Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3143 and Pf1N1B4_3144 are separated by 53 nucleotides Pf1N1B4_3144 and Pf1N1B4_3145 are separated by 43 nucleotides Pf1N1B4_3145 and Pf1N1B4_3146 are separated by 488 nucleotides Pf1N1B4_3146 and Pf1N1B4_3147 are separated by 0 nucleotides
Pf1N1B4_3143 - RecA protein, at 3,525,750 to 3,526,802
_3143
Pf1N1B4_3144 - Regulatory protein RecX, at 3,526,856 to 3,527,278
_3144
Pf1N1B4_3145 - Decarboxylase family protein, at 3,527,322 to 3,528,440
_3145
Pf1N1B4_3146 - FIG00957354: hypothetical protein, at 3,528,929 to 3,529,123
_3146
Pf1N1B4_3147 - Putative NADPH-quinone reductase (modulator of drug activity B), at 3,529,124 to 3,529,549
_3147
Position (kb)
3527
3528
3529 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3526.398 kb on + strand, within Pf1N1B4_3143 at 3526.398 kb on + strand, within Pf1N1B4_3143 at 3526.551 kb on + strand, within Pf1N1B4_3143 at 3526.551 kb on + strand, within Pf1N1B4_3143 at 3526.552 kb on - strand, within Pf1N1B4_3143 at 3526.617 kb on + strand, within Pf1N1B4_3143 at 3526.617 kb on + strand, within Pf1N1B4_3143 at 3526.822 kb on - strand at 3527.169 kb on + strand, within Pf1N1B4_3144 at 3527.292 kb on + strand at 3527.293 kb on - strand at 3527.293 kb on - strand at 3527.401 kb on + strand at 3527.401 kb on + strand at 3527.401 kb on + strand at 3527.402 kb on - strand at 3527.409 kb on + strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.504 kb on - strand, within Pf1N1B4_3145 at 3527.644 kb on + strand, within Pf1N1B4_3145 at 3527.683 kb on + strand, within Pf1N1B4_3145 at 3527.684 kb on - strand at 3527.684 kb on - strand, within Pf1N1B4_3145 at 3527.796 kb on + strand, within Pf1N1B4_3145 at 3527.796 kb on + strand, within Pf1N1B4_3145 at 3528.136 kb on + strand, within Pf1N1B4_3145 at 3528.136 kb on + strand, within Pf1N1B4_3145 at 3528.137 kb on - strand, within Pf1N1B4_3145 at 3528.137 kb on - strand, within Pf1N1B4_3145 at 3528.150 kb on + strand, within Pf1N1B4_3145 at 3528.586 kb on + strand at 3528.587 kb on - strand at 3528.617 kb on + strand at 3528.618 kb on - strand at 3528.618 kb on - strand at 3528.679 kb on + strand at 3528.679 kb on + strand at 3528.743 kb on + strand at 3528.744 kb on - strand at 3528.744 kb on - strand at 3528.791 kb on + strand at 3528.791 kb on + strand at 3528.791 kb on + strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.810 kb on + strand at 3528.813 kb on + strand at 3528.813 kb on + strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.818 kb on + strand at 3528.819 kb on - strand at 3528.819 kb on - strand at 3528.965 kb on + strand, within Pf1N1B4_3146 at 3529.058 kb on + strand, within Pf1N1B4_3146 at 3529.126 kb on - strand at 3529.293 kb on + strand, within Pf1N1B4_3147 at 3529.293 kb on + strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.436 kb on + strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 3,526,398 + Pf1N1B4_3143 0.62 -1.0 3,526,398 + Pf1N1B4_3143 0.62 +0.0 3,526,551 + Pf1N1B4_3143 0.76 -1.1 3,526,551 + Pf1N1B4_3143 0.76 +1.0 3,526,552 - Pf1N1B4_3143 0.76 -0.6 3,526,617 + Pf1N1B4_3143 0.82 -0.8 3,526,617 + Pf1N1B4_3143 0.82 -0.7 3,526,822 - -0.5 3,527,169 + Pf1N1B4_3144 0.74 -1.0 3,527,292 + -1.1 3,527,293 - +0.3 3,527,293 - -1.1 3,527,401 + -1.5 3,527,401 + +1.9 3,527,401 + +0.1 3,527,402 - +0.3 3,527,409 + -0.4 3,527,410 - +0.3 3,527,410 - -0.6 3,527,410 - -0.9 3,527,410 - +0.8 3,527,504 - Pf1N1B4_3145 0.16 -1.1 3,527,644 + Pf1N1B4_3145 0.29 -1.2 3,527,683 + Pf1N1B4_3145 0.32 -0.8 3,527,684 - +0.6 3,527,684 - Pf1N1B4_3145 0.32 -1.7 3,527,796 + Pf1N1B4_3145 0.42 -0.4 3,527,796 + Pf1N1B4_3145 0.42 -2.1 3,528,136 + Pf1N1B4_3145 0.73 +0.0 3,528,136 + Pf1N1B4_3145 0.73 -1.1 3,528,137 - Pf1N1B4_3145 0.73 +0.3 3,528,137 - Pf1N1B4_3145 0.73 +0.3 3,528,150 + Pf1N1B4_3145 0.74 +1.3 3,528,586 + +0.9 3,528,587 - +0.0 3,528,617 + +0.6 3,528,618 - -0.8 3,528,618 - +1.0 3,528,679 + +0.0 3,528,679 + -1.0 3,528,743 + -1.8 3,528,744 - +1.6 3,528,744 - +0.7 3,528,791 + +1.3 3,528,791 + +0.0 3,528,791 + -0.8 3,528,792 - -0.3 3,528,792 - +0.3 3,528,792 - +1.5 3,528,792 - +0.1 3,528,810 + +0.1 3,528,813 + +0.0 3,528,813 + +1.3 3,528,814 - +0.3 3,528,814 - +1.3 3,528,814 - +1.8 3,528,814 - -0.3 3,528,814 - +1.1 3,528,818 + -0.1 3,528,819 - -0.5 3,528,819 - +1.6 3,528,965 + Pf1N1B4_3146 0.18 +0.7 3,529,058 + Pf1N1B4_3146 0.66 -0.8 3,529,126 - +1.5 3,529,293 + Pf1N1B4_3147 0.40 +0.2 3,529,293 + Pf1N1B4_3147 0.40 +0.3 3,529,294 - Pf1N1B4_3147 0.40 +0.1 3,529,294 - Pf1N1B4_3147 0.40 +0.3 3,529,294 - Pf1N1B4_3147 0.40 -0.3 3,529,378 + Pf1N1B4_3147 0.60 +1.1 3,529,378 + Pf1N1B4_3147 0.60 -1.2 3,529,378 + Pf1N1B4_3147 0.60 +0.0 3,529,378 + Pf1N1B4_3147 0.60 -0.4 3,529,378 + Pf1N1B4_3147 0.60 -0.3 3,529,378 + Pf1N1B4_3147 0.60 -1.1 3,529,378 + Pf1N1B4_3147 0.60 +0.1 3,529,379 - Pf1N1B4_3147 0.60 +0.9 3,529,379 - Pf1N1B4_3147 0.60 -0.5 3,529,379 - Pf1N1B4_3147 0.60 -0.7 3,529,379 - Pf1N1B4_3147 0.60 -0.1 3,529,379 - Pf1N1B4_3147 0.60 -0.1 3,529,379 - Pf1N1B4_3147 0.60 -0.1 3,529,379 - Pf1N1B4_3147 0.60 -0.2 3,529,379 - Pf1N1B4_3147 0.60 -0.0 3,529,379 - Pf1N1B4_3147 0.60 +0.0 3,529,380 + Pf1N1B4_3147 0.60 -1.0 3,529,380 + Pf1N1B4_3147 0.60 -0.5 3,529,380 + Pf1N1B4_3147 0.60 -0.8 3,529,381 - Pf1N1B4_3147 0.60 -0.5 3,529,381 - Pf1N1B4_3147 0.60 -0.4 3,529,381 - Pf1N1B4_3147 0.60 -0.2 3,529,381 - Pf1N1B4_3147 0.60 -0.5 3,529,436 + Pf1N1B4_3147 0.73 -0.3 3,529,437 - Pf1N1B4_3147 0.73 -1.4 3,529,437 - Pf1N1B4_3147 0.73 +0.6 3,529,437 - Pf1N1B4_3147 0.73 +0.4 3,529,437 - Pf1N1B4_3147 0.73 -0.7 3,529,437 - Pf1N1B4_3147 0.73 -0.7
Or see this region's nucleotide sequence