Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2350

Experiment: sodium fluoride 12.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2348 and Pf1N1B4_2349 are separated by 99 nucleotidesPf1N1B4_2349 and Pf1N1B4_2350 overlap by 1 nucleotidesPf1N1B4_2350 and Pf1N1B4_2351 are separated by 190 nucleotides Pf1N1B4_2348 - Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR, at 2,663,441 to 2,663,944 _2348 Pf1N1B4_2349 - Putative Holliday junction resolvase YggF, at 2,664,044 to 2,664,481 _2349 Pf1N1B4_2350 - UPF0301 protein YqgE, at 2,664,481 to 2,665,053 _2350 Pf1N1B4_2351 - Ferric siderophore transport system, periplasmic binding protein TonB, at 2,665,244 to 2,666,098 _2351 Position (kb) 2664 2665 2666Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2663.580 kb on - strand, within Pf1N1B4_2348at 2663.580 kb on - strand, within Pf1N1B4_2348at 2663.580 kb on - strand, within Pf1N1B4_2348at 2663.608 kb on - strand, within Pf1N1B4_2348at 2663.718 kb on - strand, within Pf1N1B4_2348at 2663.718 kb on - strand, within Pf1N1B4_2348at 2663.718 kb on - strand, within Pf1N1B4_2348at 2663.718 kb on - strand, within Pf1N1B4_2348at 2663.718 kb on - strand, within Pf1N1B4_2348at 2663.718 kb on - strand, within Pf1N1B4_2348at 2663.718 kb on - strand, within Pf1N1B4_2348at 2663.718 kb on - strand, within Pf1N1B4_2348at 2663.718 kb on - strand, within Pf1N1B4_2348at 2663.854 kb on - strand, within Pf1N1B4_2348at 2663.854 kb on - strand, within Pf1N1B4_2348at 2663.886 kb on + strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2663.887 kb on - strand, within Pf1N1B4_2348at 2664.001 kb on - strandat 2664.001 kb on - strandat 2664.001 kb on - strandat 2664.005 kb on - strandat 2664.005 kb on - strandat 2664.035 kb on + strandat 2664.035 kb on + strandat 2664.035 kb on + strandat 2664.035 kb on + strandat 2664.035 kb on + strandat 2664.036 kb on - strandat 2664.036 kb on - strandat 2664.554 kb on - strand, within Pf1N1B4_2350at 2664.554 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2664.963 kb on - strand, within Pf1N1B4_2350at 2665.061 kb on - strandat 2665.172 kb on + strandat 2665.172 kb on + strandat 2665.172 kb on + strandat 2665.173 kb on - strandat 2665.173 kb on - strandat 2665.249 kb on + strandat 2665.249 kb on + strandat 2665.250 kb on - strandat 2665.250 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM
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2,663,580 - Pf1N1B4_2348 0.28 -0.0
2,663,580 - Pf1N1B4_2348 0.28 -0.0
2,663,580 - Pf1N1B4_2348 0.28 -0.2
2,663,608 - Pf1N1B4_2348 0.33 -0.2
2,663,718 - Pf1N1B4_2348 0.55 +1.1
2,663,718 - Pf1N1B4_2348 0.55 +0.1
2,663,718 - Pf1N1B4_2348 0.55 -0.6
2,663,718 - Pf1N1B4_2348 0.55 -1.6
2,663,718 - Pf1N1B4_2348 0.55 -0.5
2,663,718 - Pf1N1B4_2348 0.55 +1.0
2,663,718 - Pf1N1B4_2348 0.55 +1.4
2,663,718 - Pf1N1B4_2348 0.55 +0.1
2,663,718 - Pf1N1B4_2348 0.55 -2.1
2,663,854 - Pf1N1B4_2348 0.82 -0.6
2,663,854 - Pf1N1B4_2348 0.82 -0.9
2,663,886 + Pf1N1B4_2348 0.88 -0.3
2,663,887 - Pf1N1B4_2348 0.88 -0.8
2,663,887 - Pf1N1B4_2348 0.88 -0.7
2,663,887 - Pf1N1B4_2348 0.88 +2.3
2,663,887 - Pf1N1B4_2348 0.88 +1.1
2,663,887 - Pf1N1B4_2348 0.88 -0.5
2,663,887 - Pf1N1B4_2348 0.88 -1.2
2,663,887 - Pf1N1B4_2348 0.88 -0.1
2,663,887 - Pf1N1B4_2348 0.88 +0.3
2,663,887 - Pf1N1B4_2348 0.88 -1.0
2,663,887 - Pf1N1B4_2348 0.88 +0.4
2,663,887 - Pf1N1B4_2348 0.88 +1.7
2,663,887 - Pf1N1B4_2348 0.88 +0.3
2,664,001 - -0.8
2,664,001 - +1.7
2,664,001 - -1.9
2,664,005 - -0.5
2,664,005 - -2.1
2,664,035 + +1.3
2,664,035 + -1.1
2,664,035 + -0.8
2,664,035 + +0.7
2,664,035 + +0.1
2,664,036 - -0.4
2,664,036 - -0.0
2,664,554 - Pf1N1B4_2350 0.13 +0.7
2,664,554 - Pf1N1B4_2350 0.13 -1.5
2,664,963 - Pf1N1B4_2350 0.84 +0.2
2,664,963 - Pf1N1B4_2350 0.84 -0.1
2,664,963 - Pf1N1B4_2350 0.84 -1.1
2,664,963 - Pf1N1B4_2350 0.84 -1.1
2,664,963 - Pf1N1B4_2350 0.84 +3.7
2,664,963 - Pf1N1B4_2350 0.84 -0.6
2,664,963 - Pf1N1B4_2350 0.84 -0.6
2,664,963 - Pf1N1B4_2350 0.84 -0.9
2,665,061 - -1.1
2,665,172 + -0.3
2,665,172 + +0.9
2,665,172 + -0.9
2,665,173 - +0.6
2,665,173 - -0.4
2,665,249 + +0.3
2,665,249 + -1.5
2,665,250 - -0.6
2,665,250 - +0.7

Or see this region's nucleotide sequence