + -0.6 5,133,853 + +1.5 5,133,853 + -0.5 5,133,853 + +0.6 5,133,853 + +1.9 5,133,854 - -0.1 5,133,854 - -1.1 5,133,854 - +0.1 5,133,854 - +1.5 5,133,859 + -1.5 5,133,869 - -0.1 5,133,985 + -0.1 5,133,985 + -1.0 5,133,986 - +0.2 5,133,986 - +0.9 5,134,003 + +0.5 5,134,004 - +1.9 5,134,081 + Pf1N1B4_4656 0.14 -0.9 5,134,082 - Pf1N1B4_4656 0.14 -2.8 5,134,411 + Pf1N1B4_4656 0.34 -0.8 5,134,418 - Pf1N1B4_4656 0.34 -0.8 5,134,558 + Pf1N1B4_4656 0.42 -1.9 5,134,559 - Pf1N1B4_4656 0.42 -1.7 5,134,588 + Pf1N1B4_4656 0.44 -0.4 5,135,183 - Pf1N1B4_4656 0.80 -1.5 5,135,215 - Pf1N1B4_4656 0.82 -2.0 5,135,451 + -0.7 5,135,452 - +1.9 5,135,787 + Pf1N1B4_4657 0.54 -0.9 5,135,799 + Pf1N1B4_4657 0.56 -1.5 5,135,854 + Pf1N1B4_4657 0.67 +1.5 5,135,854 + Pf1N1B4_4657 0.67 +0.9 5,135,854 + Pf1N1B4_4657 0.67 +0.1 5,135,854 + Pf1N1B4_4657 0.67 +0.3 5,135,855 - Pf1N1B4_4657 0.68 -2.0 5,135,919 - Pf1N1B4_4657 0.81 +1.5 5,135,919 - Pf1N1B4_4657 0.81 -0.7 5,136,213 - -0.7 5,136,315 - +0.9 5,136,366 - Pf1N1B4_4658 0.13 +0.1
Or see this region's nucleotide sequence