Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1659 and Pf1N1B4_1660 are separated by 114 nucleotides Pf1N1B4_1660 and Pf1N1B4_1661 are separated by 245 nucleotides Pf1N1B4_1661 and Pf1N1B4_1662 are separated by 3 nucleotides
Pf1N1B4_1659 - Ferrichrome-iron receptor, at 1,899,182 to 1,901,275
_1659
Pf1N1B4_1660 - FIG00953324: hypothetical protein, at 1,901,390 to 1,901,602
_1660
Pf1N1B4_1661 - Agmatine deiminase (EC 3.5.3.12), at 1,901,848 to 1,902,954
_1661
Pf1N1B4_1662 - N-carbamoylputrescine amidase (3.5.1.53) / Aliphatic amidase AmiE (EC 3.5.1.4), at 1,902,958 to 1,903,836
_1662
Position (kb)
1901
1902
1903 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1900.901 kb on + strand, within Pf1N1B4_1659 at 1900.901 kb on + strand, within Pf1N1B4_1659 at 1900.901 kb on + strand, within Pf1N1B4_1659 at 1900.901 kb on + strand, within Pf1N1B4_1659 at 1900.902 kb on - strand, within Pf1N1B4_1659 at 1900.902 kb on - strand, within Pf1N1B4_1659 at 1900.902 kb on - strand, within Pf1N1B4_1659 at 1900.902 kb on - strand, within Pf1N1B4_1659 at 1901.193 kb on - strand at 1901.193 kb on - strand at 1901.195 kb on + strand at 1901.196 kb on - strand at 1901.196 kb on - strand at 1901.261 kb on - strand at 1901.261 kb on - strand at 1901.264 kb on + strand at 1901.265 kb on - strand at 1901.265 kb on - strand at 1901.265 kb on - strand at 1901.265 kb on - strand at 1901.273 kb on + strand at 1901.274 kb on - strand at 1901.322 kb on - strand at 1901.337 kb on + strand at 1901.337 kb on + strand at 1901.338 kb on - strand at 1901.368 kb on + strand at 1901.368 kb on + strand at 1901.369 kb on - strand at 1901.369 kb on - strand at 1901.392 kb on - strand at 1901.392 kb on - strand at 1901.392 kb on - strand at 1901.546 kb on - strand, within Pf1N1B4_1660 at 1901.548 kb on + strand, within Pf1N1B4_1660 at 1901.548 kb on + strand, within Pf1N1B4_1660 at 1901.548 kb on + strand, within Pf1N1B4_1660 at 1901.548 kb on + strand, within Pf1N1B4_1660 at 1901.548 kb on + strand, within Pf1N1B4_1660 at 1901.549 kb on - strand, within Pf1N1B4_1660 at 1901.549 kb on - strand, within Pf1N1B4_1660 at 1901.549 kb on - strand, within Pf1N1B4_1660 at 1901.549 kb on - strand, within Pf1N1B4_1660 at 1901.549 kb on - strand, within Pf1N1B4_1660 at 1901.638 kb on + strand at 1901.638 kb on + strand at 1901.638 kb on + strand at 1901.638 kb on + strand at 1901.638 kb on + strand at 1901.649 kb on + strand at 1901.650 kb on - strand at 1901.699 kb on + strand at 1901.699 kb on + strand at 1901.700 kb on - strand at 1901.700 kb on - strand at 1901.796 kb on + strand at 1901.796 kb on + strand at 1901.797 kb on - strand at 1901.837 kb on + strand at 1901.838 kb on - strand at 1901.838 kb on - strand at 1902.044 kb on + strand, within Pf1N1B4_1661 at 1902.045 kb on - strand, within Pf1N1B4_1661 at 1902.045 kb on - strand, within Pf1N1B4_1661 at 1902.045 kb on - strand, within Pf1N1B4_1661 at 1902.045 kb on - strand, within Pf1N1B4_1661 at 1902.266 kb on - strand, within Pf1N1B4_1661 at 1902.266 kb on - strand, within Pf1N1B4_1661 at 1902.266 kb on - strand, within Pf1N1B4_1661 at 1902.266 kb on - strand, within Pf1N1B4_1661 at 1902.285 kb on - strand, within Pf1N1B4_1661 at 1902.349 kb on - strand, within Pf1N1B4_1661 at 1902.349 kb on - strand, within Pf1N1B4_1661 at 1902.349 kb on - strand, within Pf1N1B4_1661 at 1902.349 kb on - strand, within Pf1N1B4_1661 at 1902.670 kb on - strand, within Pf1N1B4_1661 at 1902.915 kb on - strand at 1902.915 kb on - strand at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.159 kb on - strand, within Pf1N1B4_1662 at 1903.446 kb on - strand, within Pf1N1B4_1662 at 1903.446 kb on - strand, within Pf1N1B4_1662 at 1903.448 kb on + strand, within Pf1N1B4_1662 at 1903.489 kb on - strand, within Pf1N1B4_1662 at 1903.494 kb on - strand, within Pf1N1B4_1662 at 1903.566 kb on - strand, within Pf1N1B4_1662 at 1903.566 kb on - strand, within Pf1N1B4_1662 at 1903.566 kb on - strand, within Pf1N1B4_1662 at 1903.661 kb on + strand, within Pf1N1B4_1662 at 1903.662 kb on - strand, within Pf1N1B4_1662 at 1903.689 kb on - strand, within Pf1N1B4_1662
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 1,900,901 + Pf1N1B4_1659 0.82 -0.1 1,900,901 + Pf1N1B4_1659 0.82 -0.0 1,900,901 + Pf1N1B4_1659 0.82 +0.7 1,900,901 + Pf1N1B4_1659 0.82 +0.4 1,900,902 - Pf1N1B4_1659 0.82 -1.4 1,900,902 - Pf1N1B4_1659 0.82 +0.3 1,900,902 - Pf1N1B4_1659 0.82 +0.2 1,900,902 - Pf1N1B4_1659 0.82 -1.7 1,901,193 - +0.7 1,901,193 - +0.1 1,901,195 + +1.1 1,901,196 - -0.0 1,901,196 - -0.0 1,901,261 - -2.1 1,901,261 - -0.4 1,901,264 + +1.2 1,901,265 - -0.3 1,901,265 - -1.2 1,901,265 - -0.9 1,901,265 - -0.3 1,901,273 + -1.4 1,901,274 - -0.1 1,901,322 - -2.1 1,901,337 + +0.5 1,901,337 + +0.7 1,901,338 - +0.6 1,901,368 + -1.3 1,901,368 + -0.9 1,901,369 - +0.6 1,901,369 - -0.2 1,901,392 - -1.4 1,901,392 - +0.7 1,901,392 - -1.1 1,901,546 - Pf1N1B4_1660 0.73 +1.5 1,901,548 + Pf1N1B4_1660 0.74 -1.4 1,901,548 + Pf1N1B4_1660 0.74 -0.6 1,901,548 + Pf1N1B4_1660 0.74 -0.4 1,901,548 + Pf1N1B4_1660 0.74 +0.0 1,901,548 + Pf1N1B4_1660 0.74 -0.3 1,901,549 - Pf1N1B4_1660 0.75 -1.6 1,901,549 - Pf1N1B4_1660 0.75 +0.9 1,901,549 - Pf1N1B4_1660 0.75 +1.7 1,901,549 - Pf1N1B4_1660 0.75 -1.3 1,901,549 - Pf1N1B4_1660 0.75 -0.3 1,901,638 + +0.2 1,901,638 + -1.3 1,901,638 + -0.2 1,901,638 + -0.5 1,901,638 + +0.9 1,901,649 + +0.1 1,901,650 - -0.4 1,901,699 + +0.3 1,901,699 + +0.1 1,901,700 - -0.3 1,901,700 - +1.5 1,901,796 + -0.4 1,901,796 + +1.0 1,901,797 - +3.7 1,901,837 + -1.4 1,901,838 - -1.2 1,901,838 - -0.4 1,902,044 + Pf1N1B4_1661 0.18 +0.1 1,902,045 - Pf1N1B4_1661 0.18 -0.9 1,902,045 - Pf1N1B4_1661 0.18 -1.2 1,902,045 - Pf1N1B4_1661 0.18 -2.5 1,902,045 - Pf1N1B4_1661 0.18 +0.0 1,902,266 - Pf1N1B4_1661 0.38 -1.4 1,902,266 - Pf1N1B4_1661 0.38 +1.1 1,902,266 - Pf1N1B4_1661 0.38 -1.3 1,902,266 - Pf1N1B4_1661 0.38 -0.4 1,902,285 - Pf1N1B4_1661 0.39 -0.5 1,902,349 - Pf1N1B4_1661 0.45 -0.4 1,902,349 - Pf1N1B4_1661 0.45 -0.3 1,902,349 - Pf1N1B4_1661 0.45 -2.2 1,902,349 - Pf1N1B4_1661 0.45 +0.1 1,902,670 - Pf1N1B4_1661 0.74 +0.1 1,902,915 - -0.3 1,902,915 - -1.3 1,903,159 - Pf1N1B4_1662 0.23 -0.4 1,903,159 - Pf1N1B4_1662 0.23 +0.9 1,903,159 - Pf1N1B4_1662 0.23 +0.1 1,903,159 - Pf1N1B4_1662 0.23 -1.1 1,903,159 - Pf1N1B4_1662 0.23 -0.6 1,903,159 - Pf1N1B4_1662 0.23 +1.4 1,903,446 - Pf1N1B4_1662 0.56 +0.3 1,903,446 - Pf1N1B4_1662 0.56 -1.3 1,903,448 + Pf1N1B4_1662 0.56 -0.3 1,903,489 - Pf1N1B4_1662 0.60 -1.4 1,903,494 - Pf1N1B4_1662 0.61 -0.5 1,903,566 - Pf1N1B4_1662 0.69 -0.4 1,903,566 - Pf1N1B4_1662 0.69 +0.1 1,903,566 - Pf1N1B4_1662 0.69 +0.7 1,903,661 + Pf1N1B4_1662 0.80 -0.2 1,903,662 - Pf1N1B4_1662 0.80 +1.7 1,903,689 - Pf1N1B4_1662 0.83 -0.5
Or see this region's nucleotide sequence