Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4326

Experiment: sodium fluoride 12.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4325 and Pf1N1B4_4326 are separated by 4 nucleotidesPf1N1B4_4326 and Pf1N1B4_4327 overlap by 29 nucleotides Pf1N1B4_4325 - Agmatine deiminase (EC 3.5.3.12), at 4,785,715 to 4,786,767 _4325 Pf1N1B4_4326 - N-carbamoylputrescine amidase (3.5.1.53), at 4,786,772 to 4,787,680 _4326 Pf1N1B4_4327 - Spermidine/putrescine-binding periplasmic protein, at 4,787,652 to 4,788,785 _4327 Position (kb) 4786 4787 4788Strain fitness (log2 ratio) -2 -1 0 1 2at 4786.150 kb on - strand, within Pf1N1B4_4325at 4786.162 kb on - strand, within Pf1N1B4_4325at 4786.335 kb on - strand, within Pf1N1B4_4325at 4786.335 kb on - strand, within Pf1N1B4_4325at 4786.433 kb on - strand, within Pf1N1B4_4325at 4786.516 kb on + strand, within Pf1N1B4_4325at 4786.516 kb on + strand, within Pf1N1B4_4325at 4786.517 kb on - strand, within Pf1N1B4_4325at 4786.517 kb on - strandat 4786.579 kb on + strand, within Pf1N1B4_4325at 4786.917 kb on - strand, within Pf1N1B4_4326at 4786.917 kb on - strand, within Pf1N1B4_4326at 4786.917 kb on - strand, within Pf1N1B4_4326at 4786.980 kb on + strandat 4787.061 kb on - strand, within Pf1N1B4_4326at 4787.159 kb on + strand, within Pf1N1B4_4326at 4787.160 kb on - strand, within Pf1N1B4_4326at 4787.462 kb on + strand, within Pf1N1B4_4326at 4787.616 kb on - strandat 4787.616 kb on - strandat 4787.779 kb on - strand, within Pf1N1B4_4327at 4787.794 kb on - strand, within Pf1N1B4_4327at 4787.850 kb on + strand, within Pf1N1B4_4327at 4787.850 kb on + strand, within Pf1N1B4_4327at 4787.850 kb on + strand, within Pf1N1B4_4327at 4787.850 kb on + strand, within Pf1N1B4_4327at 4788.042 kb on + strand, within Pf1N1B4_4327at 4788.042 kb on + strand, within Pf1N1B4_4327at 4788.042 kb on + strand, within Pf1N1B4_4327at 4788.042 kb on + strand, within Pf1N1B4_4327at 4788.043 kb on - strandat 4788.043 kb on - strandat 4788.072 kb on + strand, within Pf1N1B4_4327at 4788.073 kb on - strand, within Pf1N1B4_4327at 4788.099 kb on + strandat 4788.201 kb on - strand, within Pf1N1B4_4327at 4788.201 kb on - strand, within Pf1N1B4_4327at 4788.249 kb on + strand, within Pf1N1B4_4327at 4788.250 kb on - strand, within Pf1N1B4_4327at 4788.320 kb on - strand, within Pf1N1B4_4327at 4788.327 kb on + strand, within Pf1N1B4_4327at 4788.332 kb on - strand, within Pf1N1B4_4327at 4788.543 kb on + strand, within Pf1N1B4_4327at 4788.544 kb on - strand, within Pf1N1B4_4327at 4788.672 kb on + strandat 4788.672 kb on + strandat 4788.673 kb on - strandat 4788.673 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM
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4,786,150 - Pf1N1B4_4325 0.41 +0.5
4,786,162 - Pf1N1B4_4325 0.42 +0.5
4,786,335 - Pf1N1B4_4325 0.59 -0.8
4,786,335 - Pf1N1B4_4325 0.59 +1.7
4,786,433 - Pf1N1B4_4325 0.68 +0.2
4,786,516 + Pf1N1B4_4325 0.76 -1.5
4,786,516 + Pf1N1B4_4325 0.76 +0.5
4,786,517 - Pf1N1B4_4325 0.76 +0.8
4,786,517 - +0.6
4,786,579 + Pf1N1B4_4325 0.82 -0.4
4,786,917 - Pf1N1B4_4326 0.16 -2.0
4,786,917 - Pf1N1B4_4326 0.16 -1.4
4,786,917 - Pf1N1B4_4326 0.16 -2.2
4,786,980 + +0.2
4,787,061 - Pf1N1B4_4326 0.32 -1.0
4,787,159 + Pf1N1B4_4326 0.43 +1.4
4,787,160 - Pf1N1B4_4326 0.43 +0.7
4,787,462 + Pf1N1B4_4326 0.76 -1.4
4,787,616 - -0.5
4,787,616 - -0.3
4,787,779 - Pf1N1B4_4327 0.11 +0.2
4,787,794 - Pf1N1B4_4327 0.13 -1.7
4,787,850 + Pf1N1B4_4327 0.17 -1.1
4,787,850 + Pf1N1B4_4327 0.17 -1.0
4,787,850 + Pf1N1B4_4327 0.17 -0.1
4,787,850 + Pf1N1B4_4327 0.17 -0.2
4,788,042 + Pf1N1B4_4327 0.34 +0.2
4,788,042 + Pf1N1B4_4327 0.34 -0.3
4,788,042 + Pf1N1B4_4327 0.34 +0.9
4,788,042 + Pf1N1B4_4327 0.34 -1.1
4,788,043 - -1.5
4,788,043 - +1.9
4,788,072 + Pf1N1B4_4327 0.37 -0.4
4,788,073 - Pf1N1B4_4327 0.37 -0.9
4,788,099 + -0.1
4,788,201 - Pf1N1B4_4327 0.48 +1.7
4,788,201 - Pf1N1B4_4327 0.48 +0.5
4,788,249 + Pf1N1B4_4327 0.53 -0.1
4,788,250 - Pf1N1B4_4327 0.53 -0.5
4,788,320 - Pf1N1B4_4327 0.59 -1.1
4,788,327 + Pf1N1B4_4327 0.60 -0.6
4,788,332 - Pf1N1B4_4327 0.60 +0.3
4,788,543 + Pf1N1B4_4327 0.79 -0.7
4,788,544 - Pf1N1B4_4327 0.79 -0.0
4,788,672 + -0.9
4,788,672 + -1.1
4,788,673 - +0.2
4,788,673 - +1.3

Or see this region's nucleotide sequence