Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2348 and Pf1N1B4_2349 are separated by 99 nucleotides Pf1N1B4_2349 and Pf1N1B4_2350 overlap by 1 nucleotides Pf1N1B4_2350 and Pf1N1B4_2351 are separated by 190 nucleotides
Pf1N1B4_2348 - Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR, at 2,663,441 to 2,663,944
_2348
Pf1N1B4_2349 - Putative Holliday junction resolvase YggF, at 2,664,044 to 2,664,481
_2349
Pf1N1B4_2350 - UPF0301 protein YqgE, at 2,664,481 to 2,665,053
_2350
Pf1N1B4_2351 - Ferric siderophore transport system, periplasmic binding protein TonB, at 2,665,244 to 2,666,098
_2351
Position (kb)
2664
2665
2666 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2663.580 kb on - strand, within Pf1N1B4_2348 at 2663.580 kb on - strand, within Pf1N1B4_2348 at 2663.580 kb on - strand, within Pf1N1B4_2348 at 2663.608 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.854 kb on - strand, within Pf1N1B4_2348 at 2663.854 kb on - strand, within Pf1N1B4_2348 at 2663.886 kb on + strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2664.001 kb on - strand at 2664.001 kb on - strand at 2664.001 kb on - strand at 2664.005 kb on - strand at 2664.005 kb on - strand at 2664.035 kb on + strand at 2664.035 kb on + strand at 2664.035 kb on + strand at 2664.035 kb on + strand at 2664.035 kb on + strand at 2664.036 kb on - strand at 2664.036 kb on - strand at 2664.554 kb on - strand, within Pf1N1B4_2350 at 2664.554 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2665.061 kb on - strand at 2665.172 kb on + strand at 2665.172 kb on + strand at 2665.172 kb on + strand at 2665.173 kb on - strand at 2665.173 kb on - strand at 2665.249 kb on + strand at 2665.249 kb on + strand at 2665.250 kb on - strand at 2665.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 2,663,580 - Pf1N1B4_2348 0.28 -0.0 2,663,580 - Pf1N1B4_2348 0.28 -0.0 2,663,580 - Pf1N1B4_2348 0.28 -0.2 2,663,608 - Pf1N1B4_2348 0.33 -0.2 2,663,718 - Pf1N1B4_2348 0.55 +1.1 2,663,718 - Pf1N1B4_2348 0.55 +0.1 2,663,718 - Pf1N1B4_2348 0.55 -0.6 2,663,718 - Pf1N1B4_2348 0.55 -1.6 2,663,718 - Pf1N1B4_2348 0.55 -0.5 2,663,718 - Pf1N1B4_2348 0.55 +1.0 2,663,718 - Pf1N1B4_2348 0.55 +1.4 2,663,718 - Pf1N1B4_2348 0.55 +0.1 2,663,718 - Pf1N1B4_2348 0.55 -2.1 2,663,854 - Pf1N1B4_2348 0.82 -0.6 2,663,854 - Pf1N1B4_2348 0.82 -0.9 2,663,886 + Pf1N1B4_2348 0.88 -0.3 2,663,887 - Pf1N1B4_2348 0.88 -0.8 2,663,887 - Pf1N1B4_2348 0.88 -0.7 2,663,887 - Pf1N1B4_2348 0.88 +2.3 2,663,887 - Pf1N1B4_2348 0.88 +1.1 2,663,887 - Pf1N1B4_2348 0.88 -0.5 2,663,887 - Pf1N1B4_2348 0.88 -1.2 2,663,887 - Pf1N1B4_2348 0.88 -0.1 2,663,887 - Pf1N1B4_2348 0.88 +0.3 2,663,887 - Pf1N1B4_2348 0.88 -1.0 2,663,887 - Pf1N1B4_2348 0.88 +0.4 2,663,887 - Pf1N1B4_2348 0.88 +1.7 2,663,887 - Pf1N1B4_2348 0.88 +0.3 2,664,001 - -0.8 2,664,001 - +1.7 2,664,001 - -1.9 2,664,005 - -0.5 2,664,005 - -2.1 2,664,035 + +1.3 2,664,035 + -1.1 2,664,035 + -0.8 2,664,035 + +0.7 2,664,035 + +0.1 2,664,036 - -0.4 2,664,036 - -0.0 2,664,554 - Pf1N1B4_2350 0.13 +0.7 2,664,554 - Pf1N1B4_2350 0.13 -1.5 2,664,963 - Pf1N1B4_2350 0.84 +0.2 2,664,963 - Pf1N1B4_2350 0.84 -0.1 2,664,963 - Pf1N1B4_2350 0.84 -1.1 2,664,963 - Pf1N1B4_2350 0.84 -1.1 2,664,963 - Pf1N1B4_2350 0.84 +3.7 2,664,963 - Pf1N1B4_2350 0.84 -0.6 2,664,963 - Pf1N1B4_2350 0.84 -0.6 2,664,963 - Pf1N1B4_2350 0.84 -0.9 2,665,061 - -1.1 2,665,172 + -0.3 2,665,172 + +0.9 2,665,172 + -0.9 2,665,173 - +0.6 2,665,173 - -0.4 2,665,249 + +0.3 2,665,249 + -1.5 2,665,250 - -0.6 2,665,250 - +0.7
Or see this region's nucleotide sequence