Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3975 and Pf1N1B4_3976 are separated by 38 nucleotides Pf1N1B4_3976 and Pf1N1B4_3977 overlap by 4 nucleotides Pf1N1B4_3977 and Pf1N1B4_3978 are separated by 155 nucleotides
Pf1N1B4_3975 - Transcriptional regulator, IclR family, at 4,416,299 to 4,417,135
_3975
Pf1N1B4_3976 - Transmembrane regulator protein PrtR, at 4,417,174 to 4,417,926
_3976
Pf1N1B4_3977 - RNA polymerase sigma-54 factor RpoN, at 4,417,923 to 4,418,483
_3977
Pf1N1B4_3978 - Catalase (EC 1.11.1.6), at 4,418,639 to 4,419,688
_3978
Position (kb)
4417
4418 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4416.214 kb on + strand at 4416.233 kb on + strand at 4416.254 kb on - strand at 4416.812 kb on + strand at 4416.812 kb on + strand, within Pf1N1B4_3975 at 4416.845 kb on + strand, within Pf1N1B4_3975 at 4416.918 kb on + strand, within Pf1N1B4_3975 at 4416.919 kb on - strand, within Pf1N1B4_3975 at 4416.919 kb on - strand, within Pf1N1B4_3975 at 4417.238 kb on + strand at 4417.238 kb on + strand at 4417.238 kb on + strand at 4417.239 kb on - strand at 4417.239 kb on - strand at 4417.262 kb on + strand, within Pf1N1B4_3976 at 4417.318 kb on - strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand, within Pf1N1B4_3976 at 4417.337 kb on + strand at 4417.338 kb on - strand at 4417.370 kb on + strand, within Pf1N1B4_3976 at 4417.370 kb on + strand, within Pf1N1B4_3976 at 4417.370 kb on + strand at 4417.370 kb on + strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.371 kb on - strand, within Pf1N1B4_3976 at 4417.409 kb on - strand, within Pf1N1B4_3976 at 4417.498 kb on - strand, within Pf1N1B4_3976 at 4417.521 kb on - strand, within Pf1N1B4_3976 at 4417.521 kb on - strand, within Pf1N1B4_3976 at 4417.521 kb on - strand, within Pf1N1B4_3976 at 4417.838 kb on + strand, within Pf1N1B4_3976 at 4417.838 kb on + strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.839 kb on - strand, within Pf1N1B4_3976 at 4417.887 kb on - strand at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.051 kb on + strand, within Pf1N1B4_3977 at 4418.052 kb on - strand, within Pf1N1B4_3977 at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.053 kb on + strand at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.053 kb on + strand, within Pf1N1B4_3977 at 4418.054 kb on - strand, within Pf1N1B4_3977 at 4418.441 kb on + strand at 4418.449 kb on - strand at 4418.529 kb on + strand at 4418.598 kb on + strand at 4418.598 kb on + strand at 4418.598 kb on + strand at 4418.598 kb on + strand at 4418.599 kb on - strand at 4418.714 kb on + strand at 4418.791 kb on - strand, within Pf1N1B4_3978 at 4418.809 kb on + strand, within Pf1N1B4_3978
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 4,416,214 + +0.5 4,416,233 + -0.2 4,416,254 - -0.3 4,416,812 + +0.1 4,416,812 + Pf1N1B4_3975 0.61 -0.1 4,416,845 + Pf1N1B4_3975 0.65 +0.8 4,416,918 + Pf1N1B4_3975 0.74 +1.5 4,416,919 - Pf1N1B4_3975 0.74 +1.1 4,416,919 - Pf1N1B4_3975 0.74 +0.2 4,417,238 + +0.5 4,417,238 + -2.0 4,417,238 + +0.1 4,417,239 - -1.7 4,417,239 - +0.5 4,417,262 + Pf1N1B4_3976 0.12 -1.3 4,417,318 - Pf1N1B4_3976 0.19 +0.1 4,417,337 + Pf1N1B4_3976 0.22 +0.1 4,417,337 + Pf1N1B4_3976 0.22 -1.5 4,417,337 + Pf1N1B4_3976 0.22 -0.5 4,417,337 + Pf1N1B4_3976 0.22 +0.1 4,417,337 + +2.1 4,417,338 - +0.1 4,417,370 + Pf1N1B4_3976 0.26 -1.5 4,417,370 + Pf1N1B4_3976 0.26 +1.6 4,417,370 + -0.5 4,417,370 + Pf1N1B4_3976 0.26 -0.3 4,417,371 - Pf1N1B4_3976 0.26 -1.5 4,417,371 - +1.1 4,417,371 - Pf1N1B4_3976 0.26 -0.3 4,417,371 - Pf1N1B4_3976 0.26 -1.3 4,417,371 - Pf1N1B4_3976 0.26 -0.9 4,417,371 - Pf1N1B4_3976 0.26 -0.9 4,417,371 - Pf1N1B4_3976 0.26 -1.4 4,417,371 - Pf1N1B4_3976 0.26 -0.3 4,417,371 - Pf1N1B4_3976 0.26 +0.1 4,417,409 - Pf1N1B4_3976 0.31 -0.1 4,417,498 - Pf1N1B4_3976 0.43 -0.3 4,417,521 - Pf1N1B4_3976 0.46 -0.5 4,417,521 - Pf1N1B4_3976 0.46 +1.6 4,417,521 - Pf1N1B4_3976 0.46 -0.9 4,417,838 + Pf1N1B4_3976 0.88 +2.6 4,417,838 + Pf1N1B4_3976 0.88 -0.5 4,417,839 - Pf1N1B4_3976 0.88 -1.4 4,417,839 - Pf1N1B4_3976 0.88 -0.9 4,417,839 - Pf1N1B4_3976 0.88 -0.5 4,417,839 - Pf1N1B4_3976 0.88 +2.1 4,417,839 - Pf1N1B4_3976 0.88 -0.3 4,417,887 - +1.1 4,418,051 + Pf1N1B4_3977 0.23 +0.6 4,418,051 + Pf1N1B4_3977 0.23 -0.1 4,418,051 + Pf1N1B4_3977 0.23 +0.3 4,418,051 + Pf1N1B4_3977 0.23 -0.7 4,418,051 + Pf1N1B4_3977 0.23 +0.5 4,418,052 - Pf1N1B4_3977 0.23 -1.9 4,418,053 + Pf1N1B4_3977 0.23 -0.7 4,418,053 + -0.3 4,418,053 + Pf1N1B4_3977 0.23 +0.2 4,418,053 + Pf1N1B4_3977 0.23 -2.4 4,418,054 - Pf1N1B4_3977 0.23 -0.4 4,418,441 + +0.1 4,418,449 - -0.9 4,418,529 + -1.3 4,418,598 + +0.3 4,418,598 + -1.5 4,418,598 + +0.5 4,418,598 + +0.1 4,418,599 - +0.3 4,418,714 + -0.3 4,418,791 - Pf1N1B4_3978 0.14 +1.5 4,418,809 + Pf1N1B4_3978 0.16 +2.6
Or see this region's nucleotide sequence