Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_157

Experiment: sodium fluoride 12.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_156 and Pf1N1B4_157 are separated by 91 nucleotidesPf1N1B4_157 and Pf1N1B4_158 are separated by 37 nucleotidesPf1N1B4_158 and Pf1N1B4_159 are separated by 26 nucleotides Pf1N1B4_156 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 198,975 to 199,967 _156 Pf1N1B4_157 - ThiJ/PfpI family protein, at 200,059 to 200,745 _157 Pf1N1B4_158 - FIG00456158: hypothetical protein, at 200,783 to 201,112 _158 Pf1N1B4_159 - Putative threonine efflux protein, at 201,139 to 201,768 _159 Position (kb) 200 201Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 199.531 kb on - strand, within Pf1N1B4_156at 199.968 kb on - strandat 199.968 kb on - strandat 200.012 kb on - strandat 200.019 kb on + strandat 200.020 kb on - strandat 200.020 kb on - strandat 200.020 kb on - strandat 200.122 kb on + strandat 200.123 kb on - strandat 200.123 kb on - strandat 200.123 kb on - strandat 200.345 kb on - strand, within Pf1N1B4_157at 200.345 kb on - strand, within Pf1N1B4_157at 200.362 kb on - strand, within Pf1N1B4_157at 200.599 kb on + strand, within Pf1N1B4_157at 200.832 kb on + strand, within Pf1N1B4_158at 200.833 kb on - strand, within Pf1N1B4_158at 200.833 kb on - strandat 200.833 kb on - strand, within Pf1N1B4_158at 200.935 kb on + strand, within Pf1N1B4_158at 200.935 kb on + strand, within Pf1N1B4_158at 200.956 kb on - strandat 200.959 kb on - strand, within Pf1N1B4_158at 200.959 kb on - strand, within Pf1N1B4_158at 200.959 kb on - strand, within Pf1N1B4_158at 201.090 kb on + strandat 201.091 kb on - strandat 201.095 kb on - strandat 201.108 kb on + strandat 201.108 kb on + strandat 201.108 kb on + strandat 201.108 kb on + strandat 201.108 kb on + strandat 201.109 kb on - strandat 201.109 kb on - strandat 201.231 kb on + strandat 201.255 kb on + strand, within Pf1N1B4_159at 201.256 kb on - strand, within Pf1N1B4_159at 201.392 kb on - strand, within Pf1N1B4_159at 201.665 kb on + strand, within Pf1N1B4_159at 201.666 kb on - strand, within Pf1N1B4_159

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Per-strain Table

Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM
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199,531 - Pf1N1B4_156 0.56 +0.7
199,968 - -1.5
199,968 - -3.1
200,012 - +0.1
200,019 + -2.5
200,020 - +0.7
200,020 - -0.3
200,020 - +0.3
200,122 + -1.1
200,123 - -1.2
200,123 - -0.9
200,123 - +0.5
200,345 - Pf1N1B4_157 0.42 -0.2
200,345 - Pf1N1B4_157 0.42 -1.2
200,362 - Pf1N1B4_157 0.44 -1.6
200,599 + Pf1N1B4_157 0.79 +0.1
200,832 + Pf1N1B4_158 0.15 -0.6
200,833 - Pf1N1B4_158 0.15 +0.8
200,833 - +3.1
200,833 - Pf1N1B4_158 0.15 +0.2
200,935 + Pf1N1B4_158 0.46 -0.7
200,935 + Pf1N1B4_158 0.46 -0.7
200,956 - +0.5
200,959 - Pf1N1B4_158 0.53 +0.3
200,959 - Pf1N1B4_158 0.53 +1.2
200,959 - Pf1N1B4_158 0.53 +0.8
201,090 + -0.5
201,091 - +0.1
201,095 - -0.9
201,108 + +0.1
201,108 + -0.5
201,108 + -0.7
201,108 + -0.8
201,108 + -0.2
201,109 - +1.7
201,109 - -1.2
201,231 + -0.5
201,255 + Pf1N1B4_159 0.18 -0.8
201,256 - Pf1N1B4_159 0.19 +1.1
201,392 - Pf1N1B4_159 0.40 -1.0
201,665 + Pf1N1B4_159 0.83 +0.3
201,666 - Pf1N1B4_159 0.84 -1.2

Or see this region's nucleotide sequence