Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4062

Experiment: sodium fluoride 12.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4060 and Pf1N1B4_4061 are separated by 68 nucleotidesPf1N1B4_4061 and Pf1N1B4_4062 are separated by 172 nucleotidesPf1N1B4_4062 and Pf1N1B4_4063 overlap by 1 nucleotidesPf1N1B4_4063 and Pf1N1B4_4064 are separated by 15 nucleotides Pf1N1B4_4060 - Cell division protein FtsK, at 4,513,027 to 4,515,432 _4060 Pf1N1B4_4061 - Outer membrane lipoprotein carrier protein LolA, at 4,515,501 to 4,516,124 _4061 Pf1N1B4_4062 - FIG065221: Holliday junction DNA helicase, at 4,516,297 to 4,517,619 _4062 Pf1N1B4_4063 - CrcB protein, at 4,517,619 to 4,517,993 _4063 Pf1N1B4_4064 - Seryl-tRNA synthetase (EC 6.1.1.11), at 4,518,009 to 4,519,289 _4064 Position (kb) 4516 4517 4518Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4515.488 kb on + strandat 4516.123 kb on - strandat 4516.136 kb on + strandat 4516.136 kb on + strandat 4516.136 kb on + strandat 4516.137 kb on - strandat 4516.137 kb on - strandat 4516.137 kb on - strandat 4516.175 kb on + strandat 4516.175 kb on + strandat 4516.175 kb on + strandat 4516.176 kb on - strandat 4516.288 kb on + strandat 4516.288 kb on + strandat 4516.288 kb on + strandat 4516.288 kb on + strandat 4516.288 kb on + strandat 4516.289 kb on - strandat 4516.289 kb on - strandat 4516.289 kb on - strandat 4516.293 kb on - strandat 4516.473 kb on + strand, within Pf1N1B4_4062at 4516.601 kb on - strand, within Pf1N1B4_4062at 4516.768 kb on + strand, within Pf1N1B4_4062at 4516.769 kb on - strand, within Pf1N1B4_4062at 4517.041 kb on + strand, within Pf1N1B4_4062at 4517.042 kb on - strand, within Pf1N1B4_4062at 4517.042 kb on - strand, within Pf1N1B4_4062at 4517.065 kb on - strand, within Pf1N1B4_4062at 4517.143 kb on + strand, within Pf1N1B4_4062at 4517.143 kb on + strand, within Pf1N1B4_4062at 4517.144 kb on - strand, within Pf1N1B4_4062at 4517.287 kb on - strand, within Pf1N1B4_4062at 4517.290 kb on + strand, within Pf1N1B4_4062at 4517.291 kb on - strand, within Pf1N1B4_4062at 4517.291 kb on - strand, within Pf1N1B4_4062at 4517.323 kb on + strand, within Pf1N1B4_4062at 4517.324 kb on - strand, within Pf1N1B4_4062at 4517.324 kb on - strand, within Pf1N1B4_4062at 4517.488 kb on + strandat 4517.489 kb on - strandat 4517.489 kb on - strandat 4517.489 kb on - strandat 4517.594 kb on + strandat 4517.595 kb on - strandat 4517.721 kb on + strand, within Pf1N1B4_4063at 4517.721 kb on + strand, within Pf1N1B4_4063at 4517.773 kb on + strand, within Pf1N1B4_4063at 4517.773 kb on + strand, within Pf1N1B4_4063at 4517.773 kb on + strand, within Pf1N1B4_4063at 4517.773 kb on + strand, within Pf1N1B4_4063at 4517.773 kb on + strand, within Pf1N1B4_4063at 4517.773 kb on + strand, within Pf1N1B4_4063at 4517.941 kb on + strand, within Pf1N1B4_4063

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Per-strain Table

Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM
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4,515,488 + -1.1
4,516,123 - -1.3
4,516,136 + +2.7
4,516,136 + +3.5
4,516,136 + +3.3
4,516,137 - -0.5
4,516,137 - -2.7
4,516,137 - -2.3
4,516,175 + +3.0
4,516,175 + +2.0
4,516,175 + +2.5
4,516,176 - +1.0
4,516,288 + -2.0
4,516,288 + -2.4
4,516,288 + -1.5
4,516,288 + -2.5
4,516,288 + -2.7
4,516,289 - -1.5
4,516,289 - -1.3
4,516,289 - +0.0
4,516,293 - -1.5
4,516,473 + Pf1N1B4_4062 0.13 -1.3
4,516,601 - Pf1N1B4_4062 0.23 -1.3
4,516,768 + Pf1N1B4_4062 0.36 -1.0
4,516,769 - Pf1N1B4_4062 0.36 -1.0
4,517,041 + Pf1N1B4_4062 0.56 -1.5
4,517,042 - Pf1N1B4_4062 0.56 -1.5
4,517,042 - Pf1N1B4_4062 0.56 -1.5
4,517,065 - Pf1N1B4_4062 0.58 -3.8
4,517,143 + Pf1N1B4_4062 0.64 +0.5
4,517,143 + Pf1N1B4_4062 0.64 -0.2
4,517,144 - Pf1N1B4_4062 0.64 -2.7
4,517,287 - Pf1N1B4_4062 0.75 -2.0
4,517,290 + Pf1N1B4_4062 0.75 -1.1
4,517,291 - Pf1N1B4_4062 0.75 -2.4
4,517,291 - Pf1N1B4_4062 0.75 -1.5
4,517,323 + Pf1N1B4_4062 0.78 -1.4
4,517,324 - Pf1N1B4_4062 0.78 -4.3
4,517,324 - Pf1N1B4_4062 0.78 -0.8
4,517,488 + +1.5
4,517,489 - -1.3
4,517,489 - -0.5
4,517,489 - -1.5
4,517,594 + -0.2
4,517,595 - -1.8
4,517,721 + Pf1N1B4_4063 0.27 -2.1
4,517,721 + Pf1N1B4_4063 0.27 -1.3
4,517,773 + Pf1N1B4_4063 0.41 -2.0
4,517,773 + Pf1N1B4_4063 0.41 -2.0
4,517,773 + Pf1N1B4_4063 0.41 +0.0
4,517,773 + Pf1N1B4_4063 0.41 -1.6
4,517,773 + Pf1N1B4_4063 0.41 -2.1
4,517,773 + Pf1N1B4_4063 0.41 -2.1
4,517,941 + Pf1N1B4_4063 0.86 -3.1

Or see this region's nucleotide sequence