Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2490 and Pf1N1B4_2491 overlap by 4 nucleotides Pf1N1B4_2491 and Pf1N1B4_2492 are separated by 120 nucleotides Pf1N1B4_2492 and Pf1N1B4_2493 are separated by 277 nucleotides
Pf1N1B4_2490 - Biotin synthesis protein BioC, at 2,823,076 to 2,823,888
_2490
Pf1N1B4_2491 - Dethiobiotin synthetase (EC 6.3.3.3), at 2,823,885 to 2,824,565
_2491
Pf1N1B4_2492 - FIG00954771: hypothetical protein, at 2,824,686 to 2,824,982
_2492
Pf1N1B4_2493 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 2,825,260 to 2,827,065
_2493
Position (kb)
2824
2825 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2823.694 kb on + strand, within Pf1N1B4_2490 at 2823.811 kb on + strand at 2823.811 kb on + strand at 2823.812 kb on - strand at 2823.812 kb on - strand at 2823.812 kb on - strand at 2823.850 kb on + strand at 2823.850 kb on + strand at 2823.850 kb on + strand at 2823.851 kb on - strand at 2823.884 kb on + strand at 2823.885 kb on - strand at 2823.885 kb on - strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.897 kb on + strand at 2823.898 kb on - strand at 2823.898 kb on - strand at 2823.898 kb on - strand at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.089 kb on + strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.090 kb on - strand, within Pf1N1B4_2491 at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.583 kb on + strand at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.730 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.880 kb on + strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.881 kb on - strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.906 kb on + strand, within Pf1N1B4_2492 at 2824.907 kb on - strand, within Pf1N1B4_2492 at 2824.907 kb on - strand at 2824.970 kb on + strand at 2824.971 kb on - strand at 2824.971 kb on - strand at 2825.038 kb on + strand at 2825.038 kb on + strand at 2825.038 kb on + strand at 2825.039 kb on - strand at 2825.039 kb on - strand at 2825.039 kb on - strand at 2825.057 kb on + strand at 2825.057 kb on + strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.058 kb on - strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.067 kb on + strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.068 kb on - strand at 2825.178 kb on + strand at 2825.178 kb on + strand at 2825.179 kb on - strand at 2825.179 kb on - strand at 2825.179 kb on - strand at 2825.237 kb on + strand at 2825.237 kb on + strand at 2825.260 kb on - strand at 2825.290 kb on - strand at 2825.506 kb on + strand, within Pf1N1B4_2493 at 2825.507 kb on - strand, within Pf1N1B4_2493 at 2825.552 kb on + strand, within Pf1N1B4_2493 at 2825.707 kb on + strand, within Pf1N1B4_2493 at 2825.824 kb on + strand, within Pf1N1B4_2493
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 2,823,694 + Pf1N1B4_2490 0.76 +0.0 2,823,811 + -0.4 2,823,811 + -0.4 2,823,812 - -0.4 2,823,812 - +0.0 2,823,812 - -0.4 2,823,850 + -0.0 2,823,850 + +0.6 2,823,850 + -0.7 2,823,851 - -1.2 2,823,884 + -0.4 2,823,885 - -0.1 2,823,885 - +2.0 2,823,897 + +0.6 2,823,897 + -2.2 2,823,897 + -0.6 2,823,897 + +0.9 2,823,897 + -1.0 2,823,898 - -0.2 2,823,898 - +0.9 2,823,898 - -2.0 2,824,089 + Pf1N1B4_2491 0.30 -1.1 2,824,089 + Pf1N1B4_2491 0.30 +0.3 2,824,089 + Pf1N1B4_2491 0.30 -0.6 2,824,089 + Pf1N1B4_2491 0.30 +0.9 2,824,090 - Pf1N1B4_2491 0.30 -1.0 2,824,090 - Pf1N1B4_2491 0.30 +0.8 2,824,090 - Pf1N1B4_2491 0.30 -0.3 2,824,090 - Pf1N1B4_2491 0.30 -0.2 2,824,090 - Pf1N1B4_2491 0.30 +0.4 2,824,583 + +0.4 2,824,583 + +0.4 2,824,583 + -1.8 2,824,730 + Pf1N1B4_2492 0.15 -1.2 2,824,730 + Pf1N1B4_2492 0.15 +0.0 2,824,880 + Pf1N1B4_2492 0.65 +0.6 2,824,880 + Pf1N1B4_2492 0.65 -0.1 2,824,881 - Pf1N1B4_2492 0.66 -0.6 2,824,881 - Pf1N1B4_2492 0.66 -1.2 2,824,881 - Pf1N1B4_2492 0.66 +0.3 2,824,906 + Pf1N1B4_2492 0.74 -0.4 2,824,906 + Pf1N1B4_2492 0.74 -1.7 2,824,906 + Pf1N1B4_2492 0.74 -0.2 2,824,906 + Pf1N1B4_2492 0.74 -0.8 2,824,906 + Pf1N1B4_2492 0.74 -0.1 2,824,907 - Pf1N1B4_2492 0.74 -1.5 2,824,907 - +0.6 2,824,970 + +0.6 2,824,971 - +1.0 2,824,971 - -0.2 2,825,038 + -1.4 2,825,038 + -1.2 2,825,038 + -1.4 2,825,039 - +0.3 2,825,039 - -0.6 2,825,039 - +0.9 2,825,057 + -0.1 2,825,057 + +1.6 2,825,058 - +0.0 2,825,058 - +0.5 2,825,058 - +1.3 2,825,058 - +0.6 2,825,067 + +0.6 2,825,067 + +0.6 2,825,067 + +0.6 2,825,067 + +0.2 2,825,067 + +0.0 2,825,068 - +0.0 2,825,068 - +2.2 2,825,068 - -1.6 2,825,068 - -0.6 2,825,068 - +0.6 2,825,178 + -0.5 2,825,178 + +0.0 2,825,179 - -0.6 2,825,179 - -1.2 2,825,179 - +0.2 2,825,237 + -2.1 2,825,237 + +1.6 2,825,260 - +0.0 2,825,290 - -0.2 2,825,506 + Pf1N1B4_2493 0.14 -0.2 2,825,507 - Pf1N1B4_2493 0.14 +0.6 2,825,552 + Pf1N1B4_2493 0.16 -1.2 2,825,707 + Pf1N1B4_2493 0.25 -1.6 2,825,824 + Pf1N1B4_2493 0.31 -0.7
Or see this region's nucleotide sequence