Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3370 and Pf1N1B4_3371 are separated by 244 nucleotides Pf1N1B4_3371 and Pf1N1B4_3372 overlap by 13 nucleotides Pf1N1B4_3372 and Pf1N1B4_3373 overlap by 4 nucleotides Pf1N1B4_3373 and Pf1N1B4_3374 are separated by 126 nucleotides
Pf1N1B4_3370 - ATPase associated with various cellular activities, AAA_5, at 3,758,058 to 3,758,903
_3370
Pf1N1B4_3371 - FIG00954979: hypothetical protein, at 3,759,148 to 3,759,336
_3371
Pf1N1B4_3372 - FIG00962464: hypothetical protein, at 3,759,324 to 3,759,827
_3372
Pf1N1B4_3373 - FIG00954689: hypothetical protein, at 3,759,824 to 3,760,900
_3373
Pf1N1B4_3374 - Cysteine synthase (EC 2.5.1.47), at 3,761,027 to 3,762,001
_3374
Position (kb)
3759
3760
3761 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3758.878 kb on + strand at 3758.879 kb on - strand at 3758.879 kb on - strand at 3758.957 kb on + strand at 3758.957 kb on + strand at 3758.958 kb on - strand at 3758.958 kb on - strand at 3758.958 kb on - strand at 3758.963 kb on + strand at 3758.963 kb on + strand at 3758.964 kb on - strand at 3758.964 kb on - strand at 3758.964 kb on - strand at 3758.967 kb on - strand at 3758.974 kb on + strand at 3758.974 kb on + strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3759.013 kb on + strand at 3759.014 kb on - strand at 3759.014 kb on - strand at 3759.068 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.112 kb on - strand at 3759.112 kb on - strand at 3759.112 kb on - strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.119 kb on - strand at 3759.133 kb on - strand at 3759.328 kb on + strand at 3759.561 kb on + strand, within Pf1N1B4_3372 at 3759.561 kb on + strand, within Pf1N1B4_3372 at 3759.562 kb on - strand, within Pf1N1B4_3372 at 3759.589 kb on + strand, within Pf1N1B4_3372 at 3759.606 kb on - strand, within Pf1N1B4_3372 at 3759.683 kb on + strand at 3759.920 kb on - strand at 3760.108 kb on - strand, within Pf1N1B4_3373 at 3760.377 kb on + strand, within Pf1N1B4_3373 at 3760.377 kb on + strand, within Pf1N1B4_3373 at 3760.378 kb on - strand, within Pf1N1B4_3373 at 3760.378 kb on - strand, within Pf1N1B4_3373 at 3760.617 kb on + strand, within Pf1N1B4_3373 at 3760.748 kb on + strand at 3760.748 kb on + strand, within Pf1N1B4_3373 at 3760.748 kb on + strand, within Pf1N1B4_3373 at 3760.748 kb on + strand, within Pf1N1B4_3373 at 3760.818 kb on + strand at 3760.819 kb on - strand at 3760.888 kb on - strand at 3760.920 kb on + strand at 3760.923 kb on - strand at 3760.986 kb on + strand at 3760.986 kb on + strand at 3760.986 kb on + strand at 3760.986 kb on + strand at 3760.987 kb on - strand at 3760.987 kb on - strand at 3760.999 kb on + strand at 3760.999 kb on + strand at 3761.000 kb on - strand at 3761.000 kb on - strand at 3761.012 kb on + strand at 3761.012 kb on + strand at 3761.012 kb on + strand at 3761.035 kb on + strand at 3761.035 kb on + strand at 3761.036 kb on - strand at 3761.036 kb on - strand at 3761.036 kb on - strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.040 kb on - strand at 3761.040 kb on - strand at 3761.040 kb on - strand at 3761.068 kb on + strand at 3761.068 kb on + strand at 3761.069 kb on - strand at 3761.298 kb on + strand, within Pf1N1B4_3374 at 3761.444 kb on + strand, within Pf1N1B4_3374 at 3761.444 kb on + strand, within Pf1N1B4_3374 at 3761.445 kb on - strand, within Pf1N1B4_3374 at 3761.445 kb on - strand, within Pf1N1B4_3374 at 3761.445 kb on - strand at 3761.544 kb on + strand at 3761.544 kb on + strand, within Pf1N1B4_3374 at 3761.544 kb on + strand, within Pf1N1B4_3374 at 3761.545 kb on - strand, within Pf1N1B4_3374 at 3761.595 kb on - strand, within Pf1N1B4_3374 at 3761.596 kb on + strand at 3761.597 kb on - strand, within Pf1N1B4_3374 at 3761.843 kb on - strand, within Pf1N1B4_3374 at 3761.843 kb on - strand, within Pf1N1B4_3374
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 3,758,878 + -0.7 3,758,879 - -0.7 3,758,879 - +0.9 3,758,957 + +1.3 3,758,957 + -0.4 3,758,958 - +0.9 3,758,958 - -0.3 3,758,958 - -0.1 3,758,963 + +1.5 3,758,963 + +1.3 3,758,964 - +0.6 3,758,964 - +0.1 3,758,964 - -1.0 3,758,967 - -0.5 3,758,974 + +0.5 3,758,974 + +0.1 3,758,975 - -1.0 3,758,975 - -0.7 3,758,975 - -0.2 3,758,975 - +0.7 3,759,013 + -0.5 3,759,014 - -0.9 3,759,014 - +1.9 3,759,068 + -0.8 3,759,111 + +0.4 3,759,111 + -0.7 3,759,111 + -0.0 3,759,111 + -0.3 3,759,112 - +0.3 3,759,112 - +0.5 3,759,112 - -0.2 3,759,114 + +0.4 3,759,114 + -2.3 3,759,114 + +0.7 3,759,114 + -0.9 3,759,114 + -0.3 3,759,114 + +0.5 3,759,114 + +0.3 3,759,114 + -0.3 3,759,114 + +0.5 3,759,114 + -1.3 3,759,114 + -2.0 3,759,115 - -1.3 3,759,115 - +0.4 3,759,115 - -1.7 3,759,115 - -0.9 3,759,115 - +0.8 3,759,115 - -1.2 3,759,115 - -0.0 3,759,115 - +2.3 3,759,115 - +0.4 3,759,115 - +1.0 3,759,115 - +0.5 3,759,119 - -0.3 3,759,133 - -1.5 3,759,328 + +0.9 3,759,561 + Pf1N1B4_3372 0.47 +1.3 3,759,561 + Pf1N1B4_3372 0.47 +0.3 3,759,562 - Pf1N1B4_3372 0.47 -0.1 3,759,589 + Pf1N1B4_3372 0.53 -1.2 3,759,606 - Pf1N1B4_3372 0.56 +0.3 3,759,683 + +0.7 3,759,920 - +0.3 3,760,108 - Pf1N1B4_3373 0.26 -1.0 3,760,377 + Pf1N1B4_3373 0.51 -1.1 3,760,377 + Pf1N1B4_3373 0.51 -2.3 3,760,378 - Pf1N1B4_3373 0.51 -1.9 3,760,378 - Pf1N1B4_3373 0.51 +0.7 3,760,617 + Pf1N1B4_3373 0.74 -0.8 3,760,748 + -0.3 3,760,748 + Pf1N1B4_3373 0.86 -1.0 3,760,748 + Pf1N1B4_3373 0.86 -0.3 3,760,748 + Pf1N1B4_3373 0.86 -0.2 3,760,818 + -0.6 3,760,819 - +0.3 3,760,888 - +0.3 3,760,920 + -1.7 3,760,923 - +0.4 3,760,986 + -0.5 3,760,986 + -1.3 3,760,986 + +2.3 3,760,986 + +0.1 3,760,987 - +2.9 3,760,987 - +1.0 3,760,999 + -1.3 3,760,999 + +1.3 3,761,000 - +0.5 3,761,000 - -1.3 3,761,012 + -0.4 3,761,012 + -0.5 3,761,012 + -1.0 3,761,035 + -1.0 3,761,035 + +0.6 3,761,036 - +1.3 3,761,036 - -1.5 3,761,036 - -0.3 3,761,039 + +0.7 3,761,039 + +0.1 3,761,039 + -1.0 3,761,039 + +0.3 3,761,039 + -0.0 3,761,039 + -0.7 3,761,039 + -0.4 3,761,040 - +0.1 3,761,040 - -1.1 3,761,040 - -0.7 3,761,068 + -1.0 3,761,068 + +0.5 3,761,069 - -0.9 3,761,298 + Pf1N1B4_3374 0.28 +0.8 3,761,444 + Pf1N1B4_3374 0.43 -0.7 3,761,444 + Pf1N1B4_3374 0.43 -0.3 3,761,445 - Pf1N1B4_3374 0.43 +1.5 3,761,445 - Pf1N1B4_3374 0.43 +1.5 3,761,445 - -0.5 3,761,544 + -1.3 3,761,544 + Pf1N1B4_3374 0.53 -0.3 3,761,544 + Pf1N1B4_3374 0.53 -0.1 3,761,545 - Pf1N1B4_3374 0.53 +1.0 3,761,595 - Pf1N1B4_3374 0.58 +1.3 3,761,596 + +0.7 3,761,597 - Pf1N1B4_3374 0.58 -0.5 3,761,843 - Pf1N1B4_3374 0.84 +1.3 3,761,843 - Pf1N1B4_3374 0.84 +0.6
Or see this region's nucleotide sequence